Article
Chemistry, Applied
Xiaoyuan Wang, Yike Han, Junjun Geng, Amei Zhu, Xiaopeng Wei, Qisen Xiang, Wei Zong
Summary: Cold plasma can effectively remove the two mycotoxins produced by Alternaria fungi. Proteins and polyphenols in food components have a significant impact on toxin removal, while organic acids, Vc, sugars, and amino acids have little effect.
Article
Microbiology
Liuqing Wang, Meng Wang, Jian Jiao, Hongmei Liu
Summary: This study investigated the regulatory roles of AaVeA in A. alternata with various light sources. The results showed that AaVeA plays an important role in fungal growth, development, mycotoxin biosynthesis, and pathogenicity in response to different light sources.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Huikang Lin, Bingxuan Jia, Aibo Wu
Summary: In this study, the single and combined toxicities of Alternariol (AOH), alternariol monomethyl ether (AME), and tenuazonic acid (TeA) were analyzed using the human gastric epithelial cell line (GES-1). The results showed that AOH + AME + TeA exhibited synergistic toxic effects and induced apoptosis and DNA damage pathways.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Microbiology
Ahmed Mahmoud Ismail, Eman Said Elshewy, Sherif Mohamed El-Ganainy, Donato Magista, Ahlam Farouk Hamouda, Khalid A. Alhudaib, Weaam Ebrahim, Mustafa I. Almaghasla
Summary: This study aimed to explore the cytotoxicity and genotoxicity of mycotoxins produced by pathogenic A. alternata from tomatoes. The mycotoxins ALT, AOH, TeA, and AS exhibited cytotoxic and genotoxic effects on human oral epithelial cells, lung fibroblast cells, and male albino rats. The pathogenic potentiality and mycotoxin production ability of A. alternata were also investigated.
Article
Microbiology
Bo Li, Yuhan Cao, Zixuan Ji, Jingyi Zhang, Xianglong Meng, Pengbo Dai, Tongle Hu, Shutong Wang, Keqiang Cao, Yanan Wang
Summary: This study describes the association of two mycoviruses with hypovirulence in Alternaria alternata apple pathotype, where AaCV1-QY2 plays a leading role. The presence of these mycoviruses reduces host virulence and accumulation of the mycotoxin alternariol. Additionally, these mycoviruses can be horizontally transmitted to other A. alternata strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Shuai Meng, Suya Huang, Jinhua Liu, Yunpeng Gai, Min Li, Shuo Duan, Shuting Zhang, Xuepeng Sun, Qi Yang, Yuchun Wang, Kai Xu, Haijie Ma
Summary: Histone methylation plays a critical role in transcriptional regulation and biological processes in eukaryotes. This study investigated the function of several histone methyltransferases (HMTs) and histone demethylases (HDMs) in the phytopathogenic fungus Alternaria alternata. The results showed that AaSet1 and AaSet2 are important for cell development, stress adaptation, and carbon source utilization in A. alternata. RNA-seq analysis revealed the regulatory mechanism of AaSet1 and AaSet2. Overall, this study provides genetic evidence for the central role of HMTs and HDMs in the functions of A. alternata.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Yan Guo, Xue Li, Canying Li, Ruxin Jinyue, Hengping Xu, Yonghong Ge
Summary: This study found that Acibenzolar-S-methyl (ASM) can significantly reduce the lesion diameter of black spot rot on pears and enhance vital enzyme activity and gene expression in the phenylpropanoid pathway, thereby enhancing resistance against the causal agent. Furthermore, ASM also increased the content of various secondary metabolites in pears.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Horticulture
Liuyi Pan, Xurui Chen, Wei Xu, Shangshu Fan, Tian Wan, Juan Zhang, Yuliang Cai
Summary: The study found that methyl jasmonate (MeJA) had a minimal impact on the mycelial growth of Alternaria alternata in vitro at lower concentrations, but significantly reduced decay and enhanced antioxidant enzyme activities in sweet cherry fruits when applied at an optimal concentration. MeJA treatment also up-regulated the expression of key genes related to jasmonic acid biosynthesis and signal transduction pathways, suggesting a mechanism for inducing sweet cherry's resistance to A. alternata.
SCIENTIA HORTICULTURAE
(2022)
Article
Microbiology
Rebecca Creamer, Deana Baucom Hille, Marwa Neyaz, Tesneem Nusayr, Christopher L. Schardl, Daniel Cook
Summary: The fungal endophyte Alternaria oxytropis in the legume Oxytropis sericea produces secondary metabolites like the toxin swainsonine. Through genome analysis, 13 PKS, 5 NRPS, and 4 PKS-NRPS hybrids were identified, involved in biosynthesis of swainsonine and melanin. Phylogenetic analysis revealed evolutionary differences in some PKS and NRPS genes compared to fungi in Pleosporales and Dothideomycetes.
Article
Biotechnology & Applied Microbiology
Qianqian Jiang, Renyan Mao, Yongcai Li, Yang Bi, Yongxiang Liu, Miao Zhang, Rong Li, Yangyang Yang, Dov B. Prusky
Summary: This study investigated the characteristics and functions of AaCaM in Alternaria alternata, a pathogen causing pear black spot. The results showed that AaCaM plays an important role in regulating infection structure differentiation and secondary metabolism of A. alternata. The findings provide a theoretical basis for further understanding the specific role of AaCaM in the calcium signaling pathway and pathogenicity of A. alternata.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Education, Scientific Disciplines
Randa Abdou, Gouda H. Attia, Mariam Mojally, Mohamed Dawoud, Mostafa E. Rateb
Summary: Alternaria alternata, isolated from the leaves of Ficus carica, showed promising antifungal and cytotoxic activities. Four alternariol derivatives were isolated and identified. Alternariol exhibited the highest antifungal activity against A. terreus and F. oxysporum, while alternariol-5-O-methyl ether showed the highest cytotoxicity against K-562 and HUVEC cell lines.
INDIAN JOURNAL OF PHARMACEUTICAL EDUCATION AND RESEARCH
(2022)
Article
Chemistry, Medicinal
Qingqing Peng, Yongqi Li, Jiasong Fang, Xi Yu
Summary: The study focuses on the isolation and characterization of hadal fungi, screening for potential strains with bioactive natural products. The results demonstrate the significant effects of chemical epigenetic modifiers on gene expression, production of secondary metabolites, and antimicrobial activity of hadal fungi. High hydrostatic pressure conditions also activate the production of new compounds in hadal fungi.
Article
Microbiology
Haijie Ma, Lei Li, Yunpeng Gai, Xiaoyan Zhang, Yanan Chen, Xiaokang Zhuo, Yingzi Cao, Chen Jiao, Fred G. Gmitter, Hongye Li
Summary: This study revealed the essential roles of HATs and HDACs in growth, pathogenicity, and stress response in the phytopathogenic fungus Alternaria alternata, providing genetic evidence for their central role in the biological functions of the fungus.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Miao Zhang, Yandong Zhang, Yongcai Li, Yang Bi, Renyan Mao, Yangyang Yang, Qianqian Jiang, Dov Prusky
Summary: This study systematically analyzed the effects of 30 mM H2O2 treatment on oxidative stress metabolism in Alternaria alternata, revealing the importance of cellular responses for oxidative stress tolerance in this necrotrophic fungus.
Article
Agronomy
Yangyang Yang, Pengdong Xie, Jing Yuan, Yongxiang Liu, Miao Zhang, Yongcai Li, Yang Bi, Dov B. Prusky
Summary: This study identifies the crucial role of AaCrz1 in the development, infection structure formation, calcium homeostasis, cell wall integrity, and virulence of the phytopathogenic fungus A. alternata.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Olumuyiwa Igbalajobi, Jia Gao, Reinhard Fischer
MOLECULAR PLANT-MICROBE INTERACTIONS
(2020)
Article
Biotechnology & Applied Microbiology
Cecilia Andreu, Javier Gomez-Peinado, Lex Winandy, Reinhard Fischer, Marcel li del Olmo
Summary: This study demonstrates the efficient expression of hydrophobins on the yeast cell surface using the Yeast Surface Display system, resulting in modified surface properties, increased resistance to adverse conditions, and enhanced biocatalytic activity. These results suggest potential applications for hydrophobin-exposing yeasts.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Michaela Duemig, Jasmin Binder, Anastasia Gaculenko, Franziska Daul, Lex Winandy, Mike Hasenberg, Matthias Gunzer, Reinhard Fischer, Markus Kuenzler, Sven Krappmann
Summary: Fungal spores play a crucial role in dispersal and survival, with specialized spores serving as infectious particles. The pathogenic fungus Aspergillus fumigatus produces conidia, which contain antimicrobial peptides that provide a growth advantage over bacterial competitors. This has implications for the ecological niche of A. fumigatus and its infectious process in causing aspergillosis.
CELLULAR MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Zhenzhong Yu, Jia Gao, Olumuyiwa Igbalajobi, Marek Skoneczny, Marzena Sienko, Agnieszka M. Maciejewska, Jerzy Brzywczy, Reinhard Fischer
Summary: The phytochrome-dependent light signaling pathway in fungi regulates the expression of genes related to stress responses and secondary metabolite production. The central regulator MetR plays a crucial role in controlling the expression of sulfur-assimilation genes and hundreds of other genes. Light and phytochrome also regulate the expression of carbon-sulfur hydrolases, providing insight into the interplay between light sensing and metabolic pathways.
Article
Biochemistry & Molecular Biology
Christian Streng, Jana Hartmann, Kai Leister, Norbert Krauss, Tilman Lamparter, Nicole Frankenberg-Dinkel, Franco Weth, Martin Bastmeyer, Zhenzhong Yu, Reinhard Fischer
Summary: This study reveals the involvement of mitochondria in light sensing in fungi, related to phytochrome dependence. Phytochrome photoreceptors in fungi contain a linear, heme-derived tetrapyrrole as chromophore, suggesting different approaches for chromophore biosynthesis and insertion into phytochrome in plants and fungi.
Article
Microbiology
Yifan Li, Tingting Sun, Degang Guo, Jia Gao, Jian Zhang, Feng Cai, Reinhard Fischer, Qirong Shen, Zhenzhong Yu
Summary: In Trichoderma guizhouense, the blue light receptor BLR1 dominates light responses while ENV1 is responsible for photoadaptation. The core components of the light signaling network, BLR1 and HOG1, control the expression of a large portion of the differentially expressed genes (DEGs). This study reveals a regulatory network based on blue light receptors and the MAPK HOG pathway for various processes in T. guizhouense, including conidiation and hydrophobin production.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Cell Biology
Xiaolei Gao, Saturnino Herrero, Valentin Wernet, Sylvia Erhardt, Oliver Valerius, Gerhard H. Braus, Reinhard Fischer
Summary: Centrosomes are essential microtubule-organizing centers in animal cells, while spindle pole bodies serve as functional analogs in fungi. Additional MTOCs have been identified in Aspergillus nidulans, with differing composition and organization from centrosomes. The polo-like kinase PlkA plays a crucial role in coordinating MTOC activities by interacting with gamma-tubulin ring complex receptors.
JOURNAL OF CELL SCIENCE
(2021)
Article
Microbiology
Valentin Wernet, Satur Herrero, Reinhard Fischer
Summary: The study reveals the differences in growth mechanisms between fast-growing fungi and slow-growing fungi, indicating a tradeoff between hyphal plasticity and growth speed.
Article
Multidisciplinary Sciences
Xi Yu, Xiaodi Hu, Maria Pop, Nicole Wernet, Frank Kirschhoefer, Gerald Brenner-Weiss, Julia Keller, Mirko Bunzel, Reinhard Fischer
Summary: The study revealed that the nematode-trapping fungus Duddingtonia flagrans utilizes 6-MSA to control trap formation and attract Caenorhabditis elegans. This compound plays a crucial role in interkingdom communication between fungi and nematodes.
NATURE COMMUNICATIONS
(2021)
Correction
Multidisciplinary Sciences
Xi Yu, Xiaodi Hu, Maria Pop, Nicole Wernet, Frank Kirschhofer, Gerald Brenner-Weiss, Julia Keller, Mirko Bunzel, Reinhard Fischer
NATURE COMMUNICATIONS
(2021)
Article
Genetics & Heredity
Zhenzhong Yu, Christian Streng, Ramon F. Seibeld, Olumuyiwa A. Igbalajobi, Kai Leister, Julian Ingelfinger, Reinhard Fischer
Summary: Fungi sense light of different wavelengths using blue-, green-, and red-light photoreceptors. While many genes in the model fungus Aspergillus nidulans are under light control, regulated through phytochrome and respond to specific wavelengths, there are also alternative red-light sensing systems and blue-light induced genes that may involve cryptochrome and phytochrome in the regulatory process. Phytochrome is essential for the response to blue light and plays a role in both blue- and red-light sensing pathways.
Article
Microbiology
Nicole Wernet, Valentin Wernet, Reinhard Fischer
Summary: Pathogenic microorganisms have different strategies to interact with their hosts, some modulate the host to obtain nutrients while others quickly kill and consume the host for energy. Predatory fungi produce a large number of small-secreted proteins to regulate the predation process, which is crucial for the predatory relationship between fungi and nematodes.
Article
Genetics & Heredity
Valentin Wernet, Jan Waeckerle, Reinhard Fischer
Summary: The STRIPAK complex plays a crucial role in filamentous fungi, controlling multicellular development, hyphal fusion, and pathogenicity. In the nematode-trapping fungus, a lack of STRIPAK component leads to incomplete trap formation, affecting its trapping function.
Article
Microbiology
Yifan Li, Xiya Meng, Degang Guo, Jia Gao, Qiwei Huang, Jian Zhang, Reinhard Fischer, Qirong Shen, Zhenzhong Yu
Summary: According to this study, blue light can increase conidial yield and stress resistance in Trichoderma. This is likely due to the upregulation of stress-related genes controlled by blue light receptors and MAP kinases.
Article
Multidisciplinary Sciences
Hamzeh Haj Hammadeh, Antonio Serrano, Valentin Wernet, Natascha Stomberg, Davina Hellmeier, Martin Weichert, Ulrike Brandt, Bianca Sieg, Konstantin Kanofsky, Reinhard Hehl, Reinhard Fischer, Andre Fleissner
Summary: This study identified a conserved cell dialogue mechanism in different fungal species, indicating the presence of a common molecular language that promotes cellular contact formation across species borders.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)