Article
Biotechnology & Applied Microbiology
Jianli Wang, Minghua Wu
Summary: Studies have shown that gastrodin upregulates miR-21 expression in HUVECs, leading to increased cell proliferation, migration, and tube formation ability, as well as the activation of the PI3K/Akt signaling pathway. This ultimately promotes angiogenesis in HUVECs.
Article
Acoustics
JiaYi Li, WeiMing Guo, Fei Yu, Lu Liu, XiaoTing Wang, LvYuan Li, Bing Fang, Lunguo Xia
Summary: The study demonstrated that LIPUS could promote the proliferation and angiogenic differentiation of endothelial cells (ECs) via the activation of the AKT signaling pathway. Additionally, LIPUS could alter the DNA methylation of ECs through the AKT signaling pathway activation, which in turn affected biomolecular changes in the cells.
Article
Medicine, Research & Experimental
Caixia Zhou, Yashu Kuang, Qinyu Li, Yunhao Duan, Xiuxiang Liu, Jinnan Yue, Xiaoli Chen, Jie Liu, Yuzhen Zhang, Lin Zhang
Summary: This study demonstrates that EC-expressing S1pr2 tightly controls post-ischemic angiogenesis and blood flow perfusion recovery. The inhibition of S1pr2 can enhance post-ischemic angiogenesis and improve blood flow in peripheral artery disease, offering a novel therapeutic intervention.
Article
Cell Biology
Jiyu Chen, Yanli Huang, Xiaochuan Hu, Xiaohong Bian, Sihui Nian
Summary: This study demonstrated that gastrodin protects against homocysteine-induced HUVEC injury by regulating the PI3K/Akt/eNOS and Nrf2/ARE pathways. Gastrodin reduced oxidative stress and increased cell viability, NO production, and vasorelaxation in thoracic aortic rings. The protective effects on NO production and vasorelaxation were mediated by PI3K/Akt/eNOS signaling and enhanced by Nrf2 activation, indicating the potential therapeutic benefits of gastrodin in vascular health.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Qingjie Mu, Kaixuan Lv, Jielun Yu, Shangmin Chu, Lichun Zhang, Lingyu Kong, Linlin Zhang, Yan Tian, Xiaopeng Jia, Benhong Liu, Youzhen Wei, Nana Yang
Summary: This study found that hydrogen can repair the proliferation, migration, and tube formation dysfunctions of lipopolysaccharide (LPS)-induced bone marrow-derived endothelial progenitor cells (EPCs). This repairment is mediated by the PI3K/AKT/eNOS signaling pathway.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Plant Sciences
Guo-Xia Guo, Ke-Yue Wu, Xiao-Yong Zhang, Fu-Xiang Lai, Karl Wah-Keung Tsim, Qi-Wei Qin, Wei-Hui Hu
Summary: This study investigated the effects of Curcumae Longae Rhizoma (CLR) extract on angiogenesis and found that it significantly suppressed angiogenic responses such as proliferation, migration, invasion, and tube formation of endothelial cells. It also reduced subintestinal vessel formation in a zebrafish embryo model. Mechanistically, CLR extract inhibited the VEGF-triggered signaling pathway, decreased phosphorylated VEGFR2 levels, and inhibited downstream regulators. It also reduced the production of NO and ROS. Additionally, CLR extract suppressed cell migration and NF-Kappa B translocation in cancer cells.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Chemistry, Medicinal
Jianpeng Chen, Zhikai Jiang, Xuao Liu, Kaitao Wang, Weijian Fan, Tingxiang Chen, Zhijie Li, Dingsheng Lin
Summary: The study found that berberine can improve the survival rate of random skin flaps by promoting angiogenesis, inhibiting inflammation, attenuating oxidative stress, and reducing apoptosis.
PHYTOTHERAPY RESEARCH
(2023)
Article
Plant Sciences
Fang Cao, Kai-Rong Qin, Kai Kang, Guoshuang Zheng, Weidan Wang, Xiuzhi Zhang, Dewei Zhao
Summary: Ginkgo biloba L. extract (EGb) has been widely used for its diverse pharmacological properties. This study aimed to investigate the effect and mechanism of EGb in preventing steroid-induced necrosis of the femoral head (SINFH).
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Food Science & Technology
Ho-Jeong Bang, Mok-Ryeon Ahn
Summary: This study found that pinobanksin exhibits antiangiogenic effect by inducing endothelial cell apoptosis, and it can be developed as an antiangiogenic agent. The mechanism involves inactivation of the Raf/MEK/ERK pathway and activation of apoptotic signaling such as caspase cascades.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Agriculture, Multidisciplinary
Ying Liu, Shumin Zhu, Jiaying Liu, Yonger Chen, Shaowen Zhong, Dawei Lian, Jian Liang, Song Huang, Shaozhen Hou
Summary: This study demonstrated that vitexin regulates angiogenesis and osteogenesis in ovariectomy-induced osteoporosis of rats via the VDR/eNOS signaling pathway, alleviating bone damage in the OVX rat model and promoting endothelial cell migration and tube formation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Cell & Tissue Engineering
Lingchi Kong, Yan Wang, Haixing Wang, Qi Pan, Rongtai Zuo, Shanshan Bai, Xiaoting Zhang, Wayne Yukwai Lee, Qinglin Kang, Gang Li
Summary: The study showed that microgravity enhances the pro-angiogenic properties of EPCs' paracrine signals through the HIF-1α/eNOS/NO axis, and the administration of MG-CM promotes bone fracture repair.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Yanan Lin, Yueying Jiang, Haiyu Xian, Xiaoxiao Cai, Tao Wang
Summary: In this research, the expression of the Pi3k/Akt pathway and VEGF in OSF were investigated, and their correlation was explored. It was found that the expression of Col-I increased as OSF progressed, but was downregulated in normal and moderate to advanced OSF tissues. VEGF expression correlated positively with Pi3k and Akt expression, and targeted regulation of the Pi3k/Akt pathway can induce VEGF expression and improve ischemia, ultimately treating OSF.
CELL PROLIFERATION
(2023)
Article
Biotechnology & Applied Microbiology
Wei Liu, Yu Feng, Xuehua Wang, Jiaxing Ding, Huili Li, Hongquan Guan, Zhijian Chen
Summary: Acute myocardial infarction (AMI) is a leading cause of human health issues worldwide. Exosome-based therapy, a cell-free approach, shows promise for repairing the ischemic myocardium. This study explores the effects and mechanism of human umbilical vein endothelial cells (HUVECs)-derived exosomes on AMI. The results demonstrate that HUVECs-derived exosomes can significantly improve cardiac function and inhibit cardiomyocyte apoptosis after AMI, possibly through the PI3K/AKT signaling pathway.
Article
Biochemistry & Molecular Biology
Kejia Kan, Yifei Mu, Marielle Bouschbacher, Carsten Sticht, Natalia Kuch, Martin Sigl, Nuh Rahbari, Norbert Gretz, Prama Pallavi, Michael Keese
Summary: The study revealed a novel biphasic response of human umbilical vein endothelial cells to blue light illumination. Low fluence blue light promoted cell activities, while high fluence illumination had the opposite effect by upregulating pro-apoptotic signaling pathways. These findings provide insights into photobiomodulation by blue light and potential treatment strategies for angiogenesis-dependent diseases.
Article
Pharmacology & Pharmacy
Wei Liu, Yanghui Ou, Yumeng Yang, Xuemei Zhang, Liqi Huang, Xiaohua Wang, Buling Wu, Mingcheng Huang
Summary: The study demonstrated that punicalagin has a protective effect on HUVECs activation through an endothelial-mediated mechanism, which may be used in the treatment of various disorders such as cancer, rheumatoid arthritis, and cardiovascular disease. This effect is achieved by reducing adhesion of monocyte cells to HUVECs, decreasing expression of ICAM-1 and VCAM-1, and inhibiting permeability, proliferation, migration, and tube formation in VEGF-induced HUVECs. Additionally, punicalagin suppresses IKK-mediated activation of NF-kappa B signaling and inhibits VEGFR2 activation and downstream p-PAK1.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Ke Wang, Yixuan Wang, Zebing Hu, Lijun Zhang, Gaozhi Li, Lei Dang, Yingjun Tan, Xinsheng Cao, Fei Shi, Shu Zhang, Ge Zhang
CELL DEATH & DISEASE
(2020)
Article
Cell Biology
Zhongyang Sun, Han Wang, Yuxiang Wang, Guodong Yuan, Xin Yu, Hui Jiang, Qi Wu, Binkui Yang, Zebing Hu, Fei Shi, Xinsheng Cao, Shu Zhang, Ting Guo, Jianning Zhao
Summary: The study identified the miR-103-3p/METTL14/m(6)A signaling axis as a critical regulator of osteoblast activity, with miR-103-3p targeting Mettl14 to inhibit osteoblast function and METTL14 promoting osteoblast activity through m(6)A methylation of miR-103-3p. Therapeutic inhibition of miR-103-3p reversed the decreased bone formation, indicating the potential of this signaling axis as a target for ameliorating osteoporosis.
Article
Multidisciplinary Sciences
Jingjing Dong, Honghui Wang, Gaozhi Li, Ke Wang, Yingjun Tan, Lijun Zhang, Yixuan Wang, Zebing Hu, Xinsheng Cao, Fei Shi, Shu Zhang
Summary: The combination of microgravity and radiation exacerbates the effects on MC3T3-E1 cells and rat femurs, leading to decreased cell activity, increased apoptosis rates, bone loss, and altered gene and protein expressions.
Article
Biochemistry & Molecular Biology
Honghui Wang, Jingjing Dong, Gaozhi Li, Yingjun Tan, Hai Zhao, Lijun Zhang, Yixuan Wang, Zebing Hu, Xinsheng Cao, Fei Shi, Shu Zhang
Summary: Experimental findings have shown that simulated microgravity or radiation can decrease MafG expression and increase apoptosis in MC3T3-E1 cells; meanwhile, increased ROS levels facilitate apoptosis in cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Physiology
Changyang Xing, Yuan Gao, Xinpei Wang, Wenjuan Xing, Yunnan Liu, Yujia Lei, Xing Zhang, Shu Zhang, Lijun Yuan, Feng Gao
Summary: Restricting blood flow in the thigh can effectively alleviate transient cerebral hypoperfusion induced by rapid shift from -Gz to + Gz, characterized by significant improvement in cerebral diastolic flow.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Liqun Xu, Lijun Zhang, Xiaoyan Zhang, Gaozhi Li, Yixuan Wang, Jingjing Dong, Honghui Wang, Zebing Hu, Xinsheng Cao, Shu Zhang, Fei Shi
Summary: The study demonstrated for the first time that mechanical unloading inhibits proliferation of brain microvascular endothelial cells through the HDAC6/miR-155-5p/RHEB axis, indicating that this pathway could be a specific target for preventative treatment of cardiovascular deconditioning.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Yuanyuan Yu, Luyao Wang, Shuaijian Ni, Dijie Li, Jin Liu, Hang Yin Chu, Ning Zhang, Meiheng Sun, Nanxi Li, Qing Ren, Zhenjian Zhuo, Chuanxin Zhong, Duoli Xie, Yongshu Li, Zong-Kang Zhang, Huarui Zhang, Mei Li, Zhenlin Zhang, Lin Chen, Xiaohua Pan, Weibo Xia, Shu Zhang, Aiping Lu, Bao-Ting Zhang, Ge Zhang
Summary: This study demonstrates that sclerostin has different mechanisms in protecting the cardiovascular system and inhibiting bone formation. By targeting sclerostin loop3, a drug can promote bone formation without affecting the cardiovascular system.
NATURE COMMUNICATIONS
(2022)
Article
Astronomy & Astrophysics
Liqun Xu, Xiaoyan Zhang, Gaozhi Li, Lijun Zhang, Shu Zhang, Fei Shi, Zebing Hu
Summary: In this study, it was found that miR-138-5p was upregulated under simulated microgravity, inhibiting osteoblast proliferation and promoting osteoblast apoptosis. Additionally, SIRT1 was downregulated and negatively correlated with miR-138-5p expression, suggesting that miR-138-5p inhibits osteoblast proliferation and promotes osteoblast apoptosis by targeting SIRT1. Therefore, the miR-138-5p/SIRT1 pathway should be considered for preventative treatment of disuse osteoporosis.
LIFE SCIENCES IN SPACE RESEARCH
(2023)
Article
Cell Biology
Lijun Zhang, Liqun Xu, Xiaoyan Zhang, Ke Wang, Yingjun Tan, Gaozhi Li, Yixuan Wang, Tong Xue, Quan Sun, Xinsheng Cao, Ge Zhang, Zebing Hu, Shu Zhang, Fei Shi
Summary: This study reveals for the first time the role of the Atf7ip/Setdb1/Macrod2 axis in osteoblast proliferation under mechanical unloading, providing a potential protective strategy against unloading-induced bone loss.
Article
Cell Biology
Lijun Zhang, Liqun Xu, Yixuan Wang, Xiaoyan Zhang, Tong Xue, Quan Sun, Hao Tang, Meng Li, Xinsheng Cao, Fei Shi, Ge Zhang, Shu Zhang, Zebing Hu
Summary: Emerging evidence suggests that multiple mechanisms are involved in bone loss induced by mechanical unloading. A new study shows that histone modification plays a crucial role in osteogenic differentiation under mechanical unloading. The study demonstrated that Setdb1, a histone methyltransferase, is increased during osteogenic differentiation and its knockdown blocks osteoblast function. Further investigation revealed that Setdb1 inhibits the expression of miR-212-3p, which suppresses osteogenic differentiation by downregulating Hmgb1 expression. These findings highlight the potential of the Setdb1/miR-212-3p/Hmgb1 pathway as a protective strategy against bone loss induced by mechanical unloading.
CELLULAR SIGNALLING
(2023)
Article
Genetics & Heredity
Xiaoyan Zhang, Tong Xue, Zebing Hu, Xian Guo, Gaozhi Li, Yixuan Wang, Lijun Zhang, Liqun Xu, Xinsheng Cao, Shu Zhang, Fei Shi, Ke Wang
Summary: This study identifies hub genes and explores novel molecular mechanisms underlying microgravity-induced bone loss, providing potential targets for countermeasures against this condition.
FRONTIERS IN GENETICS
(2022)
Article
Health Care Sciences & Services
Xiaoyan Zhang, Lijun Zhang, Liqun Xu, Gaozhi Li, Ke Wang, Tong Xue, Quan Sun, Hao Tang, Xinsheng Cao, Zebing Hu, Shu Zhang, Fei Shi
Summary: The secretion of exosomes with high expression of miR-92b-3p by microvascular endothelial cells (MVECs) under mechanical unloading conditions was found to inhibit the differentiation of MC3T3-E1 cells into mature osteoblasts. Further experiments showed that miR-92b-3p was transferred into MC3T3-E1 cells by exosomes, leading to the suppression of osteogenic differentiation. This discovery provides a potential strategy for studying and preventing mechanical unloading-induced bone loss and disuse osteoporosis.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Medicine, Research & Experimental
Luyao Wang, Yuanyuan Yu, Shuaijian Ni, Dijie Li, Jin Liu, Duoli Xie, Hang Yin Chu, Qing Ren, Chuanxin Zhong, Ning Zhang, Nanxi Li, Meiheng Sun, Zong-Kang Zhang, Zhenjian Zhuo, Huarui Zhang, Shu Zhang, Mei Li, Weibo Xia, Zhenlin Zhang, Lin Chen, Peng Shang, Xiaohua Pan, Aiping Lu, Bao-Ting Zhang, Ge Zhang
Summary: The sclerostin loop3-specific aptamer Apc001PE has been shown to promote bone formation without increasing cardiovascular risk in osteogenesis imperfecta (OI) mice, as granted orphan drug designation by US-FDA in 2019.
Review
Pharmacology & Pharmacy
Sifan Yu, Dijie Li, Ning Zhang, Shuaijian Ni, Meiheng Sun, Luyao Wang, Huan Xiao, Dingdong Liu, Jin Liu, Yuanyuan Yu, Zongkang Zhang, Samuel Tin Yui Yeung, Shu Zhang, Aiping Lu, Zhenlin Zhang, Baoting Zhang, Ge Zhang
Summary: Sclerostin, secreted by osteocytes, negatively regulates the WNT signaling pathway, inhibiting bone formation and promoting bone resorption. It is implicated in musculoskeletal diseases, cancers, obesity, and diabetes. Monoclonal antibodies, aptamers, and small-molecule inhibitors are inhibitors targeting sclerostin. The monoclonal antibody romosozumab, as the first-approved sclerostin inhibitor, effectively treats postmenopausal osteoporosis, but also poses cardiovascular risks.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Endocrinology & Metabolism
Yixuan Wang, Lijun Zhang, Ke Wang, Hua Zhou, Gaozhi Li, Liqun Xu, Zebing Hu, Xinsheng Cao, Fei Shi, Shu Zhang
Summary: This study observed reduced osteoblast number and bone formation in LPS-induced bone loss mice. The levels of circulating exosomes were increased and the expression of exosomal miRNAs was significantly changed in these mice. The increased exosomes inhibited the osteogenic differentiation of MC3T3-E1 cells.
CALCIFIED TISSUE INTERNATIONAL
(2022)