Article
Biochemistry & Molecular Biology
Raquel Leao Neves, Alyne Marem, Bruno Carmona, Julia Galanakis Arata, Marcos Paulo Cyrillo Ramos, Giselle Zenker Justo, Fabiana Henriques Machado de Melo, Vitor Oliveira, Marcelo Yudi Icimoto
Summary: The study found that oxidative stress influences the expression and regulation of THOP in leukemia cells, which may affect the immune evasion of tumor cells. Additionally, the proteolytic activity of THOP is also associated with the cellular redox state.
Article
Biochemistry & Molecular Biology
Edoardo Jun Mattioli, Jacopo Rossi, Maria Meloni, Marcello De Mia, Christophe H. Marchand, Andrea Tagliani, Silvia Fanti, Giuseppe Falini, Paolo Trost, Stephane D. Lemaire, Simona Fermani, Matteo Calvaresi, Mirko Zaffagnini
Summary: S-nitrosylation plays an important role in cellular signaling by modulating protein function and conformation. This study investigates the molecular mechanisms of GSNO-dependent thiol oxidation using CrGAPA. The research reveals that GSNO induces reversible enzyme inhibition through S-nitrosylation, particularly at the catalytic Cys149, leading to CrGAPA inactivation.
Article
Biochemistry & Molecular Biology
Sofia-Iris Bibli, Ingrid Fleming
Summary: This article discusses the impact of changes in oxidative balance on cellular physiology and adaptation, with a focus on the sensitivity of endothelial cells to reactive oxygen species and the role of oxidative modifications in regulating protein function. Persulfidation, a newly identified reversible oxidation modification, plays a crucial role in maintaining cellular redox homeostasis.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Biotechnology & Applied Microbiology
Nicolas Barraud, Sylvie Letoffe, Christophe Beloin, Joelle Vinh, Giovanni Chiappetta, Jean-Marc Ghigo
Summary: The study compared cysteine S-nitrosylation in aerobic and anaerobic biofilm and planktonic Escherichia coli cultures, identifying proteins with biofilm-specific S-nitrosylation status. Impairing S-nitrosylation in proteins related to redox homeostasis and amino acid synthesis altered important biofilm properties, such as motility, biofilm maturation, and resistance to oxidative stress, indicating the physiological basis of E. coli adaptation to the biofilm environment.
NPJ BIOFILMS AND MICROBIOMES
(2021)
Article
Biochemistry & Molecular Biology
Sudha Vijayan, Chitra Loganathan, Penislusshiyan Sakayanathan, Palvannan Thayumanavan
Summary: The study synthesized NOV-SAC and demonstrated its anti-proliferative activity against breast cancer cell line MCF-7 by inducing ROS generation and increasing the expression of p53, leading to apoptosis. The in vitro analysis results were consistent with the in silico findings.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Fisheries
Ajay Guru, Christy Lite, Allen J. Freddy, Praveen Kumar Issac, Mukesh Pasupuleti, N. T. Saraswathi, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Aziz Arshad, Jesu Arockiaraj
Summary: The study showed that WL15 peptide possesses strong antioxidant properties, which were validated through in vitro and in vivo experiments. Results from zebrafish experiments demonstrated that WL15 peptide can reduce intracellular ROS levels and inhibit apoptotic responses induced by H2O2.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
B. K. Sonu Koirala, Timothy Moural, Fang Zhu
Summary: This article provides a comprehensive overview of the versatile functions and diverse structures of insect GSTs in detoxifying chemical compounds. Recent advancements in technology have greatly enhanced our understanding of the functional and structural diversity of these enzymes.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Debojyoti Pal, Archita Rai, Rahul Checker, R. S. Patwardhan, Babita Singh, Deepak Sharma, Santosh K. Sandur
Summary: Mammalian cell survival and proliferation rely heavily on their response and adaptation to changes in the redox environment, with cancer cells often living in altered redox environments. Glutathione plays a crucial role in maintaining redox homeostasis by S-glutathionylation, protecting cysteine residues from oxidation and functioning as a sensor for redox status.Understanding the role of redox adaptations during carcinogenesis, including S-glutathionylation of specific redox-sensitive proteins, is important for developing new treatment strategies, such as redox targeting drugs, for refractory/resistant cancers.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Review
Biochemistry & Molecular Biology
Laura Troussicot, Bjorn M. Burmann, Mikael Molin
Summary: This review focuses on the structural studies and mechanisms underlying the chaperone function of 2-Cys PRDX systems, highlighting the coordination with other molecular chaperones. It also discusses the remarkable structural similarities between 2-Cys PRDXs, small HSPs, and J-domain-independent Hsp40 holdases in terms of their functions and dynamic equilibria.
Article
Biochemistry & Molecular Biology
Sudha Vijayan, Chitra Loganathan, Penislusshiyan Sakayanathan, Palvannan Thayumanavan
Summary: In recent years, derivatives of natural compounds have been synthesized to improve their bioavailability, pharmacology, and pharmacokinetics properties. This study successfully synthesized PLU-SAC ester, which showed no toxicity towards normal cells and exhibited anticancer activity by interacting with proteins involved in cancer therapy. In silico and in vitro analyses revealed the drug-like properties and mechanisms of PLU-SAC.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Immanuel Dhanasingh, Jae-Yoon Sung, Jae Won La, Eunju Kang, Dong-Woo Lee, Sung Haeng Lee
Summary: This study presents the crystal structure of FiPcp, which reveals the oxidized form of cysteine sulfonic acid in the catalytic triad. The molecular basis of redox-dependent catalysis of Pcp and the structural features related to thermostability are elucidated through this high-resolution structure analysis of the first inactive FiPcp with irreversible oxidative modification.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Zi-Hui Zhang, Jing-Hao Jin, Gui-Lin Sheng, Yu-Ping Xing, Wang Liu, Xue Zhou, Yi-Qing Liu, Xiao-Ren Chen
Summary: The SCR82 protein in Phytophthora pathogens plays dual roles as a virulence factor and a plant defense elicitor, showing conservation across Phytophthora species. SCR82 is quickly upregulated during infection initiation, triggering plant defense responses and impacting pathogen virulence and oxidative stress tolerance.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Psychiatry
Ana Maria Castejon, Jordan Ashley Spaw, Irina Rozenfeld, Nurit Sheinberg, Susan Kabot, Alexander Shaw, Patrick Hardigan, Rogerio Faillace, Edward E. Packer
Summary: This study found that a nutritional intervention with cysteine-rich whey protein isolate significantly increased glutathione levels and improved some behaviors associated with ASD in children. Further studies are needed to confirm these results.
FRONTIERS IN PSYCHIATRY
(2021)
Article
Biochemistry & Molecular Biology
Aaron P. Landry, Joseph Roman, Ruma Banerjee
Summary: This review discusses the enzymes involved in H2S homeostasis, cystathionine beta-synthase and sulfide quinone oxidoreductase, and their structural perspectives on reaction mechanisms and regulation. Understanding these key enzymes is crucial for understanding H2S homeostasis and its dysregulation in disease.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Yue Wang, Hui-Li Wang, Guang-Dong Xing, Yong Qian, Ji-Feng Zhong, Kun-Lin Chen
Summary: Heat stress is harmful to BMECs, and SAC can protect cells from oxidative damage and apoptosis by mediating the Nrf2/HO-1 signaling pathway.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Omar Pillaca-Pullo, David Rodrigues, Ignacio Sanchez-Moguel, Andre Lopes, Marcela Pimenta, Tajindar Basi, Valker Feitosa, Amparo Iris Zavaleta, Gisele Monteiro, Adalberto Pessoa Jr, Michele Vitolo
Summary: The study showed that under specific conditions, a biomass of around 53 g L(-1) and ASNase volumetric activity of 710 U L(-1) can be achieved using buffered glycerol-complex medium (BMGY) containing 40 g L(-1) glycerol, with induction after 141 hours using 3.0% (v/v) methanol, at 20 degrees Celsius and initial pH 6.0. These results indicate the scalability of the experiments from shake flasks to a 3L-bioreactor, suggesting potential for industrial production.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marilene Demasi, Ohara Augusto, Etelvino J. H. Bechara, Renata N. Bicev, Fernanda M. Cerqueira, Fernanda M. da Cunha, Ana Denicola, Fernando Gomes, Sayuri Miyamoto, Luis E. S. Netto, Lia M. Randall, Cassius V. Stevani, Leonor Thomson
Summary: Research on protein oxidation began in 1980, exploring the impact of oxidative modifications on protein function and cellular homeostasis. Recent studies have shown that not all oxidative modifications result in damage, but can also alter protein structure and function. Understanding the effects of protein oxidation on human pathological conditions is clinically significant.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Biotechnology & Applied Microbiology
Leticia de Almeida Parizotto, Eduardo Krebs Kleingesinds, Luiza Manfrinato Pedrotti da Rosa, Brian Effer, Guilherme Meira Lima, Marcos Edgar Herkenhoff, Zhaopeng Li, Ursula Rinas, Gisele Monteiro, Adalberto Pessoa, Aldo Tonso
Summary: This study focused on the production of glycosylated L-asparaginase of Dickeya chrysanthemi expressed by Pichia pastoris Glycoswitch (R). By comparing producer strains with histidine auxotrophy and prototrophic strains, it was found that the prototrophic strain showed higher productivity in both scales. Two induction strategies were executed with the prototrophic strain, methanol pulses and a DO-stat, resulting in the latter having 2-fold more maximum volumetric activity. Additionally, glycosylation analysis showed reduced potential immunogenic effects by glycosylating the site associated with allergies in patients.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Microbiology
Juan Carlos Flores-Santos, Ignacio S. Moguel, Gisele Monteiro, Adalberto Pessoa, Michele Vitolo
Summary: In this study, the production of ASNase in E. coli was optimized by modifying the culture medium with glycine and n-dodecane, resulting in significant increases in enzyme yield and extracellular enzymatic productivity. This proposed approach presents a simple and effective strategy to enhance extracellular production of ASNase in E. coli.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Guilherme M. Lima, Alexey Atrazhev, Susmita Sarkar, Mirat Sojitra, Revathi Reddy, Karin Torres-Obreque, Carlota de Oliveira Rangel-Yagui, Matthew S. Macauley, Gisele Monteiro, Ratmir Derda
Summary: This study demonstrates a method for displaying large multisubunit proteins on phages by connecting different fusion proteins to phage clones displaying peptides. The research findings suggest that different valencies and chemical modifications can affect the clearance rate of proteins in vivo.
ACS CHEMICAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Iris Munhoz Costa, Debora Custodio Moura, Guilherme Meira Lima, Adalberto Pessoa, Camila Oresco dos Santos, Marcos A. de Oliveira, Gisele Monteiro
Summary: The study developed a double-mutant ASNase with higher specific activity through directed evolution, reducing the necessary protein amount for treatment and lowering toxicity. In vitro experiments showed reduced antibody recognition and similar cytotoxicity with 40% less protein mass, potentially improving anti-ALL therapy for acute lymphoblastic leukemia patients.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Carolina A. Parada, Ivan Pires de Oliveira, Mayara C. F. Gewehr, Joao Agostinho Machado-Neto, Keli Lima, Rosangela A. S. Eichler, Lucia R. Lopes, Luiz R. G. Bechara, Julio C. B. Ferreira, William T. Festuccia, Luciano Censoni, Ivarne Luis S. Tersariol, Emer S. Ferro
Summary: This study investigates the intracellular effects of intracellular peptides on protein-protein interactions. The results show that VFD7 and VFD6 peptides can inhibit the rapamycin-induced interaction between FKBP and FRB. Molecular dynamics simulations suggest that VFD7 and VFD6 can bind to FKBP and induce structural changes, providing insights into their mechanism of PPI inhibition. Additionally, intracellular peptides are found to be mainly associated with macromolecular components. Overall, this study provides further evidence for the importance of intracellular peptides in the targeted design of therapeutic molecules for intracellular PPI.
Article
Biochemistry & Molecular Biology
Renata Silverio, Robson Barth, Andrea S. Heimann, Patricia Reckziegel, Gustavo J. dos Santos, Silvana Y. Romero-Zerbo, Francisco J. Bermudez-Silva, Alex Rafacho, Emer S. Ferro
Summary: Oral administration of Pep19 can alleviate high-fat diet-induced obesity and have positive effects on metabolic traits, morphology, and behavior.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Henrique P. Biasoto, Cristina B. Hebeda, Sandra H. P. Farsky, Adalberto Pessoa, Tales A. Costa-Silva, Gisele Monteiro
Summary: L-asparaginase (ASNase) is an effective inhibitor of tumor development and is used in chemotherapy for acute lymphoblastic leukemia. L-asparaginase II from Saccharomyces cerevisiae shows potential to replace bacterial ASNase for patients with hypersensitivity. In this study, we describe a genetic process to produce extracellular ScASNaseII, which exhibits higher stability and lower cross-reaction with antibodies compared to commercially available ASNase.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Marina de Souza Guimaraes, Jorge Javier Muso Cachumba, Cecilia Zorzi Bueno, Karin Mariana Torres-Obreque, Grace Veronica Ruiz Lara, Gisele Monteiro, Leandro Ramos Souza Barbosa, Adalberto Pessoa Jr, Carlota de Oliveira Rangel-Yagui
Summary: L-asparaginase is an important biological drug used for the treatment of Acute Lymphoblastic Leukemia. Encapsulation of L-asparaginase in liposomes provides protection against degradation and improves the pharmacokinetics of the drug. The study showed that the encapsulated L-asparaginase exhibited enhanced activity and improved protection against immune recognition and protease degradation in vitro and in vivo.
Article
Chemistry, Medicinal
Maria Eduarda Mazzi Esquinca, Claudia Neves Correa, Gabriel Marques de Barros, Horacio Montenegro, Leandro Mantovani de Castro
Summary: Sea anemones, sessile invertebrates of the phylum Cnidaria, have the ability to produce and inoculate venom, making it crucial for their survival and evolution. This study used a multi-omics approach to analyze the protein composition of the tentacles and mucus of Bunodosoma caissarum, a species of sea anemone found in Brazil. The results revealed a large number of annotated genes in the tentacles, with 1% showing similarity to toxins or toxin-related proteins. Proteome analysis identified abundant enzymes in the tentacles and toxins in the mucus. The peptidomics analysis further identified various fragments of toxins and peptides. Overall, this study provides valuable insights into the composition of sea anemone tentacles and mucus, including the identification of previously unknown genes and potential therapeutic toxin-like proteins.
Article
Biochemistry & Molecular Biology
Claudia Neves Correa, Louise Oliveira Fiametti, Gabriel Marques de Barros, Leandro Mantovani de Castro
Summary: This study identified 1080 peptides in the gills of the seahorse H. reidi using fast heat inactivation of proteases and liquid chromatography coupled to mass spectrometry. Among these peptides, 1013 were present in all samples and 67 were identified in an additional LC-MS/MS run from an alkylated and reduced pool of samples. The majority of the identified peptides were fragments of the internal region of precursor proteins, including ribosomal proteins, histones, and hemoglobin, and some fragments from moronecidin-like protein with antimicrobial activity were also found.
Article
Biochemistry & Molecular Biology
Iris Munhoz Costa, Brian Effer, Tales Alexandre Costa-Silva, Chen Chen, Michael F. Ciccone, Adalberto Pessoa, Camila O. dos Santos, Gisele Monteiro
Summary: L-Asparaginase (ASNase) is an important drug for the treatment of acute lymphoblastic leukemia (ALL), but some ALL cases are naturally resistant to ASNase treatment. The REH ALL cell line, which is resistant to ASNase treatment, was used to investigate the role of cathepsin B (CTSB) in ASNase resistance. The deletion of CTSB in REH ALL cells did not confer sensitivity to ASNase treatment, suggesting that intrinsic expression of CTSB is not a mechanism driving resistance in these ALL cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Lucas G. G. Viviani, Daria B. B. Kokh, Rebecca C. C. Wade, Antonia T. do Amaral
Summary: Human ecto-5'-nucleotidase (h-ecto-5'-NT, CD73) is an important enzyme involved in purinergic signaling pathways and considered a potential therapeutic target for various diseases. However, the dynamics of h-ecto-5'-NT are poorly understood, posing challenges for inhibitor design. In this study, molecular dynamics simulations revealed significant differences between the open and closed conformations of h-ecto-5'-NT and showed that a nucleotide analogue inhibitor could stabilize the closed conformation. The substrate binding site of h-ecto-5'-NT was explored, and transient subpockets near the binding site were identified. The study provides insights into the structural flexibility of h-ecto-5'-NT and can guide the design of novel inhibitors.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Biochemistry & Molecular Biology
Eduardo Krebs Kleingesinds, Leticia de Almeida Parizotto, Brian Effer, Gisele Monteiro, Paul F. Long, Yoana Arroyo-Berdugo, Volker Behrends, Maria Teresa Esposito, Yolanda Calle, Adalberto Pessoa-Jr
Summary: This study presents the downstream process of L-asparaginase from Erwinia chrysanthemi expressed by Pichia pastoris with human-like glycosylation pattern and its impact on leukemic cells co-cultured with bone marrow cytoprotective stromal cells. The researchers obtained an extremely pure enzyme with a yield of 54.93%. Glycosylated L-ASNase showed complete inhibition of proliferation in tested leukemia cell lines, independently of the presence of BM stromal cells. However, the cytotoxic efficacy of glycosylated L-ASNase varied across cell lines, with ALL cell lines being the most sensitive, and was partially inhibited by BM stromal cells.
PROCESS BIOCHEMISTRY
(2023)