Article
Immunology
Weipeng Wang, Ying Wang, Ying Lu, Jing Zhu, Xinbei Tian, Bo Wu, Jun Du, Wei Cai, Yongtao Xiao
Summary: Regenerating islet-derived family member 4 (Reg4) has been found to possess potent bactericidal activity against Salmonella Typhimurium (S. Tm), inhibiting colonization of S. Tm in the intestine and ameliorating intestinal inflammation. Reg4 proteins can restrict Salmonella invasion of intestinal epithelia by binding to the flagella of Salmonella, suppressing bacterial motility and the host inflammatory response.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Infectious Diseases
Wael A. H. Hegazy, Ibrahim M. Salem, Hadil Faris Alotaibi, El-Sayed Khafagy, Doaa Ibrahim
Summary: The study finds that the alpha-adrenergic antagonist terazosin has an anti-virulence effect on S. Typhimurium. Terazosin reduces the adhesion, biofilm formation, invasion, and intracellular replication of S. Typhimurium. It also interferes with quorum sensing and bacterial espionage, as well as down-regulates the expression of the type three secretion system (T3SS) encoding genes.
Review
Microbiology
Xiaojie Zhang, Baobao Liu, Xueyan Ding, Peng Bin, Yang Yang, Guoqiang Zhu
Summary: The QS system plays a central role in the regulation of virulence in Salmonella, and blocking this system may be a new strategy for treating Salmonella infection. Studying the application of QQ technology in Salmonella infection can provide insight into the regulation mechanism of bacterial virulence.
Article
Biochemistry & Molecular Biology
Wanwu Li, Linxing Li, Xiaolin Yan, Pan Wu, Tianli Zhang, Yu Fan, Shuai Ma, Xinyue Wang, Lingyan Jiang
Summary: The utilization of nitrate promotes Salmonella Typhimurium systemic infection and virulence by contributing to cytoplasm acidification and transcription of virulence genes, and this advantage occurs only under low-oxygen conditions and is regulated by Fnr and the NarX-NarL two-component system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Shamsun Nahar, Ha Lim Jeong, Younsoo Kim, Angela Jie-won Ha, Pantu Kumar Roy, Si Hong Park, Md Ashrafudoulla, Md Furkanur Rahaman Mizan, Sang-Do Ha
Summary: The inhibitory properties of Flavourzyme against Salmonella enterica and Shiga toxin-producing Escherichia coli were evaluated, showing significant inhibition of biofilm formation and quorum sensing activity, along with suppression of gene expression related to biofilm formation, quorum sensing, and virulence.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Sherif G. Ahmed, Giulia Oliva, Manlin Shao, Xinhui Wang, John J. Mekalanos, Gary J. Brenner
Summary: This study evaluated the efficacy of Salmonella typhimurium as an immunotherapy for schwannomas. It was found that Salmonella typhimurium can control tumor growth and induce host immune responses. Intratumoral injection of Salmonella typhimurium led to tumor cell apoptosis and inhibited tumor angiogenesis. Additionally, Salmonella typhimurium treatment induced systemic antitumor immunity and reduced the growth of rechallenge tumors. Combination therapy of Salmonella typhimurium and PD-1 blockade showed enhanced suppression of schwannoma growth.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Microbiology
Barbara M. Schultz, Felipe Melo-Gonzalez, Geraldyne A. Salazar, Barbara N. Porto, Claudia A. Riedel, Alexis M. Kalergis, Susan M. Bueno
Summary: Salmonella enterica is a common source of food and water-borne infections, causing a wide range of clinical ailments in both human and animal hosts. Immunity to Salmonella involves an interplay between different immune responses, despite the bacterium's strategies to evade host immune responses.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Joanna Plawinska-Czarnak, Karolina Wodz, Magdalena Guzowska, Elzbieta Rosiak, Tomasz Nowak, Zuzanna Strzalkowska, Adam Kwiecinski, Piotr Kwiecinski, Krzysztof Anusz
Summary: In this study, Salmonella BO strain was isolated from human milk and analyzed using whole-genome sequencing, serosequencing, and genotyping. The results revealed that the strain belongs to Salmonella enterica subsp. enterica serovar Typhimurium 4:i:1,2_69M (S. Typhimurium 69M) and showed significant changes in gene sequences. The presence of antimicrobial resistance genes, although not directly implying antibiotic resistance phenotype, was also detected.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Abrar K. Thabit, Khalid Eljaaly, Ayat Zawawi, Tarek S. Ibrahim, Ahmed G. Eissa, Samar S. Elbaramawi, Wael A. H. Hegazy, Mahmoud A. Elfaky
Summary: The study found that atenolol can effectively reduce the biofilm formation, invasion, and replication of Salmonella Typhimurium. Atenolol affects the virulence of Salmonella by downregulating related genes and protects mice from infection. In silico molecular docking study showed that atenolol can compete with the target proteins of Salmonella.
Article
Biochemistry & Molecular Biology
Troy Burtchett, Chloe Love, Reshma Sarkar, Brian C. Tripp
Summary: The study identified the crucial role of four channel-lining C-terminal residues in facilitating flagellin transport and determining flagellar stability and morphology. Substitution of these residues with alanine led to decreased motility and flagellar assembly, impacting flagellar function and structure.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Microbiology
Cong Li, Gregory H. Tyson, Chih-Hao Hsu, Lucas Harrison, Errol Strain, Thu-Thuy Tran, Glenn E. Tillman, Uday Dessai, Patrick F. McDermott, Shaohua Zhao
Summary: This study used long-read sequencing to investigate the genomic heterogeneity and antimicrobial resistance mechanisms of Salmonella isolated from retail meats and food animals. It found significant genetic variation among different serotypes, with the presence of plasmids playing a key role in genomic variability. Moreover, the bacteria's resistance mechanisms may be associated with plasmid types, sources, and serotypes.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Yuanyuan Wang, Chunmei Wu, Jiacong Gao, Xudong Du, Xiangyun Chen, Mei Zhang
Summary: Salmonella enterica serovar Typhimurium triggers reprogramming of host cell metabolism and an inflammatory response during systemic infection, promoting its survival and replication. Additionally, infection by Salmonella may induce M1 and M2 polarization in macrophages, and alter the actin cytoskeleton to facilitate the formation of Salmonella-containing vacuoles.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Biochemistry & Molecular Biology
Mariana de Fatima Albuquerque Pereira, Larissa Gabriela Morais de Avila, Bruna Cristina dos Santos Cruz, Bruno Campos Silva, Leandro Licursi de Oliveira, Reggiani Vilela Goncalves, Andrea de Oliveira Barros Ribon, Tiago Antonio de Oliveira Mendes, Maria do Carmo Gouveia Peluzio
Summary: This study evaluated the role of milk kefir on inflammation and gut microbiome in mice infected with Salmonella. The results showed that kefir could prevent systemic infections and regulate cytokines in IL-10(-/-) mice, but not in wild type mice. These findings demonstrate the importance of IL-10 in the prognosis of salmonellosis and the potential use of milk kefir in acute infections.
Article
Biochemistry & Molecular Biology
German Pozdeev, Aalap Mogre, Charles J. Dorman
Summary: DNA gyrase, composed of GyrA and GyrB subunits, plays a crucial role in bacterial physiology. The positioning of gyrase genes can affect various functions within the bacteria, with a particular impact on virulence genes like SPI-2. Understanding the significance of gene arrangements in bacteria can provide insights into genome evolution and the sensitivity of certain genes to disruptions in DNA topology.
MOLECULAR MICROBIOLOGY
(2021)
Article
Food Science & Technology
Zixuan Xu, Zhenyang Hu, Zhilong Yu, Lijun Huang, Fangwei Yang, Yunfei Xie
Summary: Virtual screening targeting LuxS and LsrB proteins of Salmonella was used to identify 12 quorum sensing inhibitors (QSIs) applicable in the food industry. Among them, esculetin showed the lowest MIC and demonstrated significant inhibitory effects on Salmonella's motility, biofilm formation, extracellular polymer, and AI-2 signaling molecule production. Esculetin down-regulated the expression of genes related to quorum sensing and biofilm formation. This study highlights the potential of virtual screening and esculetin as a preservative in the food industry.
Article
Multidisciplinary Sciences
Mauricio H. Pontes, Eun-Jin Lee, Jeongjoon Choi, Eduardo A. Groisman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Biochemistry & Molecular Biology
Jeongjoon Choi, Eduardo A. Groisman
MOLECULAR MICROBIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Jeongjoon Choi, Eduardo A. Groisman
Article
Biochemistry & Molecular Biology
Jeongjoon Choi, Eduardo A. Groisman
Article
Biochemistry & Molecular Biology
Jinki Yeom, Mauricio H. Pontes, Jeongjoon Choi, Eduardo A. Groisman
Article
Microbiology
Eunna Choi, Daesil Nam, Jeongjoon Choi, Shinae Park, Jung-Shin Lee, Eun-Jin Lee
Article
Biotechnology & Applied Microbiology
Jeongjoon Choi, Sangryeol Ryu
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Microbiology
Jeongjoon Choi, Heeju Kim, Yoonjee Chang, Woongjae Yoo, Dajeong Kim, Sangryeol Ryu
Article
Multidisciplinary Sciences
Jeongjoon Choi, Eduardo A. Groisman
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Jeongjoon Choi, Eduardo A. Groisman
NUCLEIC ACIDS RESEARCH
(2020)
Article
Chemistry, Medicinal
Woongjae Yoo, Jeongjoon Choi, Bookyung Park, Mariana X. Byndloss, Sangryeol Ryu
Summary: This study demonstrates that Salmonella utilizes 1,2-propanediol through EIIA(Ntr)-mediated regulation of the pdu operon to expand in the murine intestine. The metabolic intermediate, Propionyl-CoA, produced during 1,2-propanediol catabolism, elevates EIIA(Ntr) protein amounts and enhances the utilization process. This activation by EIIA(Ntr) may be conserved in other pathogenic enterobacteria like Escherichia coli, suggesting a common mechanism for utilizing microbiota-derived nutrients.
ACS INFECTIOUS DISEASES
(2021)
Review
Microbiology
Eduardo A. Groisman, Alexandre Duprey, Jeongjoon Choi
Summary: The PhoP/PhoQ two-component system is crucial in governing virulence, Mg2+ homeostasis, and resistance to antimicrobial agents in Gram-negative bacteria. The system involves the sensor PhoQ and the transcriptional regulator PhoP, with PhoQ being activated by various signals to promote the phosphorylated state of PhoP. This phosphorylated form controls the expression of hundreds of proteins directly and indirectly, revealing novel forms of signal transduction and the physiological consequences of regulon evolution.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2021)
Article
Multidisciplinary Sciences
Hyuk-Kwon Kwon, Inkyu Lee, Kristin E. Yu, Sean Cahill, Kareme D. Alder, Saelim Lee, Christopher M. Dussik, JungHo Back, Jeongjoon Choi, Lee Song, Themis R. Kyriakides, Francis Y. Lee
Summary: The text discusses the challenge of treating joint infections and presents a new treatment modality involving locally delivered cell-penetrating antibiotics to eradicate bacteria and targeting inflammation to protect articular cartilage, offering a promising new therapeutic strategy for septic arthritis.
Article
Biochemistry & Molecular Biology
Jeongjoon Choi, Hubert Salvail, Eduardo A. Groisman
Summary: The study reveals that in Salmonella enterica serovar Typhimurium, the RNA chaperone CspC is essential for activating the master virulence regulator PhoP in mildly acidic pH conditions, while CspE is not necessary. This activation is mediated by the virulence gene ugtL and enhances translation by disrupting mRNA secondary structure.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Genetics & Heredity
Hubert Salvail, Jeongjoon Choi, Eduardo A. Groisman
Summary: Operon organization can lead to the concerted transcription of genes, with some operons producing two mRNAs but only allowing translation of certain genes. In the case of a bicistronic operon involving the novel gene ugtS and the virulence gene ugtL in Salmonella enterica serovar Typhimurium, only the longer mRNA permits translation of both genes. The small protein UgtS plays a role in regulating the virulence program by controlling the phosphorylation kinetics of the master virulence regulator PhoP.