Article
Oncology
Shouhei Miyagi, Takahiro Watanabe, Yuya Hara, Masataka Arata, Md. Kamal Uddin, Keisuke Mantoku, Ken Sago, Yusuke Yanagi, Takeshi Suzuki, H. M. Abdullah Al Masud, Jun-ichi Kawada, Shigeo Nakamura, Yasuyuki Miyake, Yoshitaka Sato, Takayuki Murata, Hiroshi Kimura
Summary: The STING inhibitor C-176 suppresses EBV-induced transformation in peripheral blood mononuclear cells, prolongs survival, and reduces tumor formation. Even without direct contact between B cells and T cells, the STING inhibitor can lower the transformation activity of EBV through intercellular communication.
Review
Immunology
Patrick Schuhmachers, Christian Munz
Summary: EBV is a highly successful pathogen in humans, infecting over 95% of the adult population, but the immune system is able to control it in most carriers. It is primarily found in lymphomas and epithelial cell carcinomas, with the emergence of EBV-positive tumors requiring additional factors such as co-infections or genetic predispositions. Research in humanized mice provides a valuable platform for testing therapies and vaccines against EBV-associated pathologies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Jie Xiong, Yu-Ting Dai, Wen-Fang Wang, Hao Zhang, Chao-Fu Wang, Tong Yin, Shu Cheng, Hui -Juan Zhong, Shan-He Yu, Lu Jiang, Sheng-Yue Wang, Hai Fang, Rui-Hong Zhang, Yue Zhu, Hong-Mei Yi, Xu-Feng Jiang, Jia-Yi Chen, Li Wang, Peng -Peng Xu, Sai -Juan Chen, Wei-Li Zhao
Summary: This study provides a comprehensive characterization of natural killer/T-cell lymphoma (NKTCL) at the proteogenomic level and reveals the correlation between EBV gene patterns and oncogenic signaling. Single-cell transcriptome analysis further uncovers the association between immune phenotypes and the cancer-immunity cycle. The study also identifies a GPCR-mediated mechanism of malignant progression.
Article
Multidisciplinary Sciences
Xueyi Zheng, Ruixuan Wang, Xinke Zhang, Yan Sun, Haohuan Zhang, Zihan Zhao, Yuanhang Zheng, Jing Luo, Jiangyu Zhang, Hongmei Wu, Dan Huang, Wenbiao Zhu, Jianning Chen, Qinghua Cao, Hong Zeng, Rongzhen Luo, Peng Li, Lilong Lan, Jingping Yun, Dan Xie, Wei-Shi Zheng, Junhang Luo, Muyan Cai
Summary: This study introduces a deep convolutional neural network called EBVNet and its fusion with pathologists for predicting Epstein-Barr virus-associated gastric cancer (EBVaGC) from histopathology. EBVNet shows high accuracy in discriminating EBVaGC and the human-machine fusion significantly improves diagnostic performance.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Jakob Woerner, Yidi Huang, Stephan Hutter, Carmelo Gurnari, Jesus Maria Hernandez Sanchez, Janet Wang, Yimin Huang, Daniel Schnabel, Michael Aaby, Wanying Xu, Vedant Thorat, Dongxu Jiang, Babal K. Jha, Mehmet Koyuturk, Jaroslaw P. Maciejewski, Torsten Haferlach, Thomas LaFramboise
Summary: This study analyzed deep DNA sequence data from 1870 patients with myeloid malignancies and found evidence of dysbiosis in disease cases, as well as distinct microbial signatures among disease subtypes. It also highlighted the association between microbial content and host gene mutations and myeloblast cell percentages. The study provided evidence that Epstein-Barr virus status refines risk stratification in low-risk myelodysplastic syndrome patients and constructed machine-learning classifiers to discriminate among disease subtypes based solely on bacterial content.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Xiao Zhang, Junping Hong, Ling Zhong, Qian Wu, Shanshan Zhang, Qianying Zhu, Haiwen Chen, Dongmei Wei, Rui Li, Wanlin Zhang, Xinyu Zhang, Guosong Wang, Xiang Zhou, Junyu Chen, Yinfeng Kang, Zhenghui Zha, Xiaobing Duan, Yang Huang, Cong Sun, Xiangwei Kong, Yan Zhou, Yanhong Chen, Xiaoping Ye, Qisheng Feng, Shaowei Li, Tong Xiang, Song Gao, Mu-Sheng Zeng, Qingbing Zheng, Yixin Chen, Yi-Xin Zeng, Ningshao Xia, Miao Xu
Summary: This study identified two neutralizing antibodies that effectively neutralize EBV infection and provide protection against EBV-induced lymphoproliferative disorders in humanized mice. The antibodies inhibit membrane fusion through interference with gB-cell interaction and activation, offering potential targets for antiviral therapies and vaccines.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Yoshimi Enose-Akahata, Limin Wang, Fahad Almsned, Kory R. Johnson, Yair Mina, Joan Ohayon, Xin Wei Wang, Steven Jacobson
Summary: Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system (CNS), and the cause of MS remains unknown. This study investigated the antibody responses against various viruses in the cerebrospinal fluid (CSF) and serum of MS patients, and found significant differences compared to healthy volunteers, as well as a pattern of antibody responses against multiple viruses, including Epstein-Barr virus. These findings indicate that virus-specific antibody signatures might reflect the disease-associated inflammatory milieu in the CSF of patients with neuroinflammatory diseases.
Article
Biochemistry & Molecular Biology
Yumie Takagi, Katsuko Sudo, Sachiko Yamaguchi, Shuzo Urata, Tatsukuni Ohno, Sachiko Hirose, Kiyoshi Matsumoto, Takashi Kuramoto, Tadao Serikawa, Jiro Yasuda, Masashi Ikutani, Susumu Nakae
Summary: Severely immunodeficient mice are useful for studying tumor pathogenesis and developing therapeutic agents. Engrafting these mice with human hematopoietic cells helps understand in vivo molecular mechanisms of viral infections. A novel strain of severely immunodeficient mice with a mutation in the Prkdc gene exon 57 was discovered, which showed susceptibility to influenza viruses.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Oncology
Laura E. Martinez, Tracy R. Daniels-Wells, Yu Guo, Larry Magpantay, Pierre Candelaria, Manuel L. Penichet, Otoniel Martinez-Maza, Marta Epeldegui
Summary: The anti-TfR1 antibody, ch128.1/IgG1, effectively inhibits the activation, growth, and immortalization of EBV+ human B-cells in vivo, as well as the development of these cells into lymphoma-like tumors in immunodeficient mice.
MOLECULAR CANCER THERAPEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Masatoshi Ito, Kai Kudo, Hiroshi Higuchi, Hiroko Otsuka, Masayuki Tanaka, Nahoko Fukunishi, Takuma Araki, Masako Takamatsu, Yoko Ino, Yayoi Kimura, Ai Kotani
Summary: The study revealed that EVs derived from EBV-transformed lymphomas contain immunomodulatory proteins and phospholipids associated with TAM formation, which may contribute to the development of lymphomas.
Article
Immunology
Joanna Musialik, Aureliusz Kolonko, Andrzej Wiecek
Summary: The study aimed to assess the impact of SARS-CoV-2 vaccines on EBV infections in stable kidney and liver transplant recipients. The results showed a significant increase in EBV viremia after the second vaccine dose in 9 out of 10 patients, and one patient experienced reactivation of EBV infection. Although the viral load decreased six months later, some patients still had a viral load higher than the baseline.
Article
Pathology
Sudipta Samanta, Muthukaruppan Swaminathan, Jianing Hu, Khai Tuck Lee, Ajitha Sundaresan, Chuan Keng Goh, Chor Hiang Siow, Kwok Seng Loh, Soh Ha Chan, Joshua K. Tay, Ian Cheong
Summary: The use of immunofluorescence assay for detecting serum Epstein-Barr virus antibodies is the gold standard screening test for nasopharyngeal cancer in high-risk populations. This study demonstrates that integrating deep learning with automated fuzzy inference can improve the scalability and accuracy of NPC detection.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Environmental Sciences
Mengmeng Li, Wen-Jie Chen, Jun Yang, Hadrien Charvat, Shang-Hang Xie, Tong Li, Wei Ling, Yu-Qiang Lu, Qing Liu, Ming-Huang Hong, Su-Mei Cao
Summary: This study investigated the association between solid fuel use and Epstein-Barr virus (EBV) seropositivity, as well as EBV activation related to nasopharyngeal carcinoma (NPC). The results showed that solid fuel use was associated with a higher risk of EBV seropositivity and NPC-related EBV activation. The findings have important implications for understanding the etiology of NPC and public health, particularly in low resource settings.
ENVIRONMENTAL POLLUTION
(2022)
Article
Hematology
Hannes Vietzen, Philippe L. Furlano, Jan J. Cornelissen, Georg A. Boehmig, Peter Jaksch, Elisabeth Puchhammer-Stoeckl
Summary: This study found that nonclassic human leukocyte antigen E (HLA-E)-restricted immune responses have a significant impact on the development of Epstein-Barr virus (EBV) diseases in the individual host. The highly expressed HLA-E*0103/0103 genotype is protective against infectious mononucleosis (IM) by inducing potent EBV BZLF1-specific HLA-E-restricted CD8(+) T-cell responses. Variations in the inhibitory NKG2A/LMP-1/HLA-E axis are associated with the risk of symptomatic EBV reactivations in both immunocompetent individuals and immunocompromised transplant recipients.
Article
Multidisciplinary Sciences
Hyun Ju Park, Eun Jeong Cho, Ji-Hun Kim, Sehun Lim, Chang Ohk Sung
Summary: This study identifies the expression of Epstein-Barr virus (EBV) in the tumor immune microenvironment (TIM) of colorectal cancer (CRC) tissue. EBV was detected in tumor-infiltrating lymphocytes, and its positivity was associated with older age, male sex, and SMAD4 mutations. EBV-positive tumors showed enrichment in chemokine/cytokine signaling pathways and altered immune cell composition, leading to poor prognosis.
Review
Pathology
Dauod Arif, Tetyana Mettler, Oyedele A. Adeyi
Summary: The article discusses the diagnostic challenges of hepatocellular carcinoma and emphasizes the importance of histopathological diagnosis in certain patient populations. It also highlights the limitations of high serum AFP levels in HCC diagnosis and calls for physicians to be vigilant in avoiding misdiagnosis.
Article
Multidisciplinary Sciences
Sagi Abelson, Andy G. X. Zeng, Ido Nofech-Mozes, Ting Ting Wang, Stanley W. K. Ng, Mark D. Minden, Trevor J. Pugh, Philip Awadalla, Liran Shlush, Tracy Murphy, Steven M. Chan, John E. Dick, Scott Bratman
Article
Multidisciplinary Sciences
Arvind Singh Mer, Emily M. Heath, Seyed Ali Madani Tonekaboni, Nergiz Dogan-Artun, Sisira Kadambat Nair, Alex Murison, Laura Garcia-Prat, Liran Shlush, Rose Hurren, Veronique Voisin, Gary D. Bader, Corey Nislow, Mattias Rantalainen, Soren Lehmann, Mark Gower, Cynthia J. Guidos, Mathieu Lupien, John E. Dick, Mark D. Minden, Aaron D. Schimmer, Benjamin Haibe-Kains
Summary: The molecular heterogeneity of AML poses challenges for prognosis and therapy. NPM1 mutated AML is heterogeneous, with two subtypes exhibiting distinct molecular characteristics, differentiation state, patient survival, and drug response.
NATURE COMMUNICATIONS
(2021)
Article
Cell & Tissue Engineering
Amit Subedi, Qiang Liu, Dhanoop M. Ayyathan, David Sharon, Severine Cathelin, Mohsen Hosseini, Changjiang Xu, Veronique Voisin, Gary D. Bader, Angelo D'Alessandro, Eric R. Lechman, John E. Dick, Mark D. Minden, Jean C. Y. Wang, Steven M. Chan
Summary: The study identified that NAMPT inhibitors selectively kill LSCs in AML while sparing normal hematopoietic stem cells and found that SREBP signaling plays a key role in NAMPT inhibitor-induced apoptosis. The work demonstrates altered lipid homeostasis as a crucial factor in inducing apoptosis by NAMPT inhibition and highlights NAMPT inhibition as a promising therapeutic strategy for targeting LSCs in AML.
Article
Hematology
Berenice Meza-Leon, Dita Gratzinger, Alicia G. Aguilar-Navarro, Fany G. Juarez-Aguilar, Vivienne I. Rebel, Emina Torlakovic, Louise E. Purton, Elisa M. Dorantes-Acosta, Argelia Escobar-Sanchez, John E. Dick, Eugenia Flores-Figueroa
Summary: Highly comparable distribution of bone marrow mesenchymal stromal cells among mouse, human, and dog suggests the validity of using mouse and dog as a surrogate experimental model for hematopoietic stem cell-BMSC interactions. However, further study is needed to explore the distinct differences in adipocyte and megakaryocyte microenvironment content of mouse bone marrow and how they might influence hematopoietic stem cell interactions compared to humans.
EXPERIMENTAL HEMATOLOGY
(2021)
Article
Multidisciplinary Sciences
Kimberly Skead, Armande Ang Houle, Sagi Abelson, Mawusse Agbessi, Vanessa Bruat, Boxi Lin, David Soave, Liran Shlush, Stephen Wright, John Dick, Quaid Morris, Philip Awadalla
Summary: The authors investigate how the interplay of positive and negative selection influences AML progression using deep learning and population genetics models. They find that purifying selection plays a critical role in preventing disease-predisposing clones from rising to dominance.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Erwin M. Schoof, Benjamin Furtwangler, Nil Uresin, Nicolas Rapin, Simonas Savickas, Coline Gentil, Eric Lechman, Ulrich auf Dem Keller, John E. Dick, Bo T. Porse
Summary: The study demonstrates a comprehensive experimental and computational workflow that establishes global single-cell mass spectrometry-based proteomics as a tool for large-scale single-cell analyses, enabling the exploration of cellular heterogeneity. The approach presented is capable of consistently quantifying around 1000 proteins per cell within limited instrument time.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Lindsey E. Montefiori, Sonja Bendig, Zhaohui Gu, Xiaolong Chen, Petri Polonen, Xiaotu Ma, Alex Murison, Andy Zeng, Laura Garcia-Prat, Kirsten Dickerson, Ilaria Iacobucci, Sherif Abdelhamed, Ryan Hiltenbrand, Paul E. Mead, Cyrus M. Mehr, Beisi Xu, Zhongshan Cheng, Ti-Cheng Chang, Tamara Westover, Jing Ma, Anna Stengel, Shunsuke Kimura, Chunxu Qu, Marcus B. Valentine, Marissa Rashkovan, Selina Luger, Mark R. Litzow, Jacob M. Rowe, Monique L. den Boer, Victoria Wang, Jun Yin, Steven M. Kornblau, Stephen P. Hunger, Mignon L. Loh, Ching-Hon Pui, Wenjian Yang, Kristine R. Crews, Kathryn G. Roberts, Jun J. Yang, Mary Relling, William E. Evans, Wendy Stock, Elisabeth M. Paietta, Adolfo A. Ferrando, Jinghui Zhang, Wolfgang Kern, Torsten Haferlach, Gang Wu, John E. Dick, Jeffery M. Klco, Claudia Haferlach, Charles G. Mullighan
Summary: This study identified a subgroup of acute leukemias with ambiguous lineage expressing myeloid, T lymphoid, and stem cell markers, driven by aberrant allele-specific deregulation of the master transcription factor BCL11B. Chromosomal rearrangements and focal amplifications leading to superenhancer generation were identified as mechanisms behind the oncogenic deregulation of BCL11B. This ectopic expression of BCL11B in hematopoietic cells mediated by enhancer hijacking was suggested as an oncogenic driver of human lineage-ambiguous leukemia.
Article
Biochemical Research Methods
Jessie J. F. Medeiros, Jose-Mario Capo-Chichi, Liran Shlush, John E. Dick, Andrea Arruda, Mark D. Minden, Sagi Abelson
Summary: The study introduced a computational method called SmMIP-tools, which can detect single nucleotide variants and short insertions and deletions from smMIP-based sequencing. Experimental results showed near-perfect performance in controlled experiments and outperformed other commonly used computational methods.
Article
Hematology
Lucas C. M. Arruda, Arwen Stikvoort, Melanie Lambert, Liqing Jin, Laura Sanchez Rivera, Renato M. P. Alves, Tales Rocha de Moura, Carsten Mim, Soren Lehmann, Rebecca Axelsson-Robertson, John E. Dick, Jonas Mattsson, Bjorn Onfelt, Mattias Carlsten, Michael Uhlin
Summary: A CD34-specific bi-specific T-cell engager (BTE) has been designed and tested in this study, demonstrating its ability to activate T cells and kill leukemia cells with the CD34(+) phenotype. In vivo experiments showed that the CD34-specific BTE had robust anti-tumor effects.
Article
Endocrinology & Metabolism
Gavin Fredrickson, Fanta Barrow, Katrina Dietsche, Preethy Parthiban, Saad Khan, Sacha Robert, Maya Demirchian, Hailey Rhoades, Haiguang Wang, Oyedele Adeyi, Xavier S. Revelo
Summary: This study found that HIIT was more effective than MIT in improving the progression of NASH, with HIIT outperforming MIT in reducing hepatic steatosis, improving whole-body glucose tolerance, and ameliorating liver inflammation and fibrosis.
MOLECULAR METABOLISM
(2021)
Article
Hematology
Stanley W. K. Ng, Tracy Murphy, Ian King, Tong Zhang, Michelle Mah, Zhibin Lu, Natalie Stickle, Narmin Ibrahimova, Andrea Arruda, Amanda Mitchell, Ming Mai, Rong He, Bindu Swapna Madala, David S. Viswanatha, John E. Dick, Steven Chan, Carl Virtanen, Mark D. Minden, Timothy Mercer, Tracy Stockley, Jean C. Y. Wang
Summary: The LSC17 gene expression score can predict survival outcomes and treatment response in AML patients, enabling personalized risk-adapted management.
Article
Pathology
Justin Bateman, Chimaobi Anugwom, Yan Zhou, Nicholas Lim, Oyedele Adeyi
Summary: This study compares the clinicopathologic features of late-onset rejection (LOR) and finds that different types of LOR have overlapping patterns, but they respond well to antirejection treatments.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2023)
Article
Oncology
Jeff A. Wintersinger, Stephanie M. Dobson, Ethan Kulman, Lincoln D. Stein, John E. Dick, Quaid Morris
Summary: Pairtree is a new method that uses DNA-sequencing data to construct more accurate and detailed clone trees, revealing the evolutionary history of cancer and providing insights for treatment.
BLOOD CANCER DISCOVERY
(2022)
Article
Oncology
Robert J. Vanner, Stephanie M. Dobson, Olga Gan, Jessica McLeod, Erwin M. Schoof, Ildiko Grandal, Jeff A. Wintersinger, Laura Garcia-Prat, Mohsen Hosseini, Stephanie Z. Xie, Liqing Jin, Nathan Mbong, Veronique Voisin, Michelle Chan-Seng-Yue, James A. Kennedy, Esme Waanders, Quaid Morris, Bo Porse, Steven M. Chan, Cynthia J. Guidos, Jayne S. Danska, Mark D. Minden, Charles G. Mullighan, John E. Dick
Summary: In B-ALL CNS disease, the leptomeningeal environment selects cells with unique functional dependencies. Pharmacologic inhibition of mRNA translation signaling treats CNS disease and offers a new therapeutic approach for this condition.
BLOOD CANCER DISCOVERY
(2022)