Article
Oncology
Sara da Mata, Teresa Franchi-Mendes, Sofia Abreu, Bruno Filipe, Sonia Morgado, Marta Mesquita, Cristina Albuquerque, Ricardo Fonseca, Vitor E. Santo, Erwin R. Boghaert, Isadora Rosa, Catarina Brito
Summary: The study outlines the development of a colorectal cancer ex vivo model using fresh tumor samples, highlighting the preservation of original tumor genetic features and partial retention of architecture and non-malignant cells in culture. The model shows promise for use in pre- and co-clinical settings.
Editorial Material
Oncology
Christopher G. Hubert, Jeremy N. Rich
Summary: Tumors are dynamic ecosystems with heterogeneous neoplastic cells and diverse stromal elements, posing challenges for ex vivo predictive modeling. Single-cell analysis reveals that patient-derived explants can replicate the diversity of tumor cells and transient stromal cell types found in patient surgical specimens, suggesting their value as tumor models.
Review
Oncology
Abby R. Templeton, Penny L. Jeffery, Patrick B. Thomas, Mahasha P. J. Perera, Gary Ng, Alivia R. Calabrese, Clarissa Nicholls, Nathan J. Mackenzie, Jack Wood, Laura J. Bray, Ian Vela, Erik W. Thompson, Elizabeth D. Williams
Summary: This article investigates the use of patient-derived explants (PDEs) in cancer treatment, revealing their limited longevity which may not be suitable for integrating personalized treatment options. Therefore, improving the longevity of PDEs is crucial to enhance the feasibility and effectiveness of personalized therapies.
FRONTIERS IN ONCOLOGY
(2021)
Article
Engineering, Biomedical
Terence Tieu, Swati Irani, Kayla L. Bremert, Natalie K. Ryan, Marcin Wojnilowicz, Madison Helm, Helmut Thissen, Nicolas H. Voelcker, Lisa M. Butler, Anna Cifuentes-Rius
Summary: Research in nanomedicine has predominantly focused on cancer therapeutics, but many nanoscale drug delivery systems have not yet translated to clinical settings. Lack of key clinical tumor elements in current in vivo models hinders assessment of drug response. This study uses patient-derived explants (PDE) for evaluating the efficacy of siRNA delivery systems.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Oncology
Margaret M. Centenera, Andrew D. Vincent, Max Moldovan, Hui-Ming Lin, David J. Lynn, Lisa G. Horvath, Lisa M. Butler
Summary: There is a growing trend towards utilizing more biologically relevant pre-clinical models of prostate cancer to improve drug and biomarker discovery, with patient-derived explant culture being a promising innovative model. These models retain critical elements of the original tumor, reflecting the complexity and heterogeneity of prostate cancer, and offer potential for novel drug target and biomarker discovery through multi-omics analysis.
Article
Biology
Shiki Fujino, Norikatsu Miyoshi, Aya Ito, Rie Hayashi, Masayoshi Yasui, Chu Matsuda, Masayuki Ohue, Masafumi Horie, Shinichi Yachida, Jun Koseki, Teppei Shimamura, Tsuyoshi Hata, Takayuki Ogino, Hidekazu Takahashi, Mamoru Uemura, Tsunekazu Mizushima, Yuichiro Doki, Hidetoshi Eguchi
Summary: High expression of POU5F1 is associated with colorectal cancer recurrence and liver metastasis. POU5F1-expressing cells in two-dimensional culture have cancer stem cell abilities and can form liver metastases in vivo. Single-cell RNA sequencing identifies heterogeneous populations derived from POU5F1-expressing cells and the Wnt signaling pathway is enriched in these cells.
COMMUNICATIONS BIOLOGY
(2023)
Article
Engineering, Biomedical
Yong Hun Jung, Dong-Hee Choi, Kyungwon Park, Sat-Byol Lee, Jonghun Kim, Hyunho Kim, Hyun-Woo Jeong, Ji Hun Yang, Jin-A Kim, Seok Chung, Byung Soh Min
Summary: This study demonstrates a scalable organoid production platform that can produce functionally complex and uniform organoids. Hydro-organoids produced on the new platform show better consistency in drug screening and have the potential to be used in high-throughput assays and predicting clinical outcomes.
Article
Oncology
Lifeng Chen, Bo Tian, Wen Liu, Haitao Liang, Yong You, Weizhen Liu
Summary: The study established a predictive 5-Fu resistance molecular biomarker derived from colorectal cancer organoids, showing significant diversity in drug sensitivity and identified differentially expressed genes associated with 5-Fu resistance. The drug-resistant score model based on five genes developed from patient-derived organoids was found to be an independent prognostic factor for overall survival in CRC patients and could predict survival in different subgroups.GetEnumeratorFurther analysis also revealed differences in molecular pathways, tumor mutational burden, immune response-related pathways, immune score, stromal score, and immune cell proportion between patients with high and low drug-resistant scores in the TCGA-CRC cohort.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Gabrielle T. Parkinson, Simona Salerno, Parmida Ranji, Joakim Hakansson, Yalda Bogestal, Yvonne Wettergren, Anders Stahlberg, Elinor Bexe Lindskog, Goran Landberg
Summary: The study utilized patient-derived scaffolds to model the heterogeneous nature of colorectal cancer and found significant differences in gene and protein expression between cells grown in scaffolds compared to those in traditional 2D culture. These findings suggest a potential personalized diagnostic tool with implications for individual patient clinical outcomes.
Review
Surgery
Milton Mui, Molly Clark, Tamara M. S. H. Vu, Nicholas Clemons, Frederic Hollande, Sara Roth, Robert Ramsay, Michael Michael, Alexander G. Heriot, Joseph C. H. Kong
Summary: This scoping review evaluates the utility of patient-derived explant cultures as a preclinical model in colorectal cancer. The results show that patient-derived explants, with the preservation of the tumor microenvironment and tumor heterogeneity, have the potential to identify reliable biomarkers, treatment resistance mechanisms, and novel therapeutic agents.
LANGENBECKS ARCHIVES OF SURGERY
(2023)
Article
Medicine, Research & Experimental
Mohamed Elbadawy, Kimika Hayashi, Hiromi Ayame, Yusuke Ishihara, Amira Abugomaa, Makoto Shibutani, Shim-Mo Hayashi, Shoichi Hazama, Hiroko Takenouchi, Masao Nakajima, Ryouichi Tsunedomi, Nobuaki Suzuki, Hiroaki Nagano, Yuta Shinohara, Masahiro Kaneda, Hideyuki Yamawaki, Tatsuya Usui, Kazuaki Sasaki
Summary: Despite the adverse effects, chemotherapy is commonly used for colorectal cancer treatment. Developing supplement preparations targeting cancer stem cells is necessary. In this study, amorphous curcumin (AC) showed anti-carcinogenic properties against human CRC organoids by inhibiting cell viability, cell cycle, and CSC marker expressions while synergizing with anti-cancer drugs.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Rebeca Kelly, Diego Aviles, Catriona Krisulevicz, Krystal Hunter, Lauren Krill, David Warshal, Olga Ostrovsky
Summary: High mortality rates in ovarian cancer have been associated with recurrence, metastasis, and chemoresistance, which involve genetic changes and epigenetic aberrations. Adipose-derived stem cells from the omentum (O-ASCs) play a crucial role in supporting tumor growth and dissemination through epigenetic abnormalities. Natural epigenetic compounds, such as EGCG and I3C, show promise in reprogramming aberrant epigenetic modifications and suppressing ovarian cancer growth.
Article
Oncology
Colin S. Wood, Kathryn A. F. Pennel, Holly Leslie, Assya Legrini, Andrew J. Cameron, Lydia Melissourgou-Syka, Jean A. Quinn, Hester C. van Wyk, Jennifer Hay, Antonia K. Roseweir, Colin Nixon, Campbell S. D. Roxburgh, Donald C. McMillan, Andrew V. Biankin, Owen J. Sansom, Paul G. Horgan, Joanne Edwards, Colin W. Steele, Nigel B. Jamieson
Summary: Strong immune responses in primary colorectal cancer are associated with better patient survival. However, identifying patients with good prognosis after surgery for colorectal cancer liver metastases remains challenging. This study aims to determine the practical application of immune cell infiltration and stromal content in predicting outcome and investigate the underlying functional biology.
Article
Chemistry, Multidisciplinary
Yuanyuan Zhao, Bing Zhang, Yiming Ma, Fuqiang Zhao, Jianan Chen, Bingzhi Wang, Hua Jin, Fulai Zhou, Jiawei Guan, Qian Zhao, Hongying Wang, Qian Liu, Fangqing Zhao, Xia Wang
Summary: This study establishes an innovative model for studying intratumor functional heterogeneity in personalized oncology. The model efficiently replicates the genomic landscape of patient-derived cancer cells and tumors, while retaining the histological architecture. Moreover, it is found that tumorigenic cancer cells do not necessarily possess stem cell characteristics.
Article
Biochemistry & Molecular Biology
Gregorio Di Franco, Alice Usai, Margherita Piccardi, Perla Cateni, Matteo Palmeri, Luca Emanuele Pollina, Raffaele Gaeta, Federica Marmorino, Chiara Cremolini, Luciana Dente, Alessandro Massolo, Vittoria Raffa, Luca Morelli
Summary: The use of zebrafish embryos (zPDX) has gained popularity in personalized medicine for predicting chemotherapy response in colorectal cancer patients. In this co-clinical trial, zPDXs exposed to different chemotherapy regimens exhibited significant reductions in tumor size compared to controls. The response of zPDXs correlated with the outcomes in a subset of metastatic colorectal cancer patients, indicating the potential for zPDX models in personalized medicine.
Review
Biotechnology & Applied Microbiology
Michael A. Morse, Erika J. Crosby, Jeremy Force, Takuya Osada, Amy C. Hobeika, Zachary C. Hartman, Peter Berglund, Jonathan Smith, H. Kim Lyerly
Summary: Therapeutic cancer vaccines use different platforms, such as messenger RNAs (mRNA), for antigen delivery. Self-replicating RNAs (srRNA) increase epitope expression and have been derived from positive-strand RNA viruses. Clinical stage srRNA vectors have shown positive results in encoding tumor antigens and adverse events have been minimal. Recent development of fully synthetic srRNA technologies shows promise for future vaccines.
CANCER GENE THERAPY
(2023)
Editorial Material
Biotechnology & Applied Microbiology
Herbert Kim Lyerly
CANCER GENE THERAPY
(2023)
Correction
Medicine, General & Internal
Divya L. Dayanidhi, Jason A. Somarelli, John B. Mantyh, Gabrielle Rupprecht, Roham Salman Roghani, Sophia Vincoff, Iljin Shin, Yiquan Zhao, So Young Kim, Shannon McCall, Jiyong Hong, David S. Hsu
FRONTIERS IN MEDICINE
(2023)
Editorial Material
Oncology
Erika J. Crosby, Zachary C. Hartman, H. Kim Lyerly
Summary: A vaccine targeted at HER2 can prime T cells for ex vivo expansion and adoptive transfer with minimal toxicity. This treatment modality can lead to intramolecular epitope spreading in most patients and potentially improve outcomes for patients with metastatic HER2-positive breast cancer.
CLINICAL CANCER RESEARCH
(2023)
Correction
Urology & Nephrology
Qing Cheng, William Butler, Yinglu Zhou, Hong Zhang, Lu Tang, Kathryn Perkinson, Xufeng Chen, Xiaoyin Sara Jiang, Shannon J. McCall, Brant A. Inman, Jiaoti Huang
Editorial Material
Gastroenterology & Hepatology
Katherine S. Garman, Biswa P. D. Purkayastha, Joyce A. Hogue, Ryan Fecteau, Kishore Guda, Amitabh Chak
Article
Oncology
Takuya Osada, Xiaoning Jiang, Yuhang Zhao, Mengyue Chen, Benjamin C. Kreager, Huaiyu Wu, Howuk Kim, Jun Ren, Joshua Snyder, Pei Zhong, Michael A. Morse, H. Kim Lyerly
Summary: Mechanical high-intensity focused ultrasound (M-HIFU), including histotripsy, is a non-invasive and non-thermal ablation technology that has shown initial safety and efficacy in treating malignant liver tumors. In addition to tissue ablation, M-HIFU has the potential to stimulate local and systemic antitumor immune response in preclinical models and clinical trials.
INTERNATIONAL JOURNAL OF HYPERTHERMIA
(2023)
Review
Oncology
Shannon J. Mccall, Irina A. Lubensky, Christopher A. Moskaluk, Anil Parwani, Kiley Radin, Nilsa C. Ramirez, Zachery Von Menchhofen, Mary K. Washington, Virginia A. Livolsi
Summary: The Cooperative Human Tissue Network, established in 1987, supports a coordinated national effort in cancer research by collecting and distributing high quality human tissues. The network has expanded its offerings to include various types of tissue samples, blood and body fluid samples, as well as clinical datasets. Over 1,375,041 biospecimens have been distributed through the network, serving 889 active investigators in 2021. This article reviews the network's 35-year history, highlights changes in the program over the past 15 years, and provides insights into its operations and scientific achievements.
MOLECULAR CANCER THERAPEUTICS
(2023)
Article
Medicine, Research & Experimental
Timothy N. Trotter, Carina E. Dagotto, Delila Serra, Tao Wang, Xiao Yang, Chaitanya R. Acharya, Junping Wei, Gangjun Lei, H. Kim Lyerly, Zachary C. Hartman
Summary: This study reveals that dormant tumor cells can evade immune detection by utilizing tolerance mechanisms to alter the T cell microenvironment. The researchers found that dormant tumor cells can initiate immune responses but also promote the accumulation of regulatory T cells, inhibiting the activity of CD8+ killer T cells. Additionally, a protein called DKK3 derived from the tumor cells is critical for this suppression of the immune system and is associated with poor survival and immunosuppression in human breast cancers. Targeting these mechanisms, such as DKK3, may render dormant tumors susceptible to immunotherapies.
Meeting Abstract
Oncology
Zelanna Goldberg, Christian Maine, Gabrielle P. Dailey, Christine Domingo, Gaelle Picarda, Hunter Little, Annie Chou, Jessica Sparks, Darina Spasova, Shigeki Miyake-Stoner, Christopher A. Rabiola, Erika J. Crosby, Zachary C. Hartman, Herbert K. Lyerly, Nathaniel Wang, Parinaz Aliahmad
Meeting Abstract
Oncology
Zelanna Goldberg, Christian Maine, Gabrielle P. Dailey, Christine Domingo, Gaelle Picarda, Hunter Little, Annie Chou, Jessica Sparks, Darina Spasova, Shigeki Miyake-Stoner, Zachary C. Hartman, Christopher A. Rabiola, Erika J. Crosby, Herbert K. Lyerly, Nathaniel Wang, Parinaz Aliahmad
Meeting Abstract
Oncology
Bin-Jin Hwang, Erika Crosby, Timothy Trotter, Li-Chung Tsao, Tao Wang, Xiao Yang, Herbert Kim Lyerly, Zachary Hartman
Meeting Abstract
Medicine, Research & Experimental
Shannon McCall, Rodrigo Chuaqui, Irina Lubensky, Kiley Radin, Christopher Moskaluk, Nilsa Ramirez, Anil Parwani, Mary Washington, Virginia LiVolsi
LABORATORY INVESTIGATION
(2023)
Meeting Abstract
Oncology
Timothy N. Trotter, Delila Serra, Carina Dagotto, Tao Wang, Xiao Yang, Junping Wei, Gangjun Lei, H. Kim Lyerly, Zachary C. Hartman
Review
Biotechnology & Applied Microbiology
Anchit Bhagat, Herbert K. Lyerly, Michael A. Morse, Zachary C. Hartman
Summary: This review summarizes the progress in the development of CEA vaccines, demonstrating their ability to elicit immune response and improve clinical outcomes, but further confirmation is needed.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2023)