Article
Multidisciplinary Sciences
Guo-Hua Wang, Cheng-Cheng Liang, Bing-Zhi Li, Xin-Ze Du, Wen-Zhen Zhang, Gong Cheng, Lin-Sen Zan
Summary: This study assessed the accuracy of sixteen commonly used internal reference genes in skeletal muscle-derived satellite cells of Qinchuan cattle at different stages of proliferation and induction of differentiation, and determined the most suitable gene combinations. The results provide a foundation for selecting reference genes for qRT-PCR during the proliferation and induction of differentiation of bovine skeletal muscle-derived satellite cells.
SCIENTIFIC REPORTS
(2022)
Article
Agriculture, Dairy & Animal Science
Guangjie Xie, Lu Tang, Yaqiu Lin, Mingfeng Jiang, Qing Xu, Jiangjiang Zhu, Qingyong Meng, Yong Wang
Summary: In this study, suitable reference genes for normalizing gene expression data in the adipose tissue of Jianzhou da'er goat were identified, with HSP90 as the most stable reference gene. A combination of HSP90, ALAS1, and RPLP0 was also found to be effective for normalization of target genes.
JOURNAL OF APPLIED ANIMAL RESEARCH
(2021)
Article
Veterinary Sciences
Le Zhao, Haili Yang, Xingchun Li, Yumei Zhou, Taolu Liu, Yongju Zhao
Summary: This study evaluated candidate reference genes for the early whiting mechanism of goat brown adipose tissue (BAT) and identified the most stable reference genes. These findings contribute to further understanding the molecular mechanisms underlying BAT whiting in goats and provide important reference genes for research in this field.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Physiology
Wei Na, Yuxiang Wang, Pengfei Gong, Xinyang Zhang, Ke Zhang, Hui Zhang, Ning Wang, Hui Li
Summary: The study utilized the GeNorm algorithm to analyze the expression stability of 14 candidate reference genes in the abdominal adipose tissue of broilers, finding that TBP and HMBS genes exhibited the highest stability at each stage.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Marin Bencevic, Irena Galic, Marija Habijan, Aleksandra Pizurica
Summary: This paper provides a comprehensive and critical overview of research on the segmentation and quantification methods of epicardial and pericardial adipose tissues. It evaluates the effectiveness of these methods, compares them, and discusses the future challenges in this field. Additionally, it gives an overview of the clinical significance and terminology of these adipose tissues.
APPLIED SCIENCES-BASEL
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Xiaogang Li, Yu Sun, Lisheng Xu, Stephen E. Greenwald, Libo Zhang, Rongrong Zhang, Hongrui You, Benqiang Yang
Summary: The study aimed to establish a fully automatic framework for segmented and quantifying epicardial fat in CCTA scans. The developed method compared favorably with assessments from two independent experts, showing high accuracy and reliability in EAT volume computation.
Article
Multidisciplinary Sciences
Iokfai Cheang, Xu Zhu, Xin Yue, Yuan Tang, Yujie Gao, Xinyi Lu, Shi Shi, Shengen Liao, Wenming Yao, Yanli Zhou, Haifeng Zhang, Yinsu Zhu, Yi Xu, Xinli Li
Summary: The purpose of this study was to investigate the prognostic values of ventricle epicardial fat volume (EFV) in patients with chronic heart failure (CHF) using cardiac magnetic resonance. A total of 516 CHF patients were recruited, and 136 (26.4%) of them experienced major adverse cardiovascular events (MACE) within a median follow-up of 24 months. The study found that EFV was significantly associated with MACE and showed promising predictive ability for MACE at different time points.
Review
Biochemistry & Molecular Biology
Sara Leo, Elena Tremoli, Letizia Ferroni, Barbara Zavan
Summary: Obesity and insulin resistance are risk factors for cardiovascular diseases, associated with inflamed and defective adipose tissue. Extracellular vesicles, involved in insulin resistance, diabetes, and CVDs, play a role in the onset and development of these pathologies. Inflammation triggers the transition of adipose tissue secretome, leading to an increase in pro-inflammatory mediators.
Article
Biochemistry & Molecular Biology
Yaqiong Wu, Chunhong Zhang, Haiyan Yang, Lianfei Lyu, Weilin Li, Wenlong Wu
Summary: Through studying blackberry and raspberry cultivars, RuEEF1A and Ru18S were selected as stable internal reference genes, providing a reference for further research on key functional genes in these two plants and an effective tool for differential gene expression analysis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Maria Noelia Ulrich, Esteban Muniz-Padilla, Alejandra Corach, Esteban Hopp, Daniela Tosto
Summary: This study evaluated the stability of different reference genes of glyphosate-resistant S. halepense, finding that PP2A and ARI8 were the most stable genes. The results provide valuable information for gene expression analysis under glyphosate stress and will facilitate resistance mechanism studies in this weed species.
Article
Biochemistry & Molecular Biology
Jesus M. Perez-Gomez, Francisco Porcel-Pastrana, Marina De La Luz-borrero, Antonio J. Montero-Hidalgo, Enrique Gomez-Gomez, Aura D. Herrera-Martinez, Rocio Guzman-Ruiz, Maria M. Malagon, Manuel D. Gahete, Raul M. Luque
Summary: Obesity is a metabolic disorder associated with various comorbidities, including certain types of cancers. Prostate cancer has been suggested to have a causal relationship with obesity. Periprostatic adipose tissue has gained attention as a key player in the pathophysiological interaction between prostate cancer and obesity. This study identified the best internal reference genes to be used in gene expression studies in human periprostatic adipose tissue under prostate cancer and obesity conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chaofan Jiang, Lin Zhou, Qingchen Zhao, Mengke Wang, Sirui Shen, Te Zhao, Kaidi Cui, Leiming He
Summary: This study validated the expression stability of seven candidate reference genes in Sclerotium rolfsii under different conditions and recommended suitable reference gene combinations for RT-qPCR normalization. The results showed that using one or two reference genes to normalize the expression of target genes in S. rolfsii did not significantly differ.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Horticulture
Bing He, Hui Chen, Pibiao Shi, Fengqin Hu, Wenjing Song, Lin Meng, Yuanda Lv
Summary: In this study, novel housekeeping and tissue-specific genes were identified in quinoa using different methods, and their expression stability was further evaluated. The results showed that MIF, RGGA, VATE, and UBA2B were the most stable candidate housekeeping genes, while leaf and root-specific genes were also identified. Gene Ontology analysis revealed the functions and enrichment of these genes in quinoa.
Article
Cardiac & Cardiovascular Systems
Belinda Spoto, Ernesto Di Betta, Patrizia Pizzini, S. Lonardi, F. Mallamaci, G. Tripepi, Mehmet Kanbay, Giovanni Cancarini, Carmine Zoccali
Summary: This study compared individuals with metabolically healthy obesity (MHO) and metabolically unhealthy obesity (MUO) and found no significant differences in circulating levels of cytokines and adipokines, as well as the expression of inflammatory genes in adipose tissue. The results suggest that metabolically healthy obese individuals have similar inflammation profiles to metabolically unhealthy obese individuals.
NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yifan Li, Shuni Song, Yu Sun, Nan Bao, Benqiang Yang, Lisheng Xu
Summary: This paper presents a new lightweight method for epicardial adipose tissue (EAT) segmentation network, which achieves higher segmentation accuracy with less computation and mitigates the problem of false-positive segmentation. Experimental results on real dataset and cardiac fat database demonstrate the effectiveness of the proposed method.
Article
Endocrinology & Metabolism
Sebastien M. Labbe, Alexandre Caron, Kanta Chechi, Mathieu Laplante, Roger Lecomte, Denis Richard
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2016)
Review
Physiology
Kanta Chechi, Wouter van Marken Lichtenbelt, Denis Richard
JOURNAL OF APPLIED PHYSIOLOGY
(2018)
Article
Endocrinology & Metabolism
Ying Liu, Subat Turdi, Taesik Park, Nicholas J. Morris, Yves Deshaies, Aimin Xu, Gary Sweeney
Article
Cardiac & Cardiovascular Systems
Kanta Chechi, Pierre-Gilles Blanchard, Patrick Mathieu, Yves Deshaies, Denis Richard
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2013)
Article
Biochemistry & Molecular Biology
Pierre-Gilles Blanchard, William T. Festuccia, Vanessa P. Houde, Philippe St-Pierre, Sophie Brule, Veronique Turcotte, Marie Cote, Kerstin Bellmann, Andre Marette, Yves Deshaies
JOURNAL OF LIPID RESEARCH
(2012)
Article
Endocrinology & Metabolism
K. Chechi, J. Nedergaard, D. Richard
Review
Endocrinology & Metabolism
Kanta Chechi, Andre C. Carpentier, Denis Richard
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2013)
Article
Endocrinology & Metabolism
Valerie Turcot, Andre Tchernof, Yves Deshaies, Louis Perusse, Alexandre Belisle, Picard Marceau, Frederic-Simon Hould, Stefane Lebel, Marie-Claude Vohl
DIABETOLOGY & METABOLIC SYNDROME
(2013)
Article
Multidisciplinary Sciences
Kanta Chechi, Pierre Voisine, Patrick Mathieu, Mathieu Laplante, Sebastian Bonnet, Frederic Picard, Philippe Joubert, Denis Richard
SCIENTIFIC REPORTS
(2017)
Article
Cardiac & Cardiovascular Systems
Jolyane Meloche, Antony Courchesne, Marjorie Barrier, Sophie Carter, Malik Bisserier, Roxane Paulin, Jean-Francois Lauzon-Joset, Sandra Breuils-Bonnet, Eve Tremblay, Sabrina Biardel, Christine Racine, Christian Courture, Pierre Bonnet, Susan M. Majka, Yves Deshaies, Frederic Picard, Steeve Provencher, Sebastien Bonnet
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2013)
Article
Biochemistry & Molecular Biology
Yeela Talmor-Barkan, Noam Bar, Aviv A. Shaul, Nir Shahaf, Anastasia Godneva, Yuval Bussi, Maya Lotan-Pompan, Adina Weinberger, Alon Shechter, Chava Chezar-Azerrad, Ziad Arow, Yoav Hammer, Kanta Chechi, Sofia K. Forslund, Sebastien Fromentin, Marc-Emmanuel Dumas, S. Dusko Ehrlich, Oluf Pedersen, Ran Kornowski, Eran Segal
Summary: Multi-omic profiling of patients with coronary artery disease reveals links between alterations in the serum metabolome with genetics, diet, and the microbiome. The study found that these metabolomic alterations are associated with metabolic impairment preceding clinically overt coronary artery disease. The results highlight the importance of understanding risk-factor heterogeneity in coronary artery disease through the serum metabolome.
Article
Biochemistry & Molecular Biology
Sebastien Fromentin, Sofia K. Forslund, Kanta Chechi, Judith Aron-Wisnewsky, Rima Chakaroun, Trine Nielsen, Valentina Tremaroli, Boyang Ji, Edi Prifti, Antonis Myridakis, Julien Chilloux, Petros Andrikopoulos, Yong Fan, Michael T. Olanipekun, Renato Alves, Solia Adiouch, Noam Bar, Yeela Talmor-Barkan, Eugeni Belda, Robert Caesar, Luis Pedro Coelho, Gwen Falony, Soraya Fellahi, Pilar Galan, Nathalie Galleron, Gerard Helft, Lesley Hoyles, Richard Isnard, Emmanuelle Le Chatelier, Hanna Julienne, Lisa Olsson, Helle Krogh Pedersen, Nicolas Pons, Benoit Quinquis, Christine Rouault, Hugo Roume, Joe-Elie Salem, Thomas S. B. Schmidt, Sara Vieira-Silva, Peishun Li, Maria Zimmermann-Kogadeeva, Christian Lewinter, Nadja B. Sondertoft, Tue H. Hansen, Dominique Gauguier, Jens Peter Gotze, Lars Kober, Ran Kornowski, Henrik Vestergaard, Torben Hansen, Jean-Daniel Zucker, Serge Hercberg, Ivica Letunic, Fredrik Backhed, Jean-Michel Oppert, Jens Nielsen, Jeroen Raes, Peer Bork, Michael Stumvoll, Eran Segal, Karine Clement, Marc-Emmanuel Dumas, S. Dusko Ehrlich, Oluf Pedersen
Summary: This study investigates the alterations of the metabolome and microbiome from dysmetabolic conditions to ischemic heart disease (IHD). The findings suggest that major changes in the gut microbiome and metabolome may occur long before the clinical onset of IHD. Specific microbiome and metabolome features associated with IHD could better differentiate individuals with IHD from healthy individuals, indicating their pathophysiological relevance.
Article
Multidisciplinary Sciences
Thushan de Silva, Guihai Liu, Benjamin B. Lindsey, Danning Dong, Shona C. Moore, Nienyun Sharon Hsu, Dhruv Shah, Dannielle Wellington, Alexander J. Mentzer, Adrienn Angyal, Rebecca Brown, Matthew D. Parker, Zixi Ying, Xuan Yao, Lance Turtle, Susanna Dunachie, Mala K. Maini, Graham Ogg, Julian C. Knight, Yanchun Peng, Sarah L. Rowland-Jones, Tao Dong
Summary: Variants within dominant SARS-CoV-2 T cell epitopes have been identified through global sequence data analysis. These variants, independently arising in multiple lineages, result in loss of T cell recognition of specific epitopes. The findings demonstrate the potential for T cell evasion and emphasize the importance of ongoing surveillance for variants capable of escaping immune responses.
Article
Medicine, General & Internal
Thomas M. Drake, Aya M. Riad, Cameron J. Fairfield, Conor Egan, Stephen R. Knight, Riinu Pius, Hayley E. Hardwick, Lisa Norman, Catherine A. Shaw, Kenneth A. McLean, A. A. Roger Thompson, Antonia Ho, Olivia V. Swann, Michael Sullivan, Felipe Soares, Karl A. Holden, Laura Merson, Daniel Plotkin, Louise Sigfrid, Thushan I. de Silva, Michelle Girvan, Clare Jackson, Clark D. Russell, Jake Dunning, Tom Solomon, Gail Carson, Piero Olliaro, Jonathan S. Nguyen-Van-Tam, Lance Turtle, Annemarie B. Docherty, Peter J. M. Openshaw, J. Kenneth Baillie, Ewen M. Harrison, Malcolm G. Semple
Summary: This study found that complications and worse functional outcomes in patients admitted to hospital with COVID-19 are high, even in young, previously healthy individuals. Acute complications are associated with reduced ability to self-care at discharge, with neurological complications being associated with the worst functional outcomes. COVID-19 complications are likely to cause a substantial strain on health and social care in the coming years.
Article
Medicine, Research & Experimental
Kanta Chechi, Jinchu Vijay, Pierre Voisine, Patrick Mathieu, Yohan Bosse, Andre Tchernof, Elfin Grundberg, Denis Richard