Article
Microbiology
Xidian Tang, Yanfei Xie, Guanhua Li, Zhannur Niyazbekova, Shaofei Li, Jianjun Chang, Dekun Chen, Wentao Ma
Summary: The entry of ORFV into target cells is mediated by clathrin-mediated endocytosis and macropinocytosis, while caveolae-dependent endocytosis has minor effects. Additionally, ORFV entry into cells requires an acidic environment and the involvement of dynamin.
VETERINARY MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Hai-Vy Vo-Nguyen, Thanh-Tan Nguyen, Huyen-Trang Thi Vu, Thanh-Thao Thi Nguyen, Quoc-Cuong Hoang, Thuoc Linh Tran, Hieu Tran-Van
Summary: Hand, foot and mouth disease is a common viral infectious disease caused by enteroviruses, in this study, the potential of recombinant SCARB2 in preventing EV71 infection and transmission was evaluated; by cloning the scarb2 gene into pET22b vector and expressing in E. coli, a 100% recovery efficacy was achieved for refolded SCARB2 to potentially inhibit virus transmission.
IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A-SCIENCE
(2021)
Article
Microbiology
Wei Pan, Nie Hui, Hongmei Wang, Hongbin He
Summary: Bovine parainfluenza virus 3 enters MDBK cells via macropinocytosis and clathrin-mediated endocytosis, requiring acid-dependent entry and cathepsin L. Additionally, macropinocytosis is dependent on actin dynamics during BPIV3 infection.
VETERINARY MICROBIOLOGY
(2021)
Article
Virology
Wei Pan, Hui Nie, Hongmei Wang, Hongbin He
Summary: BPIV3 enters HeLa cells through the clathrin-mediated endocytosis pathway, requiring endosomal cathepsins but not a low pH. This provides novel insights into the invasion mechanism of Paramyxoviridae.
Article
Immunology
Ruihan Shi, Lei Hou, Li Wei, Jue Liu
Summary: Clathrin-mediated endocytosis serves as the initial step for effective viral infection by allowing viruses to enter cells. Adaptor proteins play a crucial role in this process, with their research shedding light on the viral life cycle and paving the way for novel therapeutics.
MICROBIAL PATHOGENESIS
(2021)
Article
Chemistry, Medicinal
Olaia Marti-Mari, Belen Martinez-Gualda, Irene Fernandez-Barahona, Alberto Mills, Rana Abdelnabi, Sam Noppen, Johan Neyts, Dominique Schols, Maria-Jose Camarasa, Fernando Herranz, Federico Gago, Ana San-Felix
Summary: Recently, we have discovered a novel family of compounds that are smaller in size and possess dual anti-HIV and anti-EV71 activities. Some of the derivatives showed greater potency and selectivity against HIV-1, HIV-2, and EV-A71 compared to the prototype compound AL-470. A fluorescent probe exhibited specific tropism for the intestines and lungs, which are important niches for the human microbiome in health and disease.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Virology
Yongzhe Zhu, Xiaohang Wang, Zhiwei He, Ping Zhao, Hao Ren, Zhongtian Qi
Summary: Infection of the central nervous system caused by enterovirus 71 (EV71) in hand-foot-and-mouth disease is the main cause of death. This study identified a new pathway by which EV71 enters human brain microvascular endothelial cells (HBMECs) and showed its dependence on ADP-ribosylation factor 6 (ARF6). Inhibition of ARF6 reduced EV71 infectivity in HBMECs and a specific inhibitor of ARF6 alleviated EV71 infection mortality in mice.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Veterinary Sciences
Ngo Thi Phuong Lan, Lizhen Wang, Wei Li, Xiaolan Hou, Wenchi Chang, Bo Wen, Shuizhong Han, Yan Chen, Xuefeng Qi, Jingyu Wang
Summary: The entry mechanism of fowl adenovirus serotype-4 (FAdV-4) into liver cells was investigated. The results showed that FAdV-4 entered cells through clathrin-mediated endocytosis dependent on cholesterol and macropinocytosis requiring PI3K. These findings provide important insights into the entry mechanisms of other avian adenoviruses.
VETERINARY RESEARCH
(2023)
Article
Virology
Shiqian Li, Dai Xiao, Yujia Zhao, Luwen Zhang, Rui Chen, Weizhe Liu, Yimin Wen, Yijie Liao, Yiping Wen, Rui Wu, Xinfeng Han, Qin Zhao, Senyan Du, Qigui Yan, Xintian Wen, Sanjie Cao, Xiaobo Huang
Summary: Using chemical inhibitors and protein knockdown methods, we identified that the entry of porcine deltacoronavirus (PDCoV) into PK-15 cells depends on caveolae-mediated endocytosis, which requires cholesterol and dynamin. This initial identification of the endocytic pathway of PDCoV provides a theoretical basis for understanding its pathogenic mechanism and designing new antiviral targets.
Article
Virology
Hongxun Chen, Fei Yu, Jiehua Xu, Shuxin Li, Xiaodong Zhang, Lihui Meng, Kai Hao, Zhe Zhao
Summary: The study found that channel catfish virus (CCV) invades host cells via a low-pH-dependent endocytic pathway, mediated by clathrin. Inhibiting endosomal acidification and clathrin-mediated endocytosis can effectively block CCV infection in host cells.
Article
Microbiology
Shuying Chen, Fan Yang, Zixiang Zhu, Weijun Cao, Kaiqi Lian, Wei Zhang, Zhijian Zhu, Jijun He, Jianhong Guo, Xiangtao Liu, Bin Zhou, Haixue Zheng
Summary: The study reveals that foot-and-mouth disease virus can enter cells through endocytosis and endosomal pathways, even in the absence of integrin and heparan sulfate receptors. Inhibition of endocytosis can block viral internalization, while the acidic environment and the presence of dynamin are crucial for infection.
VETERINARY MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Annamaria Letoha, Anett Hudak, Tamas Letoha
Summary: SARS-CoV-2 variants, particularly Delta and Omicron, exhibit differential cellular uptake mechanisms, with Omicron relying primarily on HSPGs for entry. Delta variant benefits from SDC1, 2, and 4 overexpression, while SDC4 has the most prominent effect on Omicron internalization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Xin Chen, Jun Zheng, Chuanxia Liu, Tingting Li, Xiao Wang, Xuewen Li, Miaofei Bao, Jiangnan Li, Li Huang, Zhaoxia Zhang, Zhigao Bu, Changjiang Weng
Summary: African swine fever (ASF) is a highly contagious viral disease that has caused economic losses in the pig production industry. The mechanism of ASF virus (ASFV) entry into host cells is not fully understood. This study revealed that the host protein CD1d acts as a factor for ASFV entry, and its interaction with p72 facilitates viral infection. CD1d mediates viral entry through clathrin-mediated endocytosis and interacts with EPS15 to facilitate ASFV internalization. These findings highlight CD1d as a potential target for antiviral interventions.
EMERGING MICROBES & INFECTIONS
(2023)
Review
Immunology
Krishnaraju Madavaraju, Raghuram Koganti, Ipsita Volety, Tejabhiram Yadavalli, Deepak Shukla
Summary: Herpes simplex virus has a wide host range and can cause severe infections in humans. Its surface glycoproteins play a crucial role in binding to host cell receptors and facilitating the entry process. Understanding the mechanisms of viral entry is essential for developing antiviral strategies against HSV.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Lina S. Silva-Bermudez, Tatyana N. Sevastyanova, Christina Schmuttermaier, Carolina De La Torre, Leonie Schumacher, Harald Klueter, Julia Kzhyshkowska
Summary: Titanium nanoparticles (TiNPs) have a dual effect on the GDF-15/stabilin-1 interaction in macrophage system, by increasing the production of GDF-15 and suppressing stabilin-1-mediated clearance function. This can lead to unbalanced pro-fibrotic reactions and implant complications.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Chia-Chyi Liu, Suh-Chin Wu, Shang-Rung Wu, Hsiao-Yu Lin, Meng-Shin Guo, Alan Yung-Chih Hu, Yen-Hung Chow, Jen-Ron Chiang, Dar-Bin Shieh, Pele Chong
Article
Virology
Hsiao-Yun Shao, Ying-Chin Chen, Nai-Hsiang Chung, Yi-Ju Lu, Ching-Kun Chang, Shu-Ling Yu, Chia-Chyi Liu, Yen-Hung Chow
Correction
Multidisciplinary Sciences
Ching-Kun Chang, Shang-Rung Wu, Ying-Chin Chen, Kuen-Jin Lee, Nai-Hsiang Chung, Yi-Ju Lu, Shu-Ling Yu, Chia-Chyi Liu, Yen-Hung Chow
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Ching-Kun Chang, Shang-Rung Wu, Ying-Chin Chen, Kuen-Jin Lee, Nai-Hsiang Chung, Yi-Ju Lu, Shu-Ling Yu, Chia-Chyi Liu, Yen-Hung Chow
SCIENTIFIC REPORTS
(2018)
Article
Biotechnology & Applied Microbiology
Ting-Hsuan Chen, Wen-Chun Liu, Chia-Ying Lin, Chia-Chyi Liu, Jia-Tsrong Jan, Maureen Spearman, Michael Butler, Suh-Chin Wu
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Article
Multidisciplinary Sciences
Teik Hwa Ong, Ebenezer Chitra, Srinivasan Ramamurthy, Catherine Chong Sze Ling, Stephen Periathamby Ambu, Fabian Davamani
Article
Pharmacology & Pharmacy
Jo-Yu Hong, Ting-Hsuan Chen, Yu-Jou Chen, Chia-Chyi Liu, Jia-Tsrong Jan, Suh-Chin Wu
ANTIVIRAL RESEARCH
(2019)
Article
Immunology
Ting-Hsuan Chen, Wen-Chun Liu, I-Chen Chen, Chia-Chyi Liu, Ming-Hsi Huang, Jia-Tsrong Jan, Suh-Chin Wu
Review
Pharmacology & Pharmacy
Chih-Yeu Fang, Chia-Chyi Liu
Summary: Hand, foot, and mouth disease poses a serious threat to young children in the Asia-Pacific region, with enterovirus A71 (EV-A71) being a major cause of severe complications. While vaccines exist to prevent EV-A71 infection, there is a lack of effective treatments for other enteroviruses. Novel strategies are needed to develop new therapies for emerging enteroviruses associated with HFMD, including the use of DNA/RNA vaccine technology, structure biology, neutralization antibody studies, and nucleotide aptamer library screening.
EXPERT OPINION ON DRUG DISCOVERY
(2022)
Article
Cell Biology
Rizdwan Rahman, Alyssa Danielle Fonseka, Shiang-Chia Sua, Munirah Ahmad, Ramkumar Rajendran, Stephen Ambu, Fabian Davamani, Alan Soo-Beng Khoo, Ebenezer Chitra
Summary: LfcinB, a natural peptide derived from bovine lactoferrin, exhibits anti-cancer properties against different categories of breast cancer cell lines in vitro and suppresses tumor formation in a mouse xenograft model. This natural peptide also inhibits the invasion of breast cancer cell lines and shows potential as a safe candidate for breast cancer treatment.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Chi-Hsun Chen, Chung-Chu Chen, Wei-Bo Wang, Vania Lionel, Chia-Chyi Liu, Li-Min Huang, Suh-Chin Wu
Summary: In this study, a recombinant ZIKV envelope DIII protein genetically fused with Salmonella typhimurium flagellin was developed as a mucosal antigen. Intranasal immunization with this antigen elicited strong protective immunity against ZIKV challenges, demonstrating its potential for developing mucosal vaccines.
Article
Biology
Seii Ohka, Soon Hao Tan, Eri Ishiyama, Katsutoshi Ogasawara, Tomohito Hanasaka, Kinji Ishida, Kyoji Hagiwara, Chia-Chyi Liu, Pele Choi-Sing Chong, Ken-ichi Hanaki, Giampietro Schiavo
Summary: Recent research has shown that EV71, a causative agent of hand-foot-and-mouth disease, undergoes efficient uncoating in late endosomes containing hSCARB2, M6PR, RAB9, bis(monoacylglycero)phosphate, and LAMP2. The autophagic pathway does not play a critical role in EV71 uncoating, and cation-dependent M6PR is likely to be important for this process.
Article
Biochemical Research Methods
Sheng-Chieh Lien, Chia-Chun Lu, Yu-Sheng Shen, Ya-Ting Yang, Shang-Rung Wu, Chih-Yeu Fang, Yen -Hung Chow, Ching -Len Liao, Jen -Ron Chiang, Chia-Chyi Liu
Summary: This study aimed to develop an approach to isolate and purify highly immunogenic mature EV-A71 particles. By applying a step gradient elution procedure, VP0-cleaved particles of EV-A71 were successfully isolated using Q-membrane anion-exchange chromatography. The purified VP0-cleaved particles exhibited characteristics equivalent to highly infectious F-particles of EV-A71.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Meeting Abstract
Pharmacology & Pharmacy
S. Tan, R. Rajendran, S. Bhattamisra, P. Krishnappa, F. Davamani, E. Chitra, S. Ambu, B. L. Furman, M. Candasamy
BRITISH JOURNAL OF PHARMACOLOGY
(2019)
Article
Chemistry, Inorganic & Nuclear
Khei Yan Lee, Yi Ling Ng, Wai San Wang, Pei Ying Ng, Cheang Wei Chan, Jing Wei Lai, Fabian Davamani, Ebenezer Chitra, Wei Meng Lim, Rakesh Ganguly, Mohd Jamil Maah, Foo Win Yip, Chew Hee Ng
DALTON TRANSACTIONS
(2019)