Article
Public, Environmental & Occupational Health
Alessandro Manenti, Niccolo Solfanelli, Paolo Cantaloni, Livia Mazzini, Margherita Leonardi, Linda Benincasa, Giulia Piccini, Serena Marchi, Martina Boncioli, Chiara Spertilli Raffaelli, Danilo Tacconi, Giada Mattiuzzo, Otfried Kistner, Emanuele Montomoli, Claudia Maria Trombetta
Summary: This study aimed to evaluate a possible approach to performing Monkeypox and vaccinia live-virus microneutralization assays. The results confirmed the presence and cross-reactivity of antibodies elicited by vaccinia-based vaccines, which were able to neutralize the Monkeypox virus in the presence of an external source of complement.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Microbiology
Madina Rasulova, Thomas Vercruysse, Jasmine Paulissen, Catherina Coun, Vanessa Suin, Leo Heyndrickx, Ji Ma, Katrien Geerts, Jolien Timmermans, Niraj Mishra, Li-Hsin Li, Dieudonne Buh Kum, Lotte Coelmont, Steven Van Gucht, Hadi Karimzadeh, Julia Thorn-Seshold, Simon Rothenfusser, Kevin K. Arien, Johan Neyts, Kai Dallmeier, Hendrik Jan Thibaut
Summary: Fast and accurate detection of neutralizing antibodies (nAbs) against yellow fever virus (YFV) is crucial for yellow fever serodiagnosis, outbreak surveillance, and monitoring of vaccine efficacy. The classical plaque reduction neutralization test (PRNT) remains the gold standard for measuring YFV nAbs, but it has limitations in throughput and labor intensity. In this study, we introduce a novel fluorescence-based serum neutralization test (SNTFLUO) with equally high sensitivity and specificity, suitable for high-throughput testing and potential clinical use. Additionally, we present SNTFLUO assays for Japanese encephalitis, Zika, and dengue viruses, offering new possibilities for differential diagnostics.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Arlen-Celina Luecke, Anja vom Hemdt, Janett Wieseler, Carlo Fischer, Marie Feldmann, Simon Rothenfusser, Jan Felix Drexler, Beate Mareike Kuemmerer
Summary: A neutralization test based on wild-type single-round infectious virus replicon particles (VRPs) was established for high-throughput analysis of neutralizing flavivirus antibodies.
Article
Multidisciplinary Sciences
Andrew P. Hederman, Harini Natarajan, Leo Heyndrickx, Kevin K. Arien, Joshua A. Wiener, Peter F. Wright, Evan M. Bloch, Aaron A. R. Tobian, Andrew D. Redd, Joel N. Blankson, Amihai Rottenstreich, Gila Zarbiv, Dana Wolf, Tessa Goetghebuer, Arnaud Marchant, Margaret E. Ackerman
Summary: This study investigates the breadth and potency of antibodies after infection and vaccination. The findings reveal that variants associated with vaccination have significantly greater functional antibody breadth and potency compared to those associated with immunologically vulnerable populations. However, convalescent individuals exhibit higher antibody functional activity against the endemic coronavirus OC43, indicating recognition differences between close and distant human coronavirus strains associated with exposure history.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Nicolas Berthet, Stephane Descorps-Declere, Camille Besombes, Manon Curaudeau, Andriniaina Andy Nkili Meyong, Benjamin Selekon, Ingrid Labouba, Ella Cyrielle Gonofio, Rita Sem Ouilibona, Huguette Dorine Simo Tchetgna, Maxence Feher, Arnaud Fontanet, Mirdad Kazanji, Jean-Claude Manuguerra, Alexandre Hassanin, Antoine Gessain, Emmanuel Nakoune
Summary: The study aimed to sequence the full genome of 10 MPXV isolates collected during CAR epidemics from 2001 to 2018 to determine their phylogenetic relationships among previously described MPXV lineages in Central Africa. The results showed that the 10 CAR isolates belong to lineages closely related to those found in the DRC, suggesting transmissions from wild animals living in the rainforest. This highlights the importance of understanding the socio-economic situation, armed conflicts, and ecological disturbances in CAR, which may increase the risk of zoonotic spillover.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Stephen R. Welch, Jessica R. Spengler, Sarah C. Genzer, JoAnn D. Coleman-McCray, Jessica R. Harmon, Teresa E. Sorvillo, Florine E. M. Scholte, Sergio E. Rodriguez, T. Justin O'Neal, Jana M. Ritter, Georgia Ficarra, Katherine A. Davies, Markus H. Kainulainen, Elif Karaaslan, Eric Bergeron, Cynthia S. Goldsmith, Michael K. Lo, Stuart T. Nichol, Joel M. Montgomery, Christina F. Spiropoulou
Summary: The study demonstrates the clinical impact of a novel NiV-derived nonspreading replicon particle as a highly efficacious and safe vaccine candidate against lethal Nipah disease.
Article
Multidisciplinary Sciences
Jeffrey L. Americo, Catherine A. Cotter, Patricia L. Earl, Ruikang Liu, Bernard Moss
Summary: Intranasal administration of a SARS-CoV-2 vaccine induces stronger immune responses compared to intramuscular administration, with higher levels of antibodies and specific T cells. Additionally, intranasal vaccination can prevent or rapidly eliminate SARS-CoV-2 infection.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Infectious Diseases
Petra Fay, Georgina Limon, Gerelmaa Ulziibat, Buyantogtokh Khanui, Odonchimeg Myagmarsuren, Gessica Tore, Bodisaikhan Khishgee, John Flannery, Batkhuyag Sandag, Batchuluun Damdinjav, Philippa M. Beard
Summary: This study used a commercial ELISA kit to examine the humoral response of sheep vaccinated with a live-attenuated CPPV strain in Mongolia. The results showed that the commercial CPPV ELISA kit is a robust and reliable assay for post-vaccination surveillance in resource-restricted settings and provide temporal parameters for planning sheeppox monitoring programmes.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Immunology
Thomas L. Rudge Jr, Nicholas J. Machesky, Karen A. Sankovich, Erin E. Lemmon, Christopher S. Badorrek, Rachel Overman, Nancy A. Niemuth, Michael S. Anderson
Summary: Since the discovery of Marburg virus in 1967 and Ebola virus in 1976, there have been over 40 reported outbreaks of filovirus disease with case fatality rates greater than 50%. The need for efficacious vaccines against these highly pathogenic filoviruses is highlighted, with the development of vaccines needing to be vetted through regulatory pathways such as the U.S. FDA Animal Rule. There is currently no identified correlate of protection for filovirus disease, but anti-glycoprotein antibodies are believed to play a critical role in protection and are thus a focus for vaccine development.
Article
Virology
Elena Criscuolo, Benedetta Giuliani, Roberto Ferrarese, Davide Ferrari, Massimo Locatelli, Massimo Clementi, Nicasio Mancini, Nicola Clementi
Summary: Since May 2022, there have been reported cases of monkeypox virus (MPXV) infections in non-endemic countries, leading to the most severe outbreak described so far. Despite genetic variations in the current outbreak's isolated viruses, serum antibodies produced by first-generation smallpox vaccines have shown the ability to neutralize the current MPXV more than 40 years after vaccination.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Immunology
Xiaoling Cao, Junjie Zai, Qingzhen Zhao, Lilan Xie, Yaoming Li
Summary: This study demonstrated that intranasal immunization with a recombinant Vaccinia virus expressing the trimeric receptor-binding domain of SARS-CoV-2 spike protein induced strong humoral and cellular immune responses in mice and rabbits. The immunized animals developed high levels of neutralizing IgG and IgA in their sera, as well as RBD-specific IFN-gamma-secreting lymphocytes. Notably, secretory IgA in nasal washes and bronchoalveolar lavage fluids showed substantial neutralization activities. These findings suggest that the recombinant VV expressing trimeric RBD has the potential to be developed as a mucosal vaccine.
Article
Oncology
Quinn T. Storozynsky, Kate C. Agopsowicz, Ryan S. Noyce, Amirali B. Bukhari, Xuefei Han, Natalie Snyder, Brittany A. Umer, Armin M. Gamper, Roseline Godbout, David H. Evans, Mary M. Hitt
Summary: Glioblastoma (GB) is an incurable brain cancer with immune suppression. Radiotherapy is commonly used, but it cannot completely eliminate GB cells due to radioresistance. In this study, researchers found that combining radiation with the oncolytic AF4LAJ2R vaccinia virus (VACV) had significantly superior anticancer effects compared to monotherapy in mouse models. The combination therapy also increased the ratio of CD8+ effector T cells to regulatory T cells. This study validates the use of radiation with an oncolytic AF4LAJ2R VACV to improve the treatment of GB.
Article
Biochemical Research Methods
Krongkan Saipin, Butsaya Thaisomboonsuk, Bunpote Siridechadilok, Nithinart Chaitaveep, Pongrama Ramasoota, Chunya Puttikhunt, Sutha Sangiambut, Anthony Jones, Romchat Kraivong, Rungtawan Sriburi, Poonsook Keelapang, Nopporn Sittisombut, Jiraphan Junjhon
Summary: This study aimed to generate a replication competent luciferase-secreting DENV reporter (LucDENV2) and evaluate its feasibility in neutralizing and infection-enhancing antibody assays in comparison with wild-type DENV2. The results showed that LucDENV2 replicated to similarly high levels as that of the parent 16681 virus and exhibited neutralization patterns similar to LucSIP and wild-type DENV2, suggesting comparable antigenic properties. This high-titer, replication competent, luciferase-secreting DENV reporter could serve as a useful tool for quantification of neutralizing and infection-enhancing antibodies in DENV-endemic areas.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Immunology
Lilan Xie, Yaoming Li
Summary: This review focuses on the advances in VV-based vaccine vector studies, including construction approaches, the impact of pre-existing immunity, and antigen-specific immune responses. The specific discussion is on recombinant VV-based flaviviruses.
Article
Immunology
Vladimir A. A. Gushchin, Darya A. A. Ogarkova, Inna V. V. Dolzhikova, Olga V. V. Zubkova, Igor V. V. Grigoriev, Andrei A. A. Pochtovyi, Anna A. A. Iliukhina, Tatiana A. A. Ozharovskaia, Nadezhda A. A. Kuznetsova, Daria D. D. Kustova, Artem Y. Y. Shelkov, Denis I. I. Zrelkin, Alina S. S. Odintsova, Daria M. M. Grousova, Vladislav Y. Y. Kan, Sona A. A. Davtyan, Andrei E. E. Siniavin, Elizaveta D. D. Belyaeva, Andrei G. G. Botikov, Arina A. A. Bessonova, Lyudmila A. A. Vasilchenko, Daria V. V. Vasina, Denis A. A. Kleymenov, Egor A. A. Slutskiy, Artem P. P. Tkachuk, Olga A. A. Burgasova, Svetlana Y. Y. Loginova, Evgeny V. V. Rozhdestvensky, Dmitry V. V. Shcheblyakov, Alexander N. N. Tsibin, Andrey G. G. Komarov, Vladimir I. I. Zlobin, Sergel V. V. Borisevich, Boris S. S. Naroditsky, Denis Y. Y. Logunov, Alexander L. L. Gintsburg
Summary: The study focused on the residual immunity among Moscow residents, indicating the need for priority vaccination in individuals aged 45 and under and 66 and over.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Yunlong Cao, Jing Wang, Fanchong Jian, Tianhe Xiao, Weiliang Song, Ayijiang Yisimayi, Weijin Huang, Qianqian Li, Peng Wang, Ran An, Yao Wang, Xiao Niu, Sijie Yang, Hui Liang, Haiyan Sun, Tao Li, Yuanling Yu, Qianqian Cui, Shuo Liu, Xiaodong Yang, Shuo Du, Zhiying Zhang, Xiaohua Hao, Fei Shao, Ronghua Jin, Xiangxi Wang, Junyu Xiao, Youchun Wang, Xiaoliang Sunney Xie
Summary: The Omicron variant of SARS-CoV-2 contains 15 mutations in the receptor-binding domain, leading to evasion of over 85% of tested neutralizing antibodies. Different epitope groups of neutralizing antibodies are affected to varying degrees by single mutations of Omicron. Antibodies targeting the conserved region of sarbecovirus remain most effective against Omicron.
Article
Multidisciplinary Sciences
Bo Yang, Yuanyuan Jia, Yumin Meng, Ying Xue, Kefang Liu, Yan Li, Shichao Liu, Xiaoxiong Li, Kaige Cui, Lina Shang, Tianyou Cheng, Zhichao Zhang, Yingxiang Hou, Xiaozhu Yang, Hong Yan, Liqiang Duan, Zhou Tong, Changxin Wu, Zhida Liu, Shan Gao, Shu Zhuo, Weijin Huang, George Fu Gao, Jianxun Qi, Guijun Shang
Summary: This study reveals the critical role of SNX27 in regulating ACE2 receptor homeostasis by preventing the entry of ACE2/virus complex into lysosome/late endosome through its interaction with the retromer complex. This mechanism serves as a countermeasure against invasion by ACE2 receptor-utilizing SARS coronaviruses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Virology
Chunnan Liang, Chenyan Zhao, Tianlong Liu, Bo Liu, Zhiguo Liu, Huili Huang, Wei Liu, Minghai Zhao, Nan Xu, Qiong Lu, Jianhui Nie, Li Zhang, Weijin Huang, Ruiping She, Youchun Wang
Summary: We characterized the course of chronic HEV infection in immunocompetent rabbits and found that 47.5% of the rabbits developed chronic infection with persistent viral shedding for more than 6 months.
Article
Immunology
Xingxing Li, Ling Wang, Jingjing Liu, Enyue Fang, Xiaohui Liu, Qinhua Peng, Zelun Zhang, Miao Li, Xinyu Liu, Xiaohong Wu, Danhua Zhao, Lihong Yang, Jia Li, Shouchun Cao, Yanqiu Huang, Leitai Shi, Hongshan Xu, Yunpeng Wang, Yue Suo, Guangzhi Yue, Jianhui Nie, Weijin Huang, Wenjuan Li, Yuhua Li
Summary: Combining intramuscular and intranasal vaccination routes can result in more potent immune responses. In a mouse study, a prime-boost protocol with intramuscular priming and intranasal boosting showed the highest levels of antibodies and T-cell responses. This study provides important reference data for assessing adenovirus-based COVID-19 vaccination schemes.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Virology
Fan Zhang, Zhaogeng Yang, Cong Dai, Qiyu He, Zhaochao Liang, Tianxu Liu, Weijin Huang, Youchun Wang, Lin Wang, Ling Wang
Summary: This study demonstrated that the HEV vaccine could induce high titers of protective antibodies against HEV in pregnant rabbits within a short period of time, and these antibodies could be transferred to neonates through the placenta and breast milk, providing protection against HEV infection. This suggests that HEV 239 vaccine could be considered for maternal vaccination.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Virology
Xinjie Li, Yue Yin, Qianqian Cui, Weijin Huang, Qinghua Zou, Tao Shen
Summary: The long-term protective efficacy of neutralizing antibodies (Nabs) against Omicron subvariants after inactivated booster vaccines remains uncertain. This study found that the antibody response against Omicron subvariants was weaker compared to D614G, with BA.4/5 being the least responsive. However, the antibody potency post-booster vaccination was sustained and measurable.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Immunology
Jingjing Liu, Xishan Lu, Xingxing Li, Weijin Huang, Enyue Fang, Wenjuan Li, Xiaohui Liu, Minglei Liu, Jia Li, Ming Li, Zelun Zhang, Haifeng Song, Bo Ying, Yuhua Li
Summary: This study developed an mRNA vaccine using the nucleotide sequence encoding structural proteins of CHIKV, and evaluated its immunogenicity. The encoded proteins elicited high levels of neutralizing antibody titers and T cell-mediated cellular immune responses in mice. Furthermore, a homologous booster mRNA vaccine regimen using different immunization strategies showed higher levels of neutralizing antibody titers and T-cell immune responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Yufeng Luo, Shuo Liu, Jiguo Xue, Ye Yang, Junxuan Zhao, Ying Sun, Bolun Wang, Shenyi Yin, Juan Li, Yuchao Xia, Feixiang Ge, Jiqiao Dong, Lvze Guo, Buqing Ye, Weijin Huang, Youchun Wang, Jianzhong Jeff Xi
Summary: This study established a robust mammalian cell-surface-display platform to study the interactions between SARS-CoV-2 variants, cellular receptor ACE2, and monoclonal antibodies on a large scale. By analyzing the mutational landscape, it was found that certain key mutations in the spike protein can increase infectivity and confer resistance to specific monoclonal antibodies. These methods have significant implications for the precise control of SARS-CoV-2 in the future.
Article
Immunology
Xi Wu, Nana Fang, Ziteng Liang, Jianhui Nie, Sen Lang, Changfa Fan, Chunnan Liang, Weijin Huang, Youchun Wang
Summary: Researchers have developed an animal model using SARS-CoV-2 pseudovirus, which is administered intranasally for infection, and bioluminescent imaging allows monitoring of infected tissues and viral load. The model can be used to evaluate the protective efficacy of vaccines and antiviral drugs, as well as the tissue tropism of different viral variants.
Article
Infectious Diseases
Feng-Cai Zhu, Guo-Hua Zhong, Wei-Jin Huang, Kai Chu, Li Zhang, Zhao-Feng Bi, Kong-Xin Zhu, Qi Chen, Ting-Quan Zheng, Ming-Lei Zhang, Sheng Liu, Jin-Bo Xu, Hong-Xing Pan, Guang Sun, Feng-Zhu Zheng, Qiu-Fen Zhang, Xiu-Mei Yi, Si-Jie Zhuang, Shou-Jie Huang, Hui-Rong Pan, Ying-Ying Su, Ting Wu, Jun Zhang, Ning-Shao Xia
Summary: Cecolin 9, an Escherichia coli-produced HPV 9-valent vaccine, was found to be non-inferior in terms of immunogenicity compared to Gardasil 9. It has the potential to contribute to the global accessibility of 9-valent HPV vaccinations, particularly in low-income and middle-income countries.
LANCET INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Yue-Mei Hu, Zhao-Feng Bi, Ya Zheng, Li Zhang, Feng-Zhu Zheng, Kai Chu, Ya-Fei Li, Qi Chen, Jia-Li Quan, Xiao-Wen Hu, Xing-Cheng Huang, Kong-Xin Zhu, Ya-Hui Wang-Jiang, Han -Min Jiang, Xia Zang, Dong-Lin Liu, Chang -Lin Yang, Hong-Xing Pan, Qiu-Fen Zhang, Ying-Ying Su, Shou-Jie Huang, Guang Sun, Wei-Jin Huang, Yue Huang, Ting Wu, Jun Zhang, Ning-Shao Xia
Summary: This study evaluated the immunogenicity and safety of the Escherichia coli-produced second-generation nonavalent HPV vaccine (Cecolin 9). The results showed that Cecolin 9 had good immunogenicity against all nine HPV types and was well tolerated.
Article
Biochemistry & Molecular Biology
Zhen Cui, Wenxin Luo, Ruihong Chen, Yalun Li, Zhoufeng Wang, Yong Liu, Shuo Liu, LeiLei Feng, Zijing Jia, Ruixin Cheng, Jun Tang, Weijin Huang, Yanjun Zhang, Huawen Liu, Xiangxi Wang, Weimin Li
Summary: The emergence of adapted variants of the SARS-CoV-2 virus has led to an increase in breakthrough infections worldwide. Analysis of immune responses in vaccinated individuals reveals limited resistance to Omicron in those with no prior infection but significant immunity in those previously infected. T-cell responses remain largely unaffected, indicating the importance of T-cell-mediated cellular immunity. Administration of a third vaccine dose enhances neutralizing antibodies and memory B cells, improving protection against emerging variants.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Ye Sang, Zhen Zhang, Fan Liu, Haitao Lu, Changxiao Yu, Huisheng Sun, Jinrong Long, Yiming Cao, Jierui Mai, Yiqi Miao, Xin Wang, Jiaxin Fang, Youchun Wang, Weijin Huang, Jing Yang, Shengqi Wang
Summary: Monkeypox has been declared a public health emergency by the World Health Organization. Two mRNA vaccines, mRNA-A-LNP and mRNA-B-LNP, based on specific proteins of the monkeypox virus, have been developed and shown to induce immune responses in mice, including specific antibodies and cellular immunity. These vaccines also provide protection against vaccinia virus challenge in mice and have the potential to be used against other orthopoxviruses.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Correction
Cell Biology
Shuo Liu, Zijing Jia, Jianhui Nie, Ziteng Liang, Jingshu Xie, Lei Wang, Li Zhang, Xiangxi Wang, Youchun Wang, Weijin Huang
Article
Biochemistry & Molecular Biology
Rongzhang He, Xingyu Zheng, Jian Zhang, Bo Liu, Qijie Wang, Qian Wu, Ziyan Liu, Fangfang Chang, Yabin Hu, Ting Xie, Yongchen Liu, Jun Chen, Jing Yang, Shishan Teng, Rui Lu, Dong Pan, You Wang, Liting Peng, Weijin Huang, Velislava Terzieva, Wenpei Liu, Youchun Wang, Yi-Ping Li, Xiaowang Qu
Summary: The study found that spike-specific CXCR3(+) T-FH cells play a crucial role in antibody maintenance and recall responses after SARS-CoV-2 infection and vaccination, exhibiting a more durable response and better ability to induce antibody secretion.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
