Article
Chemistry, Multidisciplinary
Mathilde Briday, Francois Halle, Lauriane Lecoq, Sylvie Radix, Juliette Martin, Roland Montserret, Marie Dujardin, Marie-Laure Fogeron, Michael Nassal, Beat H. Meier, Thierry Lomberget, Anja Bockmann
Summary: Hepatitis B virus (HBV) is a significant human pathogen and its capsid-forming core protein (Cp) plays a key role in envelopment. Recent studies have found that Triton X-100, a detergent used in Cp purification, strongly binds to the hydrophobic pocket of Cp. The aromatic moiety is crucial for binding, while the aliphatic chain affects binding affinity. Moreover, the hydrophilic tail shows high tolerance to changes in length and type.
Article
Virology
Haohai Huang, Dan Liao, Guanghui Zhou, Bin He, Rong Pu, Yejia Cui
Summary: This study investigated the effect of miR-194-3p on autophagy after RV infection and its regulation mechanism of RV replication. The results demonstrated that upregulation of miR-194-3p or repression of SIRT1 inhibited RV replication through the regulation of autophagy. In vivo experiments confirmed that upregulation of miR-194-3p or downregulation of SIRT1 also suppressed RV replication.
Article
Biochemistry & Molecular Biology
Ana Luisa De Sousa-Coelho, Mar Gacias, Brian T. O'Neill, Joana Relat, Wolfgang Link, Diego Haro, Pedro F. Marrero
Summary: The study found that FOXO1 is a protein that interacts with PPARa and can inhibit its activity on the FGF21 gene promoter, thereby regulating FGF21 expression. This suggests that FOXO1 is an important factor in the regulation of FGF21.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Miao Yang, Xiaomei Ling, Jinfang Xiao
Summary: This study suggests that miR-141 is involved in lung ischemia-reperfusion injury by regulating EGFR, beta-catenin, and autophagy. It provides a potential therapeutic target for the treatment of lung ischemia-reperfusion injury.
Article
Toxicology
Bo Song, Xin-Xiang Wang, Hai-Yan Yang, Ling-Ting Kong, Hong-Yan Sun
Summary: The study discussed the potential effects of miR-141 in sepsis-induced cardiomyopathy by targeting DAPK1. Results showed that miR-141 could decrease inflammatory response and reduce myocardial cell apoptosis, playing a promising protective role in SIC.
HUMAN & EXPERIMENTAL TOXICOLOGY
(2021)
Article
Cell Biology
Hyunjung Lee, Young In Kim, Farida S. Nirmala, Ji-Sun Kim, Hyo-Deok Seo, Tae Youl Ha, Young-Jin Jang, Chang Hwa Jung, Jiyun Ahn
Summary: Post-menopausal conditions exacerbate the biological aging process, leading to visceral adiposity and sarcopenia. Mitochondrial impairment, along with the upregulation of miR-141-3p and subsequent downregulation of Fkbp5 and Fibin, plays a role in the development of obesogenic sarcopenia. Inhibiting miR-141-3p could be a potential therapeutic target for alleviating this condition.
Article
Pharmacology & Pharmacy
Yuan-Yuan Luo, Ke-Gong Tao, Yi-Ting Lu, Bin-Bin Li, Kai-Ming Wu, Chen-Hong Ding, Fang-Zhi Yan, Yue Liu, Yong Lin, Xin Zhang, Xin Zeng
Summary: This study identified hsa_circ_0098181 as a potential therapeutic target for hepatocellular carcinoma (HCC). The downregulation of hsa_circ_0098181 was associated with poor prognosis in HCC patients. Hsa_circ_0098181 sponge miR-18a-3p to regulate PPARA, inhibiting the proliferation, invasion, migration, and colony formation of HCC cells.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Environmental Sciences
Xin Xin, Lian Duan, Huimin Yang, Hang Yu, Yandong Bao, Dalin Jia, Nan Wu, Ying Qiao
Summary: miR-141-3p is significantly upregulated in cardiomyocytes of obese rats induced by a high-fat diet, but its role in myocardial lipotoxicity remains unclear. The study shows that miR-141-3p regulates saturated fatty acid-induced cardiomyocyte apoptosis through targeting PSEN1 and inhibiting the Notch1/PTEN/AKT pathway.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Oncology
Shuying Chen, Jingjun Zhang, Qiudan Chen, Juan Cheng, Xiaotong Chen, Yinqi Mao, Wei Chen, Chenbin Liu, Han Wu, Yuan Lv, Yong Lin
Summary: miR-141 and miR-200a are significantly downregulated in liver cancer and are closely associated with clinical features. They synergistically inhibit the expression of E-cadherin and vimentin during epithelial-mesenchymal transition by targeting STAT4, influencing the proliferation, migration, and invasion of liver cancer cells. Overexpression of both miRNAs together has stronger effects, while overexpression of STAT4 can reverse their tumor suppressive roles. These findings expand the understanding of the tumor suppressor mechanisms of the miR-200 family and may have implications for using miRNAs in the diagnosis, prognosis, and treatment of liver cancer.
Article
Biochemistry & Molecular Biology
Guanqun Dai, Shencun Fang, Hui Kong, Subash C. B. Gopinath, Weiping Xie, Hong Wang
Summary: The study focused on the role of miR-133a-3p in cisplatin resistance in non-small cell lung cancer. Upregulation of miR-133a-3p led to inhibited cell growth and enhanced apoptosis, while lowering resistance to cisplatin. The mechanism involved negative regulation of replication factor C3 and identification of 94 potential target genes for miR-133a-3p.
PROCESS BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Wei Deng, Dan Huang, HongWu Xie, LiMin Wang, Qun Shen, RongRong Zeng, YuanLian Huang, JianHua Li, Bo Yang
Summary: The study found that Danhong injection (DHI) can inhibit the development of diabetic retinopathy and nephropathy by regulating the miR-30d-5p-JAK1 axis, increasing miR-30d-5p levels, and targeting JAK1.
Article
Biochemistry & Molecular Biology
Wei-Shiung Lian, Re-Wen Wu, Yu-Shan Chen, Jih-Yang Ko, Shao-Yu Wang, Holger Jahr, Feng-Sheng Wang
Summary: The study demonstrated that miR-29a attenuated high-fat diet-induced body overweight, hyperglycemia, and hypercholesterolemia by controlling adipose tissue metabolism and bone homeostasis. It also promoted brown/beige adipocyte formation and inhibited white adipocyte markers, providing a potential new target for treating obesity-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Indrajit Saha, Nimisha Ghosh, Dariusz Plewczynski
Summary: SARS-CoV-2 poses a great challenge to mankind due to its evolution and variants. Human miRNA may play a vital role in viral infection and can be used as a therapeutic approach to inhibit viral replication.
Article
Chemistry, Analytical
Xiankang Niu, Changtong Lu, Dan Su, Fang Wang, Weihong Tan, Fengli Qu
Summary: In this study, a photoelectrochemical biosensing platform was developed for ultrasensitive miRNA-141 detection using CdS quantum dots and Tph-2H coated GCE. By combining an enzyme-assisted recycle amplification reaction and a magnetic bead-based separation strategy, the detection sensitivity of miRNA-141 could be significantly enhanced. The proposed biosensor demonstrated efficient elimination of false-positive or false-negative signals and a wide linear response range with a low detection limit for miRNA-141.
ANALYTICAL CHEMISTRY
(2021)
Article
Cell Biology
Poyuan Xing, Yang Dong, Jingyu Zhao, Zhou Zhou, Zhao Li, Yu Wang, Mengfei Li, Xinghua Zhang, Xuefeng Chen
Summary: The study demonstrates that replication stress suppresses resection pathways mediated by Sgs1/Dna2 and Exo1, leading to disruption in DNA replication and repair. Mrc1 plays a crucial role in inhibiting DSB end resection through regulating chromatin compaction to limit access of HR proteins.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Virology
Yang Yang, Peng Yang, Cong Huang, Yuming Wu, Zhe Zhou, Xuejun Wang, Shengqi Wang
Summary: The study found that neferine effectively inhibits infection by various coronaviruses by blocking host calcium channels, thus showing potential as a promising lead for pan-coronaviruses therapeutic drug development.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hailun Jiang, Yaoliang Zhang, Yuming Wu, Jiawei Cheng, Shasha Feng, Jian Wang, Xuejun Wang, Maosheng Cheng
Summary: Flavivirus, including Dengue Virus (DENV) and Zika virus (ZIKV), infects and kills millions of people every year, but there is no approved treatment available. In this study, we investigated the inhibitory mechanisms of Montelukast, an existing drug, against the replication of DENV and ZIKV. Through computational methods and in vitro experiments, we found that Montelukast can bind to NS2B-NS3 proteases of DENV and ZIKV and act as a competitive inhibitor. This research provides new insights for the development of potential antiviral drugs.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Medicinal
Xincheng Lin, Jiawei Cheng, Yuming Wu, Yaoliang Zhang, Hailun Jiang, Jian Wang, Xuejun Wang, Maosheng Cheng
Summary: In this study, a potential covalent inhibitor of NS2B-NS3 was discovered through chemical modification and showed promising anti-DENV activity. The findings provide valuable insights for drug discovery against dengue virus.
ACS MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Medicine, Research & Experimental
Guifang Du, Xiang Xu, Junting Wang, Xuejun Wang, Yang Ding, Fei Li, Yu Sun, Huan Tao, Yawen Luo, Hao Li, Xiaochen Bo, Hebing Chen
Summary: In this study, the researchers investigated the chromatin accessibility at the promoters of host factor genes in various tissues, tumor types, and cell lines to understand the tropism mechanism of SARS-CoV-2. They found tissue and cell-specific patterns in the accessibility of ACE2 and TMPRSS2 promoters, consistent with previous clinical research. The study also revealed the role of type I interferon in inducing ACE2 expression by enhancing the binding of HNF1A, a transcription factor, to the ACE2 promoter.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Pharmacology & Pharmacy
Yingying Mao, Xuejun Wang, Andrew Li, Ying Li, Hai Huang, Renhe Yan, Yanling Zhang, Jinlong Li, Hongwei Li, Shengqi Wang
Summary: This study identifies a novel treatment approach for chronic hepatitis B by inhibiting HBV replication through RNA interference. The results show that AAV8-amiRNA135 has anti-HBV effects in vivo, and these effects are time and dose-dependent.
ANTIVIRAL RESEARCH
(2022)
Letter
Infectious Diseases
Lin Yao, Ka-Li Zhu, Xiao-Lin Jiang, Xue-Jun Wang, Bing-Dong Zhan, Hui-Xia Gao, Xing-Yi Geng, Li-Jun Duan, Er-Hei Dai, Mai-Juan Ma
LANCET INFECTIOUS DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
Yuechao Zhao, Tan Zhang, Xuelian Shen, Aixue Huang, Hui Li, Lin Wang, Xuemei Liu, Xuejun Wang, Xiang Song, Shengqi Wang, Jie Dong, Ningsheng Shao
Summary: This study demonstrates the specific binding of TNF-a to certain inflammation-related microRNAs and its delivery into recipient cells through TNF-a receptors, highlighting the potential of TNF-a as a delivery system for therapeutic microRNAs.
Article
Biochemistry & Molecular Biology
Jiawei Cheng, Shasha Feng, Yaoliang Zhang, Tong Ding, Hailun Jiang, Zhigang Zhang, Jian Wang, Xuejun Wang, Maosheng Cheng
Summary: Dengue virus (DENV) is the most widespread arbovirus in the world. Potent NS2B-NS3 covalent inhibitors with increased enzymatic activities and no cellular toxicity have been discovered. Comprehensive structure-activity relationship analysis provides informative understanding of the binding modes of covalent inhibitors targeting NS2B-NS3.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Immunology
Bi-Nan Lu, Ka -Li Zhu, Xiao-Ming Cui, Lin Yao, Xue-Jun Wang, Guo-Lin Wang, Li-Jun Duan, Aruna Qian, Mai-Juan Ma
Summary: SARS-CoV-1 survivors show potent immune response to SARS-CoV-2 after booster vaccination, including neutralization against Omicron subvariants. Naive healthy individuals have weaker neutralization against Omicron, but exhibit comparable robust T cell responses.
CLINICAL IMMUNOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Qiming Yin, Xiang Song, Peng Yang, Wen Yang, Xinyu Li, Xuejun Wang, Shengqi Wang
Summary: In this study, a new strategy of incorporating glycyrrhizic acid (GA) and polyene phosphatidylcholine (PPC) into lipid nanoparticles (GA/PPC-modified LNPs) was developed, which showed enhanced cellular uptake, improved gene-silencing efficiency, reduced cytotoxicity, and increased siRNA stability. GA/PPC-modified LNPs targeting NF-kappa B attenuated acute liver injury and facilitated intracellular delivery of antisense oligonucleotides (ASOs) and mRNA inhibiting viral infection. These findings suggest that GA/PPC-modified LNPs could serve as a promising nucleic acid delivery system.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2023)
Article
Cell Biology
Ka -Li Zhu, Xiao-Lin Jiang, Bing-Dong Zhan, Xue-Jun Wang, Xian Xia, Guo-Ping Cao, Wen-Kui Sun, Peng-Xiang Huang, Jin-Zhong Zhang, Yu-Ling Gao, Er-Hei Dai, Hui-Xia Gao, Mai-Juan Ma
Summary: A study found that the durability of neutralizing antibodies against the Omicron subvariants is uncertain 4-6 months after vaccination or infection. Two doses of CoronaVac or a third dose of ZF2001 produce limited neutralization against Omicron subvariants 6 months after vaccination. Hybrid immunity and Delta, BA.1, and BA.2 breakthrough infections induce long-term persistence of the antibody response, with over 70% of sera neutralizing BA.1, BA.2, BA.4/BA.5, and BF.7. However, BQ.1.1 and XBB, as well as BA.2.75, show higher resistance to neutralization. These data suggest the need for additional vaccination in CoronaVac or ZF2001-vaccinated individuals and provide insight into the durability of neutralization against Omicron subvariants.
Article
Genetics & Heredity
Xue-Jun Wang, Lin Yao, Hong-Yun Zhang, Ka-Li Zhu, Jing Zhao, Bing-Dong Zhan, Yi-Ke Li, Xue-Juan He, Cong Huang, Zhuang-Ye Wang, Ming-Dong Jiang, Peng Yang, Yang Yang, Guo-Lin Wang, Sheng-Qi Wang, Er-Hei Dai, Hui-Xia Gao, Mai-Juan Ma
Summary: The study shows that Omicron subvariants have significant evasion from neutralizing antibodies induced by BBIBP-CorV vaccine, but a third dose can enhance the neutralizing antibody potency against Delta and Omicron subvariants. Additionally, different subvariants exhibit varying resistance to immunity induced by breakthrough infections.
Letter
Virology
Xuejun Wang, Yang Yang, Zhifei Song, Yiming Wang, Peng Yang, Xinyu Li, Fei Wang, Mingming Wang, Liting Shao, Shengqi Wang
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jianglin Zhou, Xuejun Wang, Zhe Zhou, Shengqi Wang
Summary: The exceptional outbreak of monkeypox in 100 non-endemic countries since 2022 poses a major public health concern. Analysis of codon usage patterns in the 2022 outbreak strains of monkeypox virus revealed unique evolutionary features, with codon usage bias predominantly determined by mutation pressure. The virus also showed adaptation to various primate hosts, including humans, indicating the importance of sustained monitoring.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Letter
Biochemistry & Molecular Biology
Cong Huang, Yang Yang, Peng Yang, Fei Wang, Xinyu Li, Xiang Song, Yiming Wang, Cuiyun Yu, Xuejun Wang, Shengqi Wang
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2022)