Article
Biochemistry & Molecular Biology
Giulia Querio, Susanna Antoniotti, Federica Geddo, Renzo Levi, Maria Pia Gallo
Summary: TMAO may be involved in impairing the endothelial-dependent vasodilatory mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Hannah K. Fandl, Vinicius P. Garcia, John W. Treuth, Lillian M. Brewster, Jared J. Greiner, Kevin P. Davy, Brian L. Stauffer, Christopher A. Desouza
Summary: Obesity and hypertension are associated with increased risk of heart failure, and this study explores the effects of endothelial microvesicles (EMVs) from obese/hypertensive individuals on cardiomyocyte hypertrophy and fibrosis, as well as eNOS expression and NO production.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Chemistry, Physical
Namitha Brijit Bejoy, Prahlad Roy Chowdhury, G. Naresh Patwari
Summary: The release of NO upon photodissociation of nitroaromatic compounds is influenced by the interaction between the NO2 group and substituent in the ortho position. Two distinct NO elimination channels are observed with a bimodal translational energy distribution of the NO photofragment. The hydrogen bonding ability of the ortho substituent regulates the ratio of slow-to-fast branching for NO release, with OH and NH2 favoring the roaming mechanism.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Medicine, Research & Experimental
Jun -Ha Hwang, Woong Heo, Jung Il Park, Kyung Min Kim, Ho Taek Oh, Gi Don Yoo, Jeekeon Park, Somin Shin, Youjin Do, Mi Gyeong Jeong, Eun Sook Hwang, Jeong-Ho Hong
Summary: Endothelial TAZ is involved in the production of nitric oxide (NO) and inhibits damage-induced liver fibrosis. It regulates NO production by transcriptional regulation of Nos3. Loss of TAZ leads to capillarization of liver sinusoidal endothelial cells and a decrease in NO concentration, accelerating liver fibrosis.
Article
Polymer Science
Yang Zhou, Peixuan Wu, Jianfeng Wu, Joshua C. Doverspike, Qi Zhang, Jinyu Shao, Chuanwu Xi, Yuanyuan Liu, Mark E. Meyerhoff
Summary: This study reported the preparation of NO-releasing poly(n-butyl methacrylate) films by incorporating S-nitroso-N-acetylpenicillamine, and investigated the NO-releasing profiles of such films under physiological conditions. The results showed that the PBMA films doped with SNAP exhibited good NO-releasing performance with antimicrobial and anti-biofilm properties against bacteria. This controllable NO release using PBMA films could be beneficial for therapeutic or biomedical device applications.
Article
Biochemistry & Molecular Biology
Divya Seth, Colin T. Stomberski, Precious J. McLaughlin, Richard T. Premont, Kathleen Lundberg, Jonathan S. Stamler
Summary: The aim of this study was to investigate the interactions between nitric oxide synthase (NOS) isoforms and proteins, as well as the mechanisms by which they mediate S-nitrosylation. The results showed that each NOS isoform interacts with and S-nitrosylates many proteins, but a large proportion of S-nitrosylated proteins are not associated with NOS. Additionally, the interactomes and S-nitrosylomes of individual NOS isoforms are largely unique, indicating specific roles for each isoform in S-nitrosylation.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Review
Biochemistry & Molecular Biology
Amir Asgari, Paul Jurasz
Summary: Megakaryocytes are important members of the hematopoietic system, responsible for regulating vascular homeostasis through platelets. The process of megakaryopoiesis generates mature megakaryocytes which release proplatelets into blood vessels. Nitric oxide (NO) plays a modulatory role in hematopoiesis and hemostasis. This review summarizes the effect of NO and its signaling on megakaryopoiesis and thrombopoiesis under physiological and pathophysiological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Tatsiana Suvorava, Sara Metry, Stephanie Pick, Georg Kojda
Summary: Vascular tone regulation by nitric oxide is a crucial physiological function, with endothelial dysfunction potentially being an underlying cause of hypertension. Increasing eNOS activity reduces blood pressure, while decreasing activity has the opposite effect. These findings have important implications for pre-hypertensive treatment.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
S. Contreras-Duarte, C. Cantin, M. Farias, A. Leiva
Summary: This study evaluated the effects of MSPD on GDM and how these pathologies together affect fetoplacental endothelial function. Results showed increased TC and TG in MSPD and GDM-MSPD groups, decreased eNOS activity, increased hCat1 protein and L-arginine transport, and higher Arginase II protein and activity in GDM-MSPD compared to MPD.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Maxwell Mathias, Joann Taylor, Elizabeth Mendralla, Marta Perez
Summary: The study on bronchopulmonary dysplasia (BPD) in a mouse model found that SOD3 knockout (KO) mice exhibited better survival and less alveolar and microvascular remodeling under chronic hyperoxia exposure. Additionally, SOD3 KO mice showed increased total SOD activity and VEGF expression compared to wild-type mice.
Article
Medicine, Research & Experimental
Shupeng Hu, Qiangzhong Pi, Xiudan Xu, Jianghong Yan, Yongzheng Guo, Wanying Tan, An He, Zhe Cheng, Suxin Luo, Yong Xia
Summary: This study investigates the role of eNOS in vascular dysfunction induced by sepsis. Increased phosphorylation of eNOS in early sepsis leads to decreased vasodilation, while decreased levels of eNOS in late sepsis result in diminished nitric oxide-dependent relaxation responses.
Article
Biochemistry & Molecular Biology
Shinya Yokomizo, Malte Roessing, Atsuyo Morita, Timo Kopp, Emiyu Ogawa, Wataru Katagiri, Susanne Feil, Paul L. Huang, Dmitriy N. Atochin, Satoshi Kashiwagi
Summary: This study provides evidence that NIR-II laser enhances bioavailable NO in endothelial cells, suggesting its potential use in restoring endothelial NO and treating or preventing cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Soo Hee Lee, Seong-Ho Ok, Seung Hyun Ahn, Hyun-Jin Kim, Sung Il Bae, Ji-Yoon Kim, Kyeong-Eon Park, Yeran Hwang, Ju-Tae Sohn
Summary: This study found that lipid emulsion enhanced the vasoconstriction induced by dexmedetomidine by inhibiting nitric oxide synthesis, potentially through reducing dexmedetomidine concentration. Dexmedetomidine-induced nitric oxide synthesis may be caused by Src kinase-induced caveolin-1 phosphorylation leading to increased eNOS (Ser1177) phosphorylation and decreased eNOS (Thr495) phosphorylation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Chih-Hsien Wu, Yi-Lin Chiu, Chung-Yueh Hsieh, Guo-Shiang Tsung, Lian-Shan Wu, Cheng-Chung Cheng, Tsung-Neng Tsai
Summary: Cilostazol exerts antithrombotic and vasculoprotective effects in endothelial cells by activating KLF2 and SIRT1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shaimaa Maher, Lauren A. Smith, Celine A. El-Khoury, Haitham Kalil, Khalid Sossey-Alaoui, Mekki Bayachou
Summary: Nitric oxide (NO) is a small molecule that has a significant impact on various biological activities. It has been linked to cancer-related processes and has the potential to be used as an anti-cancer agent. In this study, NO-releasing materials were developed using modified sodium alginate hydrogel, which showed compatibility with cell growth. The release of NO from the hydrogel exhibited distinct kinetic features.
Article
Nutrition & Dietetics
Konstantinos Efthymakis, Giuseppina Bologna, Pasquale Simeone, Laura Pierdomenico, Giulia Catitti, Simone Vespa, Angelo Milano, Domenico De Bellis, Francesco Laterza, Assunta Pandolfi, Caterina Pipino, Michele Sallese, Marco Marchisio, Sebastiano Miscia, Matteo Neri, Paola Lanuti
Summary: Extracellular vesicles (EVs) play important roles in intercellular communication, homeostasis maintenance, and diseases. This study aimed to assess and characterize circulating EVs in celiac disease patients and patients on a gluten-free diet (GFD) using a novel flow cytometry technique. The results showed significantly increased total EV counts in both newly diagnosed celiac disease patients and patients on GFD, while endothelial-derived and epithelial-derived EV counts were significantly decreased in subjects on gluten exclusion. These findings indicate that EVs could serve as potential disease-specific biomarkers in celiac disease.
Article
Engineering, Biomedical
Luca Comuzzi, Margherita Tumedei, Tea Romasco, Morena Petrini, Kelvin I. Afrashtehfar, Francesco Inchingolo, Adriano Piattelli, Natalia Di Pietro
Summary: Short implants were introduced to reduce morbidity, treatment duration, and complex bone regeneration interventions in atrophic jaws and to improve patient-reported outcomes. This study aimed to determine the insertion torque (IT), removal torque (RT), and resonance frequency analysis (RFA) values of ultrashort (3 mm length), short (7 mm length), and standard implants (10 mm length) inserted in 1-, 2-, 3-, and 4-mm thickness polyurethane sheets with densities of 10, 20, and 30 pounds per cubic foot (PCF). The results showed that short-length implants had comparable or higher IT values compared to the standard-length implants in most experimental conditions. Ultrashort-length implants demonstrated similar primary stability to standard implants in lower thicknesses. These findings suggest the potential application of short and ultrashort implants in critical atrophy of the posterior jaws.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Emira D'Amico, Tania Vanessa Pierfelice, Rosa Amoroso, Ivana Cacciatore, Camillo D'Arcangelo, Stefania Lepore, Simonetta D'Ercole, Natalia Di Pietro, Annalisa Di Rienzo, Morena Petrini, Adriano Piattelli, Alessia Ricci, Susi Zara, Antonio Di Stefano, Giovanna Iezzi, Barbara De Filippis
Summary: Recently, research has focused on finding new approaches to manage oral wound healing. The use of resveratrol (RSV) as a drug is limited due to its poor bioavailability. This study aimed to investigate a series of RSV derivatives (1a-j) with better pharmacokinetic profiles. Among them, derivatives 1d and 1h showed increased cell viability compared to RSV. Further studies demonstrated that both 1d and 1h exhibited positive effects on cell viability, proliferation, and gene expression in various cells involved in oral wound healing. Additionally, 1d and 1h showed promising physicochemical properties and stability, indicating their potential as RSV-based agents for oral tissue repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Valeria Schiavone, Tea Romasco, Nadia Di Pietrantonio, Stefania Garzoli, Carola Palmerini, Pamela Di Tomo, Caterina Pipino, Domitilla Mandatori, Rossella Fioravanti, Elena Butturini, Manuela Sabatino, Maria Pompea Antonia Baldassarre, Rino Ragno, Assunta Pandolfi, Natalia Di Pietro
Summary: Essential oils derived from aromatic plants have been found to improve the lipid and glycemic profile of patients with diabetes mellitus. This study investigated the potential anti-inflammatory effect of anise and laurel essential oils on endothelial cells isolated from females with gestational diabetes mellitus. The results showed that both essential oils significantly reduced monocyte adhesion, VCAM-1 expression, and NF-κB p65 nuclear translocation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Luca Comuzzi, Margherita Tumedei, Ugo Covani, Tea Romasco, Morena Petrini, Lorenzo Montesani, Adriano Piattelli, Natalia Di Pietro
Summary: Bone characteristics, implant macrogeometry, and drilling technique are important factors for implant primary stability. This in vitro study evaluated the effects of under-preparation on insertion torque, removal torque, and resonance frequency analysis of conical implants. Results showed that proper site preparation can improve implant primary stability.
APPLIED SCIENCES-BASEL
(2023)
Review
Oncology
Sara Pagotto, Pasquale Simeone, Davide Brocco, Giulia Catitti, Domenico De Bellis, Simone Vespa, Natalia Di Pietro, Lisa Marinelli, Antonio Di Stefano, Serena Veschi, Laura De Lellis, Fabio Verginelli, Francesco Kaitsas, Manuela Iezzi, Assunta Pandolfi, Rosa Visone, Nicola Tinari, Ignazio Caruana, Mauro Di Ianni, Alessandro Cama, Paola Lanuti, Rosalba Florio
Summary: This review highlights the potential of CAR-T-derived extracellular vesicles (EVs) as therapeutic agents in tumors. CAR-T lymphocytes have shown potential effectiveness in immunotherapy against refractory neoplastic diseases. However, physical barriers, hostile microenvironment, and tumor heterogeneity limit their efficacy against solid tumors. The use of CAR-T-derived EVs as therapeutic agents may enhance CAR-T homing and overcome some adverse effects.
Review
Pharmacology & Pharmacy
Letizia Pelusi, Domitilla Mandatori, Leonardo Mastropasqua, Luca Agnifili, Marcello Allegretti, Mario Nubile, Assunta Pandolfi
Summary: Ocular drug delivery remains a challenge due to the limitations of conventional dosage forms and the anatomical complexity of the eye. Recent developments in materials and technologies have led to the creation of various ocular drug delivery systems, such as inserts, contacts, and intraocular lenses, which show promise for future clinical applications.
Article
Biochemistry & Molecular Biology
Tea Romasco, Pier Michele Mandrillo, Erica Morsut, Margherita Tumedei, Domitilla Mandatori, Morena Petrini, Maria Cristina Curia, Francesco De Angelis, Camillo D'Arcangelo, Adriano Piattelli, Natalia Di Pietro
Summary: Maintaining the health of periodontal and peri-implant soft tissues is crucial. Collagen-based medical products have shown to be safe and effective in preventing connective tissue alterations and related diseases. This study evaluated the effects of Dental SKIN BioRegulation, an injectable medical device composed of collagen, on human gingival fibroblasts.
Article
Chemistry, Physical
Luca Comuzzi, Margherita Tumedei, Natalia Di Pietro, Tea Romasco, Hamid Heydari Sheikh Hossein, Lorenzo Montesani, Francesco Inchingolo, Adriano Piattelli, Ugo Covani
Summary: This study aimed to compare the stability of three different types of implants in different densities of polyurethane blocks. The results showed that TAC conical implants demonstrated better primary stability in low-density models.
Article
Chemistry, Multidisciplinary
Natalia Di Pietro, Mario Ceddia, Tea Romasco, Nilton De Bortoli Jr, Bruno Freitas Mello, Margherita Tumedei, Alessandro Specchiulli, Adriano Piattelli, Bartolomeo Trentadue
Summary: The reason for peri-implant bone resorption is still unknown, but it may be related to bacterial contamination at the micro-gap level between the implant and abutment. Placement of subcrestal implants can provide aesthetic advantages, but there are conflicting results on bone resorption in the literature. Finite Element Analysis showed that subcrestally placed implants had lower stresses in the cortical bone compared to equicrestally placed implants.
APPLIED SCIENCES-BASEL
(2023)
Article
Cell Biology
Marika Berardini, Luisa Gesualdi, Caterina Morabito, Francesca Ferranti, Anna Reale, Michele Zampieri, Katsiaryna Karpach, Antonella Tinari, Lucia Bertuccini, Simone Guarnieri, Angela Catizone, Maria A. Mariggio, Giulia Ricci
Summary: The study shows that simulated microgravity exposure can lead to accumulation of reactive oxygen species in TCam-2 cells, along with increased protein and lipid oxidation, and altered mitochondria structure. However, the cells are capable of triggering compensatory mechanisms to overcome the effects of altered gravitational force.
Article
Engineering, Biomedical
Ugo Covani, Enrica Giammarinaro, Natalia Di Pietro, Simona Boncompagni, Giorgia Rastelli, Tea Romasco, Eugenio Velasco-Ortega, Alvaro Jimenez-Guerra, Giovanna Iezzi, Adriano Piattelli, Simone Marconcini
Summary: This study compared two implants with different healing abutment geometries and quantified the three-dimensional topography and morphological properties of collagen using transmission electron microscopy. The results showed that a concave abutment design promotes the formation of a well-organized peri-implant collagen structure.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Engineering, Multidisciplinary
Alessandro Cipollina, Mario Ceddia, Natalia Di Pietro, Francesco Inchingolo, Margherita Tumedei, Tea Romasco, Adriano Piattelli, Alessandro Specchiulli, Bartolomeo Trentadue
Summary: Extreme atrophy of the maxilla remains a challenge for clinicians. The premaxillary device (PD) technique offers a simpler approach to reconstruct severely atrophic maxillae, aiming to avoid more complicated and risky surgical procedures. Finite element analysis (FEA) demonstrated that using a PD allows for an optimal distribution of stresses on the basal bone, reducing the risk of bone resorption or implant failure.
Article
Biochemistry & Molecular Biology
Luca Comuzzi, Mario Ceddia, Natalia Di Pietro, Francesco Inchingolo, Angelo Michele Inchingolo, Tea Romasco, Margherita Tumedei, Alessandro Specchiulli, Adriano Piattelli, Bartolomeo Trentadue
Summary: This study used three-dimensional finite element analysis to investigate the biomechanical effects of different positions of dental implants relative to the alveolar crest. The findings suggest that placing the implant subcrestally (-1 to -2 mm) can help reduce peri-implant bone resorption and improve long-term implant success.