Article
Biochemistry & Molecular Biology
Jeroen Dobbelaere, Tiffany Y. Su, Balazs Erdi, Alexander Schleiffer, Alexander Dammermann
Summary: Cilia are cellular projections that perform sensory and motile functions in eukaryotic cells. In this study, a set of 386 human genes associated with cilium assembly or motility were identified by analyzing the presence and absence pattern in the genomes of diverse eukaryotes. The novel genes were further characterized and found to be related to cilium defects in fruit flies and nematodes. This dataset defines the core set of genes required for cilium assembly and motility across eukaryotes and provides a valuable resource for future studies.
Article
Multidisciplinary Sciences
Julia Zwirchmayr, Cristina D. Cruz, Ulrike Grienke, Paeivi Tammela, Judith M. Rollinger
Summary: The root extract of Peucedanum ostruthium (PO-E) was found to be a promising antibacterial source against Staphylococcus aureus. It was also shown to significantly decrease the survival of Caenorhabditis elegans. Through the biochemometric approach ELINA, the anti-infective constituent(s) targeting S. aureus and C. elegans in the multicomponent mixture PO-E were identified.
Article
Genetics & Heredity
Armando Moreno, Allison Taffet, Elissa Tjahjono, Quinton L. L. Anderson, Natalia V. V. Kirienko
Summary: Mitochondria play crucial roles in cellular health and metabolism, as well as programmed cell death. This study used RNAi to disrupt a series of genes related to mitochondrial health in the model organism Caenorhabditis elegans, and found that disruption of these genes resulted in mitochondrial dysfunction and exacerbated α-synuclein aggregation in a Parkinson's disease model. These findings provide a foundation for identifying new mechanisms that support mitochondrial and cellular homeostasis.
Article
Agronomy
Rongrong Guo, Ling Lin, Guiyuan Huang, Xiaofang Shi, Rongfu Wei, Jiayu Han, Sihong Zhou, Ying Zhang, Taili Xie, Xianjin Bai, Xiongjun Cao
Summary: This study used RNA-seq to investigate the transcriptional responses of heat-stressed grapevine leaves, revealing the effects of heat stress on photosynthesis and abscisic acid biosynthesis, metabolism, and signal transduction pathways.
Article
Biochemistry & Molecular Biology
Marlon Kazmierczak, Carlota Farre i Diaz, Andreas Ofenbauer, Sergej Herzog, Baris Tursun
Summary: Investigating the manifold gene functions in biological systems requires simultaneous depletion of two or more gene activities. The CONJUDOR technique allows for robust double RNAi with high-throughput, improving efficiency and versatility in RNAi screens.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Alexandra R. Willis, Winnie Zhao, Ronesh Sukhdeo, Lina Wadi, Hala Tamim El Jarkass, Julie M. Claycomb, Aaron W. Reinke
Summary: Parental infection can result in offspring with enhanced immunity, as shown in Caenorhabditis elegans infected with N. parisii. The offspring's immunity is induced by the parental transcriptional response to infection, protecting them against multiple classes of pathogens.
Article
Multidisciplinary Sciences
Mark G. Sterken, Marijke H. van Wijk, Elizabeth C. Quamme, Joost A. G. Riksen, Lucinda Carnell, Laura D. Mathies, Andrew G. Davies, Jan E. Kammenga, Jill C. Bettinger
Summary: The study examined the transcriptional responses of Caenorhabditis elegans to ethanol exposure across different timepoints, revealing that short exposures induced expression of metabolic enzymes while longer exposures had more profound effects on the transcriptome. Genes involved in cilia function were regulated by longer ethanol exposure, which may play a role in the deleterious effects of chronic ethanol consumption in humans. Additionally, food deprivation-induced gene expression changes were somewhat alleviated by ethanol, supporting previous observations that worms can use ethanol as a calorie source.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Elisabeth Rackles, Michael Witting, Ignasi Forne, Xing Zhang, Judith Zacherl, Simon Schrott, Christian Fischer, Jonathan J. Ewbank, Christof Osman, Axel Imhof, Stephane G. Rolland
Summary: Research shows the existence of organelle-specific retrograde signaling for peroxisomes, which plays an important role in responding to stress and infection, regulating lipid metabolism, immunity, and potentially acting as a protective mechanism.
Article
Multidisciplinary Sciences
Brandon M. Murareanu, Noelle Antao, Winnie Zhao, Aurore Dubuffet, Hicham El Alaoui, Jessica Knox, Damian C. Ekiert, Gira Bhabha, Peter J. Roy, Aaron W. Reinke
Summary: In this study, a high-throughput screening method was developed to identify chemical inhibitors of microsporidia infection. Through experiments on Caenorhabditis elegans, 11 potential microsporidia inhibitors were identified, among which five compounds prevent microsporidia infection by inhibiting spore firing.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Bingying Wang, Taruna Pandey, Yong Long, Sofia E. Delgado-Rodriguez, Matthew D. Daugherty, Dengke K. Ma
Summary: This study reveals the detoxification mechanism of cyanide in the nematode C. elegans, which is regulated by a series of genes in a genetic pathway. The study also suggests that the two genes, cysl-1 and cysl-2, involved in this detoxification mechanism, may have been acquired through horizontal gene transfer from green algae and functionally co-opted in protecting the host against amygdalin toxicity.
Article
Environmental Sciences
Lingyan Sun, Yanping Zhou, Chunyan Wang, Yaguang Nie, An Xu, Lijun Wu
Summary: This study investigated the toxic effects of RDX on Caenorhabditis elegans and its future generations, as well as the signaling pathways involved. Exposure to RDX led to increased reactive oxygen species production, germ cell apoptosis, and decreased egg laying. Mutations in specific genes and RNA sequencing revealed the apoptosis signaling pathway and the metabolism of RDX in the nematodes. The transgenerational effects on reproductive toxicity were associated with specific gene expressions. These findings provide a basis for further understanding the ecological risks of energetic compounds in the environment.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Jiechen Yin, Zihai Jian, Guangcan Zhu, Xiaojin Yu, Yuepu Pu, Lihong Yin, Dayong Wang, Yuanqing Bu, Ran Liu
Summary: PFOS and PFOA exposure was found to reduce brood size, germ cell number, spermatid size, and motility, as well as increase malformation rate of spermatids. PFOS showed higher levels of damage than PFOA in germ cells at lower exposure levels, potentially disrupting various stages of spermatogenesis. Loss-of-function studies revealed that spe-10 gene may play a specific role in PFOS- or PFOA-induced reproductive toxicity, providing valuable data for risk assessment of these pollutants.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Environmental Sciences
Shuting Wang, Huanliang Liu, Man Qu, Dayong Wang
Summary: Neurotransmission related signals, specifically tyramine and glutamate, play crucial roles in regulating the toxicity of nanoplastics in Caenorhabditis elegans. Tyramine induces toxicity while glutamate alleviates toxicity, with both signals being mediated through neurons. Tyramine receptor TYRA-2 and glutamate receptors GLR-4 and GLR-8 act upstream of specific signaling pathways to regulate nanoplastic toxicity.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Multidisciplinary Sciences
Helena Rawsthorne-Manning, Fernando Calahorro, Patricia G. Izquierdo, Philippe Tardy, Thomas Boulin, Lindy Holden-Dye, Vincent O'Connor, James Dillon
Summary: This article introduces a method for increasing the efficiency of CRISPR/Cas9 editing using the model organism C. elegans. The study found that using co-CRISPR 'marker' genes can facilitate the selection of worms with CRISPR events in the target gene. However, sequencing analysis revealed that co-CRISPR genes may lead to genomic rearrangements, which should be considered before downstream analysis of gene function.
Article
Genetics & Heredity
Luke M. Chandler, Keith P. Choe
Summary: The study investigates the impact of mutation or loss of 6 extracellular matrix collagen genes on annular furrows in adult C. elegans cuticles. It also explores the activation of stress response genes and the morphological changes in body shape and furrow organization. The findings suggest a progressive cuticle phenotype and the role of furrow disruption as a signal in L4 larvae and adults.
G3-GENES GENOMES GENETICS
(2022)
Article
Parasitology
Russell W. Avramenko, Elizabeth M. Redman, Lynsey Melville, Yvonne Bartley, Janneke Wit, Camila Queiroz, Dave J. Bartley, John S. Gilleard
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2019)
Article
Parasitology
Neil D. Sargison, Elizabeth Redman, Alison A. Morrison, David J. Bartley, Frank Jackson, Eric Hoberg, John S. Gilleard
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2019)
Article
Parasitology
Elizabeth Redman, Camila Queiroz, David J. Bartley, Michel Levy, Russell W. Avramenko, John Stuart Gilleard
VETERINARY PARASITOLOGY
(2019)
Article
Parasitology
Carly D. Barone, Janneke Wit, Eric P. Hoberg, John S. Gilleard, Dante S. Zarlenga
VETERINARY PARASITOLOGY
(2020)
Article
Parasitology
Julie Sanchez, Guha Dharmarajan, Melissa M. George, Cassan Pulaski, Adrian J. Wolstenholme, John S. Gilleard, Ray M. Kaplan
VETERINARY PARASITOLOGY
(2020)
Article
Parasitology
Emma A. Borkowski, Jacob Avula, Niel A. Karrow, Paula Menzies, William Sears, Elizabeth M. Redman, Brandon N. Lillie, John S. Gilleard, Andrew S. Peregrine
VETERINARY PARASITOLOGY
(2020)
Article
Parasitology
Emma A. Borkowski, Elizabeth M. Redman, Rebecca Chant, Jacob Avula, Paula Menzies, Niel A. Karrow, Brandon N. Lillie, William Sears, John S. Gilleard, Andrew S. Peregrine
VETERINARY PARASITOLOGY
(2020)
Article
Parasitology
Umer Chaudhry, E. M. Redman, Ray Kaplan, Thomas Yazwinski, Neil Sargison, John S. Gilleard
VETERINARY PARASITOLOGY
(2020)
Article
Parasitology
Melissa M. George, Adriano F. Vatta, Sue B. Howell, Bob E. Storey, Ciaran J. McCoy, Adrian J. Wolstenholme, Elizabeth M. Redman, John S. Gilleard, Ray M. Kaplan
Summary: The study successfully replaced a population of Haemonchus contortus resistant to multiple anthelmintics with a susceptible isolate, but resistance re-emerged within a short period of time. Genetic changes may have contributed to this reversion.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2021)
Article
Infectious Diseases
Maria A. Santa, Andrew M. Rezansoff, Rebecca Chen, John S. Gilleard, Marco Musiani, Kathreen E. Ruckstuhl, Alessandro Massolo
Summary: The study found Echinococcus multilocularis is expanding its distribution in North America and the European-type haplotype is prevalent in western Canada, increasing the risk of human infection. Deep amplicon sequencing is a valuable tool for detecting co-infections and assessing the distribution of different haplotypes. The study highlights the importance of next-generation sequencing technologies for understanding the geographic expansion patterns of the parasite.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Parasitology
Pablo D. Jimenez Castro, Abhinaya Venkatesan, Elizabeth Redman, Rebecca Chen, Abigail Malatesta, Hannah Huff, Daniel A. Zuluaga Salazar, Russell Avramenko, John S. Gilleard, Ray M. Kaplan
Summary: Ancylostoma caninum infections in greyhound dogs in the USA exhibit high levels of multiple-drug resistance, with a high prevalence and infection intensity observed within the greyhound population.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2021)
Article
Microbiology
Roz Laing, Stephen R. Doyle, Jennifer McIntyre, Kirsty Maitland, Alison Morrison, David J. Bartley, Ray Kaplan, Umer Chaudhry, Neil Sargison, Andy Tait, James A. Cotton, Collette Britton, Eileen Devaney
Summary: Through crossbreeding and gene expression analysis, we have identified certain genes associated with ivermectin resistance and proposed a novel mechanism that allows resistant worms to survive treatment. This is crucial for understanding ivermectin resistance and response to treatment in parasitic helminths.
Article
Veterinary Sciences
Jennifer McIntyre, Laura Miskell, Eric R. Morgan, Fiona Lovatt, Roz Laing
Summary: This study investigated the use and effectiveness of moxidectin against gastrointestinal nematodes among Scottish sheep farmers. The results showed that although the majority of farmers used moxidectin, there was variation in their knowledge levels, and most farmers did not consistently follow the guidelines for sustainable control of parasites.
Article
Parasitology
Alistair Antonopoulos, Stephen R. Doyle, David J. Bartley, Alison A. Morrison, Ray Kaplan, Sue Howell, Cedric Neveu, Valentina Busin, Eileen Devaney, Roz Laing
Summary: Haemonchus contortus is a parasitic nematode that infects small ruminants and causes health issues and economic losses in the livestock industry. The treatment for this parasite primarily relies on broad-spectrum anthelmintics, but resistance has emerged. Levamisole (LEV) is an important treatment option, as resistance to LEV is less common. Genetic variation at the acr-8 locus has been implicated in LEV resistance.
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2022)
Article
Biology
Stephen R. Doyle, Alan Tracey, Roz Laing, Nancy Holroyd, David Bartley, Wojtek Bazant, Helen Beasley, Robin Beech, Collette Britton, Karen Brooks, Umer Chaudhry, Kirsty Maitland, Axel Martinelli, Jennifer D. Noonan, Michael Paulini, Michael A. Quail, Elizabeth Redman, Faye H. Rodgers, Guillaume Salle, Muhammad Zubair Shabbir, Geetha Sankaranarayanan, Janneke Wit, Kevin L. Howe, Neil Sargison, Eileen Devaney, Matthew Berriman, John S. Gilleard, James A. Cotton
COMMUNICATIONS BIOLOGY
(2020)