Article
Multidisciplinary Sciences
Bindu J. Subhadra, Dianjun Cao, Roderick Jensen, Clayton Caswell, Thomas Inzana
Summary: This study identified and partially characterized Hfq-associated sRNAs in Histophilus somni. Co-immunoprecipitation and sRNA sequencing identified 100 putative sRNAs, with 16 present in the pathogenic strain. Bioinformatics analysis suggested that certain sRNAs could bind to genes involved in virulence/biofilm formation. Further experiments confirmed sRNA-Hfq binding and determined the transcriptional start sites. This investigation provides insight into the regulatory roles of sRNAs in H. somni virulence and biofilm formation.
Article
Microbiology
Gregory P. Harhay, Dayna M. Harhay, Kerry D. Brader, Timothy P. L. Smith
Summary: Histophilus somni is a Gram-negative bacterial organism associated with various diseases in ruminants. A unique intervening sequence (IVS) was found in its 23S rRNA gene, which when excised, may impact the biological functions of the organism. The fragmented rRNA remains functional, suggesting this discovery could provide new insights into regulating biological processes of the bacterium.
MICROBIOLOGY SPECTRUM
(2021)
Article
Veterinary Sciences
Ana Pascual-Garrigos, Murali Kannan Maruthamuthu, Aaron Ault, Josiah Levi Davidson, Grigorii Rudakov, Deepti Pillai, Jennifer Koziol, Jon P. Schoonmaker, Timothy Johnson, Mohit S. Verma
Summary: This study modified a LAMP assay for detecting BRD bacterial pathogens on a farm. The method offers high sensitivity and specificity, providing rapid on-site diagnostics.
VETERINARY RESEARCH
(2021)
Article
Microbiology
Tamara Alhamami, Wai Yee Low, Yan Ren, Kara Taylor, Manouchehr Khazandi, Tania Veltman, Henrietta Venter, Mandi Carr, Conny Turni, Sam Abraham, Darren J. Trott
Summary: This study investigated the antimicrobial susceptibility and phylogenetic relationships of H. somni isolates from Australian feedlot cattle. The results showed that these isolates did not exhibit resistance to the tested antimicrobials and had limited genetic diversity.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Jose J. Rivera Rivas, Charles J. Czuprynski
Summary: Histophilus somni is a Gram-negative coccobacillus that causes diffuse vasculitis and intravascular thrombosis in cattle. Our study demonstrates that exposure to H. somni cells causes monocyte-derived macrophages to release microparticles with pro-coagulant activity, potentially amplifying thrombus formation.
VETERINARY MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Yuichi Ueno, Kenta Suzuki, Yuji Takamura, Kaori Hoshinoo, Daisuke Takamatsu, Ken Katsuda
Summary: Histophilus somni, a bacteria causing various diseases in cattle, showed relatively low rates of antimicrobial resistance overall, but isolates from cattle with respiratory diseases exhibited higher resistance to multiple antibiotics, possibly due to the presence of specific resistance genes. The study also suggested that integrative and conjugative elements (ICEs) may contribute to the spread of resistance genes in certain genetic groups of H. somni strains.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Immunology
Yu Pan, Shivakumara Siddaramappa, Indra Sandal, Allan Dickerman, Aloka B. Bandara, Thomas J. Inzana, Guy H. Palmer
Summary: The LuxS gene plays a role in biofilm formation and virulence in H. somni, affecting sensitivity in serum and attenuation in a mouse model. While the LuxS mutant showed differences in LOS synthesis compared to the wild-type, no major changes were observed in terms of outer membrane protein profiles, biofilm formation, EPS production, or intracellular survival.
INFECTION AND IMMUNITY
(2021)
Article
Immunology
Selwyn Arlington Headley, Juliana Torres Tomazi Fritzen, Vinicius Rodrigues Bon, Ana Aparecida Correa Xavier, Alais Maria Dall Agnol, Natalia Zaparoli Zucoloto, Flavia Helena Pereira Silva, Julia Raisa Ximenes Figueiredo, Alice Fernandes Alfieri, Werner Okano, Amauri Alcindo Alfieri
Summary: Bovine gammaherpesvirus 6 (BoGHV6) is a virus associated with proliferative diseases in cattle. This study found that the majority of evaluated fetuses were infected with BoGHV6, possibly through vertical transmission. Fetuses co-infected with Histophilus somni were also found to have other pathogens. Phylogenetic analysis showed that the identified strain of BoGHV6 is similar to wild type strains circulating in Brazil, but different from other related viruses.
MICROBIAL PATHOGENESIS
(2022)
Article
Genetics & Heredity
Xupeng Li, Yanbin Bai, Jingsheng Li, Zongchang Chen, Yong Ma, Bingang Shi, Xiangmin Han, Yuzhu Luo, Jiang Hu, Jiqing Wang, Xiu Liu, Shaobin Li, Zhidong Zhao
Summary: This study investigates the miRNA regulation of unsaturated fatty acid synthesis in bovine adipocytes by transfecting si-ACSL1 and NC-ACSL1 in bovine preadipocytes. The results indicate that the differentially expressed miRNAs target genes involved in biological regulation and metabolic processes. Moreover, these miRNAs are enriched in metabolic pathways, fatty acid metabolism, and other related pathways.
FRONTIERS IN GENETICS
(2022)
Article
Food Science & Technology
Yingshu He, Ruixi Chen, Yan Qi, Joelle K. Salazar, Shimei Zhang, Mary Lou Tortorello, Xiangyu Deng, Wei Zhang
Summary: Salmonella enterica, especially serotypes Newport and Typhimurium, are frequently associated with foodborne disease outbreaks linked to fresh-cut fruits. Despite strong survivability on various fresh-cut fruits under refrigeration, S. enterica displays variable survival behaviors, potentially regulated by differential gene expression related to carbon utilization and metabolic pathways. This study highlights the importance of understanding S. enterica survival mechanisms on fresh-cut produce and their potential implications for food safety and human health.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mai Farghaly, Michael F. Hynes, Mohammad Nazari, Sylvia Checkley, Karen Liljebjelke
Summary: Integrative and conjugative elements (ICEs) are important in facilitating horizontal gene transfer and the dissemination of antimicrobial resistance between bacteria. A study identified an ICEHs02 in Histophilus somni that carries multiple antimicrobial resistance genes and can be transferred to other bacteria. The study found that tetracycline and ciprofloxacin induction significantly increased the transfer rates of ICEHs02, and the acquisition of ICEHs02 reduced the relative fitness of the host bacteria.
CANADIAN JOURNAL OF MICROBIOLOGY
(2022)
Review
Microbiology
Axel B. Janssen, Vincent de Bakker, Rieza Aprianto, Vincent Trebosc, Christian Kemmer, Michel Pieren, Jan-Willem Veening
Summary: This study investigated the importance of the EnvZ/OmpR regulatory system in Klebsiella pneumoniae for fitness, gene regulation, virulence, and infection. The results showed that OmpR plays a crucial role in the virulence and lung infection of K. pneumoniae, and its regulon includes important virulence factors. Furthermore, deletion of ompR gene leads to a stronger antibacterial transcriptional response in human lung epithelial cells.
MICROBIOLOGY SPECTRUM
(2023)
Article
Medicine, Research & Experimental
Maria C. Garcia-Hidalgo, Rafael Pelaez, Jessica Gonzalez, Sally Santisteve, Ivan D. Bebnitez, Marta Molinero, Manel Perez-Pons, Thalia Belmonte, Gerard Torres, Anna Moncusi-Moix, Clara Gort-Paniello, Maria Aguila, Faty Seck, Paola Carmona, Jesus Caballero, Carme Barbera, Adrian Ceccato, Laia Fernandez-Barat, Ricard Ferrer, Dario Garcia-Gasulla, Jose Angel Lorente-Balanza, Rosario Menendez, Ana Motos, Oscar Penuelas, Jordi Riera, Jesus F. Bermejo-Martin, Antoni Torres, Ferran Barbe, David de Gonzalo-Calvo, Ignacio M. Larrayoz
Summary: This study identified the molecular basis associated with severe lung diffusion involvement in survivors of SARS-CoV-2-induced ARDS using transcriptomic analysis. The findings provide insights into the biological pathways and immune cells related to post-acute pulmonary sequelae, and suggest potential targets for intervention and biomarker development.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Agriculture, Dairy & Animal Science
Riccardo Compiani, Silvia Grossi, Carlo Angelo Sgoifo Rossi
Summary: Histophilus somni is a major bacterial pathogen causing reproductive, cardiovascular, and respiratory diseases in cattle. Understanding its virulence factors and vaccination strategies is crucial for controlling H. somni outbreaks.
LARGE ANIMAL REVIEW
(2023)
Article
Genetics & Heredity
Adam M. H. Young, Natsuhiko Kumasaka, Fiona Calvert, Timothy R. Hammond, Andrew Knights, Nikolaos Panousis, Jun Sung Park, Jeremy Schwartzentruber, Jimmy Liu, Kousik Kundu, Michael Segel, Natalia A. Murphy, Christopher E. McMurran, Harry Bulstrode, Jason Correia, Karol P. Budohoski, Alexis Joannides, Mathew R. Guilfoyle, Rikin Trivedi, Ramez Kirollos, Robert Morris, Matthew R. Garnett, Ivan Timofeev, Ibrahim Jalloh, Katherine Holland, Richard Mannion, Richard Mair, Colin Watts, Stephen J. Price, Peter J. Kirkpatrick, Thomas Santarius, Edward Mountjoy, Maya Ghoussaini, Nicole Soranzo, Omer A. Bayraktar, Beth Stevens, Peter J. Hutchinson, Robin J. M. Franklin, Daniel J. Gaffney
Summary: By analyzing primary human microglia isolated from 141 patients undergoing neurosurgery, the study found how age, sex, clinical pathology, and genetic variants influence microglia gene expression. Using a macrophage model, a candidate causal variant for Alzheimer's disease at the BIN1 locus was fine-mapped. This research provides a population-scale transcriptional map of the important cell for human CNS development and disease.