Article
Cell Biology
Wenbo He, Ke Wu, Zhenlin Ouyang, Yixin Bai, Wen Luo, Di Wu, Hao An, Yucheng Guo, Min Jiao, Qian Qin, Jiaxin Zhang, Yi Wu, Junjun She, Peter M. Hwang, Fang Zheng, Li Zhu, Yurong Wen
Summary: The Type VI secretion system is commonly used by Gram-negative bacteria to inject toxic effectors into nearby cells. The core components of the system, Hcp, VgrG, or PAAR, allow for the loading of various effectors onto the delivery tube. Through cryo-EM and crystal structure analyses, this study provides insights into the assembly, loading, and firing of the T6SS nanomachine, contributing to bacterial interspecies competition and host interactions.
Article
Multidisciplinary Sciences
Li Li, Zuxian Xu, Ruipin Cao, Jiaxin Li, Chang-Jer Wu, Yinglu Wang, Hu Zhu
Summary: We investigated the influence of hydroxyl groups on the anti-quorum-sensing (anti-QS) and anti-biofilm activity of cyclic dipeptides against Pseudomonas aeruginosa PAO1. The presence of hydroxyl groups improved the inhibition of virulence factors and cytotoxicity, but reduced the ability to inhibit biofilm formation. These cyclic dipeptides interacted with the QS-related protein LasR, and the introduction of hydroxyl groups enhanced their self-assembly ability.
Article
Biochemistry & Molecular Biology
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Agnieszka Mikucka
Summary: This study assessed the susceptibility and virulence factor gene frequency of clinical P. aeruginosa strains causing bloodstream infections. The results showed that these strains were resistant to multiple antibiotics and had diverse compositions of virulence factor genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecinska-Pirog, Eugenia Gospodarek-Komkowska
Summary: The study investigated the frequency and genotype distribution of 10 virulence factor genes in 107 non-duplicated carbapenem-resistant P. aeruginosa isolates. Variations were observed in the prevalence and distribution of virulence genes, especially in terms of their carbapenemase synthesis ability and the strain origin.
Review
Microbiology
Alexandra Grote, Ashlee M. Earl
Summary: Many bacterial pathogens can form persistent infections, and high-throughput sequencing methods have enabled the study of their genetic evolution, revealing common trends in bacterial adaptation to persistent infections.
CURRENT OPINION IN MICROBIOLOGY
(2022)
Review
Immunology
Pamella Constantino-Teles, Albane Jouault, Lhousseine Touqui, Alessandra Mattos Saliba
Summary: Pseudomonas aeruginosa, an opportunistic pathogen, causes respiratory infections by utilizing various virulence factors, such as bacterial lipids and enzymes, and by interacting with host lipids. These mechanisms play important roles in the establishment and persistence of P. aeruginosa in the lungs, as well as in modulating host immune responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Ecology
Pawel Markwitz, Cedric Lood, Tomasz Olszak, Vera van Noort, Rob Lavigne, Zuzanna Drulis-Kawa
Summary: The study found that phage-resistance emergence in bacterial populations had a key ecological impact and could be influenced by self and cross-resistance mechanisms. Using seven phages with distinct receptors to infect five Pseudomonas aeruginosa strains, significant differences were observed in resistance mechanisms between strains, which were correlated with bacterial growth rates.
Article
Multidisciplinary Sciences
Jing Yang, Qing-Ming Qin, Paul de Figueiredo
Summary: This article introduces a newly developed automated fluorescence microscopy-based imaging method, combined with high-throughput image processing and statistical analysis, which can rapidly and accurately quantify bacterial adherence to host cells. Experimental results demonstrate that this method significantly reduces experimental workloads and timelines.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Microbiology
Georgia Doing, Alexandra J. J. Lee, Samuel L. Neff, Taylor Reiter, Jacob D. Holt, Bruce A. Stanton, Casey S. Greene, Deborah A. Hogan
Summary: In this study, thousands of Pseudomonas aeruginosa RNA sequencing gene expression profiles were reanalyzed to create a powerful tool for hypothesis generation and testing. Raw sequence data were uniformly processed and validated using the Salmon pseudoaligner. Filtering criteria were developed to exclude samples with aberrant gene expression and normalization steps greatly improved gene expression correlations. The resulting compendia and algorithm for incorporating new data provide useful tools for P. aeruginosa RNA-seq analysis.
Article
Engineering, Biomedical
Ziv Azoulay, Polina Aibinder, Ayala Gancz, Jacob Moran-Gilad, Shiri Navon-Venezia, Hanna Rapaport
Summary: FKF, a tripeptide with potential antimicrobial activity, forms a pure peptide hydrogel phase made of natural amino acids. The hydrogel exhibits bactericidal activity against E. coli and P. aeruginosa, and reduces P. aeruginosa contamination in a rat skin lesion model.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Javier Macho Rendon, Rocio Rebollido-Rios, Marc Torrent Burgas
Summary: Protein-protein interactions play important roles in cellular processes. To address the limited knowledge of host-pathogen interactomes, we developed HPIPred, a numerical encoding-based host-pathogen PPI prediction tool. Through screening the entire proteomes of Homo sapiens and Pseudomonas aeruginosa PAO1, we generated a highly connected network topology of host-pathogen interactome using HPIPred.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Biology
Hala Tamim El Jarkass, Calvin Mok, Michael R. Schertzberg, Andrew G. Fraser, Emily R. Troemel, Aaron W. Reinke
Summary: This study identified a secreted protein used by microsporidia to promote host invasion and found that this intestinal protein plays a crucial role in the infection of microsporidia. The results also suggest that there may be evolutionary trade-offs in host defense against multiple classes of pathogens.
Article
Microbiology
Jiangfeng Zhao, Nils Hellwig, Bardya Djahanschiri, Radhika Khera, Nina Morgner, Ingo Ebersberger, Jingkang Wang, Hartmut Michel
Summary: This study presents the results of phylogenetic and functional studies on the PACE family transporter PA2880 from Pseudomonas aeruginosa. It demonstrates that PA2880 mainly exists as a dimer in solution and its dimeric state is essential for its proper function. Furthermore, electrogenicity studies reveal that the chlorhexidine/H+ antiport process is electrogenic and the function of several highly conserved residues was investigated.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Calvin K. Lee, William C. Schmidt, Shanice S. Webster, Jonathan W. Chen, George A. O'Toole, Gerard C. L. Wong
Summary: This study reveals that changes in c-di-GMP levels in bacterial biofilms are not the sole factor determining surface commitment, as oscillations in c-di-GMP levels can propagate across generations. Additionally, the study demonstrates that modulation of c-di-GMP signals and cooperative surface commitment can be influenced by varying the interaction strength between PilO and SadC.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Yongzhen Zhang, Liming Wang, Herman S. Overkleeft, Gijsbert A. van der Marel, Jeroen D. C. Codee
Summary: This study developed a synthetic chemistry method to create well-defined Pel oligosaccharides. These oligosaccharides can be used to investigate the biosynthesis of Pel polysaccharides and may have applications as diagnostic tools or components for glycoconjugate vaccines.
FRONTIERS IN CHEMISTRY
(2022)
Article
Immunology
Alexandre Elabbadi, Stephane Pont, Charlotte Verdet, Patrick Plesiat, Francois Cretin, Guillaume Voiriot, Muriel Fartoukh, Michel Djibre
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2020)
Article
Genetics & Heredity
Erwin Sentausa, Pauline Basso, Alice Berry, Annie Adrait, Gwendoline Bellement, Yohann Coute, Stephen Lory, Sylvie Elsen, Ina Attree
MICROBIAL GENOMICS
(2020)
Article
Biochemistry & Molecular Biology
Julian Trouillon, Erwin Sentausa, Michel Ragno, Mylene Robert-Genthon, Stephen Lory, Ina Attree, Sylvie Elsen
NUCLEIC ACIDS RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Quentin Bertrand, Viviana Job, Antoine P. Maillard, Lionel Imbert, Jean-Marie Teulon, Adrien Favier, Jean-Luc Pellequer, Philippe Huber, Ina Attree, Andrea Dessen
JOURNAL OF MOLECULAR BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Tuan-Dung Ngo, Caroline Perdu, Bakhos Jneid, Michel Ragno, Julia Novion Ducassou, Alexandra Kraut, Yohann Coute, Charles Stopford, Ina Attree, Arne Rietsch, Eric Faudry
JOURNAL OF MOLECULAR BIOLOGY
(2020)
Article
Microbiology
Stephane Pont, Nathan Fraikin, Yvan Caspar, Laurence Van Melderen, Ina Attree, Francois Cretin
Article
Microbiology
Julian Trouillon, Michel Ragno, Victor Simon, Ina Attree, Sylvie Elsen
Summary: Transcription factors (TFs) play a crucial role in bacterial response to new environmental conditions. A study on a family of eight TFs in Pseudomonas aeruginosa revealed XRE-cupin TFs act as inhibitors of neighboring genes, forming functional units for condition-specific metabolic pathways. Growth phenotypes of isogenic mutants highlighted new roles for PauR and PA0535 in polyamines and arginine metabolism. Phylogenetic analysis across bacterial species showed wide diversity in metabolic regulatory modules.
Article
Biochemistry & Molecular Biology
Oriane Moyne, Florence Castelli, Dominique J. Bicout, Julien Boccard, Boubou Camara, Benoit Cournoyer, Eric Faudry, Samuel Terrier, Dalil Hannani, Sarah Huot-Marchand, Claire Leger, Max Maurin, Tuan-Dung Ngo, Caroline Plazy, Robert A. Quinn, Ina Attree, Francois Fenaille, Bertrand Toussaint, Audrey Le Gouellec
Summary: The study used metabolomics analysis to explore the adaptive evolution of Pseudomonas aeruginosa in chronically infected cystic fibrosis patients, revealing significant associations between metabotypes, antibiotic resistance, virulence phenotypes, and clinical exacerbations. This identified promising biomarkers and therapeutic targets for difficult-to-treat P.a infections.
Article
Biochemistry & Molecular Biology
Samira Zouhir, Carlos Contreras-Martel, Daniel Maragno Trindade, Ina Attree, Andrea Dessen, Pauline Macheboeuf
Summary: The study found that in Pseudomonas aeruginosa, MagC may act as a link between MagD macroglobulin and peptidoglycan, providing stability and regulation for the entire complex.
Article
Multidisciplinary Sciences
Vincent Deruelle, Stephanie Bouillot, Viviana Job, Emmanuel Taillebourg, Marie-Odile Fauvarque, Ina Attree, Philippe Huber
Summary: The phospholipase ExoU from Pseudomonas aeruginosa acts on plasma membrane lipids in infected cells, causing membrane rupture and host cell necrosis. Once injected into the host cytoplasm, ExoU requires a host chaperone found on secretory vesicles to reach the plasma membrane and exert its phospholipase activity.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Alexandre Martins, Carlos Contreras-Martel, Manon Janet-Maitre, Mayara M. Miyachiro, Leandro F. Estrozi, Daniel Maragno Trindade, Caique C. Malospirito, Fernanda Rodrigues-Costa, Lionel Imbert, Viviana Job, Guy Schoehn, Ina Attree, Andrea Dessen
Summary: The elongasome, or Rod system, is a protein complex that controls cell wall formation in rod-shaped bacteria. MreC, a membrane-associated component of the elongasome, interacts with the cytoskeletal element MreB and regulates cell wall biosynthesis enzymes through self-association, contributing to the regulation of elongasome activity.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Julian Trouillon, Kook Han, Ina Attree, Stephen Lory
Summary: This study identified Hfq-interacting RNAs in different strains of Pseudomonas aeruginosa using RIP-seq technology. The results showed that most interactions are not conserved among different strains. Strain-specific Hfq targets were identified, including previously undescribed sRNAs.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Viviana Job, Laura Gomez-Valero, Adele Renier, Christophe Rusniok, Stephanie Bouillot, Viviane Chenal-Francisque, Erwan Gueguen, Annie Adrait, Mylene Robert-Genthon, Katy Jeannot, Peter Panchev, Sylvie Elsen, Marie-Odile Fauvarque, Yohann Coute, Carmen Buchrieser, Ina Attree
Summary: This study demonstrates the role of TPS toxins in bacteria-insect interactions in environmental Pseudomonas, and suggests that differences in CTDs may determine their specificity and mode of action towards eukaryotic cells.
Article
Microbiology
Manon Janet-Maitre, Stephane Pont, Frerich M. Masson, Serena Sleiman, Julian W. Trouillon, Mylene Robert-Genthon, Benoit Gallet, Chantal Dumestre-Perard, Sylvie Elsen, Christine Moriscot, Bart W. Bardoel, Suzan H. M. Rooijakkers, Francois Cretin, Ina Attree
Summary: This study found that multiple factors, including bacterial surface-attached molecules and adaptation to stress, as well as intracellular polyphosphates and biotin, significantly affect Pseudomonas aeruginosa's ability to withstand membrane attack complex (MAC)-dependent killing in human plasma. These findings highlight the importance of understanding the complex interaction between bacterial pathogens and the human immune system for developing effective antibacterial strategies.