Article
Biochemistry & Molecular Biology
Mengmeng Zheng, Jianmin Gao
Summary: Peptides, as a therapeutic modality between small molecules and antibodies, are gaining attention for their ability to combine the benefits of both. This study presents a novel phage library that incorporates two distinct designer groups, allowing the screening of peptides with diverse and non-natural functional motifs. The modification of phage-displayed peptides through sequential chemical reactions resulted in the discovery of potent peptide inhibitors against TEV protease.
ACS CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
David N. Philpott, Surath Gomis, Hansen Wang, Randy Atwal, Abdellali Kelil, Tanja Sack, Brandon Morningstar, Chris Burnie, Edward H. Sargent, Stephane Angers, Sachdev Sidhu, Shana O. Kelley
Summary: The rapid on-cell phage display platform presented in this study accelerates the development of high-performance human antibodies by mimicking the complex in vivo binding environment, improving screening efficiency and accuracy.
ACS CENTRAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Radoslaw W. Piast, Rafal M. Wieczorek, Nicola Marzec, Maciej Garstka, Aleksandra Misicka
Summary: Chondrocalcinosis is a metabolic disease caused by calcium pyrophosphate dihydrate crystals in the synovial fluid. The study aimed to investigate whether short peptides could dissolve these crystals, with one peptide named R25 showing hydrolytic activity towards the crystals, dependent on Mg2+ and working against soluble pyrophosphate species.
Article
Virology
Marwa Arbi, Imen Larbi, Jihen Nsiri, Imen El Behi, Ahmed Rejeb, Khaled Miled, Adeljelil Ghram, Mehdi Houimel
Summary: This study identified new antiviral peptides that can inhibit the infectivity of H9N2 virus by screening a random linear hexapeptide library. Sixteen different phage-peptides were found to specifically bind to H9N2 virus, and two selected peptides (LSRMPK and FAPRWR) showed antiviral activity in both in ovo and in vivo experiments. These peptides prevent infection by inhibiting the attachment of the H9N2 virus to the cellular receptor.
Review
Chemistry, Multidisciplinary
Golnaz Kamalinia, Brian J. Grindel, Terry T. Takahashi, Steven W. Millward, Richard W. Roberts
Summary: mRNA display is a powerful platform for directed evolution of proteins and peptides, linking phenotype and genotype through puromycin. Iterative selection cycles lead to peptides with desired functional levels. This review discusses the development and broad application of mRNA display technology in selecting novel peptides and proteins.
CHEMICAL SOCIETY REVIEWS
(2021)
Review
Pharmacology & Pharmacy
Ali Akbar Alizadeh, Michael B. Morris, W. Bret Church, Shadi Yaqoubi, Siavoush Dastmalchi
Summary: TNFa plays a crucial role in physiological processes, but excessive levels can contribute to inflammatory and autoimmune diseases. Therefore, researchers have focused on developing strategies to counteract aberrant TNFa concentrations. Promising approaches include destabilizing the active form of TNFa and blocking its interaction with TNFa receptor type I. The use of phage-display technology has been successful in identifying peptides and antibodies that inhibit TNFa activity or prevent its binding to its receptor.
DRUG DISCOVERY TODAY
(2022)
Review
Chemistry, Analytical
Vanessa Escobar, Natale Scaramozzino, Jasmina Vidic, Arnaud Buhot, Raphael Mathey, Carole Chaix, Yanxia Hou
Summary: Foodborne pathogens cause a significant number of illnesses worldwide. In recent decades, there has been a growing development of accurate biosensors for detecting bacterial pathogens in food, using peptides as recognition biomolecules. This review discusses strategies for designing and screening sensitive peptide bioreceptors, as well as advanced techniques for peptide-based biosensors and innovative approaches like electronic noses for food monitoring. These biosensors and electronic noses offer promising alternatives for highly sensitive, cost-effective, and rapid pathogen detection, some of which can be used as portable devices for on-site analysis.
Review
Food Science & Technology
Long Li, Shuangmin Wu, Yu Si, Huaming Li, Xiaoyang Yin, Dapeng Peng
Summary: The single-chain fragment variable (scFv) is a reliable, efficient, and accurate method for analyzing small-molecule harmful substances in food. It offers advantages over traditional antibodies and can be further improved through efficient construction, mutation, and expression measures.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Nanoscience & Nanotechnology
Alexander J. Winton, Mark A. Allen
Summary: Phage display is used to identify polypeptides binding to lithium-ion battery materials. Output/input assays and transmission electron microscopy are employed for quantitative measure and verification. Isothermal titration calorimetry and alanine scanning determine binding residues and affinity, and a bifunctional peptide is validated for stability and potential application in lithium-ion battery electrodes.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Engineering, Biomedical
Qi Chen, Donghui Zhang, Jiawei Gu, Haodong Zhang, Xue Wu, Chuntao Cao, Xinyue Zhang, Runhui Liu
Summary: This study highlights the negative impact of nonspecific protein adsorption and cell adhesion on the surface of biological materials and detecting devices, emphasizing the importance of choosing appropriate antifouling surfaces, structures, and grafting methods. Researchers have continuously studied antifouling strategies to minimize background noise and effectively present the function of bioactive molecules on surfaces.
ACTA BIOMATERIALIA
(2021)
Review
Biochemistry & Molecular Biology
Kangran Zhang, Yezhong Tang, Qin Chen, Yang Liu
Summary: This review discusses the regular process of using phage display technology to screen therapeutic peptides and the peptides that have been screened for their anti-inflammatory properties in recent years. The article describes how these peptides were screened and their in vitro and in vivo effects. It also explores the current challenges and future outlook of using phage display to obtain anti-inflammatory therapeutic peptides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Man-Yeon Choi, Robert K. Vander Meer
Summary: This study introduces a novel GPCR-based screening technology using short peptides generated by bacteriophages and an insect Sf9 cell expression system, presenting a new mechanism called receptor interference (RECEPTORi). This screening method offers advantages such as low cost, rapid results, flexibility in delivery methods, and minimizing the development of insecticide resistance.
Article
Biotechnology & Applied Microbiology
Ivan V. Panayotov, Attila G. Vegh, Marta Martin, Boyan Vladimirov, Christian Larroque, Csilla Gergely, Frederic J. G. Cuisinier, Elias Estephan
Summary: The functionalization of titanium and titanium alloys implant surfaces via material-specific peptides influences host/biomaterial interaction. The use of peptides as molecular linkers between cells and implant material to improve keratinocyte adhesion is reported. In vivo and in vitro experiments demonstrate the promising performance of bioengineered peptides in improving epithelial cell adhesion to titanium-based implants.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Infectious Diseases
Larissa Silva de Freitas, Maria Alice Freitas Queiroz, Luiz Fernando Almeida Machado, Antonio Carlos Rosario Vallinoto, Marluisa de Oliveira Guimaraes Ishak, Fabiana de Almeida Araujo Santos, Luiz Ricardo Goulart, Ricardo Ishak
Summary: Phage display technology was used to explore peptide mimetics of immunoreactive and immunogenic antigens of C. trachomatis, and the peptides showed high sensitivity and AUC values, indicating their potential for screening C. trachomatis infections.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Biochemistry & Molecular Biology
Wenbo Zhou, Julia Craft, Alex Ojemann, Luke Bergen, Arin Graner, Aitana Gonzales, Qianbin He, Timothy Kopper, Marie Smith, Michael W. Graner, Xiaoli Yu
Summary: WHO Grade 4 IDH-wild type astrocytoma (GBM) is a deadly brain tumor with poor prognosis, while meningioma (MMA) is a common benign tumor. This study identified specific peptides using phage display technology to isolate and characterize extracellular vesicles (EVs) in brain tumors, which may have important implications for the treatment of brain tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Obstetrics & Gynecology
Ge Zhao, Jing Liu, Tao Meng
Summary: This meta-analysis identified a significant association between EPHX1 rs1051740 and PE risk in Caucasians, and a statistically significant association between EPHX1 rs2234922 polymorphism and PE in Middle Easterners. No significant association was found between MnSOD rs4880 polymorphism and PE in any genetic models or population groups.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2022)
Review
Obstetrics & Gynecology
Jing Liu, Guang Song, Ge Zhao, Tao Meng
Summary: Magnesium supplementation does not reduce the frequency of leg cramps or improve recovery during pregnancy, and it also does not have significant side effects compared to the control group.
TAIWANESE JOURNAL OF OBSTETRICS & GYNECOLOGY
(2021)
Article
Medicine, General & Internal
Jing Liu, Guang Song, Ge Zhao, Tao Meng
Summary: The study found that excessive weight retention at 6 weeks postpartum after the first pregnancy is associated with an increased risk of large-for-gestational age (LGA) in the subsequent pregnancy, especially for underweight and normal weight women.
Article
Clinical Neurology
Jian Wang, Ge Zhao, Guang Song, Jing Liu
Summary: In this study, TAPB was found to be the most effective local anesthetic technique for postoperative CS pain. Comparing to other techniques, TAPB showed better outcomes in reducing morphine consumption, pain scores, and risks of postoperative nausea, vomiting, and sedation.
JOURNAL OF PAIN RESEARCH
(2021)
Article
Engineering, Chemical
Zilong Liu, Xue Li, Wenhao He, Ge Zhao, Yanning Yang, Xinlei Liu, Xiao Zhang, Xiaofang Li, Suian Zhang, Weichao Sun, Guiwu Lu
Summary: In this study, a novel approach of charge/strain-regulated gas capture and separation was proposed. By using molecular dynamics simulations and first-principle density function theory calculations, the highly controllable CO2 capture and separation on porous g-C9N7 nanosheets were demonstrated. The results showed that the CO2 permeance can be significantly increased by charge engineering and strain engineering, providing useful guidance for developing advanced materials with highly controllable CO2 capture and separation properties.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Environmental Sciences
Yunting Feng, Yong Geng, Ge Zhao, Mengya Li
Summary: Carbon emission reduction is a consensus goal for achieving environmental sustainability, and the use of carbon emission trading policies is considered effective. This paper integrates remanufacturing with carbon emission policies to construct a low-carbon supply chain. The impact of carbon emission constraint policy and carbon market on enterprises' profit and carbon emission reduction is analyzed. The results provide insights for the government and managers to achieve carbon emission reduction without sacrificing economic growth.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Microbiology
Tengteng Yang, Ge Zhao, Yunzhe Liu, Lin Wang, Yubin Gao, Jianmei Zhao, Na Liu, Xiumei Huang, Qingqing Zhang, Junhui Liu, Xiyue Zhang, Junwei Wang, Ying Xu
Summary: A quantitative exposure assessment model for Escherichia coli in swine-slaughtering processes was established to evaluate the risk of pork contamination during slaughtering, identifying the trimming process as the most important key control point.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cell Biology
Yi Zhao, Ge Zhao, Weiwei Li
Summary: The expression of miR-495, p53, and PUMA is increased in placental tissues of pre-eclampsia patients, while HDAC2 expression is decreased. miR-495 negatively regulates HDAC2 expression, leading to the suppression of p53 expression. Overexpression of miR-495, p53, or PUMA inhibits the biological properties of trophoblast cells.
CELL DEATH DISCOVERY
(2022)
Article
Clinical Neurology
Jian Wang, Ge Zhao, Guang Song, Jing Liu
Summary: The study found that neuraxial labor analgesia has a protective effect in reducing the risk of postpartum depression (PPD) in regions with high PPD prevalence, but may increase the risk in regions with low PPD prevalence. Neuraxial labor analgesia did not have a significant impact on postpartum Edinburgh Postpartum Depression Scale scores.
JOURNAL OF AFFECTIVE DISORDERS
(2022)
Article
Biochemical Research Methods
Mantang Chen, Yaqiong Qin, Sheng Wang, Shaofeng Liu, Ge Zhao, Hongliang Lu, Huapeng Cui, Junlan Cai, Xiaoyu Wang, Quanping Yan, Chenfeng Hua, Fuwei Xie, Libin Wan
Summary: This study developed a method to determine the nicotine concentration in inhaled aerosols and successfully applied it for the comparison of heated tobacco products and electronic cigarettes. The method demonstrated high efficiency, selectivity, and sample clean-up capability, with good precision and repeatability.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Obstetrics & Gynecology
Ge Zhao, Guang Song, Jing Liu
Summary: The double-balloon catheter did not show superiority over other methods in cervical ripening and labor induction. However, the combination of double-balloon catheter with oral misoprostol significantly reduced the rate of cesarean section without increased risk.
BMC PREGNANCY AND CHILDBIRTH
(2022)
Article
Microbiology
Ting Wang, Jie Xu, Beibei Wang, Yulian Wang, Wei Zhao, Bin Xiang, Yuhua Xue, Quan Yuan, Yiqiang Wang
Summary: This study identified two peptides using PhD methodology that have the potential to interfere with the interaction between S protein and ACE2. These peptides showed inhibitory effects on the binding of S protein to ACE2 at high concentrations, suggesting their potential as therapeutic agents for blocking the attachment of SARS-CoV-2 virus to host cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Ophthalmology
Yunlan Tang, Enming Du, Gang Wang, Fangyuan Qin, Zhihong Meng, Lijuan Dai, Yiqiang Wang, Shengwei Ren
Summary: This study investigated the role and mechanism of SMAD3 downregulation in TGF-β1-induced corneal fibroblast differentiation. The results showed that SMAD3 expression was dramatically decreased during the process, mainly at the transcription level. Additionally, overexpression or knockdown of SMAD3 enhanced or suppressed TGF-β1-induced fibroblast differentiation, respectively. It was also found that SMAD4 and p38 signaling pathway played a role in regulating SMAD3 downregulation. These findings suggest that targeting SMAD3 may be a potential treatment for corneal fibrosis.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Hematology
Zhenjiang Sun, Dandan Lin, Ying Shen, Kunpeng Ma, Benfang Wang, Hong Liu, Suning Chen, Depei Wu, Yiqiang Wang
Summary: The expression of MXRA7 gene is associated with overall survival of acute myeloid leukemia (AML) patients. MXRA7 regulates cell apoptosis and differentiation, and alters the expression of genes involved in leukemic cell differentiation and growth. MXRA7 also affects the malignancy of NB4 cells.
EXPERIMENTAL HEMATOLOGY
(2023)
Article
Immunology
Ying Shen, Jinling Ning, Lu Zhao, Wei Liu, Ting Wang, Jie Yu, Yiqiang Wang
Summary: MXRA7 is involved in cutaneous wound healing process, and its deficiency impairs wound healing. Lack of MXRA7 impairs the expression of extracellular matrix genes (such as MMP-2) and inhibits signaling pathways (such as STAT3) in healing tissues. In vitro studies also showed that MXRA7 deficiency affects fibroblast migration, contraction, and proliferation. Further investigations revealed that MXRA7 interacts with vimentin and regulates fibroblast functions through signaling pathways like JNK and STAT3/STAT5.
INFLAMMATION AND REGENERATION
(2023)
