Article
Multidisciplinary Sciences
Shunhui Wei, Julian Behn, Charlene Priscilla Poore, See Wee Low, Bernd Nilius, Hao Fan, Ping Liao
Summary: This study designed a mouse monoclonal antibody called M4M to block human TRPM4 channel. Using peptide microarray, a binding epitope EPGF within the A2 peptide was identified. Structural analysis showed that EPGF is located externally to the channel pore and A1 peptide is close to the EPGF binding epitope. Electrophysiological study revealed that M4M can block human TRPM4, but not rodent TRPM4.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, Research & Experimental
Nayanendu Saha, Du-San Baek, Rachelle P. Mendoza, Dorothea Robev, Yan Xu, Yehuda Goldgur, M. Jason De La Cruz, Elisa de Stanchina, Peter W. Janes, Kai Xu, Dimiter S. Dimitrov, Dimitar B. Nikolov
Summary: Metastasis and chemoresistance in colorectal cancer can be inhibited by targeting the substrate-binding region of ADAM10, a metalloprotease that regulates Notch signaling in certain cancer stem cells. A human monoclonal antibody named 1H5 was developed to bind to the substrate-binding domain of ADAM10, inhibiting Notch cleavage and proliferation of colon cancer cells. In mouse models, 1H5 combined with Irinotecan showed highly effective tumor growth inhibition without toxicity. This approach overcomes the limitations of small molecule inhibitors that target the protease active site and exhibit musculoskeletal toxicity.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Virology
Rongyuan Gao, Zhao Wang, Tirth Uprety, Chithra C. C. Sreenivasan, Zizhang Sheng, Ben M. M. Hause, Colin Brunick, Hua Wu, Thomas Luke, Christoph L. L. Bausch, Eddie J. J. Sullivan, Adam D. D. Hoppe, Victor C. C. Huber, Dan Wang, Feng Li
Summary: The Diversitab system utilizes Tc bovines to produce high titer fully human polyclonal IgG immunoglobulins that have been shown to be safe and effective against various virulent pathogens. A human monoclonal antibody, 38C2, isolated from this platform, exhibits ADCC activity in vitro but lacks neutralizing activity against H1N1 virus. Further investigation reveals that 38C2 targets a conserved epitope on H1N1 influenza viruses, suggesting its potential as a therapeutic agent for influenza virus infections.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Virology
Liyan Wang, Fan Yang, Yixin Xiao, Bin Chen, Fumin Liu, Linfang Cheng, Hangping Yao, Nanping Wu, Haibo Wu
Summary: This study obtained three monoclonal antibodies against influenza virus from mice immunized with the HA protein of A/Michigan/45/2015(H1N1), demonstrating strong preventive and therapeutic effects. Two amino acid mutations were discovered, and one of the antibodies still showed protective efficacy after 24 hours of infection.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rui Chen, Yimin Wen, Enbo Yu, Junpeng Yang, Yixiao Liang, Daili Song, Yiping Wen, Rui Wu, Qin Zhao, Senyan Du, Qigui Yan, Xinfeng Han, Sanjie Cao, Xiaobo Huang
Summary: We identified two monoclonal antibodies that effectively neutralized PDCoV, with one targeting the S280-288 epitope and the other targeting the S506-513 epitope. The S280-288 epitope induced specific neutralizing antibodies and provided partial protection against disease in piglets. This study provides valuable insights for understanding the pathogenic mechanism of PDCoV, vaccine design, and antiviral drug development.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Virology
Wanzhi Huang, Victoria Soeung, David M. Boragine, Liya Hu, B. V. Venkataram Prasad, Mary K. Estes, Robert L. Atmar, Timothy Palzkill
Summary: This study utilized a high-resolution method to map multiple antibody binding sites simultaneously from complex serum samples. The results indicated that a relatively small number of sites on the virus bind a large number of independently generated antibodies, suggesting that immunodominance plays a role in the humoral immune response to NoV infections.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hiroyuki Suzuki, Kazuki Ozawa, Tomohiro Tanaka, Mika K. Kaneko, Yukinari Kato
Summary: CD44 is investigated as a cancer stem cell marker in tumor progression. Different splicing variants are overexpressed in many cancers and play critical roles in tumor metastasis, acquisition of stem cell properties, and resistance to treatments. Therefore, understanding the functions and distribution of each CD44 variant is important for novel tumor diagnosis and therapy.
Article
Biotechnology & Applied Microbiology
Yu-Chen Cheng, Shih-Chung Chang
Summary: The study prepared two monoclonal antibodies, 7H6C and YC8, with subtype specificity for H5N8 and H5Nx avian influenza viruses, which can be used for specific detection of HA proteins.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Xiaofen Huang, Yike Li, Meifeng Nie, Mingxi Yue, Yufang Li, Zhijie Lin, Huirong Pan, Mujin Fang, Ting Wu, Shaowei Li, Jun Zhang, Ningshao Xia, Qinjian Zhao
Summary: Thimerosal negatively affects the capsid stability and antigenicity of recombinant HPV VLPs, leading to destabilization of the capsid. The destabilization is dependent on thimerosal concentration, with certain neutralizing antibodies being particularly sensitive to thimerosal treatment. Monitoring antigenicity loss with different antibodies showed distinct kinetic parameters.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2021)
Article
Virology
Justas Lazutka, Karolis Simutis, Paulius Matulis, Rasa Petraityte-Burneikiene, Indre Kucinskaite-Kodze, Martynas Simanavicius, Paulius Lukas Tamosiunas
Summary: The PARV4 virus' VP2 protein can form VLPs in yeast, serving as antigen carriers for vaccine development. This study successfully generated monoclonal antibodies in mice using PARV4 VLPs obtained from yeast, mapping at least three distinct antigenic sites of the VP2 protein, which can advance further research in this area.
Article
Immunology
Suzanne Bezstarosti, Cynthia S. M. Kramer, Marry E. I. Franke-van Dijk, Manon Vergunst, Kim H. Bakker, Merve Uyar-Mercankaya, Rico Buchli, Dave L. Roelen, Johan W. de Fijter, Frans H. J. Claas, Sebastiaan Heidt
Summary: HLA-DQ donor-specific antibodies (DSA) play a significant role in renal transplantation, and this study focuses on isolating HLA-DQ specific memory B cells to generate monoclonal antibodies that can verify the recognition of eplets on HLA molecules. The findings provide valuable insights into the epitopes of HLA-DQ, contributing to the understanding of HLA-DQ alloantibody pathogenicity in transplantation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Yang Qin, Jiayi Jin, Jiani Zhang, Hui Wang, Li Liu, Yanwen Zhang, Sunwang Ling, Jinzhu Hu, Nuan Li, Jianguang Wang, Chen Lv, Xinyu Yang
Summary: Rheumatoid arthritis (RA) is a common chronic autoimmune disease for which traditional treatments often fall short. In this study, researchers developed a fully human SEMA5A-blocking antibody and demonstrated its effectiveness in inhibiting disease progression and improving disease characteristics in a mouse model of RA.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Oncology
Nayanendu Saha, Kai Xu, Zhongyu Zhu, Dorothea Robev, Teja Kalidindi, Yan Xu, Juha Himanen, Elisa de Stanchina, Naga Vara Kishore Pillarsetty, Dimiter S. Dimitrov, Dimitar B. Nikolov
Summary: ADAM17 is overexpressed in various cancers and activates signaling pathways, including EGFR/ErbB, and plays a role in resistance to targeted anti-EGFR therapies. Researchers have identified an inhibitory monoclonal antibody called D8P1C1 that selectively recognizes ADAM17 on cancer cells. This antibody inhibits ADAM17's catalytic activity and the proliferation of different cancer cell lines. In mouse models of breast cancer and ovarian cancer, treatment with D8P1C1 results in significant tumor growth inhibition.
TRANSLATIONAL ONCOLOGY
(2022)
Article
Immunology
Lei Chen, Jiansheng Lu, Junjie Yue, Rong Wang, Peng Du, Yunzhou Yu, Jiazheng Guo, Xi Wang, Yujia Jiang, Kexuan Cheng, Zhixin Yang, Tao Zheng
Summary: A humanized monoclonal antibody 9-8-h2, derived from mice immunization, showed potent neutralization activity against HAdV55. It recognized a conformational neutralization epitope and had favorable physicochemical properties. It could be a promising molecule for the prevention and treatment of HAdV55.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biology
Oi Wah Liew, Samantha S. M. Ling, Shera Lilyanna, Yue Zhou, Peipei Wang, Jenny P. C. Chong, Yan Xia Ng, Angeline E. S. Lim, Eliot R. Y. Leong, Qifeng Lin, Teck Kwang Lim, Qinsong Lin, Enoch M. W. Ng, Tuck Wah Ng, A. Mark Richards
Summary: Liew et al. present an efficient monoclonal antibody (mAb) production method, successfully developing mAbs against multiple epitopes on hANKRD1 in a single hybridoma production cycle. With rapid screening and validation techniques, this pipeline shows promise of general applicability for antibody panel generation.
COMMUNICATIONS BIOLOGY
(2021)
Article
Surgery
Naoya Yoshida, Keisuke Kosumi, Ryuma Tokunaga, Baba Yoshifumi, Yohei Nagai, Yuji Miyamoto, Shiro Iwagami, Masaaki Iwatsuki, Yukiharu Hiyoshi, Takatsugu Ishimoto, Kojiro Eto, Yu Imamura, Masayuki Watanabe, Hideo Baba
Article
Oncology
Naoya Yoshida, Yuki Adachi, Takeshi Morinaga, Kojiro Eto, Ryuma Tokunaga, Kazuto Harada, Yukiharu Hiyoshi, Yohei Nagai, Masaaki Iwatsuki, Takatsugu Ishimoto, Yoshifumi Baba, Shiro Iwagami, Yuji Miyamoto, Yu Imamura, Masayuki Watanabe, Hideo Baba
ANNALS OF SURGICAL ONCOLOGY
(2020)
Article
Oncology
Kazuo Okadome, Yoshifumi Baba, Daichi Nomoto, Taisuke Yagi, Rebecca Kalikawe, Kazuto Harada, Yukiharu Hiyoshi, Yohei Nagai, Takatsugu Ishimoto, Masaaki Iwatsuki, Shiro Iwagami, Yuji Miyamoto, Naoya Yoshida, Masayuki Watanabe, Yoshihiro Komohara, Takashi Shono, Yutaka Sasaki, Hideo Baba
BRITISH JOURNAL OF CANCER
(2020)
Review
Oncology
Mari Hosonaga, Hideyuki Saya, Yoshimi Arima
CANCER AND METASTASIS REVIEWS
(2020)
Article
Oncology
Yoshimi Arima, Hiroyuki Nobusue, Hideyuki Saya
Article
Virology
Masaru Takeshita, Naoshi Nishina, Saya Moriyama, Yoshimasa Takahashi, Yoshifumi Uwamino, Mika Nagata, Wataru Aoki, Katsunori Masaki, Makoto Ishii, Hideyuki Saya, Yasushi Kondo, Yuko Kaneko, Katsuya Suzuki, Koichi Fukunaga, Tsutomu Takeuchi
Summary: The study found that asymptomatic and mild COVID-19 patients had inadequate humoral immune responses, with most antibodies being correlated with disease severity, symptoms such as pneumonia, lymphopenia, and certain serological markers, and some patients failing to develop antibodies against the virus.
Article
Biochemistry & Molecular Biology
Toshikatsu Tsuji, Yusuke Maeda, Kenji Kita, Kazuhiro Murakami, Hideyuki Saya, Hirofumi Takemura, Noriyuki Inaki, Masanobu Oshima, Hiroko Oshima
Summary: The study found that FOXO3 displays different expression patterns in different subtypes of gastric cancer: in FOXO3-Cyt gastric cancer cells, activation of mutant FOXO3 leads to nuclear accumulation and growth inhibition, while inhibition of the PI3K-AKT pathway also suppresses proliferation in this type of gastric cancer cells.
Article
Oncology
Ryo Sato, Kosuke Imamura, Takashi Semba, Yusuke Tomita, Sho Saeki, Koei Ikeda, Yoshihiro Komohara, Makoto Suzuki, Takuro Sakagami, Hideyuki Saya, Yoshimi Arima
Summary: CAF secrete TGF beta to induce a solid-to-acinar transition in lung cancer cells, demonstrating how the tumor microenvironment influences histological patterns and tumor heterogeneity in lung adenocarcinoma.
Review
Biochemistry & Molecular Biology
Sana Hibino, Tetsuro Kawazoe, Hidenori Kasahara, Shinji Itoh, Takatsugu Ishimoto, Mamiko Sakata-Yanagimoto, Koji Taniguchi
Summary: Inflammation plays a pivotal role in tumorigenesis and metastasis by suppressing anti-tumor immunity. Different types of tumors may have diverse responses to inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell & Tissue Engineering
Go J. Yoshida, Hideyuki Saya
Summary: Intratumoral heterogeneity is closely linked to treatment failure in cancer, with cancer stem cells (CSCs) playing a key role in this process. CSCs, located at the top of the tumor cell hierarchy, possess self-renewal and therapeutic resistance capabilities, contributing to the challenges in cancer therapy.
REGENERATIVE THERAPY
(2021)
Article
Biology
Sayaka Ueno, Tamotsu Sudo, Hideyuki Saya, Eiji Sugihara
Summary: This study reveals that pigment epithelium-derived factor (PEDF) promotes the dissemination of ovarian cancer by inducing IL-10 expression in macrophages. High PEDF gene expression is associated with poor prognosis in ovarian cancer patients, and elevated PEDF levels are correlated with early recurrence. BET inhibitors reduce PEDF expression and limit both cancer cell survival and macrophage induction.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Haruki Inoue, Eriko Aimono, Akiyoshi Kasuga, Haruto Tanaka, Aika Iwasaki, Hideyuki Saya, Yoshimi Arima
Summary: Our mouse BTC models are suitable for studying BTC carcinogenesis and may contribute to the development of new therapeutic strategies. Analysis of images of mouse and human tumor tissues reveals similarities in tissue structure between the two, suggesting similarities in tumor characteristics independent of animal species. Additionally, our pixel-level clustering model can serve as a new diagnostic tool for BTC.
Article
Medicine, Research & Experimental
Tomofumi Ando, Ikue Tai-Nagara, Yuki Sugiura, Dai Kusumoto, Koji Okabayashi, Yasuaki Kido, Kohji Sato, Hideyuki Saya, Sutip Navankasattusas, Dean Y. Li, Makoto Suematsu, Yuko Kitagawa, Elena Seiradake, Satoru Yamagishi, Yoshiaki Kubota
Summary: Blood vessel abnormalities in cancer can be targeted therapeutically to suppress cancer progression, according to a study. Researchers found that a molecule called fibronectin leucine-rich transmembrane protein 2 (FLRT2) is expressed in abnormal blood vessels of advanced colorectal cancers in humans. Deleting FLRT2 in endothelial cells selectively pruned abnormal blood vessels and suppressed tumor metastasis. Additionally, FLRT2 deletion increased the number of mature vessels, enhancing the antitumor effects of immune checkpoint blockers. The study suggests that targeting tumor-specific interendothelial adhesions could be a safe and effective therapeutic option.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Surgery
Tadahito Yasuda, Kojiro Eto, Naoya Yoshida, Shiro Iwagami, Yukiharu Hiyoshi, Youhei Nagai, Masaaki Iwatsuki, Takatsugu Ishimoto, Yoshifumi Baba, Yuji Miyamoto, Takuya Shiota, Yoshiki Mikami, Hideo Baba
SURGICAL CASE REPORTS
(2020)
Review
Biochemistry & Molecular Biology
Lingfeng Fu, Luke Bu, Tadahito Yasuda, Mayu Koiwa, Takahiko Akiyama, Tomoyuki Uchihara, Hideo Baba, Takatsugu Ishimoto