Article
Cell Biology
Guanlin Wu, Weidong Weng, Pengfei Xia, Shixian Yan, Cheng Zhong, Lei Xie, Yu Xie, Gang Fan
Summary: This review aims to summarize previous studies related to bladder cancer and the Wnt signaling pathway, with a focus on exploring the participating substances and their mechanisms in the regulation of the Wnt signaling pathway to better determine how to promote new chemotherapeutic drugs, potential therapeutic targets, and diagnostic biomarkers.
CELLULAR SIGNALLING
(2021)
Article
Biochemistry & Molecular Biology
Jochen Neuhaus, Annett Weimann, Mandy Berndt-Paetz
Summary: This study demonstrates time-dependent interactions of endogenous Wnt receptors in PC-3 cells, leading to rapid activation of Wnt/beta-catenin and Wnt/PCP pathways following stimulation by Wnt ligands. The proximity ligation assay (PLA) provides novel insights into Wnt receptor activation in mammalian cells and may uncover new signaling routes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Jiajia Wang, Xichen Wan, Qihua Le
Summary: SOX9, a member of the SRY-related HMG-box transcription factors, plays a critical role in fetal development and stem cell homeostasis. The interactions between SOX9 and the canonical Wnt signalling pathway are involved in various physiological and pathological processes of stem cells, including organ development, stem cell proliferation, differentiation, and tumorigenesis. This review summarizes the molecular mechanism of cross-interactions between SOX9 and the canonical Wnt signalling pathway, outlines its regulatory effects on homeostasis in different types of stem cells, and explores its potential in translational stem cell therapy.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Review
Oncology
Siwei Yu, Ruyue Han, Runliang Gan
Summary: This review aims to analyze the mechanisms of action of related molecules in the Wnt/beta-catenin pathway in hematologic malignancies and explore their feasibility as therapeutic targets.
BIOMARKER RESEARCH
(2022)
Article
Cell & Tissue Engineering
Kai Hang, Li Ying, Jinwu Bai, Yibo Wang, Zhihui Kuang, Deting Xue, Zhijun Pan
Summary: The study showed that the silencing of SERPINB2 can promote osteogenic differentiation of BMSCs through the Wnt/β-catenin signaling pathway. In vivo experiments demonstrated that silencing SERPINB2 effectively improves bone fracture healing, suggesting that SERPINB2 may be a novel target for bone fracture healing.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell Biology
Yaoyao Zhu, Xia Li
Summary: Colorectal cancer is a common and deadly cancer worldwide, with a close association to genetic mutations, chronic inflammatory disorders, and poor dietary habits. The Wnt signaling pathway plays a crucial role in various physiological processes of colorectal cancer. This review summarizes the recent advances in understanding the mechanisms and potential regulators of the three branches of the Wnt signaling pathway in colorectal cancer.
Article
Cell Biology
Xiaoxi Yang, Chuiguo Sun, Xiangyu Meng, Guanghui Chen, Tianqi Fan, Chi Zhang, Zhongqiang Chen
Summary: This study revealed the differential gene expression profile associated with LGR and Wnt signaling pathway in thoracic ligamentum flavum cells (TLFCs) during different stages of ossification through RNA sequencing and gene ontology enrichment analysis. The findings suggest that LGR5 enhances osteogenesis by activating the Wnt signaling pathway in thoracic ossification of the ligamentum flavum (TOLF).
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Editorial Material
Cell Biology
Yang Yang, Eileen White
Summary: Macroautophagy/autophagy defects are a risk factor for inflammatory bowel disease. Studies have shown that the deletion of autophagy genes can lead to the loss of intestinal stem cells and barrier function, contributing to the development of IBD.
Article
Cell Biology
Yuan Li, Jilong Zou, Bo Li, Jianyang Du
Summary: This study found that melatonin inhibits the biological functions of osteosarcoma (OS) cells by repressing the expression of lncRNA JPX and regulating the Wnt/beta-catenin signaling pathway, suggesting a potential therapeutic strategy for OS.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Hyun-Yi Kim, Shujin Li, Dong-Joon Lee, Jin Hoo Park, Takashi Muramatsu, Hidemitsu Harada, Young-Soo Jung, Han-Sung Jung
Summary: A potential cancer stem cell population was identified in ameloblastoma cells, and the activation of Wnt signaling pathway was found to be a promising therapeutic approach to decrease this population.
CELL PROLIFERATION
(2021)
Article
Biochemistry & Molecular Biology
Roland Kotolloshi, Mieczyslaw Gajda, Marc-Oliver Grimm, Daniel Steinbach
Summary: The study revealed that BCL9L and Wnt/beta-catenin signaling play an oncogenic role in bladder cancer cells, and may be associated with the progression of bladder cancer. Mutations in UTRs of BCL9L can affect its activity, thereby influencing cell proliferation, migration, and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Yun Li, Chenying Liu, Xin Zhang, Xiaodi Huang, Shujun Liang, Feiyue Xing, Han Tian
Summary: CCT5 is upregulated in gastric cancer with lymph node metastasis and is associated with poor prognosis. CCT5 promotes cell proliferation, invasion, and lymphatic tube formation in gastric cancer. It also enhances tumor growth and lymph node metastasis in vivo. By disrupting the interaction between E-cadherin and beta-catenin, CCT5 enhances Wnt/beta-catenin signaling activity and epithelial-mesenchymal transition. These findings suggest that CCT5 may serve as a potential biomarker for the diagnosis and treatment of gastric cancer.
BRITISH JOURNAL OF CANCER
(2022)
Article
Cell Biology
Huaji Jiang, Jialiang Zhong, Wenjun Li, Jianghui Dong, Cory J. Xian, Yung-Kang Shen, Lufeng Yao, Qiang Wu, Liping Wang
Summary: The study found that GEN can promote osteogenesis of BMSCs by activating the BMP and Wnt/β-catenin pathways, providing a novel strategy for the treatment of osteoporosis.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Yanan Yang, Guodong Deng, Lili Qiao, Hui Yuan, Xiaohong Yu, Lei Xu, Shih-Hsin Lu, Wei Jiang, Xiying Yu
Summary: This study identified a slow-cycling/quiescent stem cell population with high levels of hemidesmosomes (HDs) and low levels of Wnt signaling in the basal layer of the esophageal epithelium, and revealed that HDs and Wnt signaling are critical determinants for defining the stem cells of the basal layer required for tissue homeostasis in mammalian esophagi.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Cell Biology
Tapan Sharma, Monserrat Olea-Flores, Anthony N. Imbalzano
Summary: Skeletal muscle differentiation is regulated by the mSWI/SNF chromatin remodeling family, which also plays a role in the regulation of the Wnt signaling pathway. Knockdown experiments showed that BRG1 is a regulator of Wnt pathway components, and reactivation of the pathway rescued defects in myogenic gene expression caused by BRG1 knockdown. Chromatin immunoprecipitation provided mechanistic insight into the transcriptional regulation of Wnt pathway genes by BRG1 and beta-catenin.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Engineering, Mechanical
JinJing Liao, David W. Smith, Saeed Miramini, Bruce S. Gardiner, Lihai Zhang
Summary: This study developed a multiscale cartilage contact model to investigate the effect of the polymer brush border (PBB) on fluid permeability and lubrication in cartilage. The results showed that PBB can enhance cartilage lubrication by increasing gap fluid load support, reduce fluid flow resistance, and minimize vertical deformation of the tissue. Additionally, reducing PBB thickness was found to impair cartilage lubrication ability.
Article
Cell Biology
Christoph Grohmann, Francesca Walker, Mark Devlin, Meng-Xiao Luo, Anderly C. Chueh, Judy Doherty, Francois Vaillant, Gwo-Yaw Ho, Matthew J. Wakefield, Clare E. Weeden, Alvin Kamili, Jayne Murray, Sela T. Po'uha, Janet Weinstock, Serena R. Kane, Maree C. Faux, Esmee Broekhuizen, Ye Zheng, Kristy Shield-Artin, Nadia J. Kershaw, Chin Wee Tan, Helen M. Witchard, Gregor Ebert, Susan A. Charman, Ian Street, Maria Kavallaris, Michelle Haber, Jamie I. Fletcher, Marie-Liesse Asselin-Labat, Clare L. Scott, Jane E. Visvader, Geoffrey J. Lindeman, Keith G. Watson, Antony W. Burgess, Guillaume Lessene
Summary: Targeting cell division by the new small molecule WEHI-7326 shows promising anti-tumor activity with broad-spectrum efficacy, providing a potential new therapeutic option for cancer patients.
CELL DEATH & DISEASE
(2021)
Article
Computer Science, Interdisciplinary Applications
JinJing Liao, David W. Smith, Saeed Miramini, Bruce S. Gardiner, Lihai Zhang
Summary: Using a probabilistic approach, this study investigates the influence of surficial cartilage glycoaminoglycan (GAG) content on joint lubrication behavior. The increase of polymer brush border (PBB) thickness enhances hydrodynamic lubrication in the cartilage contact gap, while increasing gap asperity stiffness impairs lubrication. The lubrication performance of cartilage varies with physiological conditions, making the outcomes on joint lubrication difficult to predict.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2021)
Article
Oncology
Christelle Adolphe, Amanda Millar, Marija Kojic, Deborah S. Barkauskas, Anders Sundstrom, Fredrik J. Swartling, Soroor Hediyeh-Zadeh, Chin Wee Tan, Melissa J. Davis, Laura A. Genovesi, Brandon J. Wainwright
Summary: Research reveals three distinct populations of SOX9 in medulloblastoma, with SOX9 potentially playing a key role in SHH-MB initiation in GCP cells. However, SOX9 is not essential for tumorigenesis in medulloblastoma.
MOLECULAR CANCER RESEARCH
(2021)
Article
Biology
Yumiko Hirokawa, Jordan Clarke, Michelle Palmieri, Tao Tan, Dmitri Mouradov, Shan Li, Cong Lin, Fuqiang Li, Huijuan Luo, Kui Wu, Maree Faux, Chin Wee Tan, Margaret Lee, Grace Gard, Peter Gibbs, Antony W. Burgess, Oliver M. Sieber
Summary: The low-viscosity matrix (LVM) suspension culture method allows efficient establishment and propagation of human large intestine organoids and tumoroids, recapitulating morphological development and enabling high-throughput drug sensitivity testing for tumoroids.
COMMUNICATIONS BIOLOGY
(2021)
Article
Respiratory System
Arutha Kulasinghe, Chin Wee Tan, Anna Flavia Ribeiro Dos Santos Miggiolaro, James Monkman, Habib SadeghiRad, Dharmesh D. Bhuva, Jarbas da Silva Motta Junior, Caroline Busatta Vaz de Paula, Seigo Nagashima, Cristina Pellegrino Baena, Paulo Souza-Fonseca-Guimaraes, Lucia de Noronha, Timothy McCulloch, Gustavo Rodrigues Rossi, Caroline Cooper, Benjamin Tang, Kirsty R. Short, Melissa J. Davis, Fernando Souza-Fonseca-Guimaraes, Gabrielle T. Belz, Ken O'Byrne
Summary: Spatial transcriptomics is a powerful tool to identify novel gene signatures within tissues, offering new insights into the pathogenesis of SARS-COV-2 to aid in patient triage and treatment. This study used targeted transcriptomics of formalin-fixed paraffin-embedded tissue to examine the pulmonary transcriptional landscape of COVID-19 patients. The results showed significant upregulation of genes associated with inflammation, type I interferon production, coagulation, and angiogenesis in the lungs of COVID-19 patients compared to non-infected controls. Additionally, the study found that the gene IFI27, previously identified as a useful blood biomarker for differentiating bacterial and viral lung infections, was significantly upregulated in the lungs of COVID-19 patients compared to patients with influenza.
EUROPEAN RESPIRATORY JOURNAL
(2022)
Editorial Material
Cell Biology
Jun T. Low, Gwo-Yaw Ho, Mark Scott, Chin Wee Tan, Lachlan Whitehead, Kathy Barber, Hon Y. K. Yip, Johanna F. Dekkers, Yumiko Hirokawa, John Silke, Antony W. Burgess, Andreas Strasser, Tracy L. Putoczki, Lorraine A. O'Reilly
CELL DEATH & DISEASE
(2021)
Article
Multidisciplinary Sciences
Rachel M. Koldej, Ashvind Prabahran, Chin Wee Tan, Ashley P. Ng, Melissa J. Davis, David S. Ritchie
Summary: HCL is a rare B cell malignancy characterized by hairy B cells and extensive BM infiltration. Treatment with CDA leads to variable response duration. Analysis of the BM microenvironment identified prognostic biomarkers of response to CDA, with dysregulated expression of immune markers pre- and post-CDA treatment. Duration of response was associated with reduction in tumor burden and increased CD8 T cell infiltration into the BM post-CDA.
SCIENTIFIC REPORTS
(2021)
Review
Medicine, Research & Experimental
David W. Smith, Azin Azadi, Chang-Joon Lee, Bruce S. Gardiner
Summary: This review focuses on the spatial location and dynamics of the main extracellular matrix components of the glomerular basement membrane (GBM). It explains the slow dynamic disequilibrium of collagen IV and laminin content, leading to GBM thickening, as well as the rapid turnover of heparan sulfate proteoglycans against the flow of filtrate. The highly rapid turnover of heparan sulfate may serve various functions, such as unclogging the GBM, providing compressive stiffness, and facilitating cell-cell communication.
Article
Immunology
Arutha Kulasinghe, Ning Liu, Chin Wee Tan, James Monkman, Jane E. Sinclair, Dharmesh D. Bhuva, David Godbolt, Liuliu Pan, Andy Nam, Habib Sadeghirad, Kei Sato, Gianluigi Li Bassi, Ken O'Byrne, Camila Hartmann, Anna Flavia Ribeiro Dos Santos Miggiolaro, Gustavo Lenci Marques, Lidia Zytynski Moura, Derek Richard, Mark Adams, Lucia de Noronha, Cristina Pellegrino Baena, Jacky Y. Suen, Rakesh Arora, Gabrielle T. Belz, Kirsty R. Short, Melissa J. Davis, Fernando Souza-Fonseca Guimaraes, John F. Fraser
Summary: The study reveals distinct transcriptomic profiles in cardiac tissues of SARS-CoV-2 and pH1N1 influenza infection, with upregulation of genes associated with DNA damage and repair, heat shock, and macrophage infiltration in COVID-19 patients' cardiac tissues. In comparison, pH1N1 infection showed upregulation of interferon and complement pathways. This highlights the need for further understanding of the effects on extra-pulmonary organs, including the cardiovascular system, in COVID-19 patients.
Article
Immunology
Maryam Shojaei, Amir Shamshirian, James Monkman, Laura Grice, Minh Tran, Chin Wee Tan, Siok Min Teo, Gustavo Rodrigues Rossi, Timothy R. McCulloch, Marek Nalos, Maedeh Raei, Alireza Razavi, Roya Ghasemian, Mobina Gheibi, Fatemeh Roozbeh, Peter D. Sly, Kirsten M. Spann, Keng Yih Chew, Yanshan Zhu, Yao Xia, Timothy J. Wells, Alexandra Cristina Senegaglia, Carmen Lucia Kuniyoshi, Claudio Luciano Franck, Anna Flavia Ribeiro dos Santos, Lucia de Noronha, Sepideh Motamen, Reza Valadan, Omolbanin Amjadi, Rajan Gogna, Esha Madan, Reza Alizadeh-Navaei, Liliana Lamperti, Felipe Zuniga, Estefania Nova-Lamperti, Gonzalo Labarca, Ben Knippenberg, Velma Herwanto, Ya Wang, Amy Phu, Tracy Chew, Timothy Kwan, Karan Kim, Sally Teoh, Tiana M. Pelaia, Win Sen Kuan, Yvette Jee, Jon Iredell, Ken O'Byrne, John F. Fraser, Melissa J. Davis, Gabrielle T. Belz, Majid E. Warkiani, Carlos Salomon Gallo, Fernando Souza-Fonseca-Guimaraes, Quan Nguyen, Anthony Mclean, Arutha Kulasinghe, Kirsty R. Short, Benjamin Tang
Summary: This study found that the IFI27 gene is associated with COVID-19 infection and that its expression levels in the respiratory tract and blood are correlated with viral load and infection severity. IFI27 gene family biomarkers may supplement conventional diagnostic tools in future virus pandemics.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Computer Science, Interdisciplinary Applications
Qin Li, Saeed Miramini, David W. Smith, Bruce S. Gardiner, Lihai Zhang
Summary: This study aims to develop a numerical model to investigate the effects of osteochondral junction leakage on cartilage lubrication. The results show that the permeability of the junction has a significant impact on cartilage lubrication, especially in the early stage of the disease. Increased leakage leads to a decrease in lubrication duration, shorter consolidation time, and an increase in the initial friction coefficient. These findings contribute to a better understanding of the development mechanism of cartilage disorders.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2023)
Article
Physiology
Chang-Joon Lee, Bruce S. Gardiner, Roger G. Evans, David W. Smith
Summary: A computational model of oxygen exchange between urine bolus and ureteral tissue suggests that it may be possible to predict renal medullary Po-2 based on measurement of bladder urine Po-2, with bladder urine Po-2 strongly dependent on bolus volume. Changes in peristaltic frequency had minimal impact on bladder urine Po-2.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Meeting Abstract
Oncology
Shikhar Sharma, Jay Chung, Sean Uryu, Amanda Rickard, Natalie Nady, Showkhin Khan, Zhenxiong Wang, Yong Zhang, Haikuo Zhang, Pei-Pei Kung, Eric Greenwald, Karen Maegley, Patrick Bingham, Hieu Lam, Ylva E. Bozikis, Hendrik Falk, Elizabeth Allan, Vicky M. Avery, Miriam S. Butler, Michelle A. Camerino, Catalina Carrasco-Pozo, Susan A. Charman, Melissa J. Davis, Mark A. Dawson, Dawson Sarah-Jane, Melanie de Silva, Matthew L. Dennis, Olan Dolezal, Rachel Lagiakos, Geoffrey J. Lindeman, Laura MacPherson, Stewart Nuttall, Thomas S. Peat, Bin Ren, Alexandra E. Stupple, Elliot Surgenor, Chin Wee Tan, Tim Thomas, Jane E. Visvader, Anne K. Voss, Francois Vaillant, Karen L. White, James Whittle, Yuqing Yang, Soroor Hediyeh-Zadeh, Paul A. Stupple, Ian P. Street, Brendon J. Monahan, Thomas Paul