Article
Microbiology
Yue Ma, Yingying Yue, Haihong Jia, Nannan Song, Li Zhai, Weiwei Wang, Cuiling Li, Bingqing Li
Summary: This study reveals that YdiU, an UMPylator, plays a critical role in regulating flagellar biogenesis in Salmonella by UMPylating the FlhC subunit of FlhDC and inhibiting its binding to flagellar genes, thus promoting immune escape.
Article
Biochemistry & Molecular Biology
So Yeon Cho, Han Byeol Oh, Sung-il Yoon
Summary: Bacterial flagella assembly is regulated by different proteins in a defined order, and in certain bacteria classes, it is controlled by the master regulator FlhDC. The crystal structure of FlhDC from Cupriavidus necator was determined, revealing its DNA-binding capacity and structural features. This study provides insights into the DNA-binding mechanism and functional differences of FlhDC in different bacteria classes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Guanglu Sun, Zihao Yu, Qianwen Li, Yuanxing Zhang, Mingxiao Wang, Yunhui Liu, Jinze Liu, Lei Liu, Xuping Yu
Summary: Overexpression of the flhDC genes in Escherichia coli can lead to cell death due to excessive production of reactive oxygen species (ROS) from the oxidative phosphorylation pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Melissa Berni, Luca Bolzoni, Ilaria Menozzi, Alessandra Dodi, Chiara Bracchi, Marina Morganti, Erika Scaltriti, Stefano Pongolini, Martina Tambassi
Summary: This study reveals that a significant portion of the global population of Salmonella Derby is evolving to adapt to the swine host while simultaneously attenuating its virulence for humans. The fixation of deleterious mutations in the SPI-1 genes plays a key role in this process. The study provides insights into the molecular mechanisms underlying the ecology and host adaptation of Salmonella.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Hsin-Hung Lin, Hsiu-Ling Chen, Chang-Ching Weng, Rajendra Prasad Janapatla, Chyi-Liang Chen, Cheng-Hsun Chiu
Summary: The study revealed that Salmonella induces apoptosis in macrophages through caspase-3 activation, especially via the expression of SPI-1 structure proteins and effectors. This mechanism may help explain why Salmonella infections in children often present as mild bacteremia with less systemic inflammatory response.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Microbiology
Xiaojing Fan, Zhiwen Zhao, Tingyan Sun, Wei Rou, Caiying Gui, Tahira Saleem, Xuxu Zhao, Xianyuan Xu, Tao Zhuo, Xun Hu, Huasong Zou
Summary: The study reveals that the regulator CrgA in Ralstonia solanacearum suppresses cell motility by repressing flhDC expression, which consequently affects the expression of fliC to modulate disease development in host plants. This indicates a complex regulation of motility in R. solanacearum and enhances understanding of flagellum regulation in this pathogen.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hongou Wang, Zhiheng Tang, Baoshuai Xue, Qinghui Lu, Xiaoyun Liu, Qinghua Zou
Summary: This study reveals the crucial role of STM0347 in Salmonella virulence and flagellar phase variation and highlights the complexity of the regulatory network of Hin-modulated flagellum phase variation in Salmonella.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Jessica Maldonado, Barbara Czarnecka, Dana E. Harmon, Cristian Ruiz
Summary: Efflux and motility are two key biological functions in bacteria. Recent findings have shown that AcrR, a transcriptional repressor, directly regulates motility in E. coli by repressing the expression of flhDC, which is the master regulator of flagellum biosynthesis and motility genes.
Article
Immunology
Jaejin Lee, Eunkyoung Shin, Ji-Hyun Yeom, Jaeyoung Park, Sunwoo Kim, Minho Lee, Kangseok Lee
Summary: This study demonstrates that the expression levels of STRraA, a protein regulator of STRNase E activity, affect Salmonella enterica serovar Typhimurium pathogenicity by protecting fliDST mRNA from STRNase E cleavage, leading to enhanced flagellar assembly.
MICROBIAL PATHOGENESIS
(2022)
Article
Veterinary Sciences
Jwerlly Tatiana Pico-Rodriguez, Hugo Martinez-Jarquin, Jose de Jesus Gomez-Chavez, Mireya Juarez-Ramirez, Luary Carolina Martinez-Chavarria
Summary: The study aimed to evaluate the effect of deleting SPI-1 or SPI-2 genes on intestinal and systemic salmonellosis using the avian model. The results showed that the deletion of these genes attenuated the virulence and reduced the survival ability of bacteria in the intestine and liver.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Junxiang Yan, Qian Liu, Xinke Xue, Jinghao Li, Yuehua Li, Yingying Su, Boyang Cao
Summary: This study reveals that the VC1795 gene plays a key role in the intestinal colonization and pathogenicity of Vibrio cholerae, and it regulates the expression of its downstream gene, VC1794, and the toxin-coregulated pilus (TCP) cluster. These findings provide insights into the regulatory mechanisms of VC1795 in bacterial pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Haihong Jia, Nannan Song, Yue Ma, Fengyu Zhang, Yingying Yue, Weiwei Wang, Cuiling Li, Hui Li, Qi Wang, Lichuan Gu, Bingqing Li
Summary: This study reveals a novel regulation process of iron acquisition mediated by UMPylator YdiU, which inhibits the binding of Fur to target DNA and activates the expression of iron uptake genes. This process is crucial for Salmonella survival within host cells and evasion of host nutritional immunity.
Article
Microbiology
Nancy Leon-Montes, Jessica Nava-Galeana, Diana Rodriguez-Valverde, Jorge Soria-Bustos, Roberto Rosales-Reyes, Sandra Rivera-Gutierrez, Hidetada Hirakawa, Miguel A. Ares, Victor H. Bustamante, Miguel A. De la Cruz
Summary: The CpxRA system represses the expression of SPI-2 genes by directly acting on the ssrAB regulatory operon, and it decreases the replication of Salmonella typhimurium inside macrophages.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Nikhil K.c, Laxmi Noatia, Swagatika Priyadarsini, M. Pashupathi, Jagan Mohanarao Gali, M. Ayub Ali, S. K. Behera, Bhaskar Sharma, Parimal Roychoudhury, Ajay Kumar, Parthasarathi Behera
Summary: Recoding of the anaerobic regulator gene fnr in Salmonella Typhimurium significantly compromises its growth, motility, biofilm forming ability, and survival within macrophages. Additionally, recoding reduces the colonization ability and fecal shedding of the mutant strain in mice. These findings highlight the attenuated pathogenicity of Salmonella Typhimurium after recoding the global anaerobic regulator gene fnr.
MICROBIAL PATHOGENESIS
(2022)
Article
Immunology
Shuangshuang Ma, Lingyan Jiang, Jingting Wang, Xiaoqian Liu, Wanwu Li, Shuai Ma, Lu Feng
Summary: Salmonella enterica serovar Typhimurium utilizes host macrophages as a niche for survival and replication, controlling the expression of flagellar genes to minimize host immune response. The transcriptional regulator AsiR positively regulates flagellar gene expression and its downregulation contributes to S. Typhimurium replication and systemic infection. Acidic pH in macrophages serves as a signal for AsiR and flagellar gene downregulation.
Article
Immunology
Colleen R. Eade, Chien-Che Hung, Brian Bullard, Geoffrey Gonzalez-Escobedo, John S. Gunn, Craig Altier
INFECTION AND IMMUNITY
(2016)
Article
Biochemistry & Molecular Biology
Chien-Che Hung, Colleen R. Eade, Craig Altier
MOLECULAR MICROBIOLOGY
(2016)
Article
Biochemistry & Molecular Biology
Chien-Che Hung, Cherilyn D. Garner, James M. Slauch, Zachary W. Dwyer, Sara D. Lawhon, Jonathan G. Frye, Michael McClelland, Brian M. M. Ahmer, Craig Altier
MOLECULAR MICROBIOLOGY
(2013)
Article
Immunology
Colleen R. Eade, Lydia Bogomolnaya, Chien-Che Hung, Michael I. Betteken, L. Garry Adams, Helene Andrews-Polymenis, Craig Altier
INFECTION AND IMMUNITY
(2019)
Article
Microbiology
Chien-Che Hung, Colleen R. Eade, Michael Betteken, Pau Lina D. Pavinski Bitar, Elaine M. Handley, Staci L. Nugent, Rimi Chowdhury, Craig Altier