Article
Environmental Sciences
Huaqing Liu, Hanzhi Lin, Benru Song, Xiaoxu Sun, Rui Xu, Tianle Kong, Fuqing Xu, Baoqin Li, Weimin Sun
Summary: This study investigated active aniline degraders in different pH environments, revealing that aniline degradation is associated with different bacterial taxa. By comparing three aniline-contaminated cultures, it was found that aniline-degrading bacteria employ diverse survival strategies and exhibit different characteristics in biotic interactions under different pH environments.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemical Research Methods
Pan Deng, Taylor Valentino, Michael D. Flythe, Hunter N. B. Moseley, Jacqueline R. Leachman, Andrew J. Morris, Bernhard Hennig
Summary: By studying metabolites produced by the gut microbiome, differences in the utilization of different dietary fibers for biosynthesis of essential molecules were observed, providing a new method for a deeper understanding of the metabolic function of the gut microbiome.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Engineering, Environmental
Haonan Fan, Shanghua Wu, Wenxu Dong, Xianglong Li, Yuzhu Dong, Shijie Wang, Yong-Guan Zhu, Xuliang Zhuang
Summary: This study identified antibiotic-resistant bacteria in soil-plant systems using stable isotope probing and highlighted the rhizosphere as a hotspot for antibiotic resistance gene transfer.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Microbiology
Alex Greenlon, Ella Sieradzki, Olivier Zablocki, Benjamin J. Koch, Megan M. Foley, Jeffrey A. Kimbrel, Bruce A. Hungate, Steven J. Blazewicz, Erin E. Nuccio, Christine L. Sun, Aaron Chew, Cynthia-Jeanette Mancilla, Matthew B. Sullivan, Mary Firestone, Jennifer Pett-Ridge, Jillian F. Banfield
Summary: The growth and physiology of soil microorganisms are influenced by both current and historical soil environmental conditions. By using genome-resolved metagenomics, we found that precipitation levels have a significant impact on the activity and growth rates of soil microorganisms. In addition, the activity of bacteriophages in the soil is correlated with the activity of their hosts. Contrary to expectations, the ability to decompose organic matter and oxidize carbon compounds is broadly distributed among active and inactive bacteria.
Article
Environmental Sciences
Shuaimin Chen, Eiko E. Kuramae, Zhongjun Jia, Binbin Liu
Summary: This study utilized stable isotope probing and molecular biology techniques to investigate the composition and function of active denitrifying microorganisms in the vadose zone. It revealed significant differences in the composition and function of active denitrifiers between different soil layers. This research improves our understanding of active denitrifying microbes in the vadose zone and provides insights for reducing nitrate pollution in groundwater.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Multidisciplinary Sciences
Tristan A. Caro, Jamie McFarlin, Sierra Jech, Noah Fierer, Sebastian Kopf
Summary: In this study, the growth rates of soil microorganisms were measured using hydrogen-stable isotope probing of lipids with 2H-enriched water. The results showed that microbial growth rates in soil are slow, with average generation times of 14 to 45 days. Furthermore, there is a decoupling of microbial abundance and growth in soil microbiomes. This study highlights the importance of measuring growth rates to complement standard analyses of soil microbial communities.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Agronomy
Andjin Siegenthaler, Andrew K. Skidmore, G. Arjen de Groot, Ivo Laros, Melody Rousseau, Yiwei Duan
Summary: This study reveals sphere-specific microbial communities in European temperate forests, characterized by low connectivity. It emphasizes the stronger influence of microbial spheres rather than host identity. Only a few taxa inhabit both the top-canopy and soil spheres, yet they make up the majority of the abundance in each sphere.
Article
Microbiology
Dariia Vyshenska, Pranav Sampara, Kanwar Singh, Andy Tomatsu, W. Berkeley Kauffman, Erin E. Nuccio, Steven J. Blazewicz, Jennifer Pett-Ridge, Katherine B. Louie, Neha Varghese, Matthew Kellom, Alicia Clum, Robert Riley, Simon Roux, Emiley A. Eloe-Fadrosh, Ryan M. Ziels, Rex R. Malmstrom
Summary: Answering the questions, who is eating what? and who is active? within complex microbial communities is essential for modeling, predicting, and modifying microbiomes for improved human and planetary health. Stable isotope probing can be used to track the incorporation of labeled compounds into cellular DNA during microbial growth to address these questions. However, traditional stable isotope methods face challenges in establishing links between an active microorganism's taxonomic identity and genome composition while providing quantitative estimates of the microorganism's isotope incorporation rate. This study presents an experimental and analytical workflow that improves the detection of metabolically active microorganisms and provides better quantitative estimates of genome-resolved isotope incorporation, thus refining ecosystem-scale models for carbon and nutrient fluxes within microbiomes.
Article
Agriculture, Dairy & Animal Science
Zhongyan Lu, Lingmeng Kong, Shenhao Ren, Jorg R. Aschenbach, Hong Shen
Summary: The rapid accumulation of organic acids, particularly lactate, is believed to be the main cause of ruminal acidosis (RA) in ruminants fed high-concentrate diets. However, the mechanisms behind this phenomenon are still not well understood. This study investigated the changes in ruminal microbiome and acidosis in goats fed diets with increasing concentrate portions. The results showed that an increase in dietary concentrate led to a sharp decrease in the abundance and expression of genes encoding lactate dehydrogenase, which is responsible for the conversion of pyruvate to lactate. These changes were attributed to specific bacterial groups and have important implications for the prevention of ruminal acidosis.
Article
Microbiology
Sara Campana, Kathrin Busch, Ute Hentschel, Gerard Muyzer, Jasper M. de Goeij
Summary: This study used DNA stable isotope probing and 16S rRNA amplicon sequencing to identify metabolically active bacterial taxa involved in dissolved organic matter (DOM) uptake in a sponge holobiont. The results revealed that specific bacterial taxa belonging to PAUC34f, Poribacteria, and Chloroflexi phyla are active consumers of DOM through heterotrophic carbon metabolism, while Nitrospirae may have mixotrophic capabilities. This research provides new insights into the interaction between diverse sponge-associated bacteria and DOM consumption in marine ecosystems.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Environmental Sciences
Dora Pavic, Dorotea Grbin, Amalija Blagajac, Josip Curko, Zeljka Fiket, Ana Bielen
Summary: This study aimed to correlate the physico-chemical parameters of water with the microbial community structure and the occurrence of putative bioindicator taxa. The results revealed positive correlations between certain microbial taxa and water parameters, such as ion content and nutrient content. Uranium was found to have the highest number of positive correlations with microbial taxa. These findings will contribute to the development of protocols for eDNA-based biological assessment of water quality.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Microbiology
Peng-Tao Pei, Lu Liu, Xiao-Li Jing, Xiao-Lu Liu, Lu-Yang Sun, Chen Gao, Xiao-Han Cui, Jing Wang, Zhong-Lian Ma, Shu-Yue Song, Zhi-Hua Sun, Chang-Yun Wang
Summary: The study found that the geographical factors have a stronger influence on the structure of coral microbiota than the host species. As the geographical distance increases, the deterministic process plays a more significant role in shaping the bacterial community composition.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Ecology
Maria Mooshammer, Katharina Kitzinger, Arno Schintlmeister, Soeren Ahmerkamp, Jeppe Lund Nielsen, Per Halkjaer Nielsen, Michael Wagner
Summary: The study introduces a SIP approach that significantly reduces cross-feeding in complex microbial communities by continuously exposing microbial cells to isotopically labeled substrate on a membrane filter. This approach removes metabolites and degradation products constantly, preventing the consumption of secondary substrates, thus allowing for the distinction between primary consumers and other members of microbial food webs.
Article
Environmental Sciences
Huanjun Zhang, Yin Lu, Yi Li, Lei Wang, Wenlong Zhang, Longfei Wang, Lihua Niu, Zhongjun Jia
Summary: This study identified bacteria involved in estrogen metabolism in sediments from different bays of Taihu Lake using DNA-SIP technology, revealing their distinct roles in the biodegradation process of E2. The results showed a significant and positive correlation between the increase in relative abundance of these bacteria and E2 mineralization rate.
ENVIRONMENTAL POLLUTION
(2021)
Article
Environmental Sciences
Weimin Sun, Xiaoxu Sun
Summary: This study found that methane can be produced through anaerobic degradation of toluene over a long-term enrichment experiment. The DNA-SIP technique identified bacteria in the phylum Proteobacteria as the dominant toluene-degrading bacteria under anaerobic conditions.
WATER AIR AND SOIL POLLUTION
(2021)
Article
Thermodynamics
Mengdi Cui, Baolong Wang, Falin Wei, Wenxing Shi
Summary: The study proposes a modified exergy analysis method to locate major imperfections and determine optimization directions for developing high-efficiency vapor compression systems, achieving significant improvements in coefficient of performance for air source heat pump water heaters and room air conditioners through improving energy efficiency of compressors and decreasing refrigerant exergy destruction.
APPLIED THERMAL ENGINEERING
(2022)
Article
Biochemical Research Methods
Fuhao Zhang, Bi Zhao, Wenbo Shi, Min Li, Lukasz Kurgan
Summary: This study presents the development of an innovative deep multi-task architecture, DeepDISOBind, which accurately predicts intrinsically disordered regions (IDRs) that interact with nucleic acids and proteins in protein sequences. The results show significant improvements in predictive accuracy compared to single-task designs and existing methods. Analysis of human proteome data demonstrates the ability of DeepDISOBind to accurately predict DNA- and RNA-binding proteins and protein hubs.
BRIEFINGS IN BIOINFORMATICS
(2022)
Article
Construction & Building Technology
Zixu Yang, Xian Dong, Hansong Xiao, Hongli Sun, Baolong Wang, Wenxing Shi, Xianting Li
Summary: The main factors affecting thermal comfort of convective terminals during the heating season have been extensively studied in recent years. This investigation aimed to analyze thermal comfort under typical settings and integrated control strategies. The study used a questionnaire, laboratory measurements, and artificial chamber simulations to examine the effects of draft, vertical temperature difference, and control strategies. The results showed that people were more tolerant of draft than vertical temperature differences and that perceived control significantly reduced discomfort. There were no significant differences between different control strategies, except for a long-term shutdown.
BUILDING AND ENVIRONMENT
(2022)
Article
Environmental Sciences
Zhanhua Zhou, Kim Yrjala, Junhui Chen, Chenliang Yu, Wenhui Shi, Hua Qin, Weiwu Yu, Wensheng Dai, Yuanyuan Hu, Jiasheng Wu
Summary: The combination of biochar and sheep manure significantly improved soil fertility and plant growth in a Torreya grandis plantation, demonstrating significant effects on plant quality and soil nutrients when compared to individual treatments with manure or rapeseed cake.
JOURNAL OF SOILS AND SEDIMENTS
(2022)
Article
Biochemistry & Molecular Biology
Wentao Shi, Lu Bian, Yiqing Wu, Zhe Wang, Yao Dai, Yanjun Zhou, Pengfei Meng, Qing Wang, Zhijian Zhang, Xijiang Zhao, Peng Zhao, Xiaojie Lu
Summary: In this study, fibrin-based biomaterials made of ZIF-8 and fibrin gel (Z-FG) were fabricated to enhance skull regeneration. The physical properties of Z-FG were investigated and its potential as an osteoinductive biomaterial was evaluated through assessments of bone-related proteins, calcium deposition, and alkaline phosphatase activity. The in vivo results showed increased bone formation in rat calvarial defects, suggesting that Z-FG may be a suitable biomaterial for skull tissue-engineering applications.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Materials Science, Multidisciplinary
Weiming Lv, Yiqun Wang, Wenhua Shi, Wei Cheng, Rong Huang, Ruifang Zhong, Zhongming Zeng, Yaming Fan, Baoshun Zhang
Summary: The study found that the micro-nano fabrication process significantly affects the temperature coefficient of resistance of Pt RTD, with both chemical polishing and annealing processes effectively improving TCR.
Article
Engineering, Environmental
Bijiang Geng, Shirui Zhang, Xue Yang, Wenyan Shi, Ping Li, Dengyu Pan, Longxiang Shen
Summary: This study developed a novel heterojunction nanosonosensitizer with enhanced production of singlet oxygen and hydroxyl radicals for sonodynamic therapy. The nanosonosensitizer effectively separated the electron-hole pairs generated by ultrasound through a direct Z-scheme charge transfer mechanism. The experiments demonstrated complete tumor eradication in osteosarcoma models using a single drug injection and single ultrasound irradiation.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Environmental Sciences
Bing Zhang, Heli Tang, Xiaolong Gu, Xiaohong Li, Bing Zhang, Yu Shen, Wenxin Shi
Summary: This study investigates the effects of Na+ and K+ on APAM-based membrane fouling and reveals the mechanisms behind the fouling. The research finds that an increase in the ionic strength of Na+ and K+ exacerbates the reduction of relative flux and the accumulation of fouling resistance, while also causing the fouling layer to become denser. Furthermore, K+ has a stronger effect on membrane fouling compared to Na+.
Article
Mathematics, Interdisciplinary Applications
Wanbo Lu, Wenhui Shi
Summary: This paper proposes a model averaging estimation method for multiplicative error model and constructs a corresponding weight choosing criterion. The KLMA estimator is proved to be asymptotically optimal and improves the forecast accuracy of out-of-sample.
Article
Immunology
Mingyue Cai, Wensou Huang, Jingjun Huang, Wenbo Shi, Yongjian Guo, Licong Liang, Jingwen Zhou, Liteng Lin, Bihui Cao, Ye Chen, Juan Zhou, Kangshun Zhu
Summary: TACE-L-P significantly improves survival in advanced HCC patients with acceptable safety, especially in those without main portal vein invasion but with extrahepatic metastasis or tumor number >3.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Wentao Shi, Zhijia Shen, Lu Bian, Yan Gao, Yiqing Wu, Feifei Zhao, Hong Tang, Xiaojie Lu
Summary: DOPA is a key component in the solidification and adhesion of mussel adhesives, and its deposition on various surfaces has been used to create biomimetic coatings. In this study, DOPA derivatives and AL were utilized to form an organic-inorganic hybrid coating that supports stem cell growth and osteogenic differentiation.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2022)
Article
Genetics & Heredity
Zimeng Chen, Li He, Song Weicai, Wenbo Shi, Qin Gong, Chao Shi
Summary: This study presents the first complete chloroplast genome sequence of Actinodaphne lecomtei, providing insights into its genome structure and phylogenetic relationship. The analysis reveals a close relationship between A. lecomtei and A. obovate.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Environmental Sciences
Yuan Guo, Bing Zhang, Siqi Feng, Dongqi Wang, Jiake Li, Wenxin Shi
Summary: This study systematically demonstrated the significance of Ca2+ ion for the start-up of AGS reactors and its influence on different properties of granules.
ENVIRONMENTAL RESEARCH
(2022)
Article
Chemistry, Applied
Liting Wan, Lin Li, Xia Zhang, Bing Li, Janette M. Harro, Mark E. Shirtliff
Summary: This study aimed to investigate the impacts of dietary lipid composition and content on the cecal and fecal microbiota in mice. The results showed that increasing the dietary palm stearin (PS) level promoted the growth of Firmicutes and resulted in higher lipid and bile acid content in feces. However, increasing palm olein (PO) intake did not affect the microbial structure. Only at a high-fat level, PS (compared to PO) induced the observed microbial shifts. These findings highlight the importance of both lipid quality and quantity on gut microbiota.
Article
Business, Finance
Wen Shi, Xiaogang Bi
Summary: This research examines the impact of Buddhism on mergers & acquisitions (M&As) performance using data from 4622 M&A transactions of Chinese public firms between 2004 and 2018. The findings provide ample evidence that acquirers located in regions with a higher intensity of Buddhism achieve better short-term and long-term performance after the merger. Furthermore, the study reveals that state ownership plays a crucial role in mitigating this effect, and the value creation is driven by higher deal completion rates and fewer violation cases for acquiring firms. Finally, the study addresses the issue of endogeneity, and the results remain unchanged.
ACCOUNTING AND FINANCE
(2023)