Article
Biochemistry & Molecular Biology
Benjamin Ober-Reynolds, Winston R. Becker, Karina Jouravleva, Samson M. Jolly, Phillip D. Zamore, William J. Greenleaf
Summary: Studies have shown that TtAgo uses biophysical adaptations similar to those of eukaryotic Argonautes for rapid association but requires more extensive complementarity to achieve high-affinity target binding. The cleavage rates of TtAgo vary widely based on the DNA guide, suggesting that only a subset of guides cleaves targets on physiologically relevant timescales.
Article
Environmental Sciences
Wending Li, Xuedan Xu, Qin Jiang, Pinpin Long, Yang Xiao, Yutong You, Chengyong Jia, Wei Wang, Yanshou Lei, Jianjian Xu, Yufei Wang, Min Zhang, Chong Liu, Qiang Zeng, Shuping Ruan, Xiaozheng Wang, Chaolong Wang, Yu Yuan, Huan Guo, Tangchun Wu
Summary: This study reveals the association between circulating metals and miRNA signatures in leukocytes at common exposure levels, and identifies the potential roles of these miRNAs in biological processes in the immune system.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Plant Sciences
Claudia Gutierrez-Garcia, Shiek S. S. J. Ahmed, Sathishkumar Ramalingam, Dhivya Selvaraj, Aashish Srivastava, Sujay Paul, Ashutosh Sharma
Summary: This study utilized high-throughput sequencing technology to analyze the miRNA profile of Murraya koenigii and identified miRNAs associated with secondary metabolite biosynthesis. The results showed that these miRNAs targeted key enzymes in the terpenoid backbone and flavonoid biosynthesis pathways. The sequencing results were validated by qPCR. This is the first report of the miRNA profile of Murraya koenigii, providing valuable information for studying the involvement of miRNAs in secondary metabolism.
Article
Multidisciplinary Sciences
Suzie K. Hight, Trevor N. Clark, Kenji L. Kurita, Elizabeth A. McMillan, Walter Bray, Anam F. Shaikh, Aswad Khadilkar, F. P. Jake Haeckl, Fausto Carnevale-Neto, Scott La, Akshar Lohith, Rachel M. Vaden, Jeon Lee, Shuguang Wei, R. Scott Lokey, Michael A. White, Roger G. Linington, John B. MacMillan
Summary: Determining the mechanism of action of natural products is a significant challenge. This study presents a comprehensive platform that integrates multiple data sources and metabolomics analysis to improve the prediction of mechanism of action and facilitate the identification of bioactive metabolites.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Saquib Waheed, Fan Liang, Mengyuan Zhang, Dayi He, Lihui Zeng
Summary: This study compared differentially expressed miRNAs (DEmiRNAs) and their target genes between a continuous flowering cultivar and a unique cultivar that only blossoms once. The study identified several miRNAs and target genes associated with the continuous flowering trait, as well as critical regulatory pathways and flowering-related genes. Furthermore, the study discovered miRNAs that may play vital roles in flower regulation in longan.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Arkadiusz Kajdasz, Weronika Majer, Katarzyna Kluzek, Jacek Sobkowiak, Tomasz Milecki, Natalia Derebecka, Zbigniew Kwias, Hans A. R. Bluyssen, Joanna Wesoly
Summary: This study aimed to investigate changes in miRNA expression in tissue samples of renal cancer patients and identified a group of deregulated miRNAs in renal cancer and its subtypes through meta-analysis. The results suggested that changes in miRNA expression could potentially serve as a tool aiding ccRCC subclass discrimination.
Article
Biochemical Research Methods
Ming Wang, Mui Hoon Nai, Ruby Yun-Ju Huang, Hwa Liang Leo, Chwee Teck Lim, Chia-Hung Chen
Summary: A novel hydrogel drop-screen device was developed for rapid measurement of single-cell morphologies and secretions, revealing functional heterogeneities associated with cellular biological functions. The level of cell heterogeneity increased with increasing substrate stuffiness, and a database related to breast cells was constructed to correspond to the progress of tumor metastasis based on morphologies and secreted bioindicators.
Review
Mathematical & Computational Biology
Mengya Chai, Xueyang Xiong, Huimin Wang, Lida Xu
Summary: This study discusses the bias issue in constructing miRNA sequencing libraries and suggests strategies to minimize bias and generate high-quality data. Improving adapter design, optimizing ligation conditions, using high-quality RNA samples, removing adapter dimers, optimizing PCR, and product purification are essential for obtaining high-quality sequencing data. Several protocols utilizing combinatorial optimization and commercially available low-input miRNA library preparation kits are also described. The study provides the basis for unbiased high-throughput quantification of miRNAs and can guide optimal miRNA profiling design for clinical diagnosis and treatment.
QUANTITATIVE BIOLOGY
(2022)
Article
Engineering, Environmental
Feika Bian, Hanxu Chen, Lingyu Sun, Ning Li, Yuanjin Zhao
Summary: This article presents a novel AIEgens-integrated structural color barcode for binary screening of miRNAs, which can provide stable structural colors and variable fluorescence signals. The barcode enables the multiplexed screening of miRNAs in a microfluidics device, showing great potential in biomedical fields.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Kai Xie, Zixun Wang, Lin Qi, Xi Zhao, Yuan Wang, Jin Qu, Ping Xu, Linfeng Huang, Wenjun Zhang, Yang Yang, Xin Wang, Peng Shi
Summary: The described platform allows for multiplexed in situ miRNA profiling in acute tissue slices, providing relative spatial dynamics of cellular miRNAs in associated cell populations. The technique isolates targeted miRNAs from the cytosol of a large population of cells to achieve quasi-single-cell analysis for the tissue sample.
Article
Clinical Neurology
Amber Yaqub, Michelle M. J. Mens, Jaco M. Klap, Gerrit Jan Weverling, Paul Klatser, Just P. J. Brakenhoff, Gennady Roshchupkin, Mohammad Kamran Ikram, Mohsen Ghanbari, Mohammad Arfan Ikram
Summary: We conducted a study to identify circulating miRNAs in plasma associated with cognition and incident dementia. Four miRNAs were found to be commonly associated with both cognition and dementia, and their target genes were expressed in the brain, suggesting their potential involvement in dementia pathways.
ALZHEIMERS & DEMENTIA
(2023)
Article
Microbiology
Zhichao Zhou, Patricia Q. Tran, Adam M. Breister, Yang Liu, Kristopher Kieft, Elise S. Cowley, Ulas Karaoz, Karthik Anantharaman
Summary: Advances in microbiome science have been driven by the ability to study and infer microbial ecology from genomes. METABOLIC is a scalable software that allows for the study of microbial ecology and biogeochemistry using genomes at the resolution of individual organisms and/or microbial communities. It provides annotation of microbial genomes, metabolic pathway analyses, and determination of microbial contributions to biogeochemical transformations and cycles. METABOLIC is a versatile tool that can be used with various metagenomic datasets, from diverse environments such as marine subsurface, terrestrial subsurface, and human gut.
Review
Oncology
Po-Shuan Huang, Chia-Jung Liao, Ya-Hui Huang, Chau-Ting Yeh, Cheng-Yi Chen, Hui-Chi Tang, Cheng-Chih Chang, Kwang-Huei Lin
Summary: Liver cancer, with its high mortality rate globally, lacks early diagnostic tools and effective treatment methods. MicroRNAs play important roles in liver cancer treatment, but there are challenges in improving diagnostic technologies and addressing the side effects of microRNA-based drugs.
Article
Medicine, Research & Experimental
Saira R. Ali, Karen J. Humphreys, Kaylene. J. Simpson, Ross A. McKinnon, Robyn Meech, Michael Z. Michael
Summary: Butyrate, a gut fermentation product, shows anti-cancer properties in the human proximal colon by inhibiting proliferation and inducing apoptosis in colorectal cancer cells. A study identified several miRNAs such as miR-125b, miR-181a, miR-593, and miR-1227 that can enhance the anti-cancer effects of butyrate, potentially affecting critical cancer-related growth pathways.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Chemistry, Multidisciplinary
Adrianna N. Masterson, Nayela N. Chowdhury, Yue Fang, Michele T. Yip-Schneider, Sumon Hati, Prashant Gupta, Sha Cao, Huangbing Wu, C. Max Schmidt, Melissa L. Fishel, Rajesh Sardar
Summary: In this study, an advanced nanoplasmonic sensor was developed for the sensitive and amplification-free detection of microRNAs in unprocessed plasma microsamples. The sensor utilized specially designed -ssDNA receptors attached to gold triangular nanoprisms to achieve highly specific detection. The sensor showed an attomolar limit of detection in human plasma and enabled high-throughput assay in a multiwell plate format. The performance of the sensor was validated using clinical samples, demonstrating its ability to differentiate pancreatic ductal adenocarcinoma (PDAC) from other conditions and determine PDAC stages. Furthermore, the sensor showed potential in monitoring microRNA changes in PDAC patients before and after surgery.
Article
Immunology
Yan-Jiang Wang, Chang-Yue Gao, Miao Yang, Xiao-Hong Liu, Yin Sun, Anthony Pollard, Xiao-Yan Dong, Xiao-Bing Wu, Jin-Hua Zhong, Hua-Dong Zhou, Xin-Fu Zhou
BRAIN BEHAVIOR AND IMMUNITY
(2010)
Article
Oncology
Jin Hou, Li Lin, Weiping Zhou, Zhengxin Wang, Guoshan Ding, Qiongzhu Dong, Lunxiu Qin, Xiaobing Wu, Yuanyuan Zheng, Yun Yang, Wei Tian, Qian Zhang, Chunmei Wang, Qinghua Zhang, Shi-Mei Zhuang, Limin Zheng, Anmin Liang, Wenzhao Tao, Xuetao Cao
Article
Immunology
Gencheng Han, Renxi Wang, Guojiang Chen, Ranan Wang, Ruonan Xu, Jiannan Feng, Ming Yu, Xiaobing Wu, Jiahua Qian, Beifen Shen, Yan Li
IMMUNOLOGY LETTERS
(2008)
Article
Virology
Changchun Cao, Xiaoyan Dong, Xiaobing Wu, Boyun Wen, Gang Ji, Lingpeng Cheng, Hongrong Liu
JOURNAL OF VIROLOGY
(2012)
Article
Geriatrics & Gerontology
Yan-Jiang Wang, Anthony Pollard, Jin-Hua Zhong, Xiao-Yan Dong, Xiao-Bing Wu, Hua-Dong Zhou, Xin-Fu Zhou
NEUROBIOLOGY OF AGING
(2009)
Article
Multidisciplinary Sciences
Jia Wu, Fengwei Zhang, Maorong Wang, Chunqiong Xu, Jingdong Song, Jianfang Zhou, Xiaojing Lin, Yonghui Zhang, Xiaobing Wu, Wenjie Tan, Jian Lu, Honglan Zhao, Jimin Gao, Ping Zhao, Jianxin Lu, Yue Wang
Article
Multidisciplinary Sciences
Xiaoyan Dong, Wenhong Tian, Gang Wang, Zheyue Dong, Wei Shen, Gang Zheng, Xiaobing Wu, Jinglun Xue, Yue Wang, Jinzhong Chen
Article
Biology
Wang Gang, Dong XiaoYan, Hu JianYang, Tian WenHong, Yuchi Jie, Wang Yue, Wu XiaoBing
SCIENCE CHINA-LIFE SCIENCES
(2011)
Article
Multidisciplinary Sciences
Qingzhang Zhou, Wenhong Tian, Chunguo Liu, Zhonghui Lian, Xiaoyan Dong, Xiaobing Wu
SCIENTIFIC REPORTS
(2017)
Article
Oncology
Gang Wang, Xiaoyan Dong, Wenhong Tian, Yue Lu, Jianyan Hu, Yunfan Liu, Jie Yuchi, Xiaobing Wu
CHINESE JOURNAL OF CANCER RESEARCH
(2013)
Article
Ophthalmology
Shenghai Zhang, Jihong Wu, Xiaobing Wu, Ping Xu, Yuhua Tian, Miaoying Yi, Xinjian Liu, Xiaoyan Dong, Frank Wolf, Chuanyuan Li, Qian Huang
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2012)
Article
Gastroenterology & Hepatology
TY Tsui, CK Lau, J Ma, XB Wu, YQ Wang, S Farkas, R Xu, HJ Schlitt, ST Fan
Article
Immunology
GC Han, Y Li, JN Wang, RX Wang, GJ Chen, L Song, RN Xu, M Yu, XB Wu, JH Qian, BF Shen
JOURNAL OF IMMUNOLOGY
(2005)
Article
Medicine, General & Internal
K Gao, YJ Wu, XB Wu, CM Rao, JZ Wang
CHINESE MEDICAL JOURNAL
(2004)