Article
Chemistry, Multidisciplinary
Saki Matsumoto, Hisae Tateishi-Karimata, Naoki Sugimoto
Summary: Understanding the regulatory mechanisms of DNA methylation in response to environmental changes is crucial and urgent. This study reveals that G-quadruplex (G4) stability and topology play a role in DNA methylation regulation. The higher the stability of G4, the lower the methylation efficiency. Furthermore, the topology of G4 can also control the degree of methylation suppression. These findings suggest the possibility of regulating methylation by modulating G4 stability and topology.
CHEMICAL COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Anqi Yang, Saina Yan, Yanfei Yin, Chujie Chen, Xiangwei Tang, Maoliang Ran, Bin Chen
Summary: In this study, the regulatory roles of non-CpG methylation in testicular development were explored by analyzing the whole-genome bisulfite sequencing and RNA sequencing data of porcine testicular tissues. It was found that non-CpG methylation regulates the expression of key genes involved in testes development. The importance of FZD7 in the proliferation and apoptosis of Sertoli cells was also demonstrated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Yeon-Su Lee, Yong Sun Lee
Summary: nc886 is a medium-sized non-coding RNA transcribed by RNA polymerase III (Pol III) and has diverse roles in tumorigenesis, innate immunity, and other cellular processes. The expression of nc886 is controlled by multiple mechanisms, including promoter CpG DNA methylation and transcription factor activity. Additionally, its RNA instability contributes to its highly variable expression levels. This comprehensive review discusses nc886's variable expression in physiological and pathological conditions and examines the regulatory factors that determine its expression levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Mary L. Clarke, Roza B. Lemma, David S. Walton, Giacomo Volpe, Boris Noyvert, Odd S. Gabrielsen, Jon Frampton
Summary: MYB is crucial for gene regulation in hematopoietic hierarchy and maintaining normal hematopoietic stem cells (HSC). Genetic dysregulation of MYB is involved in leukemias, while inherited noncoding variants of MYB contribute to various hematological conditions. However, the mechanism linking MYB levels to disease predisposition, especially age-related disease initiation, remains unknown. Our mouse model of Myb insufficiency shows development of MPN, myelodysplasia, and leukemia in later life, mimicking the age profile of human diseases. In young Myb-insufficient mice, we observe altered proteasomal activity and elevated proliferation indicators, suggesting an imbalance in proteostasis contributing to disease initiation.
Article
Cell Biology
Mengjia Li, Penglei Jiang, Kai Cheng, Zehui Zhang, Shuyu Lan, Xiaoxia Li, Lirong Zhao, Yucheng Wang, Xiang Wang, Jing Chen, Tao Ji, Bingshe Han, Junfang Zhang
Summary: The enhancer elements at -34k are crucial for MYB expression, with transcription factor binding and epigenetic modifications playing key roles in this process.
CELL DEATH & DISEASE
(2021)
Article
Genetics & Heredity
Katerina Athanasopoulou, Vasiliki Chondrou, Panagiotis Xiropotamos, Georgios Psarias, Yiannis Vasilopoulos, Georgios K. Georgakilas, Argyro Sgourou
Summary: Non-coding RNA species, particularly long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), have been found to play a role in human erythropoiesis. A transcription factor called lymphoma/leukemia-related (LRF) has been identified as an important regulator of chromatin remodeling and hemoglobin transversion during erythropoiesis. This study investigates the potential repressor role of LRF in lncRNAs and miRNAs during erythropoiesis.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Genetics & Heredity
Vikramjit K. Zhawar, Raj P. Kandpal, Raghbir S. Athwal
Summary: This study explores the cell-type-specific silencing of GluR6 gene promoters through epigenetic mechanisms. The neuronal promoter is methylated in non-neuronal cells, while the treatment can upregulate the transcription of the GluR6 gene. Bisulfite sequencing reveals differential CpG site methylation in non-neuronal and neuronal promoters.
Article
Multidisciplinary Sciences
Paula Metselaar, Lucero Mendoza-Maldonado, Andrew Yung Fong Li Yim, Ilias Abarkan, Peter Henneman, Anje A. te Velde, Alexander Schoenhuth, Jos A. Bosch, Aletta D. Kraneveld, Alejandro Lopez-Rincon
Summary: The study combined mRNA expression and DNA methylation data to identify a gene signature that distinguishes ME/CFS patients from healthy controls. The results suggest that these genes are related to the dysregulated immune system in ME/CFS patients.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Viera Horvathova Kajabova, Andrea Soltysova, Lucia Demkova, Paulina Plesnikova, Darina Lyskova, Alena Furdova, Pavel Babal, Bozena Smolkova
Summary: Our study found that KIT positivity was associated with a more than 17-fold increase in the risk of poor prognosis in uveal melanoma, and a strong negative correlation was identified between mRNA expression and DNA methylation values for 12 of 20 analyzed positions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Teresa Improda, Valentina Morgera, Maria Vitale, Lorenzo Chiariotti, Fabiana Passaro, Antonia Feola, Antonio Porcellini, Mariella Cuomo, Antonio Pezone
Summary: Cell identity is determined by chromatin structure and gene expression profiles, which rely on chromatin accessibility and DNA methylation. These epigenetic modifications are crucial for mammalian development and cellular identity. Dynamic DNA methylation and demethylation occur during cell fate commitment and terminal differentiation. By analyzing the methylation configurations of gene promoters during murine brain differentiation, we found significant methyl-CpG profiles associated with gene silencing or activation. Interestingly, these methylation patterns mark different brain areas and cell types during differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
In-Chi Young, Bogang Wu, Jaclyn Andricovich, Sung-Ting Chuang, Rong Li, Alexandros Tzatsos, Ray-Chang Wu, Mei-Yi Wu
Summary: ARID4B interferes with KITLG/KIT signaling, promoting HSC differentiation instead of self-renewal. ARID4B-deficient HSCs self-express KITLG and overexpress KIT, leading to differentiation. Inhibition of Src family kinases rescues the HSC differentiation defect caused by ARID4B loss.
Article
Cell & Tissue Engineering
Youmna S. Kfoury, Fei Ji, Michael Mazzola, David B. Sykes, Allison K. Scherer, Anthony Anselmo, Yasutoshi Akiyama, Francois Mercier, Nicolas Severe, Konstantinos D. Kokkaliaris, Ting Zhao, Thomas Brouse, Borja Saez, Jefferson Seidl, Ani Papazian, Pavel Ivanov, Michael K. Mansour, Ruslan Sadreyev, David T. Scadden
Summary: The study demonstrates that osteoblastic cells in the bone marrow niche secrete extracellular vesicles containing tiRNAs that can be transferred to hematopoietic progenitor cells, impacting protein translation, cell proliferation, and myeloid differentiation. This transfer of EVs improves hematopoietic recovery from genotoxic injury and survival from fungal sepsis, suggesting a stress-modulated signaling axis in the bone marrow microenvironment distinct from conventional cytokine-driven responses.
Article
Biochemistry & Molecular Biology
Suman Abhishek, Naveen Kumar Nakarakanti, Waghela Deeksha, Eerappa Rajakumara
Summary: UHRF1 SRA can recognize non-mCpG DNA, with contrasting mechanisms for hemi-mCpG and fully-mCpHpG DNA recognition. While the binding affinity for fully-mCpG is weaker, UHRF1 SRA exhibits higher affinity for fully-mCpHpG.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Plant Sciences
Zeyu Chen, Junwei Yuan, Yi Yao, Jiahao Cao, Wenjie Yang, Yu Long, Juanxu Liu, Weiyuan Yang
Summary: In this study, the authors identified the petunia anthocyanin acyltransferase gene, PhAAT1, which is located in the cytoplasm. Silencing of PhAAT1 resulted in changes in flower color and reduced the stability of anthocyanins. Metabolomics analysis showed a decrease in p-coumaroylated and caffeoylated anthocyanins upon PhAAT1 silencing. Additionally, PhAAT1 was found to be positively regulated by the MYB transcription factor, PhAN2, which directly interacts with the promoter of PhAAT1.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Biochemistry & Molecular Biology
Tiziana Raia, Federica Armeli, Rosaria A. Cavallaro, Giampiero Ferraguti, Rita Businaro, Marco Lucarelli, Andrea Fuso
Summary: DNA methylation, the main epigenetic modification, influences neurodegeneration. In this study, the researchers investigated the effects of S-adenosylmethionine (SAM) supplementation during the perinatal period on the development of Alzheimer's disease (AD) symptoms in TgCRND8 mice. They found that both post-weaning and perinatal SAM supplementation effectively reduced PSEN1 expression and amyloid deposition in adult mice. These findings emphasize the role of early-life epigenetic memory and methyl donor availability in promoting healthy aging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Xianying A. Cui, Hui Zhang, Lena Ilan, Ai Xin Liu, Iryna Kharchuk, Alexander F. Palazzo
JOURNAL OF CELL SCIENCE
(2015)
Article
Biochemistry & Molecular Biology
D. Kolodkin-Gal, Y. Edden, Z. Hartshtark, L. Ilan, A. Khalaileh, A. J. Pikarsky, E. Pikarsky, S. D. Rabkin, A. Panet, G. Zamir
Article
Biochemistry & Molecular Biology
Raymond Kaempfer, Lise Sarah Namer, Farhat Osman, Lena Ilan
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Shulamit Sebban, Marganit Farago, Dan Gashai, Lena Ilan, Eli Pikarsky, Ittai Ben-Porath, Shulamit Katzav
Article
Oncology
Shulamit Sebban, Marganit Farago, Shiran Rabinovich, Galit Lazer, Yulia Idelchuck, Lena Ilan, Eli Pikarsky, Shulamit Katzav
Article
Cell Biology
Lena Ilan, Farhat Osman, Lise Sarah Namer, Einav Eliahu, Smadar Cohen-Chalamish, Yitzhak Ben-Asouli, Yona Banai, Raymond Kaempfer
Article
Genetics & Heredity
MO Mosig, E Lipkin, G Khutoreskaya, E Tchourzyna, M Soller, A Friedmann