Article
Zoology
Abu Salman Talukder, Nusrat Jahan Punom, Md Mostavi Enan Eshik, Mst Khadiza Begum, H. M. Rakibul Islam, Zakir Hossain, Mohammad Shamsur Rahman
Summary: White spot disease has caused significant economic losses in the shrimp farming industry in Bangladesh over the past two decades. The study conducted in the southwest region of Bangladesh in 2018 identified nine significant risk factors for WSD, confirmed WSSV infection, and found a high prevalence of WSSV in the Khulna region with strains identical to those in India. The relationships between risk factors, disease prevalence and severity, and WSSV strains could greatly impact WSD management.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2021)
Article
Fisheries
Lu Zhang, Yan Wang, Jingjie Hu, Zhenmin Bao, Mengqiang Wang
Summary: White spot syndrome virus (WSSV), a major threat to shrimp farming, lacks effective therapeutics. This study developed an enzymatic recombinase amplification (ERA) assay for rapid and sensitive detection of WSSV. The assay showed high specificity, short detection time, and accuracy in detecting WSSV in shrimp farms, providing an alternative method for controlling WSSV infection.
Article
Multidisciplinary Sciences
Pasunee Laohawutthichai, Thapanan Jatuyosporn, Premruethai Supungul, Anchalee Tassanakajon, Kuakarun Krusong
Summary: This research investigates the effects of PmDOME and PmSTAT silencing on immune-related gene expressions in shrimp hemocytes during WSSV infection. It found that the silencing of PmDOME and PmSTAT significantly impacted the expression levels of multiple immune-related genes and reduced WSSV replication and mortality. The study suggests that suppression of the JAK/STAT signaling pathway may delay WSSV-related mortality by activating immune responses.
SCIENTIFIC REPORTS
(2023)
Article
Fisheries
Annies Joseph, Rosamma Philip
Summary: This study aimed to determine the influence of copper on the physiological and immunological responses of Penaeus monodon to white spot syndrome virus (WSSV) infection. The results showed that exposure to 0.1 mg/l copper concentration enhanced the immune response and resistance to WSSV infection in the shrimp, resulting in higher survival. However, exposure to 0.2 and 0.3 mg/l copper concentrations increased susceptibility to WSSV infection, leading to a decrease in biochemical/immune variables.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Qi Wang, Yinglei Xu, Chongyang Xiao, Fei Zhu
Summary: In this study, the recombinant protein VP28 (VP26 or VP24) was used for immune protection experiment. Crayfish immunized with VP28 showed a higher survival rate compared to those immunized with VP26 or VP24 after WSSV challenge. VP28 immunization inhibited WSSV replication in crayfish, increasing the survival rate to 66.67% after infection. VP28 treatment enhanced the expression of immune genes, total hemocyte counts, and enzyme activities in crayfish. It also reduced hemocyte apoptosis, both before and after WSSV infection.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Vernita Priya, Sudhakaran Raja
Summary: Shrimp aquaculture is booming because of high demand in developed countries, but the white spot syndrome virus (WSSV) is causing massive mortality in shrimp population. Nanotechnology provides advantages in targeted drug delivery, altering charge of nanoparticles, and minimizing adverse effects. This review discusses antiviral activity of CS/TPP-coated nanoparticles encapsulating WSSV VP28, stimulation of innate immunity using polyamine-coated carbon quantum dots, and investigation of silver nanoparticles in inhibiting viral activity for effective cure of white spot syndrome.
AQUACULTURE INTERNATIONAL
(2023)
Article
Virology
Han Lei, Shuangqin Li, Xin Lu, Yi Ren
Summary: By utilizing a Saccharomyces cerevisiae surface display system, a potential vaccine candidate against White spot syndrome virus (WSSV) was developed and shown to induce significant immune-related enzyme activities in shrimp when orally administered. The oral vaccine conferred 100% protection and reduced viral load in shrimp, highlighting its potential as an efficient control strategy against WSSV infection in shrimp aquaculture.
Article
Multidisciplinary Sciences
Thapanan Jatuyosporn, Pasunee Laohawutthichai, Juan Pablo Ochoa Romo, Luigui Gallardo-Becerra, Filiberto Sanchez Lopez, Anchalee Tassanakajon, Adrian Ochoa-Leyva, Kuakarun Krusong
Summary: The gut microbiome is important for the immune system of both invertebrates and vertebrates. Pre and pro-biotics can enhance the shrimp immune system by increasing the activity of certain enzymes. Changes in the host immune system during viral infection can affect the gut microbiome and potentially lead to other infections.
SCIENTIFIC REPORTS
(2023)
Article
Fisheries
Chao Peng, Chao Zhao, Peng-Fei Wang, Lu-Lu Yan, Si-Gang Fan, Li-Hua Qiu
Summary: The research identified a novel TRIM32 homolog, PmTRIM32, in Penaeus monodon, which was significantly upregulated by rapamycin stimulation and WSSV infection. PmTRIM32 was found to restrict WSSV replication, positively regulate autophagy and NF-kappa B pathway, and interact with WSSV envelope protein for ubiquitination.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Microbiology
Mehedi Mahmudul Hasan, M. Nazmul Hoque, Firoz Ahmed, Md. Inja-Mamun Haque, Munawar Sultana, M. Anwar Hossain
Summary: This study investigated the occurrence and virulence of White Spot Syndrome Virus (WSSV) in different farming areas of Bangladesh. The prevalence of WSSV in shrimps and crabs varied significantly between study areas and study periods. The study also found that a single amino acid substitution in the VP28 gene could affect the virulence properties of WSSV, including mortality rate and viral load in artificially infected shrimps.
Article
Fisheries
Md. Lifat Rahi, Wasim Sabbir, Krishna R. Salin, Dania Aziz, David A. Hurwood
Summary: The study showed that the pathogens VP and WSSV significantly reduced hemocyte counts and increased glucose and serotonin levels in the hemolymph of black tiger shrimp. These pathogenic treatments altered the expression pattern of candidate genes and affected the physiological and biochemical parameters, resulting in negative impacts on growth and mortality of the experimental shrimps.
Article
Virology
Min-Jeong Kim, Su-Hyun Kim, Jong-Oh Kim, Taek-Kyun Lee, In-Kwon Jang, Tae-Jin Choi
Summary: VP28 protein expressed in Chlorella vulgaris can serve as an oral shrimp vaccine against white spot syndrome virus (WSSV), significantly reducing the mortality rate and upregulating the expression of shrimp defense genes.
Article
Fisheries
Ancy Thomas, Naduvilamuriparampu Saidumuhammed Sudheer, Viswanath Kiron, Bright Singh Issac Singh, Rangarajan Badri Narayanan
Summary: This paper investigates the effectiveness of the major antigenic proteins VP24 and VP28 from white spot syndrome virus (WSSV) in inducing an immune response in shrimp. The recombinant proteins were mass-produced and orally administered to shrimp, resulting in the upregulation of several biodefense genes. The study reveals the antigenic properties of VP24 and VP28 and their ability to elicit an immune response after oral administration.
AQUACULTURE RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
B. S. Anoop, Jayesh Puthumana, Vrinda Sukumaran, Cijo George Vazhappilly, Salini Kombiyil, Rosamma Philip, Isaac Sarojini Bright Singh
Summary: The development of shrimp cell lines has been attempted for over 30 years, with one of the main challenges being replicative senescence and inability to maintain telomere length in vitro. By inducing oncogenic transformation through a recombinant baculovirus containing H-ras downstream of the shrimp viral promoter IHHNV-P2, a fibroblast-like primary cell culture with confirmed H-ras and GFP expression was successfully developed. Transduction efficiency during early dividing stage of cells can be enhanced by adding 5 mM sodium butyrate to the culture medium.
MARINE BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Mostafa Rakhshaninejad, Liping Zheng, Hans Nauwynck
Summary: This study found that Penaeus vannamei shrimp infected with White spot syndrome virus (WSSV) use behavioral fever to increase their body temperature, inhibit virus replication, and prevent infection and mortality.
SCIENTIFIC REPORTS
(2023)