Article
Multidisciplinary Sciences
Jennifer Barrila, Jiseon Yang, Aurelie Crabbe, Shameema F. Sarker, Yulong Liu, C. Mark Ott, Mayra A. Nelman-Gonzalez, Simon J. Clemett, Seth D. Nydam, Rebecca J. Forsyth, Richard R. Davis, Brian E. Crucian, Heather Quiriarte, Kenneth L. Roland, Karen Brenneman, Clarence Sams, Christine Loscher, Cheryl A. Nickerson
Review
Immunology
Brian E. Crucian, Alexander Chouker, Richard J. Simpson, Satish Mehta, Gailen Marshall, Scott M. Smith, Sara R. Zwart, Martina Heer, Sergey Ponomarev, Alexandra Whitmire, Jean P. Frippiat, G. Douglas, H. Lorenzi, Judith-Irina Buchheim, George Makedonas, Geoffrey S. Ginsburg, C. Mark Ott, Duane L. Pierson, Stephanie S. Krieger, Natalie Baecker, Clarence Sams
FRONTIERS IN IMMUNOLOGY
(2018)
Review
Immunology
Jennifer Barrila, Aurelie Crabbe, Jiseon Yang, Karla Franco, Seth D. Nydam, Rebecca J. Forsyth, Richard R. Davis, Sandhya Gangaraju, C. Mark Ott, Carolyn B. Coyne, Mina J. Bissell, Cheryl A. Nickerson
INFECTION AND IMMUNITY
(2018)
Article
Microbiology
Madhan R. Tirumalai, Fathi Karouia, Quyen Tran, Victor G. Stepanov, Rebekah J. Bruce, C. Mark Ott, Duane L. Pierson, George E. Fox
Article
Multidisciplinary Sciences
Alexander A. Voorhies, C. Mark Ott, Satish Mehta, Duane L. Pierson, Brian E. Crucian, Alan Feiveson, Cherie M. Oubre, Manolito Torralba, Kelvin Moncera, Yun Zhang, Eduardo Zurek, Hernan A. Lorenzi
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Jennifer Barrila, Shameema F. Sarker, Nicole Hansmeier, Shanshan Yang, Kristina Buss, Natalia Briones, Jin Park, Richard R. Davis, Rebecca J. Forsyth, C. Mark Ott, Kevin Sato, Cristine Kosnik, Anthony Yang, Cheryl Shimoda, Nicole Rayl, Diana Ly, Aaron Landenberger, Stephanie D. Wilson, Naoko Yamazaki, Jason Steel, Camila Montano, Rolf U. Halden, Tom Cannon, Sarah L. Castro-Wallace, Cheryl A. Nickerson
Summary: This study investigated the effects of spaceflight on the physiology of human cells and microbial pathogens relevant to infectious disease, with a focus on the host-pathogen interactions. The results from the first in-flight infection and dual RNA-seq analysis using human cells provide valuable insights into the impact of space environment on cellular and molecular responses.
Article
Biotechnology & Applied Microbiology
Jiseon Yang, Jennifer Barrila, C. Mark Ott, Olivia King, Rebekah Bruce, Robert J. C. McLean, Cheryl A. Nickerson
Summary: This study utilized microbial isolates from the ISS potable water system to profile five phenotypes and revealed a temporal dependence on interactive behaviors, suggesting possible microbial adaptation over time within the ecosystem. The findings have implications for microbial risk assessments of water systems in built environments in space and on Earth.
NPJ BIOFILMS AND MICROBIOMES
(2021)
Article
Biology
Christina L. M. Khodadad, Cherie M. Oubre, Victoria A. Castro, Stephanie M. Flint, Monsi C. Roman, Charlie Mark Ott, Cory J. Spern, Mary E. Hummerick, Gretchen J. Maldonado Vazquez, Michele N. Birmele, Quinn Whitlock, Matt Scullion, Christina M. Flowers, Raymond M. Wheeler, Orlando Melendez
Summary: The research explored the environmental monitoring systems and technologies needed in closed space environments, successfully testing an instrument for microbial monitoring. Tests conducted on the space station showed that this technology can effectively detect target microorganisms, enhancing crew safety.
Editorial Material
Microbiology
Cheryl A. Nickerson, Audrie A. Medina-Colorado, Jennifer Barrila, George Poste, C. Mark Ott
Summary: Microbiological research has made significant discoveries about how life responds to non-terrestrial environments, such as the International Space Station. As human space exploration transitions to longer deep-space missions, microorganisms will continue to play a critical role in astronaut health, habitat sustainability, and mission success.
NATURE MICROBIOLOGY
(2022)
Article
Immunology
Jennifer Barrila, Jiseon Yang, Karla P. Franco P. Melendez, Shanshan Yang, Kristina Buss, Trenton J. Davis, Bruce J. Aronow, Heather D. Bean, Richard R. Davis, Rebecca J. Forsyth, C. Mark Ott, Sandhya Gangaraju, Bianca Y. Kang, Brian Hanratty, Seth D. Nydam, Eric A. Nauman, Wei Kong, Jason Steel, Cheryl A. Nickerson
Summary: This study investigates the impact of Low Shear Modeled Microgravity (LSMMG) culture on the colonization of Salmonella Typhimurium and human intestinal diseases. The results show that LSMMG culture enhances the colonization ability of Salmonella and that the colonization ability of the Delta hfq mutant strain is higher under LSMMG conditions compared to conventional shaking cultures. In addition, infection by Salmonella induces the expression of genes related to inflammation, tissue remodeling, and wound healing in host cells. This study expands our understanding of how physical forces can affect the early stages of human enteric salmonellosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Karla Franco Melendez, Keith Crenshaw, Jennifer Barrila, Jiseon Yang, Sandhya Gangaraju, Richard R. Davis, Rebecca J. Forsyth, C. Mark Ott, Rebin Kader, Roy Curtiss, Kenneth Roland, Cheryl A. Nickerson
Summary: The discovery that biomechanical forces regulate microbial virulence has shed light on the importance of physiological low fluid shear (LFS) forces in altering gene expression, stress responses, and virulence of Salmonella enterica serovar Typhimurium. This study investigated the role of RpoS in regulating the responses of Salmonella to physiological LFS, and found that while RpoS was important for handling oxidative and thermal stresses, it was dispensable for acid and bile salts stresses, as well as for adherence and survival within intestinal epithelial cells. These findings enhance our understanding of how physiological fluid shear forces modulate the physiology of S. Typhimurium and provide insights into its responses in the infected host.