Article
Physics, Fluids & Plasmas
Zicong Zhou
Summary: The study finds that confining a helical filament on a cylinder can create a bistable state. The bistable helix exhibits different phase diagrams and compositions in different regimes. Monte Carlo simulation further suggests the presence of bistable nonhelices in the third regime.
Article
Microbiology
Brody Barton, Addison Grinnell, Randy M. Morgenstein
Summary: Deletion of malate dehydrogenase from the tricarboxylic acid (TCA) cycle increases bacterial tolerance to the MreB depolymerizing drug A22, leading to changes in cell wall synthesis and an increase in cell wall precursors.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Microbiology
Celine Betti, Pietro Camozzi, Viola Gennaro, Mario G. Bianchetti, Martin Scoglio, Giacomo D. Simonetti, Gregorio P. Milani, Sebastiano A. G. Lava, Alessandra Ferrarini
Summary: This study documented cases of leukocytoclastic small-vessel vasculitis in children following symptomatic diseases caused by Mycoplasma pneumoniae infection. Patients with systemic vasculitis and skin-limited vasculitis did not significantly differ in terms of gender and age, and most patients recovered within 12 weeks.
Article
Multidisciplinary Sciences
Hana Kiyama, Shigeyuki Kakizawa, Yuya Sasajima, Yuhei O. Tahara, Makoto Miyata
Summary: This study reveals that the differentiation and coupling of bacterial actins are crucial for the origin of motility and successfully achieves motility in a synthetic minimal bacterium.
Article
Biochemistry & Molecular Biology
Carolin Wevers, Mona Hoehler, Abel R. Alcazar-Roman, Johannes H. Hegemann, Ursula Fleig
Summary: Bacterial pathogens have evolved complex mechanisms to manipulate their hosts and support infection. This study systematically assessed the importance of the microtubule cytoskeleton in Chlamydiae infection. The elimination of microtubules significantly reduced the efficiency of C. pneumoniae infection, indicating the essential role of microtubules in the early stages of infection. A screen in yeast identified multiple chlamydial proteins that modulate microtubule dynamics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Ram Prasad Awal, Frank D. Mueller, Daniel Pfeiffer, Caroline L. Monteil, Guy Perriere, Christopher T. Lefevre, Dirk Schueler
Summary: This study investigated the ability of magnetosome-associated proteins from different MTB to rescue magnetoskeleton mutants of the model Magnetospirillum gryphiswaldense. The results showed that MamK orthologs from various MTB could partially restore magnetosome assembly and exhibit filamentous localization. A novel magnetosome-related protein was also identified, which could substitute the function of the well-characterized MamJ protein. Additionally, it was demonstrated that Mad28 orthologs are actin-like proteins that can complement mamK mutants and form filamentous structures.
Article
Biochemistry & Molecular Biology
Daichi Takahashi, Ikuko Fujiwara, Yuya Sasajima, Akihiro Narita, Katsumi Imada, Makoto Miyata
Summary: MreB is a bacterial protein involved in cell wall synthesis and cell shape determination. This study investigates the structure and dynamics of MreB homologs in Spiroplasma eriocheiris. The results reveal different polymerization behaviors and the importance of a specific amino acid motif in the hydrolysis of ATP.
Review
Biochemistry & Molecular Biology
Saif S. Alqassim
Summary: The actin cytoskeleton is essential for cellular processes, and various pathogens manipulate the infected host's actin dynamics for their own benefit. Understanding the mechanisms by which pathogens interact with and control actin assembly can provide insights into fundamental determinants of actin assembly and host-pathogen interactions, and contribute to therapeutic development efforts. This review focuses on the shared and unique features of pathogen effectors that directly bind and assemble actin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Qi Zhao, Jiaqi Yan, Jingjing Wang, Ruihua Liu, Mark Bartlam
Summary: Iron acquisition is crucial for cell physiology in biological systems. The study reveals the crystal structure of KfuA in Klebsiella pneumoniae and demonstrates its ability to bind with ferric ions and gallium. Gallium inhibits bacterial growth, while ferric iron enhances it, suggesting that gallium competes with ferric iron and disrupts iron-dependent biological functions by binding to KfuA, leading to increased antimicrobial efficacy. Furthermore, the absence of equivalent ABC transporters in humans highlights the potential of KfuA as a promising target for therapeutic intervention.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biology
Addison Grinnell, Ryan Sloan, Randy M. Morgenstein
Summary: A new mechanism of antibiotic tolerance in rod-shaped bacteria that is dependent on cell density and specifically targets the cell wall elongation system has been reported. The findings suggest that disrupting both the elongasome and the divisome may be an efficient way of overcoming density-dependent growth resistance.
COMMUNICATIONS BIOLOGY
(2022)
Article
Immunology
Huimin Chen, Shuan Tao, Na Li, Fang Wang, Lei Wang, Yu Tang, Wei Liang
Summary: Research findings show that plasmid sequence and restriction proteins impact gene transfer and confirm the anti-restriction and anti-methylation activities of ArdA and KlcA during transformation and transduction. ArdA shows stronger effects, while KlcA only exhibits anti-methylation during transformation. Understanding the molecular mechanism of clinical dissemination of K. pneumoniae and other resistant strains from the perspective of restrictive and anti-restrictive systems provides theoretical support for prevention and control of multidrug-resistant bacteria, as well as new strategies for delaying or controlling the clinical dissemination of resistant strains in the future.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Bhagyashree Gundagurti, Papa Dasari, Rakesh Singh
Summary: This study showed a significant association between Chlamydophila pneumoniae infection and hypertension during pregnancy, with a higher detection rate of C. pneumoniae DNA in the placenta of women with hypertension compared to normotensive women. Elevated levels of HSP-60 were observed in women with preeclampsia, while IgA antibodies were not detected and IgG antibodies were positive in a subset of women with preeclampsia. Further studies are warranted to confirm the causative role of C. pneumoniae in pregnancy-related hypertension.
CLINICAL AND EXPERIMENTAL HYPERTENSION
(2021)
Article
Biology
Maryam Izadi, Eric Seemann, Dirk Schlobinski, Lukas Schwintzer, Britta Qualmann, Michael M. Kessels
Summary: The development of dendritic arbors in neurons depends on the coordination of different actin formation-promoting factors, including Cobl, Cobl-like, and syndapins. These proteins work together through physical and chemical interactions to regulate local actin dynamics, ultimately leading to the formation of complex neuronal morphologies required for neuronal network formation in mammals.
Article
Cell Biology
Jingqi Wang, Maciej Daniszewski, Marlene M. Hao, Damian Hernandez, Alice Pebay, Paul A. Gleeson, Lou Fourriere
Summary: This study investigates the secretory pathways within dendrites of human neurons. It finds that the entire Golgi apparatus transiently translocates into dendrites during early neuronal development. In mature neurons, dynamic Golgi structures are transported along dendrites, showing bidirectional movement. This study reveals the presence of dynamic, functional Golgi structures in dendrites and provides insights into dendrite trafficking in human neurons.
Review
Microbiology
Iveta Vojtechova, Tomas Machacek, Zdenka Kristofikova, Ales Stuchlik, Tomas Petrasek
Summary: The amyloid cascade hypothesis has not been able to uncover the root cause of Alzheimer's disease (AD) or provide effective therapy. The emerging infectious hypothesis suggests that CNS infection is the primary trigger of sporadic AD, and amyloid beta may act as an antimicrobial peptide. This review discusses the evidence for the involvement of infections in AD and proposes that targeting amyloid beta alone would be ineffective in AD treatment.
Article
Parasitology
Naglaa Fathi Abd El-Latif, Hend Aly El-Taweel, Ahmed Gaballah, Aziza Ibrahim Salem, Ahmed Hamdi Mohamed Abd El-Malek
ACTA PARASITOLOGICA
(2020)
Article
Infectious Diseases
Aliaa Aboulela, Eglal El-Sherbini, Ghada Abu-Sheasha, Hala Abd El-Raouf, Ebtesam El-Ghazzawi, Ahmed Gaballah
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2020)
Article
Nanoscience & Nanotechnology
Salma El-Habashy, Hoda Eltaher, Ahmed Gaballah, Radwa Mehanna, Amal H. El-Kamel
Summary: This study successfully developed a novel composite nanoparticle system that integrates doxycycline into biomaterials to improve its cytotoxicity and enhance osteogenic activity. The experimental results showed that this composite system can control the release of doxycycline and significantly ameliorate its toxicity to bone marrow stem cells, while promoting the maturation of osteogenic cells.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Materials Science, Biomaterials
Salma E. El-Habashy, Hoda M. Eltaher, Ahmed Gaballah, Eiman I. Zaki, Radwa A. Mehanna, Amal H. El-Kamel
Summary: Hybrid biomaterial-based hydroxyapatite/polycaprolactone nanoparticles (HAp/PCL NPs) were prepared to enhance osteogenicity, showing superior performance in mesenchymal cell proliferation and osteodifferentiation with reduced cytotoxicity. The hybrid NPs also exhibited moderate expression of early osteogenic markers and significantly elevated expression of late osteogenic markers, suggesting a promising potential for bone regenerative applications.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Immunology
Ahmed Noby Amer, Ahmed Gaballah, Rasha Emad, Abeer Ghazal, Nancy Attia
Summary: The study on HIV-1 subtyping among Egyptian patients revealed that non-B subtypes are predominant, with CRF02_AG being the most common subtype. This suggests a different distribution of HIV subtypes in Egypt compared to other regions.
CURRENT HIV RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Mohamed G. El-Melegy, Hoda M. Eltaher, Ahmed Gaballah, Amal H. El-Kamel
Summary: This study developed T-R-loaded nanocochleates with promising anticancer activity, demonstrating good performance in in-vitro evaluations.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Abdul Seguya, Mohamed Mowafy, Ahmed Gaballah, Abbas Zaher
Summary: This study compared the efficacy of chlorhexidine diaceteate varnish and an organoselenium sealant in preventing S. mutans biofilm formation on human teeth. The results showed that both materials were not effective in inhibiting S. mutans biofilm attachment.
Article
Virology
Ahmed Gaballah, Abeer Ghazal, Dalia Metwally, Rasha Emad, Ghada Essam, Nancy M. Attia, Ahmed N. Amer
Summary: The aim of this study was to detect the prevalence of drug resistance mutations related to HIV treatment. By analyzing the sequences of HIV-1 samples, several common mutations were identified. These findings provide important initial information for understanding the overall prevalence of HIV-1 drug resistance in Egypt.
Article
Medicine, General & Internal
Ahmed Gaballah, Abeer Ghazal, Reda Almiry, Rasha Emad, Nadia Sadek, Mohamed Abdel Rahman, Eglal El-Sherbini
Summary: This study revealed the presence of Mycobacterium tuberculosis complex and several atypical mycobacterial species among immunocompromised patients in Egypt using real-time PCR and DNA-microarray techniques.
MEDICAL PRINCIPLES AND PRACTICE
(2022)
Article
Microbiology
Ahmed Gaballah, Abeer Ghazal, Reda Almiry, Somaya Hussein, Rasha Emad, Eglal El-Sherbini
Summary: This study aimed to evaluate the transmission patterns of Mycobacterium tuberculosis strains in Alexandria, Egypt using MIRU-VNTR genotyping technique. The results revealed a low clustering rate and a low recent transmission rate of M. tuberculosis strains. Additionally, the S family was found to be the dominant lineage among the isolates.
JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Review
Oncology
Ahmed Gaballah, Birke Bartosch
Summary: Cancer cells and cells infected with oncogenic viruses both exhibit metabolic changes, including increased nutritional needs and altered metabolism. Studies have found that there are many similarities in the metabolic alterations observed in various types of cancers and in cells infected with oncogenic viruses. These shared characteristics suggest that metabolic inhibitors used for cancer treatment can also be targeted for antiviral therapy.
Article
Gastroenterology & Hepatology
Sarah Duponchel, Lea Monnier, Jennifer Molle, Nadia Bendridi, Muhammad Rizwan Alam, Ahmed Gaballah, Boyan Grigorov, Alexander Ivanov, Marcel Schmiel, Margarete Odenthal, Michel Ovize, Jennifer Rieusset, Fabien Zoulim, Birke Bartosch
Summary: Chronic HCV infection causes cellular stress, fibrosis, and predisposes to hepatocarcinogenesis. Mitochondria play key roles in orchestrating stress responses. The study revealed that HCV proteins associate with mitochondria and alter the composition of mitochondrial subdomains, which are crucial for efficient viral replication.
Article
Microbiology
Ahmed Gaballah, Ghada Hani Ali, Rasha Emad, Hoda Omar, Hamida Moustafa Abou-Shleib
Summary: This study aimed to investigate the phenotypic and genotypic characteristics of clinical isolates of Klebsiella pneumoniae in Alexandria, Egypt. The majority of isolates demonstrated resistance to multiple antibiotics, with the most common resistance genes being bla(SHV) and bla(CTX-Mu). The predominant mechanisms of antibiotic resistance among these isolates were ESBL and carbapenemase production. Additionally, it was found that resistance to cefoxitin among the isolates was primarily not mediated by AmpC production.
CURRENT MICROBIOLOGY
(2023)
Article
Virology
Ahmed Gaballah, Iman Salah Naga, Mariam Salah Zaghloul, Hanan Mostafa Mostafa, Ahmed Noby
Summary: Direct acting antiviral agents (DAAs) inhibit specific non-structural proteins of the virus to disrupt viral replication and infection, providing insight into the relationship between innate immunity and HCV. IL-29 prevents HCV replication, while IP-10 plays a role in HCV pathogenesis. The co-expression of IL-29, IP-10, and MIG was significantly increased in untreated chronic HCV patients and decreased in those who achieved sustained viral response (SVR) after successful DAAs therapy.
Article
Parasitology
Hend A. El-Taweel, Yasmine A. Isaa, Ghada A. Shehata, Ahmed Gaballah, Wael M. Lotfy, Mona M. Tolba
PARASITOLOGISTS UNITED JOURNAL
(2020)