Article
Biotechnology & Applied Microbiology
Nimalan Jeyagowri, Chaminda Senaka Ranadheera, Mohd Yazid Manap, Ashoka Gamage, Othmane Merah, Terrence Madhujith
Summary: The study identified and characterized lactic acid bacteria (LAB) from fermented rice varieties. Five species of LAB were isolated with potential probiotic characteristics. The isolated LAB exhibited beneficial physiological and biochemical properties, indicating their potential applications in the food industry. Further studies are recommended to confirm their probiotic properties and health benefits.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Sadhana Singh, Himabindu Kudapa, Vanika Garg, Rajeev K. Varshney
Summary: This study identified a total of 21 potential drought-responsive NAC genes in chickpea, pigeonpea, and groundnut, which showed correlation between gene expression, transcriptional regulation, and better tolerance against drought. These candidate genes could be useful for molecular breeding for drought tolerance in legume crops in semi-arid tropics.
Review
Biotechnology & Applied Microbiology
Shiman Jiang, Lingzhi Cai, Longxian Lv, Lanjuan Li
Summary: Pediococcus pentosaceus, a promising strain of lactic acid bacteria, has advantages such as improving taste and nutrition of food, as well as antimicrobial abilities. Further study of P. pentosaceus strains is needed to clarify their benefits and drawbacks for future applications.
MICROBIAL CELL FACTORIES
(2021)
Article
Plant Sciences
Ritu Singh, Ashutosh Pandey, Praveen Kumar Verma
Summary: In this study, the GARP gene family in three major leguminous plants was explored from genomic, molecular, and evolutionary perspectives. The results showed the conservation of the GARP gene family in plants and the expansion through genome duplication in legumes. The GARP genes were found to play a major role in signaling, growth, development, and stress processes. The study also provided stress-responsive genes and laid the foundation for prospective functional studies of GARPs in legumes.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Nutrition & Dietetics
Chengeshpur Anjali Goud, Vanisri Satturu, Renuka Malipatil, Aswini Viswanath, Janani Semalaiyappan, Himabindu Kudapa, Santosha Rathod, Abhishek Rathore, Mahalingam Govindaraj, Nepolean Thirunavukkarasu
Summary: Pearl millet, an important source of iron and zinc for dryland tropics, has been studied to identify key genes and pathways related to mineral pathways under stress conditions. The results provide valuable insights for developing biofortified pearl millet varieties and improving nutrition in South Asia and Sub-Saharan Africa.
FRONTIERS IN NUTRITION
(2022)
Review
Chemistry, Analytical
Salvatore Caterino, Nicolo Pajer, Claudia Crestini
Summary: The empirical exploitation of the interaction between polyphenols and iron for the production of iron-gall inks has been used since the XII century. These inks, widely used in Europe and the Middle East for manuscript production, are part of the cultural heritage and require careful preservation. This paper reviews the current knowledge on iron-gall inks, including the ingredients used, models describing complex formation, degradation patterns, and available instrumental techniques for their characterization.
MICROCHEMICAL JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Huan Wang, Jia Jia, Zhandong Cai, Mingming Duan, Ze Jiang, Qiuju Xia, Qibin Ma, Tengxiang Lian, Hai Nian
Summary: Research on the genetic mechanism of seed iron (Fe) and zinc (Zn) content in soybean is important for addressing global hidden hunger. In this study, a population of recombinant inbred lines (RILs) was evaluated in four environments to identify quantitative trait loci (QTLs) associated with seed Fe and Zn content. The results showed significant variation in seed Fe and Zn content across environments, with strong influences of genotype, environment, and genotype x environment interactions. The study also discovered QTLs and candidate genes related to seed Fe and Zn content, which can contribute to the biofortification of soybeans and accelerate the development of marker-assisted selection (MAS) for breeding iron and zinc-fortified soybeans.
Review
Plant Sciences
B. P. Mallikarjuna Swamy, Balram Marathi, Ana I. F. Ribeiro-Barros, Mark Ian C. Calayugan, Felipe Klein Ricachenevsky
Summary: Rice is the most suitable model species for studying Fe homeostasis and biofortification, with over 90 Fe QTLs identified and multiple genes associated with Fe translocation and accumulation. Current research focuses on the transport, accumulation, and distribution of Fe in rice grains.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Microbiology
Guoqing Zhang, Ning Yang, Zhongyuan Liu, Xinyu Chen, Mengjiao Li, Tongyu Fu, Donghong Zhang, Cuiqing Zhao
Summary: This comprehensive study focused on the genetic analysis of Lactiplantibacillus plantarum 18 (LP18) to understand its survival and colonization characteristics in the gastrointestinal tract. Supplementing LP18 in the diet of laying hens resulted in significant improvements in growth performance, egg quality, and lipid metabolism. The study revealed specific genes associated with adaptability, adhesion, and gastrointestinal safety in LP18. These findings highlight the potential of LP18 as a probiotic candidate to enhance productivity, optimize egg quality, and modulate lipid metabolism in poultry production.
Article
Biochemical Research Methods
Amanda Yunn Ee Ng, Jun Wei Pek
Summary: Stable Intronic Sequence RNA (sisRNA) is a relatively new class of non-coding RNA that plays regulatory roles in gene expression through feedback loops. Identification of sisRNA has been challenging due to its low abundance, but a specific workflow can reliably detect and validate the presence of circular sisRNA.
Review
Biotechnology & Applied Microbiology
D. Cheng, M. Z. Xie
Summary: Akkermansia muciniphila, a common colonizer in the human intestinal mucus layer, is considered a promising candidate for the next-generation probiotic due to its physiological benefits. However, current barriers such as regulations, large-scale clinical experiments, and production feasibility need to be addressed before it can be widely applied as a probiotic.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Nutrition & Dietetics
Nefertiti OjiNjideka Hemphill, Lacey Pezley, Alana Steffen, Gloria Elam, Michelle A. Kominiarek, Angela Odoms-Young, Nicollette Kessee, Alyshia Hamm, Lisa Tussing-Humphreys, Mary Dawn Koenig
Summary: This study aims to assess the effect of Lp299v on maternal and neonatal iron status during pregnancy, and the results indicate that Lp299v may have positive effects on the hematological and iron status of pregnant individuals and their newborns.
Article
Multidisciplinary Sciences
Jia Wei, Bo Luo, Shiyi Kong, Wenxian Liu, Chuanjie Zhang, Zhenwu Wei, Xueyang Min
Summary: In this study, a comprehensive transcriptome analysis of common vetch under multiple abiotic stress treatments was conducted using hybrid-sequencing technology. Various important genetic elements, such as alternative splicing events, simple sequence repeats, and putative transcription factors, were identified. Additionally, long noncoding RNAs and complete coding sequences were also discovered. Through gene expression, annotation information, and protein-protein interaction networks, seven candidate genes responsive to multiple abiotic stresses were obtained. This research not only enriched the understanding of common vetch's transcriptome, but also provided valuable insights into the molecular mechanism of stress tolerance in this and related leguminous plants.
Article
Microbiology
Yogini Dixit, Khushboo Kanojiya, Namrata Bhingardeve, Jayesh J. Ahire, Dina Saroj
Summary: In this study, the production, characterization, and identification of antimicrobial compounds from Alkalihalobacillus clausii 088AE were evaluated. The results demonstrated that the supernatant of 48-h-old A. clausii can inhibit the growth of Micrococcus luteus (7.0 & PLUSMN; 0.51 mm). Enhanced antimicrobial activity (19.67 & PLUSMN; 0.58 mm) against indicator strain was observed after cultivating 088AE on solid media with XAD16N beads for 5 days and extracting the antimicrobial compound with isopropanol. The purified compound, clausin, showed stability in various conditions and antimicrobial activity against Gram-positive pathogens.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Microbiology
Zhen -Ping Zou, Yue Du, Ting -Ting Fang, Ying Zhou, Bang -Ce Ye
Summary: Engineered probiotic i-ROBOT has been developed to noninvasively monitor and record the occurrence and progression of inflammatory bowel disease (IBD) in real time, and release treatments based on disease-associated biomarkers. It provides a heritable genomic DNA sequence and a colorimetric signal by activating a base-editing system in response to the inflammatory marker thiosulfate. In experiments with mice, i-ROBOT effectively ameliorated colitis and showed potential for the treatment of gastrointestinal and metabolic disorders.
CELL HOST & MICROBE
(2023)
Review
Gastroenterology & Hepatology
Stamatia Papoutsopoulou, Jack Satsangi, Barry J. Campbell, Chris S. Probert
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
(2020)
Article
Gastroenterology & Hepatology
Nicholas A. Kennedy, Gareth-Rhys Jones, Christopher A. Lamb, Richard Appleby, Ian Arnott, R. Mark Beattie, Stuart Bloom, Alenka J. Brooks, Rachel Cooney, Robin J. Dart, Cathryn Edwards, Aileen Fraser, Daniel R. Gaya, Subrata Ghosh, Kay Greveson, Richard Hansen, Ailsa Hart, A. Barney Hawthorne, Bu'Hussain Hayee, Jimmy K. Limdi, Charles D. Murray, Gareth C. Parkes, Miles Parkes, Kamal Patel, Richard C. Pollok, Nick Powell, Chris S. Probert, Tim Raine, Shaji Sebastian, Christian Selinger, Philip J. Smith, Catherine Stansfield, Lisa Younge, James O. Lindsay, Peter M. Irving, Charlie W. Lees
Article
Gastroenterology & Hepatology
Jonathan M. Rhodes, Sreedhar Subramanian, Paul K. Flanagan, Graham W. Horgan, Kate Martin, John Mansfield, Miles Parkes, Ailsa Hart, Helen Dallal, Tariq Iqbal, Jeffrey Butterworth, Kate Culshaw, Christopher Probert
Summary: The study assessed the efficacy and safety of an antibiotic/hydroxychloroquine combination for Crohn's disease, finding overall results to be unimpressive but showing long-term remission in some patients, warranting further study.
DIGESTIVE DISEASES AND SCIENCES
(2021)
Article
Gastroenterology & Hepatology
Shahida Din, Alexandra Kent, Richard C. Pollok, Susanna Meade, Nicholas A. Kennedy, Ian Arnott, R. Mark Beattie, Felix Chua, Rachel Cooney, Robin J. Dart, James Galloway, Daniel R. Gaya, Subrata Ghosh, Mark Griffiths, Laura Hancock, Richard Hansen, Ailsa Hart, Christopher Andrew Lamb, Charlie W. Lees, Jimmy K. Limdi, James O. Lindsay, Kamal Patel, Nick Powell, Charles D. Murray, Chris Probert, Tim Raine, Christian Selinger, Shaji Sebastian, Philip J. Smith, Phil Tozer, Andrew Ustianowski, Lisa Younge, Mark A. Samaan, Peter M. Irving
Article
Cell Biology
Katie Lloyd, Stamatia Papoutsopoulou, Emily Smith, Philip Stegmaier, Francois Bergey, Lorna Morris, Madeleine Kittner, Hazel England, Dave Spiller, Mike H. R. White, Carrie A. Duckworth, Barry J. Campbell, Vladimir Poroikov, Vitor A. P. Martins dos Santos, Alexander Kel, Werner Muller, D. Mark Pritchard, Chris Probert, Michael D. Burkitt
DISEASE MODELS & MECHANISMS
(2020)
Letter
Gastroenterology & Hepatology
Thomas E. Conley, Chris Probert, Sreedhar Subramanian
JOURNAL OF CROHNS & COLITIS
(2020)
Article
Health Care Sciences & Services
Pamela Qualter, Alison Rouncefield-Swales, Lucy Bray, Lucy Blake, Steven Allen, Chris Probert, Kay Crook, Bernie Carter
Summary: The study found that disease severity and abdominal pain in IBD patients have a negative impact on their mental health, while embarrassment can increase depression, anxiety, and loneliness. Discussion of mental health should be an important part of clinical dialogue, considering how perceived stigma affects those with chronic illness.
QUALITY OF LIFE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Prashant Aggarwal, James Baker, Mark T. Boyd, Seamus Coyle, Chris Probert, Elinor A. Chapman
Article
Agriculture, Dairy & Animal Science
Rachael Slater, Alessandra Frau, Jane Hodgkinson, Debra Archer, Chris Probert
Summary: Anoplocephala perfoliata infection in horses is associated with an increased risk of colic, with potential differences in the intestinal microbiome and metabolome between infected and non-infected horses. VOCs may have the potential to serve as markers for bacterial OTUs in equine colonic contents.
Article
Biochemistry & Molecular Biology
Awad Mahalhal, Michael D. Burkitt, Carrie A. Duckworth, Georgina L. Hold, Barry J. Campbell, David Mark Pritchard, Chris S. Probert
Summary: Long-term alterations in dietary iron affect gut microbiota signatures but do not exacerbate chronic colitis, while exacerbating acute colitis. More research is needed to understand the impact of iron supplementation on inflammatory bowel disease. The change in microbiome in colitis patients may be due to increased luminal iron, rather than the colitis itself.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Alessandra Frau, Lauren Lett, Rachael Slater, Gregory R. Young, Christopher J. Stewart, Janet Berrington, David M. Hughes, Nicholas Embleton, Chris Probert
Summary: The fecal metabolome in early life of pre-term babies was studied through analysis of stool samples using VOCs analysis. The metabolome shifted significantly 5 days after birth, coinciding with the establishment of enteral feeding and transition from meconium to feces. Prior to this shift, aldehydes and acetic acid dominated the metabolome, with a rise in branched-chain fatty acids observed. Additionally, caesarean delivery showed a modest association with molecules of fungal origin.
Article
Nutrition & Dietetics
Awad Mahalhal, Alessandra Frau, Michael D. Burkitt, Umer Z. Ijaz, Christopher A. Lamb, John C. Mansfield, Stephen Lewis, D. Mark Pritchard, Chris S. Probert
Summary: This study demonstrates differential and unique influences of ferric maltol and ferrous sulphate supplements on intestinal microbiota. FS treatment led to an increase in five genera, but FM was not associated with any measurable change. The severity of DSS-induced colitis was greater with FSS than FMS.
Article
Medicine, General & Internal
Ayesha Akbar, Ian Arnott, Nicholas A. Kennedy, Jonathan Nolan, Simon Peake, Simon R. Whiteoak, Chris Probert, Aileen Fraser, Alex Cheshire, Allyson Lewis, Kathleen Sugrue, Susan Laird, Glyn Scott
Summary: The 2021 National report from IBD UK revealed that over 70% of patients with inflammatory bowel disease experienced at least one flare in the past year. Mesalazine's action and delivery mechanisms are crucial for managing mild and moderate ulcerative colitis, but ensuring patient concordance and adherence remains a challenge for healthcare professionals. Recommendations for personalized mesalazine therapy before escalating to other treatments were discussed in a roundtable involving gastroenterology consultants and specialist nurses.
BRITISH JOURNAL OF HOSPITAL MEDICINE
(2021)
Article
Gastroenterology & Hepatology
Alessandra Frau, Umer Z. Ijaz, Rachael Slater, Daisy Jonkers, John Penders, Barry J. Campbell, John G. Kenny, Neil Hall, Luca Lenzi, Michael D. Burkitt, Marieke Pierik, Alistair C. Darby, Christopher S. J. Probert
Summary: The etiology of Crohn's disease involves bacterial and fungal microbiota, with dysbiosis observed in CD patients. Bacteroides may play a key role in CD and modulate Candida colonization. Different medications lead to varying changes in the fungal community, with Malassezia and Candida increasing in immunosuppressant-treated patients.
Article
Gastroenterology & Hepatology
Andrew Nelson, Christopher J. Stewart, Nicholas A. Kennedy, John K. Lodge, Mark Tremelling, Chris S. Probert, Miles Parkes, John C. Mansfield, Darren L. Smith, Georgina L. Hold, Charlie W. Lees, Simon H. Bridge, Christopher A. Lamb
Summary: Comparative analysis of fecal mycobiota in patients with CD and non-CD subjects revealed compositional changes in the gut mycobiota of CD patients, suggesting a potential role of fungi in the pathogenesis of CD. No NOD2 genotype-specific differences were observed in the fecal mycobiota.
JOURNAL OF CROHNS & COLITIS
(2021)