Article
Biochemical Research Methods
Roberto Rodriguez-Moncayo, Diana F. Cedillo-Alcantar, Pablo E. Guevara-Pantoja, Oriana G. Chavez-Pineda, Jose A. Hernandez-Ortiz, Josue U. Amador-Hernandez, Gustavo Rojas-Velasco, Fausto Sanchez-Munoz, Daniel Manzur-Sandoval, Luis D. Patino-Lopez, Daniel A. May-Arrioja, Rosalinda Posadas-Sanchez, Gilberto Vargas-Alarcon, Jose L. Garcia-Cordero
Summary: The applications of serology tests for SARS-CoV-2 include diagnosing COVID-19, understanding humoral responses, estimating prevalence, and modeling the pandemic. A new high-throughput microfluidic device can assess antibody reactivities to four SARS-CoV-2 antigens from 50 serum samples simultaneously, with high sensitivity and specificity.
Article
Nanoscience & Nanotechnology
Xuan Luo, Wenjin Xing, Iliana Delcheva, Fayed Abdullah Alrashaidi, Amir Heydari, Dennis Palms, Vi Khanh Truong, Krasimir Vasilev, Zhongfan Jia, Wei Zhang, Peng Su, Kasturi Vimalanathan, Aghil Igder, Gregory A. Weiss, Youhong Tang, Melanie MacGregor, Colin L. Raston
Summary: A shear-stress-mediated platform called portable vortex-fluidic device (P-VFD) has been developed to overcome the diffusion-limit and achieve fast reaction rates. The platform consists of a plasma oxazoline-coated polyvinyl chloride (POx-PVC) film with tough hydrogel covalently bound to its surface and a reactor tube for reactions. The hydrogel arrays on the film provide a strong matrix for entrapping biomolecules and tolerate shear stress, resulting in a >6-fold increase in reaction rate.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Gregory J. Hunt, Mark A. Dane, James E. Korkola, Laura M. Heiser, Johann A. Gagnon-Bartsch
Summary: In this study, we propose a general approach that leverages an experimental design with multiple levels of replication to remove technical artifacts from high-throughput image data. By applying this approach to microenvironment microarrays (MEMAs), we are able to eliminate unwanted spatial artifacts and enhance the biological signal. This method has broad applicability in various biological assays.
Article
Chemistry, Applied
Ines Fuster Fernandez, Michael Kickstein, Wolf-Dieter Fessner
Summary: This article reports the generation of metal chelating compounds by the transketolase from Geobacillus stearothermophilus, which can be used for high-throughput screening of TKgst and its variants. The colorimetric assay in both solid- and liquid-phase formats validated the specificity and sensitivity of the assays. This method is important for engineering the TKgst enzyme towards improved conversion of aromatic aldehydes.
ADVANCED SYNTHESIS & CATALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Ana Maria Gracioso Martins, Michael D. Wilkins, Frances S. Ligler, Michael A. Daniele, Donald O. Freytes
Summary: The study proposes an inexpensive, versatile, high-throughput platform for measuring the contraction of microtissues by embedding optical fiber microprobes. This platform accurately quantifies positional changes in optical microprobes and can be used for high-throughput screening of microtissues in disease modeling, drug screening, physiology research, and safety pharmacology.
Article
Biochemistry & Molecular Biology
Mahsa Karbaschi, Yunhee Ji, Mubarak A. Mujawar, Mario Mendoza, Juan S. Marquez, Apurva Sonawane, Pratikkumar Shah, Chris Ross, Shekhar Bhansali, Marcus S. Cooke
Summary: This article describes the development of an automated device for high throughput sample processing in a comet assay, which reliably assesses DNA damage in individual eukaryotic cells. The device performs as well as manual methods but with the advantages of decreased human involvement and assay run time, making it a valuable approach for high throughput DNA damage assessment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Duncan McCloskey, Juan Boza, Christopher E. Mason, David Erickson
Summary: The research has developed a high throughput device called MINI that can test hundreds of samples per day on-site, potentially increasing access to modern molecular diagnostics in limited resource settings and improving health outcomes.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Minghui Tang, Xin Duan, Anqi Yang, Shijie He, Yajing Zhou, Yuxin Liu, Lu Zhang, Xuan Luo, Peng Shi, Honglin Li, Xudong Lin
Summary: This study describes a fish capsule system that can be used for large-scale functional screening of fish, combined with real-time microscopy. This system allows for rapid screening and analysis of drugs, accelerating the discovery of new therapeutics.
Article
Cell Biology
E. J. S. Denessen, D. L. Van den Kerkhof, M. L. J. Jeurissen, R. J. H. Wetzels, P. W. M. Verhezen, Y. M. C. Henskens
Summary: Platelet function assays and global haemostasis assays are crucial in diagnosing bleeding tendencies. Pre-analytical variables, like storage time and temperature during transport, are considered vulnerable and may affect test results. Our data showed that PFA whole blood can be stored for 3 hours at room temperature, while Multiplate and ROTEM whole blood can be stored for 6 hours. LTA was stable for up to 3 hours at 4 degrees C or room temperature, and 6 hours at room temperature.
Review
Pharmacology & Pharmacy
Chelsea B. Pratt, Matt Alexander
Summary: The development of CAR-T cells is crucial for the success of adoptive T-cell immunotherapy. While the process of producing autologous CAR-T cells is time-consuming and expensive, the exploration of cost-effective allogeneic CAR-T cells is essential. High-throughput tools are used to monitor the entire process of CAR-T cell design, testing, manufacturing, and patient monitoring to ensure safety and efficacy.
DRUG DISCOVERY TODAY
(2022)
Article
Agriculture, Multidisciplinary
Angela Puig-Sirera, Lorenzo Bonzi, Lorenzo Cotrozzi, Fatma Hamouda, Alessandra Marchica, Giuseppe Provenzano, Damiano Remorini, Giovanni Rallo
Summary: Climate change leads to more frequent and intense abiotic stresses causing water scarcity. Breeding plants tolerant to water stress and adapted to future conditions is necessary. This study introduces a High-Throughput Screening system to continuously monitor plant stress response to drought in a semi-controlled environment. The system combines gravimetric weighing, soil moisture, and atmospheric sensors.
COMPUTERS AND ELECTRONICS IN AGRICULTURE
(2023)
Article
Biology
Srikanya Kundu, Molly E. Boutin, Caroline E. Strong, Ty Voss, Marc Ferrer
Summary: Fibrin gel-based 3D co-culture models with human-iPSC differentiated neurons and astrocytes, combined with biosensors and optogenetics activation, enable the creation of functional neural models for drug response studies.
COMMUNICATIONS BIOLOGY
(2022)
Review
Chemistry, Analytical
Qiang Jin, Li-Lin Song, Le-Le Ding, Jing Zhang, Dan-Dan Wang, Yun-Qing Song, Li-Wei Zou, Guang-Bo Ge
Summary: This review summarizes the latest advances in the design and development of specific optical substrates for mammalian SHs and related high-throughput optical assays, as well as their applications in bioanalytical and biomedical fields.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Mingu Gordon Park, Ah-reum Han, Su Yeon Kim, Tai Young Kim, Ho Min Kim, C. Justin Lee
Summary: This study introduces an optimized method for overexpressing 6xHis-tagged human GABA-T in human cells and establishing an activity assay, providing insights into the sensitivity differences between human and bacterial GABA-T to inhibitory compounds.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Marshall S. Ma, Subramanian Sundaram, Lihua Lou, Arvind Agarwal, Christopher S. Chen, Thomas G. Bifano
Summary: Three dimensional engineered cardiac tissues (3D ECTs) are crucial in vitro models for assessing drug cardiotoxicity, but the low throughput of current assays limits their efficiency. To overcome this limitation, an innovative mosaic imaging system was developed to measure contractile force of 3D ECTs seeded on a 96-well plate, increasing throughput to 96 samples per measurement.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biophysics
Rachel T. Wragg, Geraldine Gouzer, Jihong Bai, Gianluca Arianna, Timothy A. Ryan, Jeremy S. Dittman
BIOPHYSICAL JOURNAL
(2015)
Article
Cell Biology
Rajarshi Chakrabarti, Wei-Ke Ji, Radu V. Stan, Jaime de Juan-Sanz, Timothy A. Ryan, Henry N. Higgs
JOURNAL OF CELL BIOLOGY
(2018)
Article
Neurosciences
Ping-Yue Pan, Xianting Li, Jing Wang, James Powell, Qian Wang, Yuanxi Zhang, Zhaoyu Chen, Bridget Wicinski, Patrick Hof, Timothy A. Ryan, Zhenyu Yue
JOURNAL OF NEUROSCIENCE
(2017)
Editorial Material
Neurosciences
Timothy A. Ryan
NATURE NEUROSCIENCE
(2018)
Article
Neurosciences
Jaime de Juan-Sanz, Graham T. Holt, Eric R. Schreiter, Fernando de Juan, Douglas S. Kim, Timothy A. Ryan
Article
Neurosciences
Ghazaleh Ashrafi, Zhuhao Wu, Ryan J. Farrell, Timothy A. Ryan
Article
Pharmacology & Pharmacy
Lisa M. Weatherly, Andrew J. Nelson, Juyoung Shim, Abigail M. Riitano, Erik D. Gerson, Andrew J. Hart, Jaime de Juan-Sanz, Timothy A. Ryan, Roger Sher, Samuel T. Hess, Julie A. Gosse
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2018)
Review
Neurosciences
Jeremy S. Dittman, Timothy A. Ryan
NATURE REVIEWS NEUROSCIENCE
(2019)
Article
Neurosciences
Frank Koopmans, Pim van Nierop, Maria Andres-Alonso, Andrea Bymes, Tony Cijsouw, Marcelo P. Coba, L. Niels Cornelisse, Ryan J. Farrell, Hana L. Goldschmidt, Daniel P. Howrigan, Natasha K. Hussain, Cordelia Imig, Arthur P. H. de Jong, Hwajin Jung, Mandokht Kohansalnodehi, Barbara Kramarz, Noa Lipstein, Ruth C. Lovering, Harold MacGillavry, Vittoria Mariano, Huaiyu Mi, Momchil Ninov, David Osumi-Sutherland, Rainer Pielot, Karl-Heinz Smalla, Haiming Tang, Katherine Tashman, Ruud F. G. Toonen, Chiara Verpelli, Rita Reig-Viader, Kyoko Watanabe, Jan van Weering, Tilmann Achsel, Ghazaleh Ashrafi, Nimra Asi, Tyler C. Brown, Pietro De Camilli, Marc Feuermann, Rebecca E. Foulger, Pascale Gaudet, Anoushka Joglekar, Alexandros Kanellopoulos, Robert Malenka, Roger A. Nicoll, Camila Pulido, Jaime de Juan-Sanz, Morgan Sheng, Thomas C. Sudhof, Hagen U. Tilgner, Claudia Bagni, Alex Bayes, Thomas Biederer, Nils Brose, John Jia En Chua, Daniela C. Dieterich, Eckart D. Gundelfinger, Casper Hoogenraad, Richard L. Huganir, Reinhard Jahn, Pascal S. Kaeser, Eunjoon Kim, Michael R. Kreutz, Peter S. McPherson, Ben M. Neale, Vincent O'Connor, Danielle Posthuma, Timothy A. Ryan, Carlo Sala, Guoping Feng, Steven E. Hyman, Paul D. Thomas, August B. Smit, Matthijs Verhage
Article
Neurosciences
Ghazaleh Ashrafi, Jaime de Juan-Sanz, Ryan J. Farrell, Timothy A. Ryan
Editorial Material
Neurosciences
Ryan J. Farrell, Timothy A. Ryan
TRENDS IN NEUROSCIENCES
(2020)
Article
Multidisciplinary Sciences
Camila Pulido, Timothy A. Ryan
Summary: The brain is metabolically fragile, especially in terms of fuel availability impacting cognitive function. Synaptic vesicle pools are a major source of presynaptic energy consumption, contributing significantly to nerve terminal tolerance to fuel deprivation. This basal metabolic process arises from compensatory mechanisms within synaptic vesicles.
Article
Biology
Daniel C. Cook, Timothy A. Ryan
Summary: Control of neurotransmission efficacy is crucial for understanding brain's information processing. Presynaptic GPCRs, which can locally affect synaptic strength, have been found to inhibit voltage-gated calcium influx, contributing to complete silencing of nerve terminals. These findings suggest that neural circuits can modulate information throughput at the single synapse level by leveraging the non-linear relationship between action potential driven calcium influx and external calcium concentration.
Article
Neurosciences
Christina L. Torturo, Zhen-Yu Zhou, Timothy A. Ryan, Hugh C. Hemmings
Article
Biochemistry & Molecular Biology
Ying Wang, Manikandan Subramanian, Arif Yurdagul, Valeria C. Barbosa-Lorenzi, Bishuang Cai, Jaime de Juan-Sanz, Timothy A. Ryan, Masatoshi Nomura, Frederick R. Maxfield, Ira Tabas