Article
Virology
Clayton J. Wollner, Michelle Richner, Mariah A. Hassert, Amelia K. Pinto, James D. Brien, Justin M. Richner
Summary: Dengue virus is a common vector-borne viral disease with nearly 400 million infections worldwide annually. Vaccination with a novel mRNA vaccine encoding DENV1 structural proteins induced neutralizing antibody and cellular immune responses, effectively protecting against a lethal DENV challenge. The vaccine provided serotype-specific immunity and reduced antibody-dependent enhancement compared to live viral infection, highlighting its potential as an effective dengue vaccine.
JOURNAL OF VIROLOGY
(2021)
Article
Immunology
Li Hao, Michelle M. Kuttel, Neil Ravenscroft, Allison Thompson, A. Krishna Prasad, Seema Gangolli, Charles Tan, David Cooper, Wendy Watson, Paul Liberator, Michael W. Pride, Kathrin U. Jansen, Annaliesa S. Anderson, Ingrid L. Scully
Summary: This study investigated the protection mechanism of pneumococcal polysaccharide conjugate vaccines against different serotypes, showing that the 15B conjugate in a 20-valent PCV induced cross-functional antibody responses against 15C but not 15A. A rapid differentiation of closely related serotypes such as 15B from 15C and 15A was achieved using whole-genome sequencing data and molecular dynamics simulations.
Article
Immunology
Li Hao, Michelle M. Kuttel, Neil Ravenscroft, Allison Thompson, A. Krishna Prasad, Seema Gangolli, Charles Tan, David Cooper, Wendy Watson, Paul Liberator, Michael W. Pride, Kathrin U. Jansen, Annaliesa S. Anderson, Ingrid L. Scully
Summary: The study examined the protective effect of antibodies induced by pneumococcal polysaccharide conjugate vaccines against different serotypes, as well as the distinction between serotypes like 15B and 15C, demonstrating cross-functional antibody responses of PCV20 against serotypes 15B and 15C.
Review
Immunology
Irina Isakova-Sivak, Ekaterina Stepanova, Daria Mezhenskaya, Victoria Matyushenko, Polina Prokopenko, Ivan Sychev, Pei-Fong Wong, Larisa Rudenko
Summary: Current research has focused on improving antibody responses to conserved B cell epitopes and inducing T cells recognizing highly conserved viral epitopes for universal influenza vaccines. The development of T cell-based universal influenza vaccines is challenging due to factors such as the diversity of MHCs in the population and the lack of adequate animal models. Vaccines that elicit significant levels of T cells recognizing conserved epitopes possess high cross-protective potential against circulating influenza viruses.
EXPERT REVIEW OF VACCINES
(2021)
Article
Immunology
Xiaoyan Huang, Yaohui Li, Ruihua Li, Shaoyan Wang, Lu Yang, Shuyi Wang, Ying Yin, Xiaodong Zai, Jun Zhang, Junjie Xu
Summary: The study demonstrates that recombinant vaccine candidates using replication-defective adenovirus vector to deliver NiV attachment glycoprotein can effectively induce specific humoral and cellular immune responses in mice. The Ad5-NiVGe vaccine candidate elicited the strongest immune responses after a single-dose immunization. Additionally, a potent conserved T-cell epitope shared by NiV and HeV was identified, providing valuable information on HNVs-specific cellular immunity mechanism.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Zhe Chen, Chaoyang Meng, Junhua Mai, Yongbin Liu, Hangwen Li, Haifa Shen
Summary: This study demonstrates that a lipid-formulated RNA vaccine composed of a protamine/mRNA core and a lipid shell is highly effective in promoting cytotoxic CD8 T cell responses and mediating anti-tumor immunity. Both the mRNA core and lipid shell are necessary for stimulating the expression of type I interferons and inflammatory cytokines in dendritic cells. The expression of interferon relies exclusively on STING, and the antitumor activity of the mRNA vaccine is compromised in mice with a defective Sting gene. Thus, the mRNA vaccine induces STING-dependent antitumor immunity.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Immunology
Selma Belhimeur, Sylvie Briquet, Roger Peronet, Jennifer Pham, Pierre-Henri Commere, Pauline Formaglio, Rogerio Amino, Artur Scherf, Olivier Silvie, Salaheddine Mecheri
Summary: Plasmodium sporozoites (SPZ) migrate to the liver before infecting hepatocytes. IL-6 production in the liver has been shown to be detrimental for the parasite growth and contributes to long-lasting immune protection. This study explores how the parasite itself encodes for murine IL-6 and its effects on parasite virulence.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Marisa E. Aikins, You Qin, Hannah E. Dobson, Alireza Hassani Najafabadi, Kexing Lyu, Yao Xu, Ying Xin, Anna Schwendeman, Max S. Wicha, Alfred E. Chang, Qiao Li, James J. Moon
Summary: One major obstacle in cancer treatment is the presence of cancer stem cells (CSCs), which are resistant to traditional therapies and contribute to metastasis and relapse. In this study, a therapeutic vaccine targeting CSCs in melanoma was developed and shown to effectively reduce tumor growth and improve survival. The vaccine stimulated strong T cell responses against CSC-associated antigens and promoted infiltration of CD8+ T cells into tumors, while reducing the frequency of CSCs. This approach represents a safe and cost-effective strategy for targeting and eliminating CSCs in melanoma and other types of cancer.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Biotechnology & Applied Microbiology
Na Young Kim, Won Rak Son, Min Hoon Lee, Hong Seok Choi, Jun Young Choi, Young Jo Song, Chi Ho Yu, Dong Hyun Song, Gyeung Haeng Hur, Seong Tae Jeong, Sung Youl Hong, Young Kee Shin, Sungho Shin
Summary: This study developed a synthetic dual-pathogen DNA vaccine for anthrax and botulism, which elicited high levels of antibody response and provided over 50% protection against both pathogens. The study suggests a prospective approach for developing emergency vaccines that can be distributed on a massive scale in response to biothreat emergencies or infectious disease outbreaks.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Immunology
Jenny E. Hernandez-Davies, Jiin Felgner, Shirin Strohmeier, Egest James Pone, Aarti Jain, Sharon Jan, Rie Nakajima, Algimantas Jasinskas, Erwin Strahsburger, Florian Krammer, Philip L. Felgner, D. Huw Davies
Summary: Combining variant antigens into a multivalent vaccine is a traditional approach to provide broad coverage against antigenically variable pathogens. The use of a potent combination adjuvant can enhance the breadth of antibody response to different HA subtypes, potentially future-proofing vaccines against newly-emerging variants.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Li Chen, Haiwei Zhang, Moxuan Li, Bihao Wu, Zhe Zhang, Rui Gong
Summary: In this study, researchers developed an intranasal vaccine using a fusion of SARS-CoV-2 RBD and antibody Fc fragment. This vaccine could induce strong humoral immune responses and establish mucosal immunity in the respiratory tract. It also showed effectiveness in neutralizing various SARS-CoV-2 variants, including the Omicron variant. The findings suggest that this mucosal vaccine has the potential to reduce the risk of SARS-CoV-2 infection.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Lionel Sacconnay, Jonathan De Smedt, Vera Rocha-Perugini, Edison Ong, Romuald Mascolo, Anne Atas, Carline Vanden Abeele, Magali de Heusch, Nathalie De Schrevel, Marie-Pierre David, Badiaa Bouzya, Kim Stobbelaar, Yannick Vanloubbeeck, Peter L. Delputte, Corey P. Mallett, Nancy Dezutter, Lucile Warter
Summary: Aneuploidy, or chromosome copy number changes, is common in human cancers, but its role in cancer depends on the expression of individual oncogenes. Certain aneuploidies, such as the gain of chromosome 1q, may block p53 signaling. This study demonstrates that aneuploidy increases gene expression.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Microbiology
Eun Kim, Muhammad S. Khan, Alessandro Ferrari, Shaohua Huang, Jose C. Sammartino, Elena Percivalle, Thomas W. Kenniston, Irene Cassaniti, Fausto Baldanti, Andrea Gambotto
Summary: The study evaluates the booster effect of an S1 subunit vaccine in aged mice previously primed with adenoviral vaccines. It demonstrates that a booster dose of the rS1Beta subunit vaccine, given to mice, induces strong and long-lived S1-specific immune responses and significantly increases neutralizing antibodies against Wuhan, Beta, and Delta strains, including cross-reactive antibodies against Omicron variants. The findings suggest that the rS1Beta subunit vaccine can offer cross-neutralization against broad variants, providing important implications for controlling breakthrough SARS-CoV-2 variants in elderly individuals primed with adenovirus-based vaccines.
MICROBIOLOGY SPECTRUM
(2023)
Article
Medicine, General & Internal
Shanna S. Leventhal, Kimberly Meade-White, Deepashri Rao, Elaine Haddock, Jacqueline Leung, Dana Scott, Jacob Archer, Samantha Randall, Jesse H. Erasmus, Heinz Feldmann, David W. Hawman
Summary: This study evaluated an alphavirus-based replicon RNA vaccine expressing either the CCHFV nucleoprotein or glycoprotein precursor in a stringent, heterologous lethal challenge mouse model. It was found that vaccination with the RNA expressing the nucleoprotein alone provided complete protection against clinical disease, while vaccination with a combination of both the nucleoprotein and glycoprotein precursor offered robust protection against disease and viral replication. Unexpectedly, analysis of the immune responses elicited by the vaccine components showed that vaccination resulted in antibodies against the internal viral nucleoprotein and cellular immunity against the virion-exposed glycoproteins.
Article
Biotechnology & Applied Microbiology
Geetanjali Pendyala, J. Mauricio Calvo-Calle, Alberto Moreno, Ravi S. Kane
Summary: A vaccine based on SpyCatcher-mi3 nanoparticles presenting a chimeric PfCSP antigen was designed to target the malaria parasite Plasmodium falciparum (Pf). This vaccine incorporates recently identified T1/junctional epitopes and reduces the number of (NANP)(n) repeats. Immunization with this vaccine in mice resulted in high and durable IgG antibody levels and a balanced antibody response against T1/junctional epitopes and (NANP)(n) repeats. Refocusing the immune response towards functionally relevant epitopes may lead to a more effective second generation PfCSP-based vaccine.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Parasitology
Mariama K. Cherif, Guillaume S. Sanou, Edith C. Bougouma, Amidou Diarra, Alphonse Ouedraogo, Amagana Dolo, Marita Troye-Blomberg, David R. Cavanagh, Michael Theisen, David Modiano, Sodiomon B. Sirima, Issa Nebie
Article
Immunology
Karen M. Walker, Shinji Okitsu, David W. Porter, Christopher Duncan, Mario Amacker, Gerd Pluschke, David R. Cavanagh, Adrian V. S. Hill, Stephen M. Todryk
Article
Oncology
Graeme Cowan, Nicola J. Weston-Bell, Dean Bryant, Anja Seckinger, Dirk Hose, Niklas Zojer, Surinder S. Sahota
Article
Microbiology
Liz Stevenson, Erik Laursen, Graeme J. Cowan, Betty Bandoh, Lea Barfod, David R. Cavanagh, Gregers R. Andersen, Lars Hviid
Article
Immunology
Shatakshi Sood, Rebecca J. Brownlie, Celine Garcia, Graeme Cowan, Robert J. Salmond, Shimon Sakaguchi, Rose Zamoyska
JOURNAL OF IMMUNOLOGY
(2016)
Article
Infectious Diseases
Seth A. Amanfo, Takafira Mduluza, Nicholas Midzi, David R. Cavanagh, Francisca Mutapi
Article
Infectious Diseases
Bright Adu, Mariama K. Cherif, Samuel Bosomprah, Amidou Diarra, Fareed K. N. Arthur, Emmanuel K. Dickson, Giampietro Corradin, David R. Cavanagh, Michael Theisen, Sodiomon B. Sirima, Issa Nebie, Daniel Dodoo
Article
Infectious Diseases
Donna Bryan, Nilupa Silva, Peter Rigsby, Thomas Dougall, Patrick Corran, Paul W. Bowyer, Mei Mei Ho
Article
Infectious Diseases
Itziar Ubillos, Alfons Jimenez, Marta Vidal, Paul W. Bowyer, Deepak Gaur, Sheetij Dutta, Benoit Gamain, Ross Coppel, Virander Chauhan, David Lanar, Chetan Chitnis, Evelina Angov, James Beeson, David Cavanagh, Joseph J. Campo, Ruth Aguilar, Carlota Dobano
Article
Immunology
Katherine Miles, Joanne Simpson, Sheila Brown, Graeme Cowan, David Gray, Mohini Gray
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Immunology
Graeme J. M. Cowan, Katherine Miles, Lorenzo Capitani, Sophie S. B. Giguere, Hanna Johnsson, Carl Goodyear, Iain B. McInnes, Steffen Breusch, David Gray, Mohini Gray
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Immunology
Natasha L. Smith, Wiebke Nahrendorf, Catherine Sutherland, Jason P. Mooney, Joanne Thompson, Philip J. Spence, Graeme J. M. Cowan
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Immunology
Elinor Wing, Catherine Sutherland, Katherine Miles, David Gray, Carl S. Goodyear, Thomas D. Otto, Stefan Breusch, Graeme Cowan, Mohini Gray
Summary: This study reveals the relationship between different subsets of B cells in the synovium of rheumatoid arthritis and pathogenic antibody secreting cells (ASCs), and identifies DN2 B cells as major precursors to synovial ASCs. This knowledge is crucial for developing more targeted therapies for B-cell depletion in rheumatoid arthritis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemical Research Methods
Catherine Sutherland, Graeme J. M. Cowan
Summary: Adaptive Immune Receptor Repertoire Sequencing is a rapidly developing field that has improved our understanding of the adaptive immune system. We have developed AIRRSHIP, a Python package that produces synthetic human B cell receptor sequences, which can be used to assess the accuracy of repertoire analysis tools and identify factors contributing to inaccuracies in results.
Meeting Abstract
Public, Environmental & Occupational Health
Liz Stevenson, Erik Laursen, Graeme J. Cowan, Betty Bandoh, Lea Barfod, David R. Cavanagh, Gregers R. Andersen, Lars Hviid
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2015)