Article
Infectious Diseases
Sang-Guen Kim, Sung-Bin Lee, Su-Jin Jo, Kevin Cho, Jung-Kum Park, Jun Kwon, Sib Sankar Giri, Sang-Wha Kim, Jeong-Woo Kang, Won-Joon Jung, Young-Min Lee, Eunjung Roh, Se-Chang Park
Summary: This study suggests the potential of using a combination of bacteriophages and antibiotics as an effective strategy to control the outbreak of blight disease. The combination showed improved host infectivity coverage and achieved synergistic effects in inhibiting bacterial growth. The phage cocktail demonstrated higher antibacterial activity compared to the antibiotic alone.
Article
Horticulture
Gulshariya Kairova, Nurzhan Daulet, Maxim Solomadin, Nurlan Sandybayev, Shynggys Orkara, Vyacheslav Beloussov, Nazym Kerimbek, Dilyara Gritsenko, Zagipa Sapakhova
Summary: This study conducted phytopathological evaluation and molecular genetic analysis of 59 apple varieties in different regions of Kazakhstan, finding that 26 varieties showed the highest resistance to fire blight. Interestingly, all 59 apple cultivars had identical genotypes for the 10 markers associated with fire blight resistance.
Article
Infectious Diseases
Annette Wimmer, Michael Glueck, Wenzi Ckurshumova, Jun Liu, Michael Fefer, Kristjan Plaetzer
Summary: This study aims to demonstrate that photodynamic inactivation can be used to treat plant diseases even if the causative bacteria are resistant to conventional treatment. Different photosensitizers in combination with different culture conditions have varying effects on bacterial inactivation, and PDI based on natural photosensitizers might represent an eco-friendly treatment strategy.
Review
Plant Sciences
Quan Zeng, Joanna Pulawska, Jeffrey Schachterle
Summary: This review examines the different infection stages of the fire blight disease caused by Erwinia amylovora, focusing on the early events during the initial infection of flowers and shoot tips. It discusses the production and transfer of inoculum, epiphytic proliferation of the pathogen, entry into the host, and initial proliferation inside the host. Additionally, the review delves into how E. amylovora modulates its virulence expression to induce disease symptoms during these early events.
JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Entomology
Matthew Boucher, Rowan Collins, Stephen Hesler, Kerik Cox, Greg Loeb
Summary: The study found that Delia platura flies prefer infected and oozing fruit over uninfected fruit, and this preference diminishes when bacterial ooze is removed. However, the flies do not show a preference between infected saplings and uninfected saplings. Additionally, the volatiles from infected fruit do not attract the flies, indicating that diseased fruit odor is not the reason for their preference.
ENVIRONMENTAL ENTOMOLOGY
(2021)
Article
Plant Sciences
Ju Yeon Song, Yeo Hong Yun, Gi-Don Kim, Seong Hwan Kim, Seong-Jin Lee, Jihyun F. Kim
Summary: The emergence of fire blight caused by Erwinia amylovora in Korea represents a significant development in the understanding and potential control of this highly contagious disease. Genomic analysis of the strains isolated from Korean infected trees revealed their close relationship to North American strains, providing valuable insights for further research and development of control strategies.
Article
Biochemistry & Molecular Biology
Xiaochen Yuan, Gayle C. McGhee, Suzanne M. Slack, George W. Sundin
Summary: Erwinia amylovora requires plasmid pEA29 to produce amylovoran and form a biofilm, with regulation depending on the thiamine biosynthesis operon thiOSGF. Thiamine-mediated effect on amylovoran production is indirect, and exogenous thiamine can restore and enhance amylovoran production.Overall, a novel signaling pathway involving thiamine biosynthesis enhances bacterial respiration for EPS amylovoran biosynthesis, impacting E. amylovora virulence.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Agriculture, Multidisciplinary
Duck Kyu Ryu, Mahesh Adhikari, Dong Hyuk Choi, Kyung Jin Jun, Do Hyoung Kim, Chae Ryeong Kim, Min Kyu Kang, Duck Hwan Park
Summary: Fire blight, caused by Erwinia amylovora, is a major bacterial disease in apple and pear trees, resulting in significant economic losses worldwide. This study analyzed the mechanisms and disease severity reduction efficacy of copper-based control agents (CBCAs) against E. amylovora. The results showed that copper oxychloride, copper sulfate basic, and tribasic copper sulfate at 2,000 ppm had significant reduction effects on fire blight.
PLANT PATHOLOGY JOURNAL
(2023)
Article
Agriculture, Multidisciplinary
Yeong Seok Kim, Men Thi Ngo, Bomin Kim, Jae Woo Han, Jaekyeong Song, Myung Soo Park, Gyung Ja Choi, Hun Kim
Summary: In this study, a fungal isolate with antibacterial activity against E. amylovora was identified and characterized. The active compound from the culture was found to effectively suppress the development of fire blight disease. This research provides new insights into the potential of using P. brasilianum for biocontrol of fire blight.
PLANT PATHOLOGY JOURNAL
(2022)
Article
Plant Sciences
Gaucher Matthieu, Righetti Laura, Aubourg Sebastien, Duge de Bernonville Thomas, Brisset Marie-Noelle, Chevreau Elisabeth, Vergne Emilie
Summary: The pPPO16 promoter, cloned from apple, is a useful tool for creating fire blight resistant apple genotypes without using transgenic methods. It allows fast and strong expression of transgenes in apple, making it a potential regulator.
PLANT CELL REPORTS
(2022)
Article
Plant Sciences
S. C. Razo, I. Safenkova, N. Drenova, A. A. Kharchenko, Y. S. Tsymbal, Y. A. Varitsev, A. Zherdev, E. N. Pakina, B. B. Dzantiev
Summary: The study developed a lateral flow immunoassay (LFIA) tool to detect Erwinia amylovora that causes fire blight, and tested its effectiveness on different plant organs and species. The results showed that LFIA had high specificity and detection sensitivity, and its effectiveness significantly improved when more appropriate samples were used.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2021)
Article
Biology
Jaein Choe, Byeori Kim, Mi-Kyung Park, Eunjung Roh
Summary: In this study, Phi Fifi106, isolated from pear orchard soil, was purified and characterized, and its efficacy for controlling fire blight in apple plants was evaluated. The results showed that Phi Fifi106 efficiently inhibited the infection of E. amylovora and reduced the disease incidence and severity in M9 apple plants.
Article
Plant Sciences
Sara M. Klee, Judith P. Sinn, Jeremy Held, Chad Vosburg, Aleah C. Holmes, Brian L. Lehman, Kari A. Peter, Timothy W. McNellis
Summary: The transcription antiterminator RfaH plays a crucial role in the full virulence of Erwinia amylovora by reducing pausing in the synthesis of key virulence factors. RfaH specifically suppresses operon polarity in the synthesis of amylovoran and lipopolysaccharides, which are essential for the pathogenicity of E. amylovora.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhouqi Cui, Regan B. Huntley, Neil P. Schultes, Kaleem U. Kakar, Ching-Hong Yang, Quan Zeng
Summary: The expression of the type III secretion system (T3SS) in epiphytic Erwinia amylovora cells on stigma and hypanthium of apple flowers varies, with higher expression and promotion of epiphytic growth on stigma surfaces compared to hypanthium surfaces. Pre-culturing E. amylovora cells on stigma enhances T3SS expression and potential for infection, suggesting that T3SS induction during stigmatic colonization may benefit subsequent infection. The expression of T3SS is also influenced by relative humidity, with a higher percentage of T3SS expressing cells under high humidity conditions.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Review
Plant Sciences
Roshni R. Kharadi, George W. Sundin
Summary: Erwinia amylovora, the causal agent of fire blight, infects hosts through flowers or young leaves, using a Type III secretion system to establish bacterial populations, invade xylem, form biofilms, and eventually cause wilting symptoms. Attachment and biofilm formation in xylem are regulated by small RNAs, mechanical forces, and the second messenger molecule c-di-GMP.
JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Agronomy
Hubert Glos, Hanna Bryk, Monika Michalecka, Joanna Pulawska
Summary: The study found that the occurrence of storage diseases on apples grown in the central part of Poland varied depending on the season, apple cultivar, and orchard location. Bull's eye rot and gray mold were the most common diseases observed on different apple cultivars, while blue mold, brown rot, and Alternaria spp. occurred at lower intensities. New storage diseases caused by Colletotrichum spp., Neonectria ditissima, and Diaporthe eres were also detected during the study period.
Article
Biotechnology & Applied Microbiology
Angel Oliveras, Cristina Camo, Pau Caravaca-Fuentes, Luis Moll, Gerard Riesco-Llach, Sergio Gil-Caballero, Esther Badosa, Anna Bonaterra, Emilio Montesinos, Lidia Feliu, Marta Planas
Summary: Thirty peptide conjugates were designed to show high antibacterial activity against plant-pathogenic bacteria, with the best peptides incorporating Pep13. The conjugation order and structural parameters were found to influence the biological profile of the peptides.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Infectious Diseases
Theo H. M. Smits, Lavinia N. V. S. Arend, Sofia Cardew, Erika Tang-Hallback, Marcelo T. Mira, Edward R. B. Moore, Jorge L. M. Sampaio, Fabio Rezzonico, Marcelo Pillonetto
Summary: With improvements in genomics, it is now possible to accurately identify clinical isolates at the species level and analyze past occurrences of specific organisms. This approach can aid in the classification of clinically relevant taxa that have previously gone unrecognized in clinical diagnostics.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
Joanna Pulawska, Nemanja Kuzmanovic, Pawel Trzcinski
Summary: Four non-pathogenic strains isolated from galls on blueberry plants were characterized and found to represent a novel species of the genus Agrobacterium named Agrobacterium vaccinii sp. nov.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2022)
Article
Environmental Sciences
Silvia Barbe, Angela Figas-Segura, M'hamed Benada, Inmaculada Navarro-Herrero, Telma Maria Sampaio, Elena G. Biosca, Ester Marco-Noales
Summary: This study identified bacterial strains with antagonistic activity against Erwinia amylovora, the causative agent of fire blight, and demonstrated their biocontrol potential. These strains showed no hypersensitivity reaction in tobacco leaves, produced hydrolytic enzymes, and exhibited other biocontrol and plant growth-promoting activities.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Nemanja Kuzmanovic, Enrico Biondi, Joerg Overmann, Joanna Pulawska, Susanne Verbarg, Kornelia Smalla, Florent Lassalle
Summary: Comparative genomics and phylogenomic analysis provide new insights into the diversification and taxonomy of the Allorhizobium vitis species complex, leading to the redefinition of A. vitis sensu stricto and the description of A. ampelinum. Pan-genome analyses reveal that these species have distinct ecologies, relying on specialized nutrient consumption or toxic compound degradation to adapt to their niche. Additionally, specific genes identified in the different species within the complex show potential roles in ecological diversification and stress tolerance, which contribute to the differentiation seen in the pan-genome analysis.
Article
Plant Sciences
Aina Baro, Pasquale Saldarelli, Maria Saponari, Emilio Montesinos, Laura Montesinos
Summary: This study evaluated the control of Xylella fastidiosa infections in Nicotiana benthamiana using the transient expression of BP134 and BP178 genes and synthetic peptide endotherapy. Transient production of BP178 significantly decreased disease severity, and plants treated with synthetic BP134 and BP178 showed consistently lower levels of disease. However, coinfection with PVX-BP134 and X. fastidiosa caused plant death. The levels of X. fastidiosa significantly decreased after BP178 treatment, but not after transient production. Both treatments induced the expression of defense-related genes. The protection observed with transiently produced BP178 seems mainly mediated by the induction of plant defense.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Emilio Montesinos
Summary: Plant disease control requires new approaches to combat current, emerging, and re-emerging diseases and adapt to climate change and pesticide restrictions. Functional peptides offer novel mechanisms to fight plant pathogens but face challenges in stability, formulation, toxicology, and cost.
ANNUAL REVIEW OF PHYTOPATHOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Michal Warabieda, Nemanja Kuzmanovic, Pawel Trzcinski, Joanna Pulawska
Summary: Three plant rhizogenic strains O132T, O115 and O34 isolated from Cucumis sp. L. are classified as a novel species, Agrobacterium cucumeris, based on their genetic sequences, DNA-DNA hybridization, and physiological characteristics compared with their closest relatives in the genus Agrobacterium. These strains belong to the separate sub-clade biovar 1 within the genus Agrobacterium.
SYSTEMATIC AND APPLIED MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Maria Quintana-Gonzalez de Chaves, Felix Moran, Silvia Barbe, Edson Bertolini, Felipe Siverio de la Rosa, Ester Marco-Noales
Summary: A new quantitative real-time PCR protocol has been developed to detect four known phytopathogenic species of the genus Liberibacter, which shows higher specificity and sensitivity compared to previous protocols. It can be used as a rapid and time-saving screening test for simultaneous detection of all plant pathogenic species of 'Ca. Liberibacter' in a one-step assay.
SCIENTIFIC REPORTS
(2023)
Article
Plant Sciences
Laura Montesinos, Aina Baro, Beatriz Gascon, Emilio Montesinos
Summary: The activity of Eucalyptus essential oil against plant pathogenic bacteria was studied and it was found that the formulation EGL2 was effective in inhibiting the growth of various strains. It also exhibited a strong bactericidal effect, causing significant reductions in bacterial survival. Additionally, the preventive spray application of EGL2 on pear plants reduced the severity of Erwinia amylovora infections.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Agata Broniarek-Niemiec, Jorunn Borve, Joanna Pulawska
Summary: Bacterial canker, caused by Pseudomonas syringae pv. syringae and pv. morsprunorum, is a significant disease affecting stone fruit trees. Research conducted in central Poland from 2018 to 2021 evaluated the efficacy of different chemical and biological products in controlling the disease. The study found that products containing copper compounds and copper sulfate were highly effective against bacterial canker.
Editorial Material
Plant Sciences
Artur Mikicinski, Michal Warabieda, Jacek S. Nowak, Joanna Pulawska
JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Agronomy
Anna Poniatowska, Monika Michalecka, Joanna Pulawska
Summary: This study developed a quick and sensitive method for detecting asymptomatic brown rot fungi in apples. The LAMP reaction and DNA preamplification were employed to improve the detection sensitivity, and the method was validated using infected apple fruit samples with and without visible brown rot symptoms.
Article
Agronomy
Nay C. Dia, Bart Cottyn, Jochen Blom, Theo H. M. Smits, Joel F. Pothier
Summary: This study developed loop-mediated isothermal amplification (LAMP) diagnostic assays for rapid and accurate detection of the X. hortorum - X. hydrangeae species complex. Genomic analysis was used to identify genomic targets for the assays. The assays showed 100% sensitivity, specificity, and efficiency on a set of reference strains and a validation set.
FRONTIERS IN AGRONOMY
(2022)