Article
Biochemistry & Molecular Biology
Won Jin Lee, Hyuk Gyoon Lee, Jinwoo Hur, Gyeong Hee Lee, Jun Pil Won, Eunsu Kim, Jung Seok Hwang, Han Geuk Seo
Summary: The nuclear receptor PPAR delta plays a regulatory role in cellular iron homeostasis, and its activation can alleviate 6-OHDA-induced neurotoxicity. PPAR delta activation reduces iron accumulation and suppresses the generation of reactive oxygen species and lipid peroxides.
Article
Medicine, Research & Experimental
Shalini Singh, Sourav Ghosh, Virender Kumar Pal, MohamedHusen Munshi, Pooja Shekar, Diwakar Tumkur Narasimha Murthy, Govindasamy Mugesh, Amit Singh
Summary: V2O5 nanosheets mimic natural glutathione peroxidase activity to mitigate ROS in HIV-1 infected cells, blocking viral reactivation and replication. This suggests nanozymes as potential platforms for developing interventions against infectious diseases, highlighting the therapeutic potential of V2O5 nanosheets against HIV.
EMBO MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Bingrui Wang, Jichang Li, Junzhe Jiao, Min Xu, Yichun Luo, Fang Wang, Qiang Xia, Yueqiu Gao, Yu Feng, Xiaoni Kong, Xuehua Sun
Summary: The study demonstrates that deletion of DJ-1 protects against APAP-induced acute liver injury by reducing inflammatory response, potentially through modulating myeloid cell function.
Article
Biochemistry & Molecular Biology
James N. N. Tsoporis, Hajera Amatullah, Sahil Gupta, Shehla Izhar, Amin M. M. Ektesabi, Chirag M. M. Vaswani, Jean-Francois Desjardins, Golam Kabir, Ana Paula Teixera Monteiro, Amir K. K. Varkouhi, Nikolaos Kavantzas, Vasileios Salpeas, Ioannis Rizos, John C. C. Marshall, Thomas G. G. Parker, Howard Leong-Poi, Claudia C. C. dos Santos
Summary: Oxidative stress is an early contributor to sepsis-induced myocardial depression, and DJ-1 plays a role as an antioxidant. DJ-1 deficiency improves survival and bacterial clearance in sepsis. This study investigates the role of DJ-1 in sepsis-induced myocardial depression and suggests DJ-1 as a potential therapeutic target.
Article
Neurosciences
Yuan Ge, Xinlei Zheng, Shiyu Mao, Qingyu Zhang, Gang Hu, Yao Wei
Summary: Our study demonstrates that the loss of DJ-1 in astrocytes promotes the degradation of glutathione (GSH), making neurons more vulnerable to oxidative damage. This finding provides a theoretical basis for the development of drugs targeting DJ-1 and GSH in the brain.
NEUROSCIENCE RESEARCH
(2022)
Article
Environmental Sciences
Camila G. Dantas, Ailma O. da Paixao, Tassia L. G. M. Nunes, Italo J. F. Silva, Bruno dos S. Lima, Adriano A. S. Araujo, Ricardo L. C. de Albuquerque-Junior, Katia P. Gramacho, Francine F. Padilha, Luiz P. da Costa, Patricia Severino, Juliana C. Cardoso, Eliana B. Souto, Margarete Z. Gomes
Summary: This study evaluated the neuroprotective effects of Africanized bee venom (BV) on a mice model of Parkinson's disease induced by 6-hydroxydopamine (6-OHDA). The results showed that BV could counteract the symptoms caused by 6-OHDA, reduce cell death, and protect the striatum from damage. These findings suggest that Africanized bee venom may have potential as a novel therapeutic approach for Parkinson's disease.
Article
Biochemistry & Molecular Biology
Okina Sakakibara, Mikako Shimoda, Gaku Yamamoto, Youichirou Higashi, Mayumi Ikeda-Imafuku, Yu Ishima, Masahiro Kawahara, Ken-ichiro Tanaka
Summary: Parkinson's disease (PD) is a neurodegenerative disorder characterized by loss of dopaminergic neurons and elevated inflammatory responses. The fusion protein Alb-Trx has shown effectiveness against respiratory and renal diseases. This study found that Alb-Trx inhibited neurotoxicity and oxidative stress in PD, improving intracellular signaling pathways and suppressing neuroinflammation. This suggests Alb-Trx may be a potential therapeutic agent for PD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Margarida Neves, Mario Graos, Sandra Anjo, Bruno Manadas
Summary: Efforts have been made to understand the physiological and pathological role of DJ-1, a Parkinson's disease (PD) associated protein, to provide new insights into PD pathophysiology. DJ-1 has been found to play several neuroprotective roles, with its ability to modulate signaling pathways being of utmost importance for cell death regulation. The most relevant findings regarding DJ-1's regulatory activity on signaling cascades, including ERK1/2, PI3K/Akt, ASK1, Nrf2, and p53 pathways, have been presented.
Article
Multidisciplinary Sciences
Xinyu Zhou, Nanqu Huang, Xiaoyi Hou, Li Zhu, Yiman Xie, Zhisheng Ba, Yong Luo
Summary: The study found that ICT can protect against cell damage induced by 6-OHDA by attenuating oxidative stress. ICT can increase SOD activity and TH expression, reduce ROS production and a-Syn expression, and promote the translocation of Nrf2 from the cytoplasm to the nucleus and increase the protein expression of HO-1.
Article
Biochemistry & Molecular Biology
Hiroki Yamamoto, Takuya Fujiwara, Takashi Funatsu, Makoto Tsunoda
Summary: This study developed a method for intracellular thiol quantification using HPLC-fluorescence detection. The method can separate six different SBD-thiols within 30 minutes and was used to quantify thiol concentrations in chronic myelogenous leukemia K562 cell samples, as well as the time-dependent effect of a thiol scavenger, N-ethyl maleimide, on intracellular thiol concentrations. This method is useful for elucidating the role of intracellular sulfur metabolism.
Article
Cell Biology
Ting-Yu Chin, Che-Chuan Wang, Kuo-Hsing Ma, Chia-Wei Kuo, Ming-Kuan Hu, Sheau-Huei Chueh
Summary: The study showed that DPMQ protects NG108-15 cells against oxidative stress by stabilizing DJ-1 in a Cu2+-dependent manner, leading to SOD1 activation and Nrf2 nuclear translocation, which alleviates cellular oxidative stress.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Cell Biology
Yunash Maharjan, Raghbendra Kumar Dutta, Jinbae Son, Xiaofan Wei, Channy Park, Hyug Moo Kwon, Raekil Park
Summary: This study compares the effects of three autophagic inhibitor drugs on cholesterol transport and autophagy flux. The results show that U1866A and bafilomycin A induce lysosomal cholesterol accumulation and inhibit autophagy flux. Accumulated cholesterol does not affect lysosomal pH. The restoration of defective autophagosome-lysosome fusion can only restore autophagy flux when lysosomal acidification is not altered.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Nutrition & Dietetics
Hussein Herz, Yang Song, Yuanchao Ye, Liping Tian, Benjamin Linden, Marwa Abu El Haija, Yi Chu, Justin L. Grobe, Randall W. Lengeling, Mohamad Mokadem
Summary: Chronic use of NSAIDs can induce small intestinal ulcerations, which can affect intestinal GLP-1 production, hepatic insulin sensitivity, and hepatic glucose production.
Article
Chemistry, Multidisciplinary
Lijie Chen, Xingwu Jiang, Meng Lv, Xueli Wang, Peiran Zhao, Meng Zhang, Guanglei Lv, Jiyue Wu, Yanyan Liu, Yang Yang, Jinquan Chen, Wenbo Bu
Summary: This study introduces a strategy to overcome cellular adaptation in cancer cells by utilizing the cells' antioxidant defenses. By inducing cellular maladaptation through reductive damage, the study shows abnormal activation of antioxidant defenses and induction of apoptosis in cancer cells.
Article
Biochemistry & Molecular Biology
Yuening Luo, Siqi Zhou, Reiko Takeda, Kazuma Okazaki, Marie Sekita, Kazuichi Sakamoto
Summary: In this study, amber extract was found to protect neuronal cells from apoptosis in a Parkinson's disease model by decreasing ROS levels and promoting autophagy.
Review
Biochemistry & Molecular Biology
Yoshiro Saito
JOURNAL OF BIOCHEMISTRY
(2020)
Article
Pharmacology & Pharmacy
Takuya Imatoh, Kimie Sai, Yoshiro Saito
JOURNAL OF CLINICAL PHARMACOLOGY
(2020)
Article
Environmental Sciences
Naoki Endo, Takashi Toyama, Akira Naganuma, Yoshiro Saito, Gi-Wook Hwang
Meeting Abstract
Biochemistry & Molecular Biology
Yoshiro Saito
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Yoshiro Saito
Summary: Lipid peroxidation and its products play a significant role in physiological and pathophysiological conditions, with mechanisms including enzymatic oxidation, free radical-mediated oxidation, and nonradical-mediated oxidation. The products of lipid peroxidation can lead to cytotoxicity and novel biological activity, while the antioxidant system, particularly selenoproteins and vitamin E, plays a crucial role in preventing lipid peroxidation and ferroptosis.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Multidisciplinary Sciences
Takashi Toyama, Takayuki Hoshi, Takuya Noguchi, Yoshiro Saito, Atsushi Matsuzawa, Akira Naganuma, Gi-Wook Hwang
Summary: Methylmercury-induced neuronal cell death in the mouse brain may be associated with activation of the ASK1/p38 pathway by mitochondrial ROS, leading to TNF-alpha expression and microglia involvement.
SCIENTIFIC REPORTS
(2021)
Review
Nutrition & Dietetics
Yoshiro Saito
Summary: SELENOP is a selenium-containing protein primarily produced in the liver, playing a pivotal role in selenium metabolism and antioxidative defense. It can reduce phospholipid hydroperoxides and deliver selenium to other tissues, but decreased or excessive levels can cause various dysfunctions. This review focuses on SELENOP's selenium transport system, molecular mechanism, and its biological significance.
FRONTIERS IN NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Yoshiro Saito
Summary: Vitamin E is a potent lipid-soluble antioxidant, consisting of tocopherol and tocotrienol. They have different isoforms which exhibit similar radical scavenging abilities, but offer unique cytoprotective effects in cells. The review focuses on the actions of vitamin E against oxidative stress and its relation to cellular uptake and distribution, as well as its role in preventing lipid peroxidation and ferroptosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Cell Biology
Machiko Honda, Tatsuya Segawa, Kiyoshi Ishikawa, Masahiro Maeda, Yoshiro Saito, Shigeyuki Kon
Summary: Blood levels of nephronectin (Npnt), a protein critical for kidney development, are elevated in EAE mice. Treatment with anti-Npnt antibody inhibits EAE development. The interaction between Npnt and SeP regulates the Th17/Treg balance via the ROS level.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nanako Kitabayashi, Shohei Nakao, Yuichiro Mita, Kotoko Arisawa, Takayuki Hoshi, Kiyo-aki Ishii, Toshinari Takamura, Noriko Noguchi, Yoshiro Saito, Takashi Toyama
Summary: Selenoprotein P (SELENOP) is a selenium-containing protein that plays a significant role in pancreatic beta cell function by maintaining the levels of cellular selenoproteins. Knockdown of the Selenop gene resulted in decreased cell viability, insulin levels, and insulin secretion, which were recovered by the addition of selenium. These findings suggest that SELENOP expression is important for the function of pancreatic beta cells.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Review
Environmental Sciences
Marco Vinceti, Tommaso Filippini, Ewa Jablonska, Yoshiro Saito, Lauren A. Wise
Summary: Recent evidence suggests that previous research underestimated the adverse effects of selenium exposure at low levels and the potential drawbacks of maximizing selenoprotein expression. This calls for a reassessment of the recommended range of selenium exposure and dietary reference values.
ENVIRONMENTAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Teresa Urbano, Marco Vinceti, Jessica Mandrioli, Annalisa Chiari, Tommaso Filippini, Roberta Bedin, Manuela Tondelli, Cecilia Simonini, Giovanna Zamboni, Misaki Shimizu, Yoshiro Saito
Summary: This study found abnormalities in the concentrations of selenium-transporter protein selenoprotein P in the central nervous system and blood, which are associated with amyotrophic lateral sclerosis (ALS) and neurocognitive disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Takayuki Kaneko, Yuichiro Mita, Kanako Nozawa-Kumada, Masana Yazaki, Mieko Arisawa, Etsuo Niki, Noriko Noguchi, Yoshiro Saito
Summary: This study reveals the role of hydrogen sulfide, hydropersulfides, and hydropolysulfides in cell signaling and protection against oxidative stress. The mechanism of action involves both two-electron and one-electron redox reactions, with the former receiving more attention. The study also highlights the antioxidant effects of these compounds in inhibiting lipid peroxidation.
FREE RADICAL RESEARCH
(2022)
Review
Pharmacology & Pharmacy
Ryouhei Tsutsumi, Yoshiro Saito
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2020)
Review
Nutrition & Dietetics
Yoshiro Saito
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
(2020)