Article
Microbiology
Ana Gaspar-Cordeiro, Catarina Amaral, Vania Pobre, Wilson Antunes, Ana Petronilho, Paulo Paixao, Antonio P. Matos, Catarina Pimentel
Summary: The combination of copper and fluconazole has synergistic effects against resistance Candida glabrata. Copper affects the efflux of fluconazole and the biosynthesis of ergosterol, leading to the accumulation of toxic sterols. It also affects protein function in the plasma membrane, causing structural alterations in cells. The combination treatment downregulates gene expression and induces zinc depletion, which contributes to the observed synergistic effect.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mirelle Flores-Villegas, Corinna Rebnegger, Viktoria Kowarz, Roland Prielhofer, Diethard Mattanovich, Brigitte Gasser
Summary: The promoter of the high-affinity glucose transporter Gth1 (PGTH1) was improved through random and rational engineering, resulting in stronger induction in glucose-limitation and enabling methanol-free production processes. Single point mutations and key promoter segments were identified, leading to a library of novel promoter variants with significantly higher activity. The study also discovered nucleotide positions and corresponding TFBS that distinguish between glycerol- and glucose-mediated repression of the native promoter.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Jana Aupic, Fabio Lapenta, Ziga Strmsek, Estera Merljak, Tjasa Plaper, Roman Jerala
Summary: Researchers have developed coiled-coil dimers that can assemble diverse protein architectures through dynamic self-assembly and respond to metal ions, providing a versatile tool for designing functional polypeptide nanostructures.
Article
Biochemistry & Molecular Biology
Yusuke Sekine, Ryan Houston, Eva -Maria Eckl, Evelyn Fessler, Derek P. Narendra, Lucas T. Jae, Shiori Sekine
Summary: In this study, the researchers found that the activation of heme-regulated kinase HRI under iron-deficient conditions requires the involvement of mitochondrial protein DELE1. The import and stability of DELE1 in the mitochondria is regulated by iron availability. Importantly, when iron is chelated, DELE1 import is inhibited, leading to its stabilization on the mitochondrial surface and activation of the HRI-mediated integrated stress response. This study also revealed that the DELE1-HRI-ISR pathway plays a protective role in iron-demanding cell lineages.
Article
Biochemistry & Molecular Biology
Eric Huntzinger, Jordan Sinteff, Bastien Morlet, Bertrand Seraphin
Summary: Proteins with RNB domain are present across various species, and a new RNB protein, HELZ2, was identified in humans. HELZ2 protein in humans is produced from an unexpected non-canonical initiation codon and can function as an active ribonuclease despite a key residue substitution. The activity of HELZ2 as a ribonuclease is lost in cancer cells due to somatic mutations. HELZ2 also contains RNA helicase domains and zinc fingers, and its expression is induced by interferon treatment. It is involved in degrading structured RNAs through ATP-dependent displacement mediated by its RNA helicase domains and ribonucleolytic action.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Physics, Fluids & Plasmas
Shih-Min Chang, Chia Ying Yu, Yi-Fan Chen
Summary: This study investigates the mechanism of action of pH-responsive gemini surfactants in endosomal escape. It is found that the pH-responsive gemini surfactants assist endosomal escape by releasing excess molecules that disrupt endosomal and lysosomal membranes.
Article
Multidisciplinary Sciences
Benjamin J. E. Martin, Julie Brind'Amour, Anastasia Kuzmin, Kristoffer N. Jensen, Zhen Cheng Liu, Matthew Lorincz, LeAnn J. Howe
Summary: Histone acetylation, a common feature of transcription, is largely dependent on transcription due to the recruitment and activity of histone acetyltransferases by RNA polymerase II. Promoter-bound HATs are unable to acetylate histones without active transcription, indicating that HAT activity is regulated post-recruitment.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Hephzibah Cathryn R., George Priya Doss C.
Summary: In this study, the effect of mutations on P53 protein was investigated using molecular dynamics simulation. The results showed that the mutations led to loss of hydrogen bonds between DNA binding bases and caused significant movements of the zinc-binding domain.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Microbiology
Li Fan, Wenqing Zhang, Josephine Rybchuk, Yu Luo, Wei Xiao
Summary: DNA-damage tolerance is a mechanism that allows eukaryotes to bypass replication-blocking lesions and continue DNA synthesis. In this study, a mutant with a pol30-A171D mutation was found to rescue DNA-damage sensitivity in a srs2-dependent and PCNA sumoylation-independent manner, providing insights into the molecular mechanism of the interaction between PCNA and Srs2.
Article
Nanoscience & Nanotechnology
Shushan Zheng, Mengyu Chen, Kui Chen, Yongjian Wu, Jing Yu, Tongtong Jiang, Mingzai Wu
Summary: Significantly improving the performance of Zn-air batteries is a challenging task due to the limitations of thermodynamic equilibrium potential. This study presents a prototype of a multi-functional air electrode material, FeNi-S,N-HCS, for designing photoresponsive all-solid-state Zn-air batteries based on the photothermal effect. The FeNi-S,N-HCS electrode exhibits superior photothermal effect, allowing for improved power density, reduced charge voltage, and prolonged cycle lifetime under illumination.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Nanoscience & Nanotechnology
Iris Seitz, Heini Ijaes, Veikko Linko, Mauri A. Kostiainen
Summary: DNA nanostructures are susceptible to degradation, but coating them with proteins can protect and control their interaction with antigens through optical stimulation.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Christopher T. Coey, David J. Clark
Summary: Sequence-specific DNA-binding transcription factors, such as Gcn4, play a central role in gene regulation. Gcn4 has a strong binding affinity to an AP-1-like sequence and a weaker affinity to half-sites. However, only a fraction of the exact matches to this sequence are bound by Gcn4, even in vitro. The binding of Gcn4 is independent of chromatin structure and is determined by the presence of nucleosome-depleted regulatory regions.
Article
Biochemistry & Molecular Biology
Karina A. Baksh, Dmitry Pichugin, Robert Scott Prosser, Deborah B. Zamble
Summary: Nickel plays a crucial role in the survival of the pathogenic bacteria Helicobacter pylori in the human stomach by regulating nickel homeostasis through the transcription factor NikR. Nickel binding induces conformational changes in HpNikR, enabling it to bind specific DNA motifs and influence gene transcription. Zinc is unable to induce the same allosteric response as nickel, suggesting that the mechanism of nickel-activated DNA binding by HpNikR is driven by conformational selection.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Yi Chang, Tingting Liu, Ruijuan Qi, Shuting Chen, Yuming Guo, Lin Yang, Xiaoming Ma
Summary: Metal-free elementary nanomaterials, particularly hollow hierarchical metal-free substances, are considered as next-generation green materials for biomedical applications. This study proposes a cell-regulated strategy to construct hollow sulfur nanospheres with porous shell, using yeast cell-derived biomolecules as regulators and stabilizers. These synthesized nanospheres have excellent drug loading efficiency and sustained release due to their internal space and permeable shell.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2022)
Article
Genetics & Heredity
Mingxiang Zhang, Jaelyn Z. Current, Heather L. Chaney, Jianbo Yao
Summary: This study identified a novel DNA binding element of the maternal effect gene ZNFO and determined the key nucleotides required for specific binding. The interaction between ZNFO and its DNA binding element was confirmed to be essential for its repressive function through a luciferase-based reporter assay.
Article
Clinical Neurology
Charles A. Galea, Aamira Huq, Paul J. Lockhart, Geneieve Tai, Louise A. Corben, Eppie M. Yiu, Lyle C. Gurrin, David R. Lynch, Sarah Gelbard, Alexandra Durr, Francoise Pousset, Michael Parkinson, Robyn Labrum, Paola Giunti, Susan L. Perlman, Martin B. Delatycki, Marguerite V. Evans-Galea
ANNALS OF NEUROLOGY
(2016)
Meeting Abstract
Biotechnology & Applied Microbiology
Sze Hwee Ong, Dong C. Zhang, Chou H. Sim, Matthew Burton, Dean Phelan, Sarah E. M. Stephenson, Gabrielle R. Wilson, Donald F. Newgreen, Anthony J. Hannan, Paul J. Lockhart, Martin B. Delatycki, Marguerite V. Evans-Galea
Article
Biochemistry & Molecular Biology
Mark E. Corkins, Stevin Wilson, Jean-Christophe Cocuron, Ana P. Alonso, Amanda J. Bird
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Editorial Material
Biochemistry & Molecular Biology
Amanda J. Bird, Simon Labbe
MOLECULAR MICROBIOLOGY
(2017)
Meeting Abstract
Biophysics
Vibhuti Wadhwa, Amanda J. Bird, Mark P. Foster
BIOPHYSICAL JOURNAL
(2018)
Article
Genetics & Heredity
Sangyong Choi, Ya-Mei Hu, Mark E. Corkins, Amy E. Palmer, Amanda J. Bird
Article
Biochemistry & Molecular Biology
Ya-Mei Hu, Derek M. Boehm, Hak Chung, Stevin Wilson, Amanda J. Bird
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Stevin Wilson, Yi-Hsuan Liu, Carlos Cardona-Soto, Vibhuti Wadhwa, Mark P. Foster, Amanda J. Bird
MOLECULAR MICROBIOLOGY
(2019)
Review
Biochemistry & Molecular Biology
Amanda J. Bird, Stevin Wilson
CURRENT OPINION IN CHEMICAL BIOLOGY
(2020)
Review
Food Science & Technology
Siqiong Zhong, Amanda Bird, Rachel E. Kopec
Summary: Chlorophyll is responsible for the green color of plant leaves and can be consumed through green vegetables. Metallo-chlorophyll derivatives have the ability to deliver essential metals and bind toxins, primarily during digestion. There are still gaps in understanding the metabolism and bioactivity of metal and toxin-binding properties of this family of molecules.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Meeting Abstract
Biotechnology & Applied Microbiology
Sze Hwee Ong, Dong C. Zhang, Chou H. Sim, M. Burton, Dean Phelan, Sarah E. M. Stephenson, Gabrielle R. Wilson, Donald F. Newgreen, Anthony J. Hannan, Paul J. Lockhart, Martin B. Delatycki, Marguerite V. Evans-Galea
JOURNAL OF GENE MEDICINE
(2018)
Meeting Abstract
Biotechnology & Applied Microbiology
Sze Hwee Ong, Dong C. Zhang, Chou H. Sim, Matthew Burton, Dean Phelan, Sarah E. M. Stephenson, Gabrielle R. Wilson, Donald F. Newgreen, Anthony J. Hannan, Paul J. Lockhart, Martin B. Delatycki, Marguerite V. Evans-Galea
Article
Cell Biology
Duncan E. Crombie, Claire L. Curl, Antonia J. A. Raaijmakers, Priyadharshini Sivakumaran, Tejal Kulkarni, Raymond C. B. Wong, Itsunari Minami, Marguerite V. Evans-Galea, Shiang Y. Lim, Lea Delbridge, Louise A. Corben, Mirella Dottori, Norio Nakatsuji, Ian A. Trounce, Alex W. Hewitt, Martin B. Delatycki, Martin F. Pera, Alice Pebay
Meeting Abstract
Biotechnology & Applied Microbiology
S. H. Ong, D. C. Zhang, C. H. Sim, M. Burton, D. Phelan, S. E. M. Stephenson, G. R. Wilson, D. F. Newgreen, A. J. Hannan, P. J. Lockhart, M. B. Delatycki, M. V. Evans-Galea
HUMAN GENE THERAPY
(2016)
Meeting Abstract
Chemistry, Multidisciplinary
Amanda Bird
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2016)