Article
Food Science & Technology
Liping Zheng, Changzheng Shi, Wenjie Ma, Zhaoxin Lu, Libang Zhou, Ping Zhang, Xiaomei Bie
Summary: This study evaluated the effect of glucose concentration on the biofilm formation of Salmonella typhimurium. The results showed that low-sugar conditions were more conducive to biofilm formation, except for strain ST19. As glucose concentration increased, biofilm formation increased. Other strains stopped forming biofilms at a glucose concentration of 1.5%, but ST19 still had strong biofilm formation ability. ST19 adapted to a high-glucose environment by regulating glucose transport and metabolism, and regulated biofilm formation through upregulation of genes in the two-component system.
Article
Biotechnology & Applied Microbiology
Yan Shi, Hao Peng, Yuying Liao, Jun Li, Yangyan Yin, Hongyan Peng, Leping Wang, Yizhou Tan, Changting Li, Huili Bai, Chunxia Ma, Wenbao Tan, Xun Li
Summary: The preventive mechanism of Lentilactobacillus buchneri (L. buchneri) against Salmonella infection in vivo was studied. L. buchneri alleviated symptoms caused by Salmonella infection, enhanced mucosal barrier, improved absorptive capacity, reduced intestinal inflammation, and modulated gut microbiota.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Biology
Gita Naseri, Hannah Raasch, Emmanuelle Charpentier, Marc Erhardt
Summary: In this study, a set of arabinose-inducible artificial transcription factors (ATFs) were developed using CRISPR/dCas9 and Arabidopsis-derived DNA-binding proteins to control gene expression in E. coli and Salmonella over a wide range of inducer concentrations. The transcriptional output of the ATFs varied greatly, particularly in Salmonella strains rewired for arabinose catabolism. The developed ATFs were used to engineer a Salmonella biosensor strain and regulate beta-carotene biosynthesis in E. coli, resulting in significantly higher production compared to traditional methods.
COMMUNICATIONS BIOLOGY
(2023)
Article
Immunology
Anne-Marie Chausse, Sylvie M. Roche, Marco Moroldo, Christelle Hennequet-Antier, Sebastien Holbert, Florent Kempf, Emilie Barilleau, Jerome Trotereau, Philippe Velge
Summary: This study describes the transcriptomic reprogramming of IEC-6 intestinal epithelial cell line to Salmonella Typhimurium strains that invaded cells by trigger or zipper mechanisms. Regardless of the entry mechanism, the transcriptomic reprogramming of the cell was similar. However, when comparing gene expression between exposed and unexposed cells, the transcriptomic reprogramming of exposed cells was significantly different, indicating the impact of Salmonella infection. The activation of target genes of the aryl hydrocarbon receptor and modulation of extracellular matrix genes were observed during Salmonella infection.
Article
Agriculture, Dairy & Animal Science
H. Xue, D. Wang, B. M. Hargis, G. Tellez-Isaias
Summary: The reduction in antibiotic use in poultry has created a need for natural solutions to control pathogens like Salmonella. NeutraPath, a natural feed additive, was found to inhibit the growth of Salmonella in vitro and reduce its colonization in broilers in vivo. It also downregulated the expression of virulence genes and preserved intestinal barrier integrity.
Article
Microbiology
Jirachaya Toyting, Nami Miura, Fuangfa Utrarachkij, Wimonrat Tanomsridachchai, Lawrence P. Belotindos, Pondpan Suwanthada, Thoko Flav Kapalamula, Siriporn Kongsoi, Kentaro Koide, Hyun Kim, Jeewan Thapa, Chie Nakajima, Yasuhiko Suzuki
Summary: Quinolone-resistant nontyphoidal Salmonella is a global public health concern, and WQ-3034 and WQ-3154 show potential as effective therapeutic agents against it.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Pantu Kumar Roy, Min Gyu Song, Shin Young Park
Summary: Quercetin, an active nutraceutical element, exhibits inhibitory effects against Salmonella Typhimurium on surfaces by disrupting biofilms and suppressing the expression of virulence and stress response genes, making it a potential antibiofilm agent in the food industry.
Article
Chemistry, Medicinal
Maher N. Alandiyjany, Ahmed S. Abdelaziz, Ahmed Abdelfattah-Hassan, Wael A. H. Hegazy, Arwa A. Hassan, Sara T. Elazab, Eman A. A. Mohamed, Eman S. El-Shetry, Ayman A. Saleh, Naser A. ElSawy, Doaa Ibrahim
Summary: This study demonstrates the efficacy of ciprofloxacin-loaded mesoporous silica nanoparticles in treating Salmonella Typhimurium infection. The nanoparticles are able to control the release of ciprofloxacin, leading to prolonged antibacterial effects and reduced biofilm formation. In addition, oral supplementation of the nanoparticles has no significant adverse effects and can attenuate inflammation and cell apoptosis caused by S. typhimurium.
Article
Veterinary Sciences
Elisabetta Razzuoli, Chiara Grazia De Ciucis, Barbara Chirullo, Katia Varello, Roberto Zoccola, Lisa Guardone, Paola Petrucci, Danja Rubini, Elena Bozzetta, Maria Goria, Floriana Fruscione, Paola Modesto
Summary: Canine mammary cancer shares many similarities with human breast cancer in terms of risk factors, behavior, and clinical course. These breast tumors are resistant to conventional therapies, making the study of alternative treatments necessary. The CF33 cell line can serve as an in vitro model for evaluating innovative therapeutic approaches involving bacteria.
VETERINARY SCIENCES
(2022)
Article
Multidisciplinary Sciences
Chanmi Kim, Iqra Latif, Durga P. Neupane, Gi Young Lee, Ryan S. Kwon, Alia Batool, Qasim Ahmed, Muhammad Usman Qamar, Jeongmin Song
Summary: This study conducted antibiotic-resistance profiling of XDR S. Typhi isolates from pediatric patients with septicemia, revealing a match between the phenotype and genotype of the isolates. The findings provide insights into the development of molecular detection methods and treatment strategies for XDR S. Typhi.
Article
Microbiology
Zengfeng Zhang, Jiang Chang, Xuebin Xu, Mengjun Hu, Shoukui He, Xiaojie Qin, Min Zhou, Chunlei Shi, Xianming Shi
Summary: Salmonella enterica serovar Indiana has become increasingly prevalent in China as a foodborne pathogen. This study investigated the national prevalence and genetic characterization of this pathogen. The study found that the ST17 clone of S. Indiana is widely distributed in China, and is highly resistant to multiple antibiotics. The genetic analysis revealed the close relationship between ST17 isolates in China and those from other countries. These findings highlight the importance of surveillance and control measures against the high-resistant ST17 clone.
MICROBIOLOGY SPECTRUM
(2022)
Review
Multidisciplinary Sciences
Candice R. Gurbatri, Nicholas Arpaia, Tal Danino
Summary: With the use of synthetic biology tools, researchers are repurposing bacteria as tumor-specific delivery systems, which can modulate the tumor microenvironment and enhance therapeutic efficacy and safety through their immunogenicity and local payload production.
Article
Microbiology
Muhammad Zakir, Maryam Khan, Muhammad Ihtisham Umar, Ghulam Murtaza, Muhammad Ashraf, Saba Shamim
Summary: This study in a local hospital in Lahore found a high prevalence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Salmonella Typhi, with males and children aged 0-10 being more affected. The incidence of XDR S. Typhi was alarmingly high in both the outpatient department and general ward. The resistant strains showed similar resistance patterns across different wards and patient types, with piperacillin/tazobactam being the most effective antibiotic observed.
Article
Microbiology
Ahmed Esmael, Ehab Azab, Adil A. Gobouri, Mohamed A. Nasr-Eldin, Mahmoud M. A. Moustafa, Shereen A. Mohamed, Omnia A. M. Badr, Alzahraa M. Abdelatty
Summary: Foodborne salmonellosis is a global threat, and in this study, two broad-spectrum lytic Salmonella phages, SPHG1 and SPHG3, were isolated and characterized for their ability to infect multidrug-resistant Salmonella Typhimurium. These phages showed strong lytic activity with a short lysis time and high burst sizes, and were stable under various environmental conditions. A phage cocktail containing SPHG1 and SPHG3 significantly reduced the viable count of S. Typhimurium in milk, water, and chicken breast, demonstrating their potential as biocontrol agents against foodborne salmonellosis.
Article
Food Science & Technology
Pratheep Sandrasaigaran, Shuvarnah Mohan, Nithiyha Sandara Segaran, Tze Yan Lee, Son Radu, Hanan Hasan
Summary: This study investigated the prevalence of antimicrobial-resistant Salmonella carried by filth flies at wet markets, highlighting the potential threat to public health. It found that a significant percentage of flies harbored antimicrobial-resistant Salmonella, with some strains displaying high resistance and virulence. These findings provide valuable insights into controlling the spread and outbreak of foodborne diseases.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)