Article
Immunology
Ching-Lien Wu, Julien Caumartin, Giada Amodio, Francois Anna, Maria Loustau, Silvia Gregori, Pierre Langlade-Demoyen, Joel LeMaoult
Summary: Invariant Natural Killer T (iNKT) cells, when activated by exogenic glycolipid antigen, can release cytokines rapidly to enhance anti-tumor activity. The presence of the tolerogenic molecule HLA-G in humans may impact the function of iNKT cells, potentially affecting the outcome of clinical trials.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Abdur Rashid, Li Kang, Feng Yi, Fatima Mir, Yimam Getaneh, Yiming Shao, Syed Hani Abidi
Summary: This study identified a unique recombinant cluster composed of CRF02_AG, sub-subtype A3, and subtype G among HIV-infected children in Larkana. The presence of multiple co-circulating lineages in Larkana suggests the generation of new circulating recombinant forms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Plant Sciences
Shi-Jian Song, Hai-Ping Diao, Byeongho Moon, Areum Yun, Inhwan Hwang
Summary: Plants are a cost-effective platform for recombinant production and have the advantage of low risk of contamination of human pathogens. This study demonstrates the versatility of the B1 domain of Streptococcal protein G (GB1) as a fusion tag for recombinant protein production in plants. Fusion with the GB1 domain increases expression levels and stability of fusion proteins, while direct detection using secondary anti-IgG antibody simplifies analysis. Therefore, the small GB1 domain can be used as a versatile tag for recombinant protein production in plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Jose Luis Corchero, Marianna T. P. Favaro, Merce Marquez-Martinez, Jara Lascorz, Carlos Martinez-Torro, Julieta M. Sanchez, Hector Lopez-Laguna, Luis Carlos de Souza Ferreira, Esther Vazquez, Neus Ferrer-Miralles, Antonio Villaverde, Eloi Parlade
Summary: By using protein engineering techniques, recombinant proteins can be assembled into functional materials such as nanoparticles or secretory microparticles for drug delivery purposes. The use of histidine-rich tags and divalent cations allows the construction of these materials from pure polypeptide samples. The selection of the protein biofabrication platform is crucial in determining the biophysical properties of the resulting products.
Article
Parasitology
Nengxing Shen, Yuhang Chen, Wenrui Wei, Lang Xiong, Yuanyuan Tao, Jie Xiao, Song Liu, Xue He, Xiaodi Du, Xiaobin Gu, Yue Xie, Jing Xu, Xuerong Peng, Guangyou Yang
Summary: The study found that IgE antibodies to rSsCLP5 and rSsCLP12 have high sensitivity and specificity for diagnosing scabies. Additionally, both rSsCLP5 and rSsCLP12 exhibit allergenic properties, with rSsCLP12 being a stronger allergen than rSsCLP5.
PARASITES & VECTORS
(2021)
Article
Astronomy & Astrophysics
Tayyaba Naz, Adnan Malik, M. Kamran Asif, Iffat Fayyaz
Summary: The objective of this paper is to explore evolving traversable wormholes using modified gravity theory. The authors develop a wormhole shape function and analyze the energy conditions and stability of the wormhole configurations. The findings suggest that the chosen gravity models yield validated and stable wormhole configurations.
PHYSICS OF THE DARK UNIVERSE
(2023)
Review
Endocrinology & Metabolism
Jurgen Wess
Summary: GPCRs are significant targets for drugs approved by the US FDA, where activation of different GPCRs can impact physiological outcomes differently, reflecting the effects of G protein activation from different perspectives.
Review
Biotechnology & Applied Microbiology
Filipe Silva, Sara Santos, Roberto Meyer, Eduardo Silva, Carina Pinheiro, Neuza Alcantara-Neves, Luis Pacheco
Summary: The article discusses methods for producing soluble untagged proteins in Escherichia coli using genetic engineering techniques, which involve intracellular processing through co-expression of a specific protease. This approach simplifies the purification of recombinant proteins, improves the solubility of target proteins, and facilitates screening for proteins that remain soluble after tag removal.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Multidisciplinary Sciences
Irene Vorontsova, Alexander Vallmitjana, Belen Torrado, Thomas F. Schilling, James E. Hall, Enrico Gratton, Leonel Malacrida
Summary: Macromolecular crowding and water dynamics play important roles in cellular homeostasis and fate determination. Understanding macromolecular crowding in the lens is crucial for preventing eye diseases such as cataract and presbyopia. This study combines the use of a nanoenvironmental sensor and zebrafish mutants to investigate the spatial regulation of macromolecular crowding and the roles of specific aquaporin proteins in the lens.
Article
Medicine, Research & Experimental
Sadiya Bi Shaikh, Mohd Altaf Najar, Thottethodi Subrahmanya Keshava Prasad, Yashodhar P. Bhandary
Summary: The study assessed the involvement of IL-17A during replication stress and found that curcumin can suppress the expression of MCM proteins. This was confirmed using mass spectrometry and molecular techniques, with curcumin also shown to inhibit mRNA levels of other MCM proteins. This study enhances our understanding of the link between replication stress and pro-inflammatory effects, providing insights for potential ALI therapies.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Hannah Schaetzle, Eva-Maria Brouwer, Elisa Liebhart, Mara Stevanovic, Enrico Schleiff
Summary: Porins play essential roles in the viability of Gram-negative bacteria by facilitating nutrient uptake and maintaining outer membrane integrity. In this study, the function of porins in the cyanobacterium Anabaena sp. PCC 7120 was explored, revealing their involvement in salt, copper, zinc, and manganese transport. Notably, porins also appear to be associated with envelope integrity and solute uptake in this organism.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Omarine N. Nlinwe, Ebenezer A. Ofori, Kwadwo Akyea-Mensah, Eric Kyei-Baafour, Harini Ganeshan, Maria Belmonte, Bjoern Peters, Eileen Villasante, Martha Sedegah, Kwadwo Asamoah Kusi
Summary: Antigen polymorphisms in essential malarial antigens pose challenges to vaccine design, with antibody responses well studied but fewer studies on T cell responses. This study found evidence of polymorphisms affecting T cell response induction in AMA1 gene, but other factors like TCR recognition of HLA-peptide complexes may also play a role.
Article
Biochemistry & Molecular Biology
Fiona J. Houghton, Christian Makhoul, Ellie Hyun-Jung Cho, Nicholas A. Williamson, Paul A. Gleeson
Summary: There is a highly organized complex of small G proteins on TGN membranes, with Arl5b being one of its members, and Arl5b is required for TGN recruitment of ACBD3.
Article
Pathology
Thao Le Masters, Stephen Johnson, Patricio R. Jeraldo, Kerryl E. Greenwood-Quaintance, Scott A. Cunningham, Matthew P. Abdel, Nicholas Chia, Robin Patel
Summary: Transcriptomic analysis of Staphylococcus aureus in periprosthetic joint infection (PJI) revealed significant changes in gene expression related to biofilm formation, iron acquisition, and antimicrobial resistance, with no significant differences in expression between in vivo and in vitro conditions.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2021)
Article
Urology & Nephrology
Yu Xi, Elena Colonnello, Gongchao Ma, Erika Limoncin, Giacomo Ciocca, Hui Zhang, Emmanuele A. Jannini, Yan Zhang
Summary: Our study suggests that the cutoff values of both SHIM and IIEF-EF should be amended (17.5 for SHIM and 24.5 for IIEF-EF) when assessing erectile function among PE population. These adjustments aim to improve the diagnostic accuracy for erectile dysfunction in premature ejaculation patients. Further validation in larger cohorts of PE patients is needed to confirm the findings.
JOURNAL OF SEXUAL MEDICINE
(2021)
Article
Immunology
Ching-Lien Wu, Signe Goul Svendsen, Adrien Riviere, Francois Desgrandchamps, Edgardo D. Carosella, Joel LeMaoult
Article
Immunology
Signe Goul Svendsen, Maja Soberg Udsen, Marina Daouya, Tina Funck, Ching-Lien Wu, Edgardo D. Carosella, Joel LeMaoult, Thomas Vauvert F. Hviid, Carsten Faber, Mogens Holst Nissen
Article
Oncology
Diana Tronik-Le Roux, Julie Renard, Jerome Verine, Victor Renault, Emmanuel Tubacher, Joel LeMaoult, Nathalie Rouas-Freiss, Jean-Francois Deleuze, Francois Desgrandschamps, Edgardo D. Carosella
MOLECULAR ONCOLOGY
(2017)
Meeting Abstract
Oncology
C. Hennequin, M. Daouya, D. Tronik-Le Roux, J. LeMaoult, N. Rouas-Freiss, F. Desgrandchamps, E. Carosella
RADIOTHERAPY AND ONCOLOGY
(2017)
Article
Cell & Tissue Engineering
A. Verloes, C. Spits, M. Vercammen, M. Geens, J. LeMaoult, K. Sermon, W. Coucke, H. Van de Velde
STEM CELL RESEARCH
(2017)
Article
Oncology
Nathalie Rouas-Freiss, Joel LeMaoult, Jerome Verine, Diana Tronik-Le Roux, Stephane Culine, Christophe Hennequin, Francois Desgrandchamps, Edgardo D. Carosella
Article
Immunology
Aparna Sundaresh, Ching-Lien Wu, Raj Kumar Chinnadurai, Ravi Philip Rajkumar, Christina Mary Mariaselvam, Joel LeMaoult, Rajagopal Krishnamoorthy, Marion Leboyer, Vir Singh Negi, Ryad Tamouza
IMMUNOLOGICAL INVESTIGATIONS
(2018)
Article
Cell Biology
S. G. Svendsen, L. L. Nilsson, S. Djurisic, T. Funck, C. -L. Wu, C. Faber, M. K. Falk, A. Singh, T. L. Sorensen, E. D. Carosella, J. LeMaoult, T. V. F. Hviid, M. H. Nissen
Article
Immunology
Catherine A. Lombard, Gwenaelle Sana, Joel LeMaoult, Mehdi Najar, Joachim Ravau, Floriane Andre, Fatima Bouhtit, Marina Daouya, Maria Loustau, Mustapha Najimi, Laurence Lagneaux, Edgardo D. Carosella, Etienne M. Sokal
JOURNAL OF IMMUNOLOGY RESEARCH
(2019)
Article
Oncology
Diana Tronik-Le Roux, Mathilde Sautreuil, Mahmoud Bentriou, Jerome Verine, Maria Belen Palma, Marina Daouya, Fatiha Bouhidel, Sarah Lemler, Joel LeMaoult, Francois Desgrandchamps, Paul-Henry Cournede, Edgardo D. Carosella
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2020)
Article
Cell Biology
Guillaume Mestrallet, Frederic Auvre, Chantal Schenowitz, Edgardo D. Carosella, Joel LeMaoult, Michele T. Martin, Nathalie Rouas-Freiss, Nicolas O. Fortunel
Summary: Research has shown that human epidermal keratinocytes can protect the body against infection and injury by inhibiting the proliferation of immune cells under normal and inflammatory conditions. This inhibition is achieved through the secretion of soluble factors and the cell-surface expression of HLA-G1 and PD-L1 immune checkpoints.
Article
Multidisciplinary Sciences
Maria Belen Palma, Diana Tronik-Le Roux, Guadalupe Amin, Sheila Castaneda, Alan M. Mobbs, Maria Agustina Scarafia, Alejandro La Greca, Marina Daouya, Isabelle Poras, Ana Maria Inda, Lucia N. Moro, Edgardo D. Carosella, Marcela N. Garcia, Santiago G. Miriuka
Summary: The study successfully achieved downregulation of HLA-G expression in tumor cells using CRISPR/Cas9 gene editing, leading to enhanced in vitro immune cell response. This finding provides an important foundation for the development of novel clinical immunotherapeutic approaches in cancer.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Diana Tronik-Le Roux, Marina Daouya, Alix Jacquier, Chantal Schenowitz, Francois Desgrandchamps, Nathalie Rouas-Freiss, Edgardo D. Carosella
Summary: The heterogeneity of cancer cells poses challenges in therapeutic approaches, and the newly discovered isoform HLA-G increment alpha 1 has immune-stimulatory activity in ccRCC and trophoblasts. Unlike other HLA-G isoforms, HLA-G increment alpha 1 does not associate with beta 2-microglobulin and may not present antigens to T cells. It exerts individual dependent immune-stimulatory effects on peripheral blood NK cells and might have potential antitumor effects.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Victoria Michaels, Smahane Chalabi, Agnes Legrand, Julie Renard, Emmanuel Tejerina, Marina Daouya, Sylvie Fabrega, Jerome Megret, Robert Olaso, Anne Boland, Jean-Francois Deleuze, Christophe Battail, Diana Tronik-Le Roux, Sophie Ezine
Summary: T cells have the potential to maintain immunological memory and self-tolerance. Delayed T cell reconstitution is a common issue in hematopoietic stem cell transplantation. To overcome this difficulty, a new approach using DNA barcoding strategy was developed to identify populations with efficient lymphoid reconstitution properties. Results highlight the role of LMPP progenitors for lymphoid generation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)