Article
Chemistry, Applied
Mengmeng Wang, Qinghua Liu, Fen Li, Jiawei Tang, Xuesong Xiong, Yingying Yang, Pei Ju, Ziyi Wang, Robert G. Gilbert, Liang Wang
Summary: This study identified both fragile and stable glycogen particles in Escherichia coli, with the structure showing dynamic changes during synthesis and degradation stages. Molecular analysis suggested that the fragility of glycogen particles in bacteria may be influenced by the balance of gene expression levels in glycogen metabolism.
CARBOHYDRATE POLYMERS
(2021)
Article
Multidisciplinary Sciences
Stefan Kittler, Christoph Slouka, Andreas Pell, Roman Lamplot, Mihail Besleaga, Sarah Ablasser, Christoph Herwig, Oliver Spadiut, Julian Kopp
Summary: Continuous processing is the golden standard in many industrial sectors for maximizing productivity, but subpopulation formation when working with living cells can lead to instabilities in long-term cultivations. Cascaded continuous cultivation allows spatial separation of biomass formation and recombinant protein expression, facilitating stable protein expression using microbial hosts. This study presents a method workflow based exclusively on bioengineering methods to reduce workload and accelerate the establishment of stable continuous processes with E. coli BL21(DE3).
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Thais Barros, Larissa Brumano, Marcela Freitas, Adalberto Pessoa, Nadia Parachin, Perola O. Magalhaes
Summary: This study aimed to produce L-asparaginase from Escherichia coli for the treatment of acute lymphocytic leukemia. The defined medium showed the highest enzyme activity. Batch and fed-batch cultures were conducted to achieve high cell density and increase enzyme productivity through lactose induction.
Article
Electrochemistry
Jiyun Baek, Changman Kim, Young Eun Song, Da Seul Kong, Sakuntala Mutyala, Eun-Hee Seol, Jung Rae Kim
Summary: This paper reports an electrode-assisted non-native pathway regulation using a potentiostat to induce a change in intracellular redox state via bacteria-electrode electron transfer. By controlling the electrode potential, the production of 3-hydroxypropionic acid or 1,3-propanediol in E. coli was increased or enhanced, respectively.
ELECTROCHIMICA ACTA
(2022)
Article
Biotechnology & Applied Microbiology
Bingjieu Yu, Wenzhe Sun, Zhen Huang, Gang Sun, Le Li, Jiawei Gu, Mengying Zheng, Xiaokun Li, ChangJu Chun, Qi Hui, Xiaojie Wang
Summary: The study successfully cloned and expressed the human aFGF gene in E. coli, reaching a high production scale of 30 L with efficient purification. Results demonstrate that rhaFGF(135) retains potent mitogenic activity and holds great potential for wide-ranging applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biology
Sophia A. H. Heyde, Morten H. H. Norholm
Summary: The study uncovered the crucial role of RNAse E in the toxicity caused by overexpression of heterologous proteins in E. coli, highlighting RNA stability as a key factor in gene expression toxicity. Evolution of a mutant strain with a truncation in RNAse E suggests that targeting RNases could lead to improved protein production in cell factories, challenging the existing assumptions about certain E. coli strains.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Pengjun Han, Huahao Fan, Yigang Tong
Summary: A novel family B DNA polymerase, IME199 DNAP, was successfully overproduced in Escherichia coli. IME199 DNAP exhibited 3'-5' exonuclease activity, dNTP-dependent 5'-3' polymerase activity, strand displacement and processive synthesis capabilities. It can perform DNA amplification at room temperature with very low error rates.
MICROBIAL CELL FACTORIES
(2023)
Article
Microbiology
Yinfeng Wang, Guanhua Xuan, Houqi Ning, Jiuna Kong, Hong Lin, Jingxue Wang
Summary: A high-throughput method combining Tn5 transposon mutation and phage screening was successfully used to obtain phage-resistant strains of Escherichia coli BL21 (DE3). Mutant strains PR281-7, PR338-8, PR339-3, PR340-8, and PR347-9 were obtained, which effectively resisted phage infection and maintained the capability of producing recombinant proteins. Comparative genomics analysis revealed mutated genes in ecpE, nohD, nrdR, and livM in these phage-resistant strains.
JOURNAL OF MICROBIOLOGY
(2023)
Article
Agricultural Engineering
Shunqing Xu, Bin Zhang, Wanhe Chen, Kai Ye, Jian Shen, Pengfu Liu, Jiequn Wu, Hong Wang, Xiaohe Chu
Summary: This article describes the development of a microbial cell factory in Escherichia coli that produces ectoine at high titers, aiming to develop efficient and environmentally friendly bio-based ectoine production technology. The research provides important achievements and potential for industrial applications.
BIORESOURCE TECHNOLOGY
(2023)
Article
Microbiology
Yanfei Xia, Shen Li, Guohui Xu, Shanshan Xie, Xueting Liu, Xiaomin Lin, Huijun Wu, Xuewen Gao
Summary: The study evaluated the nematicidal effects of Escherichia coli BL21(DE3) against Meloidogyne javanica and identified the genes associated with nematicidal activity. The results suggest that BL21(DE3) is a potential microorganism for the continuable biological control of root-knot nematode in tomato and that the carB gene modulates the pH environment to affect the nematicidal activity of BL21(DE3).
Article
Biochemical Research Methods
Maxwell Neal, Deepan Thiruppathy, Karsten Zengler
Summary: This study developed a genome-scale metabolic model of B. fragilis strain 638R, providing a basis for understanding its relationship with human diet and metabolic products.
PLOS COMPUTATIONAL BIOLOGY
(2023)
Article
Microbiology
Bing Fu, Junhui Ying, Qingwei Chen, Qili Zhang, Jiajie Lu, Zhiwen Zhu, Ping Yu
Summary: In this study, the riboflavin-producing strain R1 was constructed by overexpressing five key genes in Escherichia coli BL21. The R2 strain was then constructed by overexpressing another gene in the R1 strain, resulting in an increase of riboflavin production. The deletion of a riboswitch gene in E. coli BL21 further enhanced riboflavin production, leading to the generation of the R4 strain. The results suggest that these genetic modifications can greatly improve riboflavin production.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yue Wang, Disheng Zhou, Hongshun Yang
Summary: In recent years, there have been increasing outbreaks of six major Escherichia coli strains linked to wheat flour consumption, highlighting the importance of adopting antimicrobial treatment during flour production. This study evaluated the effectiveness of thermal treatment, a typical sanitizing approach, against these six strains in wheat flour and explored the underlying antimicrobial mechanisms. The findings showed that thermal treatment at 60 and 70 degrees C significantly reduced the loads of all strains in wheat flour. Additionally, activated heat shock protein synthesis, expedited glycolysis, and enhanced osmotic protection were identified as major metabolic alteration patterns in the E. coli strains to cope with heat stress. These findings provide a scientific basis for incorporating a thermal inactivation step in wheat flour production.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Stefano Camborda, Jan-Niklas Weder, Nadine Toepfer
Summary: CobraMod is a Python package for pathway-centric modification and extension of genome-scale metabolic networks. It integrates data from different metabolic pathway databases and user-curated information, and provides high quality metabolic reconstructions.
Article
Biochemical Research Methods
Akos Vegvari, Xuepei Zhang, Roman A. Zubarev
Summary: This study successfully detected proteins from individual Escherichia coli bacteria for the first time, with validation of the findings through comparison with other samples and bulk proteomics data.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Biochemistry & Molecular Biology
Constanze Pinske, Frank Sargent, R. Gary Sawers
Article
Microbiology
Constanze Pinske, Frank Sargent
Article
Microbiology
Monique Jaroschinsky, Constanze Pinske, R. Gary Sawers
Article
Microbiology
Ciaran M. Lamont, Ciaran L. Kelly, Constanze Pinske, Grant Buchanan, Tracy Palmer, Frank Sargent
Article
Biotechnology & Applied Microbiology
Claudia Thomas, Mandy Waclawek, Kerstin Nutschan, Constanze Pinske, R. Gary Sawers
JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY
(2018)
Article
Microbiology
Constanze Pinske
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Microbiology
Nadya Dragomirova, Patricia Rothe, Stefan Schwoch, Stefanie Hartwig, Constanze Pinske, R. Gary Sawers
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Microbiology
Ute Lindenstrauss, Constanze Pinske
JOURNAL OF BACTERIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Dorothea Lubek, Andreas H. Simon, Constanze Pinske
Article
Microbiology
Constanze Pinske, Claudia Thomas, Kerstin Nutschan, R. Gary Sawers
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Philipp Skorupa, Ute Lindenstrauss, Sabrina Burschel, Christian Blumenscheit, Thorsten Friedrich, Constanze Pinske
Article
Biochemistry & Molecular Biology
Lusine Karapetyan, Constanze Pinske, Gary Sawers, Armen Trchounian, Karen Trchounian
Article
Chemistry, Inorganic & Nuclear
Janik Telleria Marloth, Constanze Pinske
Article
Microbiology
B. Hakobyan, C. Pinske, G. Sawers, A. Trchounian, K. Trchounian
FEMS MICROBIOLOGY LETTERS
(2018)
Article
Biochemistry & Molecular Biology
Ute Lindenstrauss, Philipp Skorupa, Jennifer S. McDowall, Frank Sargent, Constanze Pinske
BIOCHEMICAL JOURNAL
(2017)