Article
Biotechnology & Applied Microbiology
Jinpeng Yao, Yuanhua Cai, Zhixiang Chen, Xuechun Wang, Xiaolin Lai, Lili Pan, Yang Li, Shaoyuan Wang
Summary: This study revealed a new role for DHX15 in zebrafish intestinal development. We found intestinal defects, decreased proliferation, and increased apoptosis in dhx15 knockout zebrafish. RNA genome wide analysis and qRT-PCR analysis showed down-regulation of the Wnt signaling pathway in dhx15 knockout zebrafish. Therefore, we concluded that dhx15 regulates zebrafish intestinal development through the Wnt signaling pathway. This study provides new insights into the role of dhx15 in intestinal development beyond its well-characterized role in intestinal immunity.
Article
Developmental Biology
Eva Janeckova, Jifan Feng, Tingwei Guo, Xia Han, Aileen Ghobadi, Angelita Araujo-Villalba, Md Shaifur Rahman, Heliya Ziaei, Thach-Vu Ho, Siddhika Pareek, Jasmine Alvarez, Yang Chai
Summary: This study reveals the crucial role of Wnt signaling in soft palate development and muscle differentiation through the regulation of ciliary homeostasis. Wnt signaling activates ciliary gene Ttll3 expression, which is necessary for mesenchymal cell proliferation and subsequent myogenesis.
Review
Cell Biology
Ian James Bell, Matthew Sheldon Horn, Terence John Van Raay
Summary: The non-canonical Wnt signaling pathways have different roles in cancer and development, involving calcium and Vangl2. Cells tend to choose non-canonical Wnt signaling over canonical Wnt signaling when presented with a choice, but this occurs rarely in vivo.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Jessica Schaefer, Nicole Wenck, Katharina Janik, Joshua Linnert, Katarina Stingl, Susanne Kohl, Kerstin Nagel-Wolfrum, Uwe Wolfrum
Summary: Human Usher syndrome (USH) is a complex genetic disorder that causes combined deafness and blindness. The underlying mechanisms of the disease, particularly in the eye and retina, are not well understood. This study identifies a role for the scaffold protein harmonin, encoded by the USH1C gene, in regulating the canonical Wnt/beta-catenin signaling pathway. The findings suggest that USH1C/harmonin acts as a suppressor of the pathway, and dysregulation of this pathway may contribute to the development of USH.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Developmental Biology
Denver D. Britto, Jia He, June P. Misa, Wenxuan Chen, Purvi M. Kakadia, Lin Grimm, Caitlin D. Herbert, Kathryn E. Crosier, Philip S. Crosier, Stefan K. Bohlander, Benjamin M. Hogan, Christopher J. Hall, Jesus Torres-Vazquez, Jonathan W. Astin
Summary: This study identifies Plexin D1 as a negative regulator of lymphatic vessel guidance and lymphangiogenesis in zebrafish. It is shown that Plxnd1 antagonises Vegfr/Erk signalling to ensure the correct number of facial LECs and prevent facial lymphatic hyperplasia. These findings provide insights into the molecular mechanisms underlying lymphatic vessel patterning and offer potential therapeutic targets for diseases associated with dysregulated lymphatic growth.
Article
Biology
Andrew M. Hamilton, Olga A. Balashova, Laura N. Borodinsky
Summary: Research indicates that Hedgehog (Hh) signaling may be a useful target for driving regeneration, and non-canonical mechanisms of Hh signaling are necessary for spinal cord and muscle regeneration.
Article
Engineering, Biomedical
Derek Avery, Lais Morandini, Luke S. Sheakley, Arth H. Shah, Loc Bui, Jefferson O. Abaricia, Rene Olivares-Navarrete
Summary: This study investigates the role of canonical Wnt signaling in the response of macrophages to titanium surface properties. The results show that activating canonical Wnt signaling promotes a pro-inflammatory phenotype, while inhibiting it enhances an anti-inflammatory response.
Article
Genetics & Heredity
Jinzhi He, Junjun Jing, Jifan Feng, Xia Han, Yuan Yuan, Tingwei Guo, Fei Pei, Yuanyuan Ma, Courtney Cho, Thach-Vu Ho, Yang Chai
Summary: The number of tooth roots is finely regulated in mammalian dentition, with Lhx6 playing a key role in determining molar root number by controlling the region-specific fate determination of progenitor cells. Loss of Lhx6 leads to root patterning defects, indicating its importance in organ morphogenesis and future tooth root regeneration strategies.
Article
Immunology
Ziyuan Lin, Min Luo, Bin Zhou, Yanyan Liu, Huaqin Sun
Summary: Studies have found that genetic ablation of CFTR in zebrafish results in inhibited embryonic T lymphocyte development, attributed to impaired thymic homing and hematopoietic stem cell expansion. Transcriptome analysis of isolated HSCs in zebrafish embryos at 48 hpf revealed significant alterations in key factors essential for T cell development and Wnt signaling.
IMMUNOLOGY LETTERS
(2021)
Article
Cell Biology
Andre Landin Malt, Shaylyn Clancy, Diane Hwang, Alice Liu, Connor Smith, Margaret Smith, Maya Hatley, Christopher Clemens, Xiaowei Lu
Summary: A non-canonical Wnt signaling pathway has been found to promote cochlear outgrowth and coordinate planar polarization of hair cells. Gsk3 beta and Rac1 play crucial roles in this pathway, as shown in genetic rescue experiments.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Tarannum Taznin, Kaushalya Perera, Yann Gibert, Alister C. Ward, Clifford Liongue
Summary: This study investigated the expression and function of CRLF3, an evolutionarily significant protein, in early development, particularly in blood and immune cells, using zebrafish as a model. The study found that CRLF3 was expressed in multiple embryonic tissues and played a crucial role in primitive hematopoiesis and early definitive hematopoiesis.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Silvia Muccioli, Valentina Brillo, Leonardo Chieregato, Luigi Leanza, Vanessa Checchetto, Roberto Costa
Summary: The Wnt signaling pathway is crucial in embryonic development for controlling cell proliferation, but aberrant activation in human diseases has been observed. Ion channels, known as modulators of important cellular functions, have been widely researched for their influence on Wnt signaling regulation. This review focuses on the links between ion channels and canonical Wnt signaling, discussing their potential roles in human metabolic diseases, neurological disorders, and cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Koki Kimura, Shiori Yamamori, Masaharu Hazawa, Jingjing Kobayashi-Sun, Mao Kondo, Richard W. Wong, Isao Kobayashi
Summary: Using a stromal cell line from the zebrafish kidney, researchers found that an inhibitor of canonical Wnt signaling, IWR-1-endo, is a potent regulator of hematopoietic stem cells (HSCs). The stromal cells weakly expressed HSC maintenance factors but highly expressed canonical Wnt ligand genes. Adding IWR-1-endo or inhibiting canonical Wnt signaling increased the number of HSCs.
Article
Biochemistry & Molecular Biology
Bindu Chandrasekharan, Claudia Montllor-Albalate, Alyson E. Colin, Joshua L. Andersen, Young C. Jang, Amit R. Reddi
Summary: Cu/Zn superoxide dismutase (Sod1) catalyzes the breakdown of superoxide radicals into oxygen and hydrogen peroxide, which acts as a signaling molecule. In yeast, Sod1 is involved in a redox signaling pathway that connects nutrient availability to energy metabolism control. This pathway stabilizes plasma membrane kinases Yck1/2, promoting glycolytic growth and repressing respiration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Gang-Ho Yoon, Kyuhee Kim, Dong-Seok Park, Sun-Cheol Choi
Summary: The negative regulation of RNF152 on the Wnt/beta-catenin pathway during Xenopus early embryogenesis has been discovered. RNF152 can inhibit Wnt signaling by suppressing the polymerization of Dishevelled, which is crucial for Wnt signal transduction, to control neural crest formation in the embryo.
Article
Developmental Biology
Ding Ye, Huaping Xie, Bo Hu, Fang Lin
Article
Biochemistry & Molecular Biology
Zhichao Mei, Dawei Zhang, Bo Hu, Jing Wang, Xian Shen, Wuhan Xiao
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Marine & Freshwater Biology
Jianling Bi, Bo Hu, Jinsong Zheng, Jingzhen Wang, Wuhan Xiao, Ding Wang
Article
Multidisciplinary Sciences
Jingzhen Wang, Xueying Yu, Bo Hu, Jinsong Zheng, Wuhan Xiao, Yujiang Hao, Wenhua Liu, Ding Wang
SCIENTIFIC REPORTS
(2015)
Article
Biochemistry & Molecular Biology
Xing Liu, Xiaolian Cai, Bo Hu, Zhichao Mei, Dawei Zhang, Gang Ouyang, Jing Wang, Wei Zhang, Wuhan Xiao
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Multidisciplinary Sciences
Yu Chen, Chi Zhou, Wei Ji, Zhichao Mei, Bo Hu, Wei Zhang, Dawei Zhang, Jing Wang, Xing Liu, Gang Ouyang, Jiangang Zhou, Wuhan Xiao
NATURE COMMUNICATIONS
(2016)
Article
Oncology
Jianling Bi, Bo Hu, Jing Wang, Xing Liu, Jinsong Zheng, Ding Wang, Wuhan Xiao
Article
Developmental Biology
Bo Hu, Yuanyuan Gao, Lauren Davies, Stephanie Woo, Jacek Topczewski, Jason R. Jessen, Fang Lin
Article
Developmental Biology
Bo Hu, Wei Zhang, Xi Feng, Wei Ji, Xunwei Xie, Wuhan Xiao
DEVELOPMENTAL BIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Xiaoyang Wan, Bo Hu, Jing-xia Liu, Xi Feng, Wuhan Xiao
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Oncology
X. Sun, B. Lu, B. Hu, W. Xiao, W. Li, Z. Huang
BLOOD CANCER JOURNAL
(2014)
Article
Developmental Biology
Anurag Kakkerla Balaraju, Bo Hu, Juan J. Rodriguez, Matthew Murry, Fang Lin
Summary: The study found that endodermal cells in zebrafish exhibit mediolateral polarization during convergence and extension. Lack of glypican 4, which promotes Wnt/PCP signaling, results in loss of endodermal cell polarity, impaired intercalation, and increased expression of Cadherin 2 on the endodermal cell surface due to impaired Rab5c-mediated endocytosis. These findings demonstrate a new mechanism by which Gpc4 regulates planar cell polarity and highlight the role of Wnt/PCP signaling in endoderm morphogenesis.
Article
Developmental Biology
Benjamin C. Stark, Yuanyuan Gao, Diane S. Sepich, Lakyn Belk, Matthew A. Culver, Bo Hu, Marlene Mekel, Wyndham Ferris, Jimann Shin, Lilianna Solnica-Krezel, Fang Lin, John A. Cooper
Summary: Analysis of zebrafish carrying mutations of carmil3 revealed impaired endodermal and DFC migration during gastrulation, affecting KV morphogenesis. Mutant KVs displayed smaller size, reduced cell numbers, and decreased cilia, leading to defects in L/R patterning with varying penetrance and expressivity. These findings suggest a new role for CARMIL3 in vertebrate embryo morphogenesis, involving endodermal cell and DFC migration, as well as KV and L/R asymmetry formation.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Bo Hu, Juan J. Rodriguez, Anurag Kakkerla Balaraju, Yuanyuan Gao, Nhan T. Nguyen, Heston Steen, Saeb Suhaib, Songhai Chen, Fang Lin
Summary: The study in zebrafish reveals that Glypican 4 (Gpc4) influences signaling pathways by regulating morphogen traffic and reception, mediating Wnt5b and Wnt11f2 transport via signaling filopodia. This mechanism provides a new insight into the in vivo distribution of morphogens involving Gpc4.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Harsha Mahabaleshwar, P. Asharani, Tricia Yi Loo, Shze Yung Koh, Melissa R. Pitman, Samuel Kwok, Jiajia Ma, Bo Hu, Fang Lin, Xue Li Lok, Stuart M. Pitson, Timothy E. Saunders, Tom J. Carney
Summary: Mesenchymal cell immigration into growing fins and limb buds drives distal outgrowth, with tensile forces between these cells essential for fin and limb morphogenesis. Morphogens derived from the apical fin domain regulate cell polarity, migration, division, and adhesion of limb mesenchyme cells. The mutant stomp in zebrafish displays defects in fin morphogenesis, including blister formation and loss of orientation and adhesion of immigrating fin mesenchyme cells. The gene encoding Slit3, an axon guidance ligand, is found to be mutated in stomp. Slit ligands derived from immigrating mesenchyme act via Robo receptors at the apical ectodermal ridge (AER) to promote the release of sphingosine-1-phosphate (S1P), which then diffuses back to the mesenchyme to regulate their polarization, orientation, positioning, and adhesion to the interstitial matrix of the fin fold.