Editorial Material
Cell & Tissue Engineering
Min-Chi Yang, Martha G. Rea-Moreno, Ya-Wen Chen
Summary: In a study published in Cell, Shiraishi et al.1 reveal the crucial role of mechanotransduction in maintaining lung epithelial cell fate, representing a significant milestone in understanding how mechanical factors regulate differentiation.
Article
Cell Biology
Tumpa Dutta, Nidhi Kapoor, Meril Mathew, Suban S. Chakraborty, Nathan P. Ward, Nicolas Prieto-Farigua, Aimee Falzone, James P. DeLany, Steven R. Smith, Paul M. Coen, Gina M. DeNicola, Stephen J. Gardell
Summary: The metabolic routing of Nicotinamide (NAM) to NAD+ or 1-methylnicotinamide (MeNAM) has significant implications for human health and aging. Through stable isotope tracing, it was found that 2H4-NAM acts as a precursor to NAD+ in cultured cells and human PBMCs, as well as in A549 cell xenografts and PBMCs from 2H4-NAM-dosed mice and humans. However, 2H4-NAM acts as a precursor to MeNAM only in A549 cell cultures and xenografts, not in isolated PBMCs. This research provides valuable insights into the regulation of NAD+ and MeNAM synthesis across different biological systems.
Article
Oncology
Hiba K. Omairi, Cameron J. Grisdale, Mathieu Meode, Alexandra K. Bohm, Sophie Black, Nancy J. Adam, Cassidy P. Chapman, Tatiana Maroilley, John J. Kelly, Maja Tarailo-Graovac, Steven J. M. Jones, Michael D. Blough, John Gregory Cairncross
Summary: This study reveals that PDGFA fails to induce the expression of kinetochore and spindle assembly checkpoint genes, leading to defective mitosis in neural progenitor cells (NPCs). Surviving cells with unstable genomes accumulate chromosomal rearrangements and give rise to tumorigenic NPCs with recurrent gains and losses of chromosomal regions. This work provides new insights into the mechanisms underlying the development of glioblastoma.
Article
Environmental Sciences
Min Zhang, Hailin Xia, Min Yu, Li Ju, Yun Xiao, Lijin Zhu
Summary: The study revealed that chrysotile caused dose-dependent DNA damage in MeT-5A and BEAS-2B cells, with MeT-5A cells showing more persistent damage. PARP1-deficient cells were found to be more sensitive to chrysotile-induced DNA damage compared to normal cells. Furthermore, the repair experiments showed that all cell lines were able to recover from the damage over time, indicating the important role of PARP1 in early repair of DNA damage in cells exposed to chrysotile.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Patrick J. Flynn, Peter D. Koch, Timothy J. Mitchison
Summary: Mitotic errors can activate cGAS and induce IFN signaling through the formation of chromatin bridges, which are selectively generated by microtubule stabilizers and MPS1 inhibitors. These chromatin bridges activate cGAS and lead to the transfer of 20 30-cGAMP between cells, suggesting a potential mechanism for immunosurveillance of cells that fail mitosis and antitumor actions of taxanes and MPS1 inhibitors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Pharmacology & Pharmacy
David M. Bernstein, Balazs Toth, Rick A. Rogers, Peter Kunzendorf, James Phillips, Dirk Schaudien
Summary: The study found that brake dust exposure caused significant differences in lung pathological responses and tumorigenicity compared to chrysotile asbestos and other amphiboles. While chrysotile showed short-term effects in the rat respiratory tract, crocidolite and amosite resulted in persistent inflammation and lung tumor response.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2021)
Article
Cell Biology
Marina Rodriguez-Munoz, Martina Serrat, David Soler, Anna Genesca, Teresa Anglada
Summary: Chromosome bridges in cancer cells are often formed and may break during mitosis, with a direct correlation between the distance between bridge kinetochores and bridge breakage. The mechanisms responsible for chromosome bridge breakage during mitosis may depend on the separation between the bridge kinetochores. Previous studies have suggested mechanical stress or biochemical digestion as possible causes of bridge breakage in interphase cells, suggesting a multifactorial model for bridge breakage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Jie Zhang, Yang Hong, Liang Wang, Weiheng Hu, Guangming Tian, Di Wu, Yang Wang, Ling Dai, Ziran Zhang, Yue Yang, Jian Fang
Summary: This study aimed to detect and describe the distribution characteristics of circulating tumor cells (CTCs) and circulating tumor-derived endothelial cells (CTECs) in patients with advanced lung cancer, and explore the correlation between CTC/CTEC subtypes and novel prognostic biomarkers. CTCs and CTECs derived from 52 patients with advanced lung cancer were identified using the subtraction enrichment-immunofluorescence in situ hybridization (SE-iFISH) system.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Jose Thaiparambil, Chandra S. Amara, Subrata Sen, Nagireddy Putluri, Randa El-Zein
Summary: The exposure to cigarette smoke can induce centrosome amplification and clustering in lung epithelial cells, which may contribute to lung carcinogenesis. The cyclin D2-mediated centrosome clustering pathway is critical for mitosis and its inhibition can lead to cell death.
Article
Biochemistry & Molecular Biology
Meenu Maan, Neha Jaiswal Agrawal, Jaya Padmanabhan, Christelle Colin Leitzinger, Yainyrette Rivera-Rivera, Harold Saavedra, Srikumar P. Chellappan
Summary: TBK1 plays a novel role in regulating the spindle assembly checkpoint (SAC) in breast and lung cancer cells by interacting with and phosphorylating Cdc20 and Cdh1. Inhibition of TBK1 increases SAC component levels and impairs mitotic progression, suggesting that targeting TBK1 along with other mitotic regulators may be effective in eliminating cancer cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Oncology
Yang Hong, Jiahui Si, Jie Zhang, Ying Xiong, Jianzhi Zhang, Peter Ping Lin, Jian Fang, Yue Yang, Chao Lv, Yuanyuan Ma
Summary: This study investigated the distribution and clinical significance of circulating aneuploid cells (CACs) in resectable non-small cell lung cancer (NSCLC) patients. The pre-surgery small cell size CACs, especially the triploid subtype, could be regarded as a potential prognostic biomarker for patients with resectable NSCLC.
FRONTIERS IN ONCOLOGY
(2021)
Article
Pharmacology & Pharmacy
Barbara Pinto, Pedro Novais, Ana C. Henriques, Juliana Carvalho-Tavares, Patricia M. A. Silva, Hassan Bousbaa
Summary: This study investigated the combination of the apoptosis activator Navitoclax and the antimitotic BI2536 to overcome slippage and enhance cell death in mitosis. The results showed that the combination significantly reduced slippage and accelerated cell death, confirming the potential of apoptosis potentiators in circumventing resistance to antimitotics. The synergy/additive effect observed in vitro warrants further clinical research for the BI2536/Navitoclax combination.
Article
Pharmacology & Pharmacy
Jomon Sebastian, Krishnan Rathinasamy
Summary: The study found that sertaconazole can bind to goat brain tubulin with higher affinity, blocking cell division during mitosis. Econazole and miconazole, on the other hand, may have additional targets and do not interfere with cell division during mitosis.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Minh Thi Tran, Chung Chinh Doan, Son Nghia Hoang, Cang Ngoc Ly, Mai Thi Phuong Nguyen, Quan Minh To, Nhung Hai Truong, Chi Nguyen Quynh Ho, Long Thanh Le
Summary: This research studied the impact of simulated microgravity on cell division in CCL-13 cells and found that simulated microgravity led to changes in cell cycle, nuclear/cytoplasmic ratio, and cell division structures.
APPLIED SCIENCES-BASEL
(2023)
Article
Biology
Bruno F. E. Matarese, Hassan Rahmoune, Nguyen T. K. Vo, Colin B. Seymour, Paul N. Schofield, Carmel Mothersill
Summary: This study characterizes the emission of acoustic waves from cultured cells exposed to X-ray photon radiation. Acoustic signals were recorded using an ultrasound transducer before, during, and after irradiation. The results show that radiation dose rate and cell viability affect the acoustic signal characteristics. Live and dead cells exhibit different acoustic signal patterns.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2023)
Article
Otorhinolaryngology
Jonatas Bussador do Amaral, Antonio Carlos Bloise, Carolina Nunes Franca, Claudina Perez-Novo, Glaucia Maria Machado-Santelli, Adriano Mesquita Alencar, Rogerio Pezato
AURIS NASUS LARYNX
(2020)
Article
Engineering, Biomedical
Beatriz F. M. Ribeiro, Marcelo M. Souza, Daniela S. Fernandes, Devaney R. do Carmo, Glaucia M. Machado-Santelli
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2020)
Article
Biochemistry & Molecular Biology
Sebastian O. Siri, Victoria Rozes-Salvador, Emilce Artur de la Villarmois, Marisa S. Ghersi, Gonzalo Quassollo, Mariela F. Perez, Cecilia Conde
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Bianca S. Takayasu, Igor R. Martins, Anali M. B. Garnique, Sayuri Miyamoto, Glaucia M. Machado-Santelli, Miriam Uemi, Janice Onuki
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2020)
Article
Cell Biology
Favio Pesaola, Gonzalo Quassollo, Ana Clara Venier, Ana Lucia De Paul, Ines Noher, Mariano Bisbal
Summary: The study found that CLN8 deficiency impacts the size of the Golgi apparatus, the number of mobile vesicles, and the speed of endo-lysosomes, resulting in lysosomal alkalization and affecting neuronal dendritic development. The results indicate that CLN8 plays a crucial role in vesicular distribution, lysosomal pH, and neuronal morphology development.
BIOLOGY OF THE CELL
(2021)
Article
Neurosciences
Guillermo Fernandez, Favio Krapacher, Soledad Ferreras, Gonzalo Quassollo, Macarena Mariel Mari, Maria Victoria Pisano, Antonella Montemerlo, Maria Dolores Rubianes, Claudia Bregonzio, Carlos Arias, Maria Gabriela Paglini
Summary: This study demonstrated that mice lacking the p35 protein exhibit symptoms of hyperactivity similar to ADHD, and have altered dopaminergic neurotransmission. The findings suggest a role of Cdk5/p35 in regulating DAT expression and function, providing insights into the physiology of dopamine neurotransmission and potential therapeutic options for dopamine-related pathologies such as ADHD.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Otorhinolaryngology
Carolina Nunes Franca, Andre Luis Lacerda Bachi, Eduardo Macoto Kosugi, Rogerio Pezato, Glaucia Maria Machado Santelli, Jonatas Bussador do Amaral
Summary: This study aimed to create a three-dimensional cell culture model for the study of nasal polyposis. The researchers successfully cultivated and analyzed spheroids by mechanically dissociating nasal polyp tissue from patients. The results showed that cells maintained differentiation and ciliary beating in a three-dimensional culture environment.
BRAZILIAN JOURNAL OF OTORHINOLARYNGOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jorge Henrique Neves, Paula Rezende-Teixeira, Natalia Bazan Palomino, Glaucia Maria Machado-Santelli
Summary: The study focused on molecular characterization and analysis of expression profile and cellular localization of innexin-2 in R. americana, revealing its role in various physiological processes during development. This research contributes to the understanding of invertebrate biology and gene regulation.
Article
Environmental Sciences
Bianca H. Ventura Fernandes, Natalia Martins Feitosa, Ana Paula Barbosa, Camila Gasque Bomfim, Anali M. B. Garnique, Ivana F. Rosa, Maira S. Rodrigues, Lucas B. Doretto, Daniel F. Costa, Bruno Camargo-dos-Santos, Gabrielli A. Franco, Joao Favero Neto, Juliana Sartori Lunardi, Marina Sanson Bellot, Nina Pacheco Capelini Alves, Camila C. Costa, Mayumi F. Aracati, Leticia F. Rodrigues, Rafaela Hemily Cirilo, Raul Marcelino Colagrande, Francisco I. F. Gomes, Rafael T. Nakajima, Marco A. A. Belo, Percilia Cardoso Giaquinto, Susana Luporini de Oliveira, Silas Fernandes Eto, Dayanne Carla Fernandes, Wilson G. Manrique, Gabriel Conde, Roberta R. C. Rosales, Iris Todeschini, Ilo Rivero, Edgar Llontop, German G. Sgro, Gabriel Umaji Oka, Natalia Fernanda Bueno, Fausto K. Ferraris, Mariana T. Q. de Magalhaes, Renata J. Medeiros, Juliana M. Mendonca-Gomes, Mara Souza Junqueira, Katia Conceicao, Leticia Gomes de Pontes, Antonio Condino-Neto, Andrea C. Perez, Leonardo J. G. Barcellos, Jose Dias Correa Junior, Erick Gustavo Dorlass, Niels O. S. Camara, Edison Luiz Durigon, Fernando Q. Cunha, Rafael H. Nobrega, Glaucia M. Machado-Santelli, Chuck S. Farah, Flavio P. Veras, Jorge Galindo-Villegas, Leticia Costa-Lotufo, Thiago M. Cunha, Roger Chammas, Luciani R. Carvalho, Cristiane R. Guzzo, Guilherme Malafaia, Ives Charlie-Silva
Summary: This study found that injection of SARS-CoV-2 Spike protein rSpike in zebrafish resulted in mortality and adverse effects on liver, kidney, ovary, and brain tissues, with a survival rate of 78.6%. The conserved genetic homology between zebrafish and humans may contribute to the intense toxic effects observed. The results highlight the potential of zebrafish as an animal model for translational COVID-19 research and for understanding ecotoxicological impacts, vaccine responses, and therapeutic approaches.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Ivana F. Rosa, Ana P. B. Pecanha, Tabata R. B. Carvalho, Leonardo S. Alexandre, Vinicius G. Ferreira, Lucas B. Doretto, Beatriz M. Souza, Rafael T. Nakajima, Patrick da Silva, Ana P. Barbosa, Leticia Gomes-de-Pontes, Camila G. Bomfim, Glaucia M. Machado-Santelli, Antonio Condino-Neto, Cristiane R. Guzzo, Jean P. S. Peron, Magaiver Andrade-Silva, Niels O. S. Camara, Anali M. B. Garnique, Renata J. Medeiros, Fausto K. Ferraris, Leonardo J. G. Barcellos, Jose D. Correia-Junior, Jorge Galindo-Villegas, Monica F. R. Machado, Angela Castoldi, Susana L. Oliveira, Camila C. Costa, Marco A. A. Belo, Giovane Galdino, German G. Sgro, Natalia F. Bueno, Silas F. Eto, Flavio P. Veras, Bianca H. V. Fernandes, Paulo R. S. Sanches, Eduardo M. Cilli, Guilherme Malafaia, Rafael H. Nobrega, Aguinaldo S. Garcez, Emanuel Carrilho, Ives Charlie-Silva
Summary: The study investigates the potential effect of red photobiomodulation (PBM) as a therapy for COVID-19. The findings suggest that inflammation is a significant feature of the disease, and PBM can help regulate the inflammatory response. Metabolomics analysis also reveals shared impacts on metabolic pathways between PBM and COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Keli Lima, Livia Bassani Lins de Miranda, Anali Del Milagro Bernabe Garnique, Bruna Oliveira de Almeida, Mariane Cristina do Nascimento, Guilherme Augusto Sousa Alcantara, Glaucia Maria Machado-Santelli, Eduardo Magalhaes Rego, Joao Agostinho Machado-Neto
Summary: AD80, a multikinase inhibitor, exhibits antineoplastic effects in pancreatic cancer cells by reducing cell viability and inducing mitotic aberrations, autophagy, and apoptosis. The drug also modulates several genes and signaling pathways, providing insights into potential therapeutic strategies for pancreatic cancer.
Article
Multidisciplinary Sciences
Bianca Sayuri Takayasu, Sheila Silva Rodrigues, Carlos Eduardo Madureira Trufen, Glaucia Maria Machado-Santelli, Janice Onuki
Summary: Snake envenomation is a neglected tropical disease, with Bothrops genus accounting for a large portion of snakebite accidents in Brazil. This study aimed to analyze the cytotoxicity and molecular mechanisms of Bothrops spp. venoms on rat liver cells. The results showed that the venoms decreased cell viability and disrupted microtubules and actin network, without causing cell cycle arrest. No clear correlation was found between the IC50 values and enzymatic/biological activities of the venoms. This work contributes to understanding and improving the treatment of snake envenomation.
Article
Biology
A. M. B. Garnique, P. Rezende -Teixeira, G. M. Santelli
Summary: TMPyP4 and TQ, as telomerase inhibitors, not only impact cellular proliferation and death, but also affect cell adhesion and cell cycle. They exhibit different effects on different types of cells.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2023)
Meeting Abstract
Cell Biology
K. Lima, L. B. L. de Miranda, A. D. B. Garnique, B. O. de Almeida, M. C. do Nascimento, G. M. Machado-Santelli, E. M. Rego, J. A. Machado-Neto
MOLECULAR BIOLOGY OF THE CELL
(2023)
Meeting Abstract
Cell Biology
K. Lima, L. B. L. De Miranda, A. D. B. Garnique, B. O. De Almeida, M. C. Do Nascimento, G. M. Machado-Santelli, E. M. Rego, J. A. Machado-Neto
MOLECULAR BIOLOGY OF THE CELL
(2023)