Article
Chemistry, Analytical
Chung-An Chen, Ping-Yeh Chiu, Tse-Hao Huang, Natalie Yi-Ju Ho, Fu-Cheng Kao, Tsung-Ting Tsai
Summary: Appropriate follow-up after treatment initiation in patients with osteoporosis is challenging. Serum biomarkers, such as procollagen type I propeptide (PINP), can provide clinically useful information for disease management. However, rapid and cost-effective methods for detecting serum PINP are lacking. In this study, we developed a high-sensitivity lateral flow immunoassay with a stacking pad (sLFIA) for quantitative detection of PINP and found that it can serve as a point-of-care test (POCT) for monitoring medication response and managing osteoporosis.
ANALYTICA CHIMICA ACTA
(2023)
Article
Nanoscience & Nanotechnology
Li Song, Chunmei Xie, Xueke Liu, Zhen Huo, Yinhai Xie, Jiafeng Gao, Shuping Zhou, Jing Shen, Xiaolong Tang, Xinkuang Liu
Summary: The study developed a chemiluminescence method for detecting serum intact PINP, a potential marker for osteoporosis. Results showed that compared with the Roche PINP assay, this method had higher sensitivity, wider linear range, and anti-interference capabilities.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2021)
Article
Medicine, General & Internal
Rihwa Choi, Sang Gon Lee, Eun Hee Lee
Summary: This study aimed to investigate intra-individual changes in total procollagen-type 1 N-terminal pro-peptide (P1NP) in a Korean adult population, providing insights into patient populations and test utilization. The findings showed that majority of patients experienced a decrease in P1NP during follow-up, while a smaller proportion had an increase. Furthermore, the type of medical institution did not significantly influence the change in P1NP during follow-up.
Article
Medicine, Research & Experimental
Yongchang Wu, Yishi Li, Yang Bai, Jinyue Jiang, Xiaohui Wang, Yi Chen, Xin Wang, Guichuan Huang, Yiling Gan, You Li, Shuliang Guo
Summary: This study focused on the clinical significance of serum TGF-beta 1 and PINP in post-tuberculosis tracheobronchial stenosis, showing that these markers may be potential tools for diagnosing and monitoring PTTS. The findings suggest that TGF-beta 1 and PINP levels are associated with airway stenosis characteristics and outcomes, with the potential to distinguish cases of stenosis from non-stenosis.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Andrology
Stefan Aufderklamm, Joerg Hennenlotter, Steffen Rausch, Cornelia Bock, Eva Erne, Christian Schwentner, Arnulf Stenzl
Summary: The study confirms that increased levels of 1CTP and P1NP concentrations are associated with the presence of metastatic disease in the bone, and can predict the clinical course in PC patients with bone metastases. The potential use of these markers for treatment selection in advanced PC needs further investigation.
TRANSLATIONAL ANDROLOGY AND UROLOGY
(2021)
Article
Cell Biology
Nicola L. Stevenson, Dylan J. M. Bergen, Yinhui Lu, M. Esther Prada-Sanchez, Karl E. Kadler, Chrissy L. Hammond, David J. Stephens
Summary: Knockout of the golgin giantin leads to skeletal and craniofacial defects due to changes in glycosylation and extracellular matrix deposition. Our study reveals a conserved role for giantin in collagen biosynthesis and extracellular matrix assembly, as well as a giantin-dependent pathway for intracellular procollagen processing.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Psychiatry
Ya-Mei Bai, Yu-Li Liu, Hsiang -Wei Kuo, Shih-Jen Tsai, Ju-Wei Hsu, Kai-Lin Huang, Pei-Chi Tu, Mu-Hong Chen
Summary: Higher levels of neurofilament light chain and proinflammatory cytokines were observed in patients with bipolar disorder and major depressive disorder. The bone turnover biomarker, P1NP, was related to MDD. The association among the brain-bone axis, systemic inflammation, and cognitive function remains unclear in severe affective disorders.
JOURNAL OF PSYCHIATRIC RESEARCH
(2023)
Article
Chemistry, Analytical
Shuang Han, Fang Gong, Yifeng Xue, Chunxin Wang, Xiaowei Qi
Summary: This study developed a chemiluminescence assay for P1NP and evaluated changes in bone metabolism markers in lung transplant patients. The results showed that P1NP and β-CrossLap are good clinical markers for monitoring bone mass.
JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY
(2022)
Article
Microbiology
Dane Granger, Nicholas T. Streck, Elitza S. Theel
Summary: This study suggests that a single antigen detection assay can be used to simultaneously detect Histoplasma and Blastomyces, with a high level of diagnostic accuracy. By utilizing the cross-reactivity between the two antigen detection assays, test utilization can be optimized and patient costs can be decreased.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Endocrinology & Metabolism
Sarah Seberg Diemar, Louise Lylloff, Maria Sode Ronne, Line Tang Mollehave, Malene Heidemann, Betina Heinsbaek Thuesen, Jesper Johannesen, Anders J. Schou, Steffen Husby, Niels Wedderkopp, Christian Molgaard, Niklas Rye Jorgensen
Summary: The study aimed to establish reference intervals for bone turnover markers in children and adolescents, showing that these markers vary with age and sex, with β-CTX and PINP being the most affected by these changes which reflect bone metabolism processes.
Article
Psychiatry
Henrik Maagensen, Julie R. Larsen, Niklas R. Jorgensen, Anders Fink-Jensen, Tina Vilsboll
Summary: The study found that treatment with liraglutide did not have any significant impact on bone turnover markers in patients with schizophrenia and prediabetes. Additionally, there was no association between changes in bone turnover markers and changes in body weight in the liraglutide-treated group. This suggests that liraglutide may not have adverse effects on bone metabolism in these patients.
PSYCHIATRY RESEARCH
(2021)
Article
Endocrinology & Metabolism
Masafumi Kashii, Takashi Kamatani, Yoshio Nagayama, Akira Miyama, Hideki Tsuboi, Kosuke Ebina
Summary: Baseline serum PINP value is significantly and independently associated with increased bone mineral density (≥ 3%) in both total hip and femoral necks after 12 months of romosozumab treatment in treatment-naive postmenopausal osteoporosis patients.
OSTEOPOROSIS INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Minjung Chae, Il-Hong Bae, Sunghwan Lim, Kyoungmi Jung, Jonghwa Roh, Wangi Kim
Summary: The study found that cortisol inhibits the expression of collagen type I, but AP collagen peptides or GR inhibitors can reverse this effect and prevent the cortisol-induced inhibition of the TGF-beta signaling pathway, ultimately improving skin health.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Public, Environmental & Occupational Health
G. M. Oremek, F. Holzgreve, C. Maurer-Grubinger, P. Diaremes, C. Erbe, D. Ohlendorf
Summary: This article provides an informative summary of the bone formation marker P1NP, which is highly sensitive and specific for monitoring treatment, progression, and recurrence of bone metastases. However, it is not suitable as a screening parameter for early diagnosis or prevention.
ZENTRALBLATT FUR ARBEITSMEDIZIN ARBEITSSCHUTZ UND ERGONOMIE
(2023)
Article
Biochemical Research Methods
Lun Bian, Yufeng Xiong, Hui Zhao, Huiling Guo, Zhaoyue Li, Ke Ye, Zhigao Zhang, Tiancai Liu, Yingsong Wu, Guanfeng Lin
Summary: In this study, a Eu (III) chelate nanoparticles based lateral flow immunoassay was developed to achieve real-time monitoring of Cys C concentration in serum within 15 min. This method showed a wide detection range and a low limit of detection, and exhibited satisfactory correlation with chemiluminescence immunoassay.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Chemistry, Analytical
Makiko Yoshida, Hinako Chida, Fukiko Kimura, Shohei Yamamura, Keiko Tawa
Article
Chemistry, Analytical
Hajime Shigeto, Eriko Yamada, Mizuki Kitamatsu, Takashi Ohtsuki, Akira Iizuka, Yasuto Akiyama, Shohei Yamamura
Article
Pharmacology & Pharmacy
Dai Majima, Ryosuke Mitsuhashi, Misaki Yamasaki, Kazuaki Kajimoto, Tatsuya Fukuta, Kentaro Kogure
Summary: The study demonstrated that α-tocopheryl succinate (TS) can suppress lipid accumulation in adipocytes by regulating lipid metabolic cell signaling, promoting lipolysis, and inhibiting lipid synthesis.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Chemistry, Analytical
Kazumichi Yokota, Muneaki Hashimoto, Kazuaki Kajimoto, Masato Tanaka, Sanae Murayama, Makusu Tsutsui, Yoshihiro Nakajima, Masateru Taniguchi, Masatoshi Kataoka
Summary: In this study, the resistive pulse method (RPM) was used to accurately discriminate cancer cells from leukocytes by measuring individual cell size, deformability, and surface charge in a solution with a low electrolyte concentration.
Editorial Material
Chemistry, Analytical
Shohei Yamamura
Article
Chemistry, Analytical
Kazumichi Yokota, Asae Takeo, Hiroko Abe, Yuji Kurokawa, Muneaki Hashimoto, Kazuaki Kajimoto, Masato Tanaka, Sanae Murayama, Yoshihiro Nakajima, Masateru Taniguchi, Masatoshi Kataoka
Summary: This study demonstrates the successful discrimination of different yeasts using the resistive pulse method, ensuring efficient production and safety. By measuring the ionic current response of cells and evaluating the shape of the current blockage waveform, accurate discrimination between Saccharomyces pastorianus and Dekkera spp. was achieved, along with quantitative detection of budding cells in Saccharomyces pastorianus.
Article
Chemistry, Medicinal
Kentaro Tabara, Kazunori Watanabe, Hajime Shigeto, Shohei Yamamura, Takamasa Kishi, Mizuki Kitamatsu, Takashi Ohtsuki
Summary: This study introduces a new double-stranded PNA/DNA probe for miR-221 detection, showing higher target sensitivity and promising applications for miRNA diagnosis.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Materials Science, Multidisciplinary
Jeladhara Sobhanan, Yuta Takano, Sakiko Sugino, Eri Hirata, Shohei Yamamura, Vasudevanpillai Biju
Summary: This study reports a method combining stem cell antigen (CD44)-specific immunosilica particles and immunofluorescent quantum dots for efficient detection and collection of cancer cells in blood samples. The approach allows for multimodal cancer cell detection and has potential applications in noninvasive cancer screening and postsurgical or therapeutic follow-up.
NPG ASIA MATERIALS
(2022)
Article
Chemistry, Analytical
Hidetaka Ueno, Yoshinori Akagi, Shohei Yamamura
Summary: In this study, a new cell patterning method using a light-responsive gas-generating polymer (LGP) and a conventional fluorescence microscope is proposed. The LGP allows for easy release of cells and tissues from the substrate surface using nitrogen gas bubbles generated by excitation light, without harming the cells. The method is simple, rapid, and highly accurate, and is not affected by the cell type or the ECM coating.
Article
Chemistry, Medicinal
Koki Ishii, Sakura Tsuchitani, Miyu Toyama, Hajime Shigeto, Shohei Yamamura, Takashi Ohtsuki, Yoshitane Imai, Mizuki Kitamatsu
Summary: We developed a method for detecting DNA using excimer fluorescence from PNA-Pyr probes, and optimized the spatial arrangement for optimal excimer formation.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2022)
Article
Engineering, Chemical
Hajime Shigeto, Takashi Ohtsuki, Shohei Yamamura, Mizuki Kitamatsu
Summary: In this study, the structure of peptide nucleic acid (PNA) molecular beacons as probes for detecting nucleic acids was adjusted. The fluorescence changes of PNA beacons with different numbers of Glu, Lys, and dabcyl (Dab) quenchers were investigated. The results showed that the number of Glu/Lys or Dab had an impact on the fluorescence changes, and the prehybrid of P1Q1 with Q-DNA was able to successfully detect DNA sequences.
Article
Biochemistry & Molecular Biology
Yosuke Tabei, Hiroko Abe, Shingo Suzuki, Nobuaki Takeda, Jun-ichiro Arai, Yoshihiro Nakajima
Summary: This study demonstrated that Sedanolide enhances cellular resistance to oxidative damage through the activation of the KEAP1-NRF2 pathway and upregulation of antioxidant enzymes. Additionally, Sedanolide attenuates cytosolic and mitochondrial ROS generation induced by H2O2, thereby exerting cytoprotective effects against H2O2-induced cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Hidetaka Ueno, Yoshinori Akagi, Shohei Yamamura
Summary: Selective cell retrieval is crucial for advancing cell analyzing technologies and regenerative medicine. This study presents a novel method using a light-responsive gas-generating polymer microarray for selective cell retrieval. The system allows easy and rapid retrieval of adhered cells without causing any damage.
Article
Microbiology
Muneaki Hashimoto, Kazumichi Yokota, Kazuaki Kajimoto, Musashi Matsumoto, Atsuro Tatsumi, Yoshihiro Nakajima, Toshihiro Mita, Noboru Minakawa, Hiroaki Oka, Masatoshi Kataoka