Article
Immunology
Jared Feldman, Julia Bals, Clara G. Altomare, Kerri St Denis, Evan C. Lam, Blake M. Hauser, Larance Ronsard, Maya Sangesland, Thalia Bracamonte Moreno, Vintus Okonkwo, Nathania Hartojo, Alejandro B. Balazs, Goran Bajic, Daniel Lingwood, Aaron G. Schmidt
Summary: Exposure to pathogens triggers adaptive immune responses; understanding the naive B cell repertoire aids in mounting protective responses; naive antibodies can recognize and neutralize SARS-CoV-2.
SCIENCE IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Evangelia Kokalaki, Biao Ma, Mathieu Ferrari, Thomas Grothier, Warren Hazelton, Somayya Manzoor, Eren Costu, Julia Taylor, Anna Bulek, Saket Srivastava, Isaac Gannon, Ram Jha, Rosalind Gealy, Lukas Stanczuk, Tatiana Rizou, Mathew Robson, Mohamed El-Kholy, Vania Baldan, Matteo Righi, James Sillibourne, Simon Thomas, Shimobi Onuoha, Shaun Cordoba, Martin Pule
Summary: CART cells recognizing CD19 effectively treat relapsed and refractory B-ALL and DLBCL, but CD19 loss often leads to relapse. Targeting a second antigen, CD22, can reduce antigen escape, but it is challenging due to low CD22 density and large structure. The characteristics and optimal strategy for CD22 CAR are not well-studied. Co-administration of CD19 and CD22 CARs is costly, and constructing single CARs targeting both antigens is difficult. A dual CART product, CAT/9A8 CART, is being tested in a phase I clinical study as a potential solution.
Article
Materials Science, Multidisciplinary
Ji Wang, Jianlong Chai, Hongpeng Zhang, Pengfei Tai, Chao Liu, Lijuan Niu, Wenhao He, Weiying Huang, Rui Shu, Laihui Luo, Weiping Li, Yabin Zhu, Cunfeng Yao, Peifeng Gao
Summary: This study evaluated Fe50Mn30Co10Cr10 dual-phase high-entropy alloy (DP-HEA) as a promising candidate material for future nuclear energy systems due to its stable twinning structures and high strength under displacement damage. The introduction of TWIP and TRIP mechanisms in the alloy improved both irradiation resistance and mechanical properties, with no elemental segregation detected after irradiation. Defects free channels and Lomer-Cottrell locks observed in irradiated samples contributed to sustaining dislocations and preventing dislocation pile-up along grain boundaries.
JOURNAL OF NUCLEAR MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Huijia Song, Feng Wang, Yayun Zhao, Ruixia Gao, Yulian He, Qing Yan, Xiaoyi Chen, Lisa D. Pfefferle, Silong Xu, Ying Sheng
Summary: This article proposes a method for simultaneous detection of dual biomarkers based on anti-interference magnetic molecularly imprinted polymers, which has been successfully applied in the detection of Parkinson's disease bio-indicators DOPAC and DA in human urine. The method exhibits good anti-interference, high accuracy, and fast batch detection.
Article
Chemistry, Applied
Rong Chai, Yuanyuan Wang, Xianwen Kan
Summary: In this study, a molecularly imprinted polymer-based electrochemical sensor was prepared for sensitive detection of ovalbumin (OVA) using boronate affinity. The dual-recognition elements in the sensor enabled efficient differentiation of OVA from other proteins. The sensor showed promising potential applications in clinical diagnosis.
Article
Biochemistry & Molecular Biology
Xiao Xu, Tong Zhang, Stefano Angioletti-Uberti, Yongqin Lv
Summary: This study investigates the interaction mechanism between copolymer nanoparticles and proteins through simulation and experimental analysis. It is found that copolymers with different charges and hydrophobicities have different effects on protein adsorption. A coverage-dependent model is proposed to explain the binding free energy between copolymers and proteins.
Article
Engineering, Electrical & Electronic
Yuxiang Wang, Jie Chu, Yu Chen, Dong Liang, Kailin Wen, Jueping Cai
Summary: Neural network-based computer vision is widely used in industrial image detection for fast and accurate defect recognition. In this study, a dual entropy-controlled convolutional neural network (DENC-CNN) combining feature entropy consistency (FEC) and gradient contribution entropy (GCE) is proposed to overcome the challenges in micro LED chip defect recognition. The proposed model achieves high accuracy, G-mean, and F1-score for defect recognition.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Article
Chemistry, Analytical
Yun Tan, Xiaohui Zhang, Weiyang Tang, Weiye Zhong, Jin Fan, Donghua Guo, Xiaolong Wu, Yizhen Liu
Summary: A high abundance sensitivity SNV detection strategy based on entropy-driven catalytic amplification adjusted by stoichiometry is proposed in this study, which can improve SNV identification specificity and enable high sensitivity detection of low abundance targets. The practical applicability of the adjusted EDC system was verified by p53 mutation discrimination in human urine.
ANALYTICA CHIMICA ACTA
(2021)
Article
Engineering, Environmental
Yizhong Shen, Xiang Gao, Yiyin Zhang, Huanhuan Chen, Yingwang Ye, Yongning Wu
Summary: Development of a simple and efficient dual-mode analytical technique with cross reference correction feature is important for accurate detection of target pollutants in the environment. The study describes the synthesis of a Fe-doped polydopamine-based nanozyme and the development of a dual-recognition strategy-driven fluorescence-colorimetric dual-mode detection platform for L. monocytogenes. The platform successfully detected L. monocytogenes in real environmental samples, demonstrating the potential of dual-recognition strategy for efficient and reliable analytical platforms.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Sha Yang, Jie Luo, Ligai Zhang, Liu Feng, Yuan He, Xueping Gao, Shuang Xie, Mingxuan Gao, Dan Luo, Kai Chang, Ming Chen
Summary: A smart nano-theranostic platform based on dual-miRNAs guided self-feedback DNA circuit is developed to detect and treat tumors. The platform can detect low abundance oncogenic miRNAs and visualize miRNAs inside cells, tissues, and mice. By triggering the release of therapeutic miRNAs through self-feedback, the platform demonstrates enhanced antitumor efficacy and biosafety.
Article
Biology
Abdellali Kelil, Eugenio Gallo, Sunandan Banerjee, Jarrett J. Adams, Sachdev S. Sidhu
Summary: Synthetic antibody technologies are efficient for generating monoclonal antibodies against human antigens, but they often rely on purified proteins excluding integral membrane proteins. CellectSeq is an antibody discovery strategy targeting integral membrane proteins, using in situ cell-based selections, next-generation sequencing, and motif-based scoring to identify antibodies with high diversities and specificities.
COMMUNICATIONS BIOLOGY
(2021)
Article
Chemistry, Analytical
Yong Chang, Yixuan Chen, Mian Wu, Lin Liu, Qijun Song
Summary: In this study, a sandwich-type electrochemical aptasensor was developed using 4-formylphenylboric acid (FPBA)-modified Cu-based metal-organic frameworks (FPBA-Cu-MOFs) as dual-functional signal probes for glycoprotein detection. The aptamer-modified electrode captured the target, allowing the attachment of FPBA-Cu-MOFs based on the interaction between boronic acid and glycan on glycoproteins. FPBA-Cu-MOFs produced an amplified signal for the direct voltammetric detection of glycoproteins, achieving a detection limit as low as 6.5 pg mL-1 for prostate specific antigen detection. This method eliminates the use of antibodies and enzymes for molecular recognition and signal output, and can be applied to the design of other biosensors for the determination of diol-containing biomolecules in clinical diagnosis.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Yong Chang, Yixuan Chen, Mian Wu, Lin Liu, Qijun Song
Summary: In this study, a sandwich-type electrochemical aptasensor was developed using 4-formylphenylboric acid (FPBA)-modified Cu-based metal-organic frameworks (FPBA-Cu-MOFs) as dual-functional signal probes for the detection of glycoproteins. The aptamer-modified electrode captured the glycoprotein target, allowing the attachment of FPBA-Cu-MOFs through boronic acid-glycan interactions. The amplified signal from the abundant Cu2+ ions in FPBA-Cu-MOFs enabled the direct voltammetric detection of glycoproteins, achieving a low detection limit of 6.5 pg mL-1 for prostate specific antigen.
ANALYTICAL METHODS
(2023)
Article
Immunology
Gabrielle Warner Jenkins, Yana Safonova, Vaughn V. Smider
Summary: Ultralong third H chain complementarity-determining regions (CDR H3) are unique to bovine antibodies, allowing them to bind to difficult epitopes. Diversity in the knob domain of CDR H3 is formed through a combination of germline polymorphisms, deletions, and somatic hypermutation, resulting in a complex array of cysteine content and disulfide bond patterns.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Songhai Tian, Xiaozhe Xiong, Ji Zeng, Siyu Wang, Benjamin Jean-Marie Tremblay, Peng Chen, Baohua Chen, Min Liu, Pengsheng Chen, Kuanwei Sheng, Daniel Zeve, Wanshu Qi, David T. Breault, Cesar Rodriguez, Ralf Gerhard, Rongsheng Jin, Andrew C. Doxey, Min Dong
Summary: Toxin B (TcdB) is a major exotoxin responsible for diseases associated with Clostridioides difficile infection. This study identifies tissue factor pathway inhibitor (TFPI) as a host receptor for TcdB4 and TcdB10 subtypes, and reveals intragenic micro-recombination (IR)-driven changes on receptor specificity among TcdB variants.
NATURE COMMUNICATIONS
(2022)