Article
Multidisciplinary Sciences
Li-Ting Wang, Abira Rajah, Claire M. Brown, Luke McCaffrey
Summary: The short intracellular domain of CD13 protein is essential for capturing endosomes at the apical site and is crucial for cell polarization.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Carina Magdaleno, Trenton House, Jogendra S. Pawar, Sophia Carvalho, Narendiran Rajasekaran, Archana Varadaraj
Summary: This study explores the impact of fibronectin (FN) matrix on epithelial functions in the context of cancer progression, showing that FN fibril formation decreases during hypoxia, leading to reduced migratory potential. Additionally, disruption of FN fibrils in normal breast cells results in acinar luminal filling, indicating a loss of apicobasal polarity.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Biology
Yun Qi, Han Liu, Kang Zhang, Yihui Wu, Chenghao Shen, Xinhua Lin
Summary: This study reveals that Ihog proteins play crucial roles in integrin-mediated adhesions and regulate adhesion by interacting with integrins.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Article
Cell Biology
Arun A. Chandrakumar, Etienne Coyaud, Christopher B. Marshall, Mitsuhiko Ikura, Brian Raught, Robert Rottapel
Summary: Rab11 GTPase proteins play important roles in cytokinesis, ciliogenesis, and lumenogenesis; SH3BP5 and SH3BP5L are crucial for the activation of Rab11a and cyst lumen formation; Tankyrase regulates epithelial polarity and lumen formation by inhibiting SH3BP5 and SH3BP5L, which is counteracted by RNF146.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Developmental Biology
Shuyu Liu, Dawei Sun, Richard Butler, Emma L. Rawlins
Summary: This study expands multipotent epithelial progenitor cells from human embryonic lungs as organoids and maintains their self-renewing state. The cells exhibit columnar shape, resembling the developing lung tip epithelium. The study identifies the signaling pathways, including FGF7 and integrin signaling, that regulate cell shape and development in human lung progenitors.
Article
Biology
Maria Danielle Sallee, Melissa A. Pickett, Jessica L. Feldman
Summary: Research on developing Caenorhabditis elegans intestines reveals the importance of the apical PAR complex in maintaining intestinal epithelial integrity and constructing a functional tube. Depletion of PAR-6, PKC-3, and CDC-42 regulators leads to gaps in apical proteins, hindering food passage and causing larval death.
Article
Cell Biology
Ioanna Pitsidianaki, Jason Morgan, Jamie Adams, Kyra Campbell
Summary: The study reveals that Mesenchymal-to-epithelial transition (MET) during midgut morphogenesis in Drosophila melanogaster involves down-regulation of the EMT transcription factor Serpent, secretion of specific laminins via the CopII secretory pathway, and basal polarization of alpha PS1 integrin. These mechanisms are crucial for the formation and positioning of the midgut epithelium, and targeting these pathways along with therapies preventing EMT may provide a strategy to block metastasis in cancer.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Physics, Multidisciplinary
Florian Muckel, Stephan von Malottki, Christian Holl, Benjamin Pestka, Marco Pratzer, Pavel F. Bessarab, Stefan Heinze, Markus Morgenstern
Summary: Magnetic skyrmions have potential applications in memory, logic, and neuromorphic computing due to their topological protection caused by swirling spin textures. Understanding the microscopic mechanism of unwinding is crucial for enhancing the stability of skyrmions.
Article
Multidisciplinary Sciences
Georgii Pobegalov, Lee-Ya Chu, Jan-Michael Peters, Maxim I. Molodtsov
Summary: The spatial organization of DNA is facilitated by cohesin protein complexes, which move on DNA and extrude DNA loops. However, the mechanistic functioning of cohesin as a molecular machine is not well understood. In this study, we measured the mechanical forces generated by conformational changes in single cohesin molecules and found that bending of SMC coiled coils is driven by random thermal fluctuations, while ATPase head engagement occurs through an ATP dependent head-head movement. Our findings shed light on the force-generating mechanisms of cohesin and provide insights into the cohesin-DNA interaction.
NATURE COMMUNICATIONS
(2023)
Article
Hematology
Caitlin R. Francis, Shea Claflin, Erich J. Kushner
Summary: Slp2a is essential for vascular lumen formation by regulating WPB exocytosis and maintaining the release of Angiopoietin-2. Its absence disrupts the fusion of WPB with the apical membrane, blocking Tie-2 signaling necessary for proper lumen formation. WPB-housed Angiopoietin-2 is identified as a crucial factor in vascular lumenization.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Jifeng Liu, Hongyan Long, Dagmar Zeuschner, Andreas F. B. Rader, William J. Polacheck, Horst Kessler, Lydia Sorokin, Britta Trappmann
Summary: Current tissue engineering strategies lack materials that promote angiogenesis. Here the authors develop a microfluidic in vitro model in which chemokine-guided endothelial cell sprouting into a tunable hydrogel is followed by the formation of perfusable lumens to determine the material properties that regulate angiogenesis.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Sayema Rahman, Norman van Rhijn, Panagiotis Papastamoulis, Darren D. D. Thomson, Zorana Carter, Rachael Fortune-Grant, Magnus Rattray, Michael John Bromley, Elaine Bignell
Summary: This study provides a comprehensive overview of the transcriptional regulatory genes of A. fumigatus that govern lung epithelial damage, indicating highly coordinated genetic orchestration of host-damaging activities in both space and time.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Cell Biology
Daisuke Okuno, Noriho Sakamoto, Yoshiko Akiyama, Takatomo Tokito, Atsuko Hara, Takashi Kido, Hiroshi Ishimoto, Yuji Ishimatsu, Mohammed S. O. Tagod, Haruki Okamura, Yoshimasa Tanaka, Hiroshi Mukae
Summary: This study demonstrates that human gamma delta T cells can reduce collagen levels in lung fibroblasts through two distinct mechanisms, suggesting that adoptive gamma delta T cell transfer may be a potential new therapeutic approach.
Article
Peripheral Vascular Disease
Ben Ma, Elaina Melton, Robert Wiener, Ning Zhou, Wenqian Wu, Lo Lai, Charles Wang, Kevin D. Costa, Hongyu Qiu
Summary: The study found that aging-induced aortic stiffening is more prominent in hypertensive rats compared to normotensive rats. Hypertension-induced high blood pressure leads to more significant aortic stiffening in older normotensive rats. The combination of aging and hypertension has additive effects on aortic stiffness.
Article
Chemistry, Multidisciplinary
Shinji Kishimoto, Yuya Matsubara, Kenji Watanabe
Summary: Ergopeptines are representative ergoline alkaloids with a tripeptide extension on the lysergic acid core. In this study, we characterized newly isolated ergopeptine-like compounds called lentopeptins from Aspergillus lentulus, which differ from traditional ergopeptines in structure and biosynthetic pathway. Targeted deletion of len cluster biosynthetic genes predicted from the genome sequence revealed the atypical biosynthetic pathway of lentopeptins.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Cell Biology
Irina Raykhel, Fazeh Moafi, Satu M. Myllymaki, Patricia G. Greciano, Karl S. Matlin, Jose V. Moyano, Aki Manninen, Johanna Myllyharju
JOURNAL OF CELL SCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Teija Paakkola, Kari Salokas, Ilkka Miinalainen, Sin Lehtonen, Aki Manninen, Mika Kaakinen, Lloyd W. Ruddock, Markku Varjosalo, Riitta Kaarteenaho, Johanna Uusimaa, Reetta Hinttala
HUMAN MOLECULAR GENETICS
(2018)
Article
Optics
Tomi Pitkaaho, Aki Manninen, Thomas J. Naughton
Article
Biochemical Research Methods
Satu-Marja Myllymaki, Ulla-Reetta Kamarainen, Liu Xiaonan, Sara Pereira Cruz, Sini Miettinen, Mikko Vuorela, Markku Varjosalo, Aki Manninen
MOLECULAR & CELLULAR PROTEOMICS
(2019)
Article
Oncology
Nina Kozlova, Daniela Mennerich, Anatoly Samoylenko, Elitsa Y. Dimova, Peppi Koivunen, Ekaterina Biterova, Kati Richter, Antti Hassinen, Sakari Kellokumpu, Aki Manninen, Ilkka Miinalainen, Virpi Glumoff, Lloyd Ruddock, Lyudmyla Borysivna Drobot, Thomas Kietzmann
Article
Chemistry, Physical
Sry D. Hujaya, Aki Manninen, Kirsten Kling, Jakob B. Wagner, Seppo J. Vainio, Henrikki Liimatainen
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2019)
Review
Cell Biology
Satu-Marja Myllymaki, Marja L. Mikkola
CURRENT OPINION IN CELL BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Tomasz Wenta, Anette Schmidt, Qin Zhang, Raman Devarajan, Prateek Singh, Xiayun Yang, Anne Ahtikoski, Markku Vaarala, Gong-Hong Wei, Aki Manninen
Summary: This study investigated the role of PTEN and hemidesmosomal adhesions in prostate cancer and found that their simultaneous loss induces various tumorigenic properties, including proliferation, migration, resistance to anoikis, apoptosis, and increased metastatic capacity. These effects are dependent on plectin and are mediated by the activation of EGFR/PI3K/Akt and FAK/Src pathways.
Article
Cell Biology
Anette Schmidt, Mika Kaakinen, Tomasz Wenta, Aki Manninen
Summary: The loss of alpha 6 beta 4-mediated hemidesmosomes promotes cell migration and focal adhesion assembly dynamics by influencing the molecular diffusion rates of focal adhesion kinase (FAK).
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Yunxian Huang, Jinghua Gui, Satu-Marja Myllymaki, Kallol Roy, Tambet Tonissoo, Marja L. Mikkola, Osamu Shimmi
Summary: Epithelial homeostasis and growth are regulated by physical and biochemical signals from neighboring cells and tissues. In mammalian cells, the interaction between Scribble and alpha-Catenin regulates epithelial homeostasis by controlling the nuclear translocation of YAP in the Hippo signaling pathway. Studies using the Drosophila wing imaginal disc show that Scribble expression impacts epithelial growth, and ectopic expression of Scribble suppresses the YAP signal and leads to smaller wing size.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yosuke Senju, Toiba Mushtaq, Helena Vihinen, Aki Manninen, Juha Saarikangas, Katharina Ven, Ulrike Engel, Markku Varjosalo, Eija Jokitalo, Pekka Lappalainen
Summary: The MTSS1 protein binds to the Arp2/3 and WAVE-2 protein complexes, promoting the formation of dynamic membrane folds at cell-cell junctions and enhancing the integrity of these junctions.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Yunxian Huang, Jinghua Gui, Satu-Marja Myllymaki, Marja L. Mikkola, Osamu Shimmi
Summary: This study investigates the molecular mechanisms underlying apicobasal polarity-mediated growth control in the Drosophila wing imaginal disc. The findings reveal the key role of Scribble, Septate junction complex, and alpha-Catenin in maintaining apicobasal polarity and regulating tissue growth.
Article
Cell Biology
Satu-Marja Myllymaki, Beata Kaczynska, Qiang Lan, Marja L. Mikkola
Summary: Branching morphogenesis is an evolutionary strategy for enhancing epithelial function in compact organs. However, the coordination between cell elongation and branching remains unclear. In the embryonic mammary gland, live imaging revealed that tip cells advance through directional migration while elongation depends on differential cell motility, with lagging cells being fed into the trailing duct and supported by tip proliferation. Branching involves localized repression of cell cycle and motility, and daughter cells change direction to elongate new branches while remaining proliferative. Additionally, epithelial cell contractility is crucial for mammary branching morphogenesis.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Cell Biology
Ewelina Trela, Qiang Lan, Satu-Marja Myllymaki, Clementine Villeneuve, Riitta Lindstrom, Vinod Kumar, Sara A. Wickstrom, Marja L. Mikkola
Summary: The early growth of the mammary rudiment is primarily accomplished by migration-driven cell influx, with minor contributions of cell hypertrophy and proliferation. Additionally, an invagination mechanism of the mammary bud is driven by thin, elongated keratinocytes-ring cells forming a contractile rim, likely exerting force via the actomyosin network. Additionally, conditional deletion of nonmuscle myosin IIA impairs invagination, resulting in abnormal mammary bud shape.
JOURNAL OF CELL BIOLOGY
(2021)