Article
Multidisciplinary Sciences
Yang Han, Lin Zhong, Fuli Ren
Summary: This study established a simple and broad-spectrum system for preparing phosphorylation-positive samples, which were then validated in cells from different species and tissues, demonstrating effectiveness in evaluating the quality of phosphorylation-specific antibodies.
Article
Multidisciplinary Sciences
Yang Han, Lin Zhong, Fuli Ren
Summary: This study established a simple and broad-spectrum system for evaluating the quality of phosphorylation-specific antibodies, which was validated in multiple experiments and shown to be effective and applicable in various biomedical fields.
Article
Biochemistry & Molecular Biology
Zhi-Da Zhang, Bing Wen, Da-Jia Li, Dan-Xia Deng, Xiao-Dong Wu, Yin-Wei Cheng, Lian-Di Liao, Lin Long, Geng Dong, Li-Yan Xu, En-Min Li
Summary: In this study, we identified a novel phosphorylation site in Fascin and confirmed AKT2 as the kinase for this site. Phosphorylation of Fascin Threonine 403 suppresses the behavior of esophageal cancer cells, actin-bundling activity, and filopodia formation.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Article
Biology
Holly Fowle, Ziran Zhao, Qifang Xu, Jason S. Wasserman, Xinru Wang, Mary Adeyemi, Felicity Feiser, Alison N. Kurimchak, Diba Atar, Brennan C. McEwan, Arminja N. Kettenbach, Rebecca Page, Wolfgang Peti, Roland L. Dunbrack, Xavier Grana
Summary: The study elucidates the mechanism by which PP2A/B55 alpha recognizes substrates and dephosphorylates them, identifying a key binding site in p107 and validating the necessity of related SLiM motif sequences for substrate binding.
Article
Biochemistry & Molecular Biology
Weiwei Ke, Linlin Zhang, Xiangxuan Zhao, Zaiming Lu
Summary: The study found that the p53-m6A axis plays a crucial role in regulating the sensitivity of hepatocellular carcinoma to chemotherapy, highlighting the potential of targeting this axis in HCC therapy. By reducing METTL3 expression and increasing p53 expression, chemotherapy efficacy can be enhanced, providing a new insight into HCC treatment.
Article
Multidisciplinary Sciences
Andras Zeke, Tamas Takacs, Peter Sok, Krisztina Nemeth, Klara Kirsch, Peter Egri, Adam Levente Poti, Isabel Bento, Gabor E. Tusnady, Attila Remenyi
Summary: This study investigates the structural details of the control of SHP2 phosphatase activity through serine/threonine phosphorylation sites. The research reveals a direct link between these phosphorylation sites and the regulation of specific phosphotyrosine phosphatases, indicating a complex regulatory mechanism for SHP2.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Nadine Prust, Saar van der Laarse, Henk van den Toorn, Nina M. van Sorge, Simone Lemeer
Summary: This study utilized an optimized protocol for efficient phosphopeptide enrichment and LC-MS/MS to investigate protein phosphorylation in Staphylococcus aureus, resulting in a comprehensive phosphoproteome dataset that sheds light on signaling pathways in bacteria. The research significantly increased the number of phosphosite identifications compared to previous studies and identified downstream targets of a key Ser/Thr kinase in S. aureus, indicating a greater abundance of Ser/Thr kinase signaling in this bacterium.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
A. A. Zorina, G. V. Novikova, N. B. Gusev, A. V. Leusenko, D. A. Los, O. I. Klychnikov
Summary: Twelve potential serine-threonine protein kinases (STPKs) in Synechocystis sp. PCC 6803 genome were divided into two clusters: PKN2-type kinases and ABC1-type kinases. This study demonstrated that SpkH of Synechocystis is a true active serine protein kinase with substrate specificity similar to casein kinases and sensitivity to certain activity effectors.
Article
Biochemistry & Molecular Biology
Dhirendra K. Sharma, Subhash C. Bihani, Mohammad Q. Siddiqui, Hari S. Misra, Yogendra S. Rajpurohit
Summary: RqkA is a DNA damage responsive serine/threonine kinase with a unique structure in D. radiodurans. The WD40 domain in RqkA plays a crucial role in regulating its kinase signaling functions, interaction, and activation of kinase activity.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Immunology
Laurence Don Wai Luu, Ling Zhong, Sandeep Kaur, Mark J. Raftery, Ruiting Lan
Summary: The Bordetella genus is divided into classical and non-classical groups. Phosphorylation plays a key role in regulating metabolism and virulence pathways in Bordetella. The phosphorylation of key virulence regulator BvgA may be important in fine-tuning virulence gene expression and distinguishing classical from non-classical Bordetella species.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Neurosciences
Zhanmin Lin, Bin Wu, Maarten W. Paul, Ka Wan Li, Yao Yao, Ihor Smal, Martina Proietti Onori, Hana Hasanbegovic, Karel Bezstarosti, Jeroen Demmers, Adriaan B. Houtsmuller, Erik Meijering, Freek E. Hoebeek, Martijn Schonewille, August B. Smit, Zhenyu Gao, Chris De Zeeuw
Summary: PP2B controls synaptic function and learning through its action as a phosphatase and as a structural protein facilitating synapse integrity. In the synapses of cerebellar Purkinje cells, PP2B interacts with structural proteins, playing a crucial role in cerebellar learning.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biochemical Research Methods
Transito Garcia-Garcia, Thibaut Douche, Quentin Giai Gianetto, Sandrine Poncet, Nesrine El Omrani, Wiep Klaas Smits, Elodie Cuenot, Mariette Matondo, Isabelle Martin-Verstraete
Summary: This study provides a detailed map of kinase-substrate relationships in C. difficile, revealing the significant role of protein phosphorylation in bacterial cell signaling and pathogenicity. The identified phosphoproteins targeted different pathways involved in bacterial growth and metabolism, shedding light on their regulatory mechanisms.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Oncology
Huanxin Sun, Xiaowen Liu, Laicheng Wang, Bin Cui, Wenli Mu, Yu Xia, Shuang Liu, Xin Liu, Yulian Jiao, Yueran Zhao
Summary: In this study, the researchers demonstrated that upregulation of FKBP51 in acute monocytic leukemia cells decelerates proliferation and improves cytarabine sensitivity by inhibiting AKT pathways. Furthermore, the combination therapy of dexamethasone and Ara-C enhances the chemosensitivity of AML-M5.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
David Machover, Emma Goldschmidt, Wathek Almohamad, Vincent Castagne, Julien Dairou, Christophe Desterke, Lea Gomez, Yann Gaston-Mathe, Claude Boucheix
Summary: High-dose vitamin B6 enhances the antitumor potency of regimens comprising FUra and FA, as shown in this pilot study on breast cancer patients.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Yingying Gao, Bin Ma, Yifan Li, Xiangyu Wu, Shifeng Zhao, Huiping Guo, Yiwei Wang, Lihua Sun, Jing Xie
Summary: The study shows that Haspin is essential for maintaining a balanced ratio of asymmetric cell division (ACD) in mouse embryonic stem cells (mESCs). Knockout of Haspin leads to an increased ratio of ACD. This imbalance is rescued by overexpression of Wnt5a, suggesting that Haspin regulates ACD through the Wnt5a pathway.
CELL DEATH DISCOVERY
(2023)