Article
Biochemistry & Molecular Biology
Guido Papa, Anna Albecka, Donna Mallery, Marina Vaysburd, Nadine Renner, Leo C. James
Summary: HIV-1 utilizes IP6 to form a stable capsid for delivering its genome into host nucleus. Viruses unable to incorporate IP6 are not protected by capsid and are detected by innate immunity, leading to the activation of an antiviral state that inhibits infection.
Article
Immunology
Shiming Jiao, Nie Tan, Chengyu Zhu, Yan Ding, Wenyue Xu
Summary: Complement plays a crucial role in the immune response to bacterial and viral infections, but its role in malaria liver stage development remains unclear. In experiments, it was found that sporozoite infection activates systemic complement, but depletion or knockout of C3 does not significantly affect malaria liver stage development. However, incubation of serum with trypsin-treated sporozoites leads to the deposition of membrane attack complex (MAC) and reduces the number of exo-erythrocytic forms (EEF). Further studies suggest that complement H factor (CFH) may prevent MAC deposition on the surface of naive sporozoites.
PARASITE IMMUNOLOGY
(2023)
Article
Immunology
Americo Harry Lopez-Yglesias, Chun-Chi Lu, Marvin A. Lai, Ellen K. Quarles, Xiaodan Zhao, Adeline M. Hajjar, Kelly D. Smith
Summary: Salmonella enterica serovar Typhimurium is a major cause of worldwide gastroenteritis and poses a significant threat to vulnerable populations. Our study reveals that NLRC4 inflammasome induced innate immune responses play distinct roles in systemic and mucosal detection of flagellin and non-flagellin molecules during Salmonella infection.
INFECTION AND IMMUNITY
(2023)
Article
Virology
Li Gao, Rui Liu, Fuchun Yang, Xiaohan Li, Changjun Liu, Xiaole Qi, Hongyu Cui, Yanping Zhang, Suyan Wang, Xiaomei Wang, Yulong Gao, Kai Li
Summary: Duck enteritis virus (DEV) encodes multiple viral proteins to inhibit the cGAS-STING DNA-sensing pathway. The viral tegument protein UL41 selectively reduces the mRNA accumulation of interferon regulatory factor 7 (IRF7), thereby inhibiting the DNA-sensing pathway. Loss of UL41 enhances the immune response to DEV and impairs viral replication.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Dongshu Wang, Gang Chen, Yufei Lyu, Erling Feng, Li Zhu, Chao Pan, Weicai Zhang, Xiankai Liu, Hengliang Wang
Summary: In this study, a complete detection system for Bacillus anthracis based on Cas12a was developed. The system can detect the bacteria with high accuracy in 90 minutes and has single-copy level sensitivity. Additionally, a visualization system for field-based point-of-care detection was created, showing promising prospects.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Virology
Mijia Lu, Miaoge Xue, Hai-Tao Wang, Elizabeth L. Kairis, Sadeem Ahmad, Jiangbo Wei, Zijie Zhang, Qinzhe Liu, Yuexiu Zhang, Youling Gao, Dominique Garcin, Mark E. Peeples, Amit Sharma, Sun Hur, Chuan He, Jianrong Li
Summary: Viral m(6)A methylation is a common strategy in NNS RNA viruses to evade host innate immunity by serving as a molecular marker to discriminate self from nonself RNA. This novel biological function is universally conserved in several families within NNS RNA viruses.
JOURNAL OF VIROLOGY
(2021)
Article
Endocrinology & Metabolism
Hans-Georg Sprenger, Thomas MacVicar, Amir Bahat, Kai Uwe Fiedler, Steffen Hermans, Denise Ehrentraut, Katharina Ried, Dusanka Milenkovic, Nina Bonekamp, Nils-Goeran Larsson, Hendrik Nolte, Patrick Giavalisco, Thomas Langer
Summary: The study reveals that cytosolic pyrimidine levels regulate the release of mitochondrial DNA and activation of cGAS-STING.
Article
Fisheries
Fangshu Li, Wenjuan Liu, Jiwen Chen, Baoyu Huang, Yanxin Zheng, Jilv Ma, Shuai Cai, Lingling Li, Fengchen Liu, Xiaona Wang, Lei Wei, Yaqiong Liu, Meiwei Zhang, Yijing Han, Xuekai Zhang, Xiaotong Wang
Summary: In this study, a coding sequence of an interferon regulatory factor (IRF) gene, named CfIRF8-like, was obtained from the Zhikong scallop. The analysis revealed that CfIRF8-like has important signal transduction and immune functions in scallops and can interact with the protein CfIKK2. Overexpression of CfIRF8-like in human 293T cells activated the interferon beta promoter and interferon-stimulated response element promoter. These findings provide theoretical and experimental support for understanding the innate immune function of invertebrate IRFs and the development of comparative immunology.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Microbiology
Wei Wu, Yang Qu, Shengqing Yu, Sa Wang, Yuncong Yin, Qinfang Liu, Chunchun Meng, Ying Liao, Zaib Ur Rehman, Lei Tan, Cuiping Song, Xusheng Qiu, Weiwei Liu, Chan Ding, Yingjie Sun
Summary: DDX21 is identified as a key regulator of immune balance, with virus infection and RNA/DNA ligands leading to its cleavage and suppression of the interferon beta signaling pathway. Modulating DDX21 cleavage may impact the innate immune response to viruses.
Article
Biochemistry & Molecular Biology
Takahisa Kouwaki, Tasuku Nishimura, Guanming Wang, Reiko Nakagawa, Hiroyuki Oshiumi
Summary: Post-translational modification of LGP2 fine-tunes antiviral innate immune responses by promoting late-phase expression of type I interferons, while attenuating other antiviral proteins.
Review
Biochemistry & Molecular Biology
Carla Cacciotto, Alberto Alberti
Summary: Neutrophils play a crucial role in the immune response against infection, but some microorganisms can evade their attacks. Mycoplasma, a reduced genome bacteria, induces the release of neutrophil extracellular traps (NETs), but can digest the DNA scaffold of NETs to escape entrapment and promote infection. This persistence of mycoplasma infection and prolonged NET release are associated with the onset of chronic inflammatory diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Soumita Dutta, Ileana D. Corsi, Naomi Bier, Theresa M. Koehler
Summary: Research has shown that branched-chain amino acid synthesis is not sufficient for the robust growth of Bacillus anthracis; access to branched-chain amino acids is necessary for the proliferation of the pathogen in culture and during infection in a murine model. B. anthracis produces a large repertoire of branched-chain amino acid-related transporters, with three isoleucine/valine transporters identified with partial functional redundancy. Deletion of one of these transporters, BrnQ3, led to attenuated virulence. A mutant deficient in branched-chain amino acid biosynthesis grew well in medium containing these amino acids, but was also attenuated in virulence. These findings suggest that branched-chain amino acids are limited in multiple niches during infection and contribute to understanding the nutritional requirements of this important pathogen.
Article
Biochemistry & Molecular Biology
Asif J. Iqbal, Franziska Krautter, Isobel A. Blacksell, Rachael D. Wright, Shani N. Austin-Williams, Mathieu-Benoit Voisin, Mohammed T. Hussain, Hannah L. Law, Toshiro Niki, Mitsuomi Hirashima, Michele Bombardieri, Costantino Pitzalis, Alok Tiwari, Gerard B. Nash, Lucy Norling, Dianne Cooper
Summary: This study investigates the role of immunomodulatory lectin Galectin-9 (Gal-9) in neutrophil recruitment. The results show that Gal-9 is upregulated in inflamed blood vessels and released by activated endothelial cells. Gal-9 binding induces neutrophil activation and can enhance their interaction with endothelial cells. Both soluble and immobilized Gal-9 function as adhesion molecules and can capture neutrophils. These findings provide insights into the importance of Gal-9 in leukocyte recruitment during inflammation.
Article
Immunology
Joe James, Stephanie M. Meyer, Huynh A. Hong, Chau Dang, Ho T. Y. Linh, William Ferreira, Paidamoyo M. Katsande, Linh Vo, Daniel Hynes, William Love, Ashley C. Banyard, Simon M. Cutting
Summary: This study found that nasal dosing using heat-stable spores can reduce clinical symptoms of influenza infection and decrease viral shedding in animals. This approach may have important implications for influenza prophylaxis in both humans and animals.
Article
Immunology
Lingling Li, Wenjuan Liu, Nini Fan, Fangshu Li, Baoyu Huang, Qian Liu, Xiaomei Wang, Yanxin Zheng, Xiuxiu Sang, Juan Dong, Xiaona Wang, Lei Wei, Yaqiong Liu, Meiwei Zhang, Jilv Ma, Jiwen Chen, Yitao Qi, Xiaotong Wang
Summary: IKK proteins play important roles in the innate immune system of animals. In this study, the CfIKK1 gene was cloned from the Zhikong scallop and its function and signaling were investigated. CfIKK1 was found to respond to pathogen associated molecular patterns (PAMPs) challenge and interact with MyD88 protein, forming dimers or oligomers. RNAi experiments confirmed the regulatory relationship between IKK1 and IRF genes in scallops.
FRONTIERS IN IMMUNOLOGY
(2022)
Editorial Material
Biochemical Research Methods
Nathan P. Manes, Aleksandra Nita-Lazar
Article
Microbiology
Nathan P. Manes, Natalia Shulzhenko, Arthur G. Nuccio, Sara Azeem, Andrey Morgun, Aleksandra Nita-Lazara
Article
Biochemical Research Methods
Mohd M. Khan, Marijke Koppenol-Raab, Minna Kuriakose, Nathan P. Manes, David R. Goodlett, Aleksandra Nita-Lazar
JOURNAL OF PROTEOMICS
(2018)
Article
Biochemical Research Methods
Nathan P. Manes, Aleksandra Nita-Lazar
JOURNAL OF PROTEOMICS
(2018)
Article
Chemistry, Medicinal
Mohd M. Khan, Orna Ernst, Nathan P. Manes, Benjamin L. Oyler, Iain D. C. Fraser, David R. Goodlett, Aleksandra Nita-Lazar
ACS INFECTIOUS DISEASES
(2019)
Article
Biochemical Research Methods
Casey M. Daniels, Pauline R. Kaplan, Isaac Bishof, Clinton Bradfield, Trisha Tucholski, Arthur G. Nuccio, Nathan P. Manes, Samuel Katz, Iain D. C. Fraser, Aleksandra Nita-Lazar
JOURNAL OF PROTEOME RESEARCH
(2020)
Article
Immunology
Reema Bansal, Mohd M. Khan, Surendra Dasari, Indu Verma, David R. Goodlett, Nathan P. Manes, Aleksandra Nita-Lazar, Surya P. Sharma, Aman Kumar, Nirbhai Singh, Anuradha Chakraborti, Vishali Gupta, M. R. Dogra, Jagat Ram, Amod Gupta
Summary: The protein profile in vitreous fluid of patients with TBU differs significantly from non-TBU patients and controls, involving different metabolic and signaling pathways. This differential protein profile provides new insights into the molecular mechanisms of TBU and lays the foundation for exploring biomarkers to differentiate TBU.
Article
Biochemistry & Molecular Biology
Joseph Gillen, Thunnicha Ondee, Devikala Gurusamy, Jiraphorn Issara-Amphorn, Nathan P. Manes, Sung Hwan Yoon, Asada Leelahavanichkul, Aleksandra Nita-Lazar
Summary: Inflammatory response is crucial for resolving infections but can also be harmful. Endotoxin tolerance is a cellular mechanism where repeated exposure to LPS can decrease cellular metabolism and affect the secretion of proteins associated with cell survival, protein metabolism, and reactive oxygen species metabolism.
Article
Microbiology
Orna Ernst, Mohd M. Khan, Benjamin L. Oyler, Sung Hwan Yoon, Jing Sun, Fang-Yu Lin, Nathan P. Manes, Alexander D. MacKerell, Iain D. C. Fraser, Robert K. Ernst, David R. Goodlett, Aleksandra Nita-Lazar
Summary: The innate immune system serves as the body's first line of defense against pathogens, with Toll-like receptor 4 (TLR4) and caspase 11 mediating different signaling pathways leading to cell death and inflammation. Variations in the structure of lipopolysaccharide (LPS) from different Gram-negative bacteria may result in specific immune responses, impacting the selection of therapeutic strategies.
Review
Microbiology
Nathan P. Manes, Aleksandra Nita-Lazar
Summary: Pattern recognition receptors (PRRs) serve as the front line of defense against pathogens. Understanding the molecular mechanisms of PRR signaling is crucial for developing therapeutics for diseases such as sepsis and COVID-19.
Article
Immunology
Zhipeng Li, Manoj Gurung, Richard R. Rodrigues, Jyothi Padiadpu, Nolan K. Newman, Nathan P. Manes, Jacob W. Pederson, Renee L. Greer, Stephany Vasquez-Perez, Hyekyoung You, Kaito A. Hioki, Zoe Moulton, Anna Fel, Dominic De Nardo, Amiran K. Dzutsev, Aleksandra Nita-Lazar, Giorgio Trinchieri, Natalia Shulzhenko, Andrey Morgun
Summary: Microbiota contribute to the induction of type 2 diabetes by causing OXPHOS damage in white adipose tissue. Mmp12(+) macrophages link microbiota-dependent inflammation and OXPHOS damage in WAT.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Nathan P. Manes, Jessica M. Calzola, Pauline R. Kaplan, Iain D. C. Fraser, Ronald N. Germain, Martin Meier-Schellersheim, Aleksandra Nita-Lazar
Summary: This study reports the development and application of targeted mass spectrometry assays to measure the absolute abundance of proteins in the TLR4 and chemotaxis pathways of mouse macrophages. The generated datasets can be used for pathway modeling and simulation, as well as other studies related to TLR and chemotaxis pathways.
Article
Medicine, Research & Experimental
Mark Rochman, Yrina Rochman, Julie M. Caldwell, Lydia E. Mack, John A. Besse, Nathan P. Manes, Sung Hwan Yoon, Tetsuo Shoda, Aleksandra Nita-Lazar, Marc E. Rothenberg
Summary: Eosinophilic esophagitis (EoE) is a chronic gastrointestinal disorder characterized by inflammation and hyperproliferation of esophageal mucosa. A proteomic screen identified the involvement of the minichromosome maintenance (MCM) complex in EoE and suggested MCM inhibitors as potential therapeutic agents for the disease.
Meeting Abstract
Biochemistry & Molecular Biology
Aleksandra Nita-Lazar, Nathan P. Manes, Jessica M. Mann, Pauline Kaplan, Martin Meier-Schellersheim, Iain D. C. Fraser, Ronald N. Germain
Meeting Abstract
Immunology
Aleksandra Nita-Lazar, Virginie Helle Sjoelund, Marijke Koppenol-Raab, Nathan P. Manes, Iain D. Fraser, Zachary Benet, Bhaskar Dutta, Mohd M. Khan
JOURNAL OF IMMUNOLOGY
(2017)