Article
Cell Biology
Dmitriy Karpenko, Nikolay Kapranov, Aleksei Bigildeev
Summary: This study provides direct experimental evidence that mesenchymal stem cells (MSCs) in murine bone marrow are immune privileged and retain functionality after prolonged immune exposure. These findings are important for stem cell research and clinical applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemical Research Methods
Yifei Gao, Haojie Zhang, Jianchun Zhu, Jingxin Li, Yan Tang, Chuanyong Liu
Summary: Enteric neural precursor cells (ENPCs) play an important role in understanding enteric nervous system (ENS)-related diseases in adults. Previous methods faced challenges in isolating and culturing ENPCs due to difficulties in survival, contamination, and low yield. The researchers developed a rapid and effective method to isolate and cultivate ENPCs from adult mice by isolating the longitudinal muscle myenteric plexus (LMMP) from the colon, using papain and collagenase II to increase yield, and promoting growth and proliferation with polylysine precoated culture plates and optimal cell seeding density.
JOURNAL OF NEUROSCIENCE METHODS
(2023)
Article
Developmental Biology
Corey G. K. Flynn, Paul R. Ginkel, Katharine A. Hubert, Qingyuan Guo, Steven M. Hrycaj, Aubrey E. McDermott, Angelo Madruga, Anna P. Miller, Deneen M. Wellik
Summary: Interstitial stromal cells are crucial for muscle development, regeneration, and repair. Hoxa11 and Hoxd11 are expressed in these cells and essential for embryonic patterning of muscles. Hoxa11eGFP expression continues in a subset of muscle interstitial cells through adult stages.
Article
Biology
Muya Shu, Xiaoyu Xue, Hu Nie, Xianming Wu, Minghan Sun, Lianyong Qiao, Xing Li, Bai Xu, Zhifeng Xiao, Yannan Zhao, Yongheng Fan, Bing Chen, Jixiang Zhang, Ya Shi, Yaming Yang, Falong Lu, Jianwu Dai
Summary: This study identified that Nestin(+) cells outside the central canal could be activated to become active NSCs after spinal cord injury, indicating their potential as endogenous NSCs for regenerative therapy in the future.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Akram A. Hamed, Daniel J. Kunz, Ibrahim El-Hamamy, Quang M. Trinh, Omar D. Subedar, Laura M. Richards, Warren Foltz, Garrett Bullivant, Matthaeus Ware, Maria C. Vladoiu, Jiao Zhang, Antony M. Raj, Trevor J. Pugh, Michael D. Taylor, Sarah A. Teichmann, Lincoln D. Stein, Benjamin D. Simons, Peter B. Dirks
Summary: By comparing the developmental stages of mouse cerebrum with glial tumors in human cerebrum, the study suggests that adult gliomas may acquire embryonic-like states during development, providing insight into the origins of human glioma.
NATURE COMMUNICATIONS
(2022)
Article
Polymer Science
Roni Mina Hendler, Orly Eva Weiss, Tzachy Morad, Guy Sion, Michael Kirby, Zvy Dubinsky, Ayan Barbora, Refael Minnes, Danny Baranes
Summary: Our study demonstrates that culturing neural precursor cells (NPCs) on coralline-derived calcium carbonate coated with poly-D-lysine (PDL-CS) promotes their differentiation into reactive astrocytes, potentially accelerating the repair of neural tissue.
Article
Cell & Tissue Engineering
Nicole Baker, Steven Wade, Matthew Triolo, John Girgis, Damian Chwastek, Sarah Larrigan, Peter Feige, Ryo Fujita, Colin Crist, Michael A. Rudnicki, Yan Burelle, Mireille Khacho
Summary: Physiological changes in mitochondrial shape play a crucial role in regulating the quiescent state and activation potential of adult stem cells. Mitochondrial fragmentation promotes the exit from deep quiescence, while loss of the mitochondrial fusion protein OPA1 leads to premature activation and depletion of stem cells.
Article
Pharmacology & Pharmacy
Daiane Cattani, Nona Struyf, Vivien Steffensen, Jonas Bergquist, Ariane Zamoner, Eva Brittebo, Malin Andersson
Summary: Using MALDI IMS, this study examined persistent changes in peptide expression in the substantia nigra (SN) of adult male Wistar rats following perinatal exposure to glyphosate-based herbicide (GBH), revealing significant alterations in peptidergic signaling in the SN. The findings suggest that perinatal GBH exposure may perturb critical neurodevelopmental processes, leading to long-term changes in the SN and hippocampus.
Article
Biology
Deirdre D. Scripture-Adams, Kevin N. Chesmore, Florian Barthelemy, Richard T. Wang, Shirley Nieves-Rodriguez, Derek W. Wang, Ekaterina Mokhonova, Emilie D. Douine, Jijun Wan, Isaiah Little, Laura N. Rabichow, Stanley F. Nelson, M. Carrie Miceli
Summary: This study develops a method to isolate and sequence individual nuclei from frozen muscle, allowing for the exploration of cellular and transcriptomic mechanisms in Duchenne muscular dystrophy. The findings reveal the effects of exon skipping therapy on muscle nuclei and demonstrate partial rescue of muscle fibers, expansion of myeloid population, recovery of M2 macrophages, and repression of inflammatory fibroblasts.
COMMUNICATIONS BIOLOGY
(2022)
Article
Genetics & Heredity
Pengcheng Lyu, Yumin Qi, Zhijian J. Tu, Honglin Jiang
Summary: This study analyzed bovine satellite cells using single-cell RNA sequencing and identified subpopulations of satellite cells with different gene expression profiles, showing variations in proliferation rate and differentiation stage. Some satellite cells were enriched with myocyte markers, while others were enriched with markers of fibro-adipogenic cells, suggesting a heterogeneous composition of bovine satellite cells.
FRONTIERS IN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Ikumi Ohsawa, Fuminori Kawano
Summary: This study showed that exercise training activates histone turnover in skeletal muscle fibers, leading to nucleosome loosening and enhanced gene responses to exercise.
Article
Health Care Sciences & Services
Karyn G. Robinson, Adam G. Marsh, Stephanie K. Lee, Jonathan Hicks, Brigette Romero, Mona Batish, Erin L. Crowgey, M. Wade Shrader, Robert E. Akins
Summary: This study examined the DNA methylation differences in myoblasts and myotubes derived from satellite cells in patients with spastic cerebral palsy (CP). The results showed significant DNA methylation differences in these cells, suggesting potential new targets for studying the mechanisms contributing to muscle dysregulation in spastic CP.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Tetsuro Tamaki, Toshiharu Natsume, Akira Katoh, Nobuyuki Nakajima, Kosuke Saito, Tsuyoshi Fukuzawa, Masayoshi Otake, Satoko Enya, Akihisa Kangawa, Takeshi Imai, Miyu Tamaki, Yoshiyasu Uchiyama
Summary: This study investigates the differentiation potential of pig skeletal muscle-derived stem cells (Sk-MSCs) for nerve muscle regenerative therapy. The results show that these cells have a high capacity to differentiate into skeletal muscle, peripheral nerve, and vascular cell lineages, making them a valuable intermediate model between mice and humans. This research contributes to preclinical studies of regenerative stem cell transplantation therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Xhem Qabrati, Inseon Kim, Adhideb Ghosh, Nicola Bundschuh, Falko Noe, Andrew S. Palmer, Ori Bar-Nur
Summary: This study presents an improved approach to convert fibroblasts into induced myogenic progenitor cells (iMPCs) without the use of viral vectors. By overexpressing synthetic MyoD-mRNA and inhibiting the JNK and JAK/STAT pathways, fibroblasts were reprogrammed into transgene-free iMPCs that exhibited myogenic stem cell characteristics and could differentiate into functional myotubes. Transgene-free iMPCs also showed successful engraftment and restoration of dystrophin expression in a Duchenne muscular dystrophy mouse model, suggesting their potential for muscle regeneration in vivo.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Yalan Yang, Xinhao Fan, Junyu Yan, Muya Chen, Min Zhu, Yijie Tang, Siyuan Liu, Zhonglin Tang
Summary: The study generated the first single-base resolution DNA methylome and transcriptome maps of porcine skeletal muscle across 27 developmental stages. The results showed a decrease in overall methylation level from embryo to adult, and identified over 40,000 developmentally differentially methylated CpGs.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Virology
Jessica Pires Farias, Patricia de Souza da Silva, Mayanna Moreira Costa Fogaca, Itana Vivian Rocha Santana, Wilson Barros Luiz, Alexander Birbrair, Jaime Henrique Amorim
Summary: Vaccination can significantly reduce the number of infected individuals, although there is a downward trend in specific antibody levels after vaccination, the use of booster shots with Pfizer can increase specific SARS-CoV-2 IgG serum levels. Vaccination is more effective than natural infection with SARS-CoV-2.
JOURNAL OF VIROLOGY
(2022)
Editorial Material
Infectious Diseases
Jaime Henrique Amorim, Alexander Birbrair
LANCET INFECTIOUS DISEASES
(2022)
Article
Peripheral Vascular Disease
Beatriz G. S. Rocha, Caroline C. Picoli, Bryan O. P. Goncalves, Walison N. Silva, Alinne C. Costa, Michele M. Moraes, Pedro A. C. Costa, Gabryella S. P. Santos, Milla R. Almeida, Luciana M. Silva, Youvika Singh, Marcelo Falchetti, Gabriela D. A. Guardia, Pedro P. G. Guimaraes, Remo C. Russo, Rodrigo R. Resende, Mauro C. X. Pinto, Jaime H. Amorim, Vasco A. C. Azevedo, Alexandre Kanashiro, Helder I. Nakaya, Edroaldo L. Rocha, Pedro A. F. Galante, Akiva Mintz, Paul S. Frenette, Alexander Birbrair
Summary: This study reveals the presence of perivascular GFAP+/Plp1+ cells derived from tissue-resident Schwann cells in the tumor microenvironment. Genetic ablation of Schwann cells slows down tumor growth and angiogenesis, while boosting immune surveillance by increasing tumor-infiltrating anti-tumor lymphocytes and reducing immune-suppressor cells. In humans, increased expression of Schwann cell-related genes within melanoma is associated with improved survival.
Review
Biochemistry & Molecular Biology
Patricia de Carvalho Ribeiro, Lucas Felipe Oliveira, Daniel Mendes Filho, Ricardo Cambraia Parreira, Mariana Sousa Vieira, Bruno Lemes Marques, Elis Marra da Madeira Freitas, Walison N. N. Silva, Helton da Costa Santiago, Alexander Birbrair, Henning Ulrich, Valdo Jose Dias da Silva, Rodrigo R. R. Resende
Summary: Systemic arterial hypertension is a major risk factor for cardiovascular and renal diseases, and early recognition is important for prevention. The study of biomarkers is an important approach for diagnosing and predicting hypertension and related conditions. This review discusses the use of biomarkers in hypertension and associated diseases such as ischemic stroke, intracerebral hemorrhage, transient ischemic attack, acute myocardial infarction, angina pectoris, and chronic kidney disease. Establishing a pool of potential biomarkers can improve diagnosis, prognosis, risk assessment, therapy management, and pharmacological responses to enhance patients' quality of life and prevent unfavorable outcomes.
CURRENT MEDICINAL CHEMISTRY
(2023)
Review
Cell Biology
Lucas Felipe de Oliveira, Daniel Mendes Filho, Bruno Lemes Marques, Giovana Figueiredo Maciel, Ricardo Cambraia Parreira, Jose Rodrigues do Carmo Neto, Priscilla Elias Ferreira Da Silva, Rhanoica Oliveira Guerra, Marcos Vinicius da Silva, Helton da Costa Santiago, Alexander Birbrair, Alexandre H. Kihara, Valdo Jose Dias da Silva, Talita Glaser, Rodrigo R. Resende, Henning Ulrich
Summary: Infectious diseases have significant impacts on human health globally, and there is an urgent need for a better understanding. In vitro and in vivo infection models have made significant contributions to our current knowledge of these diseases. Organoids simulate tissue architecture and function, recapitulating many characteristics of human diseases and elucidating host-pathogen interactions in various systems. In this article, we discuss the applicability of organoid technology in modeling pathogenesis, host response, and features, which can be explored for the development of preventive and therapeutic treatments.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Bruno L. Marques, Giovana Figueiredo Maciel, Marcello R. Brito Junior, Lucas D. Dias, Sergio Scalzo, Anderson K. Santos, Alexandre Hiroaki Kihara, Helton da Costa Santiago, Ricardo C. Parreira, Alexander Birbrair, Rodrigo R. Resende
Summary: As the world population's life expectancy increases, more efficient therapies are needed for neurodegenerative disorders common in old age. Neural stem cells (NSCs) play a crucial role in the development and functioning of the nervous system and have broad therapeutic applications for neurodegenerative diseases. Understanding the mechanisms that govern NSC self-renewal, differentiation, and cell signaling is essential for these therapies.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Anderson K. Santos, Sergio Scalzo, Raysa T. V. de Souza, Pedro H. G. Santana, Bruno L. Marques, Lucas F. Oliveira, M. Filho Daniel, Alexandre Hiroaki Kihara, Helton da Costa Santiago, Ricardo C. Parreira, Alexander Birbrair, Henning Ulrich, Rodrigo R. Resende
Summary: Organoid development and organ-on-a-chip are technologies that utilize stem cells to create 3D multicellular structures resembling organs and tissues in vivo. They can be strategically employed for disease modeling, drug screening, and host-pathogen studies.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Neurosciences
Mariana Ribeiro Costa, Amanda Yasmin Ilario dos Santos, Tais Browne de Miranda, Rogerio Aires, Alex de Camargo Coque, Elizabeth Cristina Perez Hurtado, Maria Martha Bernardi, Vanessa Gallego Arias Pecorari, Denise Carleto Andia, Alexander Birbrair, Gilles J. Guillemin, Alexandra Latini, Rodrigo A. da Silva
Summary: Sonic Hedgehog (Shh) signaling plays a critical role in CNS development and its dysregulation leads to neurological disorders. However, the regulation of Shh signaling in the adult brain is not well understood. This study investigated the effects of DNA methylation on the transcriptional control of Shh signaling pathway members as well as its modulation by inflammation. The findings demonstrate the importance of methylation in the promoter regions of Shh pathway members in determining transcriptional patterns in the adult brain, and reveal the impact of neuroinflammation on the transcriptional control of these genes.
Article
Biotechnology & Applied Microbiology
Gabriela Munis Campos, Monique Ferrary Americo, Andria dos Santos Freitas, Fernanda Alvarenga Lima Barroso, Joyce da Cruz Ferraz Dutra, Ludmila Silva Quaresma, Barbara Fernandes Cordeiro, Juliana Guimaraes Laguna, Luis Claudio Lima de Jesus, Aparecida Maria Fontes, Alexander Birbrair, Tulio Marcos Santos, Vasco Azevedo
Summary: Using probiotics as vehicles for delivering therapeutic agents with anti-inflammatory properties through recombinant protein expression is a promising approach for preventing and treating various diseases, such as cancer and intestinal immune disorders.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Biochemistry & Molecular Biology
Salvador Sanchez-Vinces, Pedro Henrique Dias Garcia, Alex Ap. Rosini Silva, Anna Maria Alves de Piloto Fernandes, Joyce Aparecida Barreto, Gustavo Henrique Bueno Duarte, Marcia Aparecida Antonio, Alexander Birbrair, Andreia M. Porcari, Patricia de Oliveira Carvalho
Summary: The comprehensive lipidome determined by mass spectrometry is correlated to the serum whole-lipidic profile of adults with different lipidemic conditions. Specifically, serum TAG levels have a positive correlation with certain specific TAGs and ceramides, and a negative correlation with sphingomyelins. HDL-C levels are positively correlated with certain groups of glycerophosphocholine, while LDL-C has a positive correlation with sphingomyelins.
Article
Multidisciplinary Sciences
Isabella Bittencourt do Valle, Sicilia Rezende Oliveira, Janine Mayra da Silva, Gabriela Tonini Peterle, Anna Clara Gregorio Co, Sebastiao Silverio Sousa-Neto, Elismauro Francisco Mendonca, Jose Alcides Almeida de Arruda, Natalia Aparecida Gomes, Gabriel da Silva, Andreia Machado Leopoldino, Soraia Macari, Alexander Birbrair, Sandra Ventorin von Zeidler, Ivana Marcia Alves Diniz, Tarcilia Aparecida Silva
Summary: This study investigates the role of pericytes in oral squamous cell carcinoma (OSCC) and finds that they interact with neoplastic cells, influencing cancer progression and outcomes. The study characterizes pericytes in clinical samples and a mouse model of oral carcinogenesis, and shows that an increase in pericyte numbers is associated with the development of oral cancer.
SCIENTIFIC REPORTS
(2023)
Article
Instruments & Instrumentation
Ana Luiza de Castro Santos, Natalia Jordana Alves da Silva, Celso Tarso Rodrigues Viana, Leticia Cristine Cardoso dos Santos, Gabriel Henrique Costa da Silva, Sergio Ricardo Aluotto Scalzo Junior, Pedro Augusto Carvalho Costa, Walison Nunes da Silva, Itamar Couto Guedes de Jesus, Alexander Birbrair, Mariana T. Q. de Magalhaes, Frederic Frezard, Silvia Guatimosim, Rebecca M. Haley, Michael J. Mitchell, Silvia Passos Andrade, Paula Peixoto Campos, Pedro Pires Goulart Guimaraes
Summary: This study investigated the effects of the Wnt inhibitor complex CD: LGK974 on reducing the inflammatory, fibrotic, and angiogenic processes caused by intraperitoneal implants. The results showed that CD: LGK974 significantly reduced immune cell infiltration, inflammatory cytokine release, collagen deposition, and expression of pro-fibrotic markers. It also decreased VEGF levels and inhibited blood vessel formation. These findings suggest that the use of the Wnt inhibitor complex may be a potential strategy to reduce peritoneal fibrosis and implant rejection.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Oncology
Vincent Pozorski, Yeonhee Park, Yusuf Mohamoud, Dahlia Tesfamichael, Hamid Emamekhoo, Alexander Birbrair, Mark R. Albertini, Vincent T. Ma
Summary: Neutrophil-to-lymphocyte ratios (NLR) and eosinophil counts are associated with improved survival in melanoma patients treated with immune checkpoint inhibitors. This study investigated neutrophil-to-eosinophil ratios (NER) as a predictive indicator in this population and found that lower baseline NER is associated with better treatment response and survival.
PIGMENT CELL & MELANOMA RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Laisa Macedo Tavares, Luis Claudio Lima de Jesus, Viviane Lima Batista, Fernanda Alvarenga Lima Barroso, Andria dos Santos Freitas, Gabriela Munis Campos, Monique Ferrary Americo, Tales Fernando da Silva, Nina Dias Coelho-Rocha, Giovanna Angeli Belo, Mariana Martins Drumond, Pamela Mancha-Agresti, Katia Duarte Vital, Simone Odilia Antunes Fernandes, Valbert Nascimento Cardoso, Alexander Birbrair, Enio Ferreira, Flaviano Santos Martins, Juliana Guimaraes Laguna, Vasco Azevedo
Summary: Intestinal mucositis is frequently observed in cancer patients undergoing chemotherapy and radiotherapy. Probiotics, prebiotics, and synbiotics have been investigated as alternative treatments for intestinal mucositis due to their anti-inflammatory properties. This study evaluated the anti-inflammatory effects of a synbiotic formulation containing L. delbrueckii CIDCA 133 and FOS in mice with 5-FU-induced intestinal mucosa inflammation. The results showed that the synbiotic formulation could modulate inflammatory parameters, protect the intestinal mucosa from damage, and improve the epithelial barrier function.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Caroline C. C. Picoli, Patricia Rocha Martins, Xiao Lin Casey Wong, Thamires Righi, Pedro P. G. Guimaraes, Mauro C. X. Pinto, Jaime H. H. Amorim, Vasco A. C. Azevedo, Silma Regina Pereira, Alexandre Kanashiro, Fabio Cardoso Cruz, Rodrigo R. R. Resende, Akiva Mintz, Paul S. S. Frenette, Alexander Birbrair
Summary: Understanding the bone marrow niche is crucial for maintaining hematopoietic stem cells. This study presents a new method, called "whole bone subcutaneous transplantation", to precisely study the bone marrow niche in transgenic animals. By labeling cells, researchers were able to identify specific cellular components of the niche and found that certain cells play an important role in maintaining hematopoietic stem cells.
STEM CELL REVIEWS AND REPORTS
(2023)