Article
Neurosciences
Diana Spicarova, Vladimir Nerandzic, David Muzik, Monica Pontearso, Anirban Bhattacharyya, Istvan Nagy, Jiri Palecek
Summary: Through research on neurons in rat acute spinal cord slices, it was found that the precursor of anandamide, 20:4-NAPE, can modulate spinal cord nociceptive signaling through TRPV1 and CB1 receptors, especially under inflammatory conditions. This finding is of great significance for the development of pain.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Cell Biology
Meera Manchanda, Emma Leishman, Kishan Sangani, Ali Alamri, Heather B. Bradshaw
Summary: This study investigates how activation of TRPV1 drives regulation of a wider range of lipid signaling molecules, which is time and dose-dependent. The analysis of lipids revealed that capsaicin increases the release of 2-acyl glycerols while decreasing levels of N-acyl ethanolamines. Changes in temperature also result in similar regulation of lipid signaling molecules.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Diane M. Bogdan, Keith Studholme, Adriana DiBua, Chris Gordon, Martha P. Kanjiya, Mei Yu, Michelino Puopolo, Martin Kaczocha
Summary: The endocannabinoid anandamide can produce both anti-pain effects and exacerbate pain. FABP5 plays a key role in regulating the levels of endocannabinoids in nociceptors, affecting pain perception. Decreasing the expression of FABP5 can increase the levels of endocannabinoids, leading to anti-pain effects.
SCIENTIFIC REPORTS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Gaia Pellegrini, Daniela Carmagnola, Marilisa Toma, Giulio Rasperini, Marica Orioli, Claudia Dellavia
Summary: The aim of this study was to assess the involvement of the endocannabinoid system in patients with recurrent and non-recurrent periodontal disease, and to determine if levels of anandamide (AEA) change after periodontal therapy. The results showed that the endocannabinoid system is involved differently in patients with recurrent and non-recurrent periodontal disease.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Francesco Manfrevola, Nicoletta Potenza, Teresa Chioccarelli, Armando Di Palo, Chiara Siniscalchi, Veronica Porreca, Arcangelo Scialla, Vincenza Grazia Mele, Giuseppe Petito, Aniello Russo, Antonia Lanni, Rosalba Senese, Giulia Ricci, Riccardo Pierantoni, Rosanna Chianese, Gilda Cobellis
Summary: The study highlights the role of cannabinoid receptor CB1 in regulating sperm circular RNA cargo, which influences sperm morphology and cellular maturation. Specifically, circLIMA1 is identified as a potential regulator of cytoskeletal actin and plays a role in sperm nuclear actin remodeling during epididymal maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Neurosciences
Lia P. Iglesias, Heliana B. Fernandes, Aline S. de Miranda, Malena M. Perez, Lucia H. Faccioli, Carlos A. Sorgi, Leandro J. Bertoglio, Daniele C. Aguiar, Carsten T. Wotjak, Fabricio A. Moreira
Summary: This study found that hippocampal TRPV1 channels play a role in the modulation of fear memories. Endocannabinoid signaling may mediate the effects of TRPV1 blockers. The intensity of the aversive stimulus affects the regulation of fear memories by TRPV1 channels. TRPV1 blockers also promote the transcription of specific genes, leading to a decrease in fear memory reinstatement.
Review
Biochemistry & Molecular Biology
Giuditta Gambino, Daniele Gallo, Ana Covelo, Giuseppe Ferraro, Pierangelo Sardo, Giuseppe Giglia
Summary: This study investigated the involvement of TRPV1 in nitric oxide-mediated influence on hippocampal processes. The findings suggest that TRPV1 channels can be activated to modulate excitatory neurotransmission in the hippocampus, and this effect is linked to nitric oxide production.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Kohei Umezu, Shouhei Kurata, Kenshiro Hara, Kentaro Tanemura
Summary: The study revealed the roles of caffeine and thimerosal in sperm detachment from agglutination in bulls, demonstrating caffeine as a trigger, hyperactivated motility facilitating detachment, and the phenomenon of sperm reagglutination.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Sepideh Khaksar, Mona Salimi, Hadi Zeinoddini, Nima Naderi
Summary: This research investigated the involvement of CB1 and TRPV1 receptors in the protective effects of anandamide in epilepsy. The results showed that anandamide decreased seizure scores, while the TRPV1 and CB1 antagonists had no effect on seizure scores. Co-administration of the antagonists attenuated the protective effect of anandamide. Furthermore, anandamide led to a decrease in the expressions of CREB and p-CREB in hippocampal neurons.
NEUROCHEMICAL RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Stewart Ramsay, Nick J. Spencer, Vladimir Zagorodnyuk
Summary: The aim of this study is to determine the role of endocannabinoids, anandamide (AEA) and 2-arachidonolylglycerol (2-AG), in regulating mechanosensitivity of probable nociceptive neurons innervating the bladder - capsaicin-sensitive mucosal afferents. The results showed that AEA potentiated the mechanical response of mucosal afferents, while 2-AG inhibited their response. These findings have important implications for understanding the role of endocannabinoids in regulating bladder sensation and function.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Marina Ramal-Sanchez, Nicola Bernabo, Luca Valbonetti, Costanza Cimini, Angela Taraschi, Giulia Capacchietti, Juliana Machado-Simoes, Barbara Barboni
Summary: TRPV1, a polymodal sensor, is extensively studied and plays important roles in various key events in post-ejaculatory life of sperm. This review gathers all the known modulators of TRPV1 function and their relationship with reproductive processes, providing insights into the indirect participation of TRPV1 receptor during sperm migration events such as chemotaxis, thermotaxis, and rheotaxis. Additionally, TRPV1 is highlighted as a potential pharmacological target in male reproductive system-related pathologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
Md Mazharul Islam, Takashi Umehara, Natsumi Tsujita, Masanori Koyago, Masayuki Shimada
Summary: This study demonstrates that exogenous cholesterol treatment can enhance the quality of frozen-thawed bull sperm by restoring the cholesterol balance in sperm plasma and organelle membranes. The addition of exogenous cholesterol protects the plasma membrane and maintains mitochondrial function, thereby improving sperm motility and fertility. Therefore, the application of cholesterol after thawing is a promising option for improving the fertility of frozen-thawed sperm.
MOLECULAR HUMAN REPRODUCTION
(2023)
Article
Cell Biology
Wanglong Xiao, Ying Chen
Summary: This paper comprehensively reviews the expression and mechanism of TRPV1 in the male reproductive system from molecular and cellular perspectives, with a focus on sperm function, providing strategies for tackling male infertility.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Maria Morena, Andrei S. Nastase, Alessia Santori, Benjamin F. Cravatt, Rebecca M. Shansky, Matthew N. Hill
Summary: The endocannabinoid system has different effects on fear extinction in males and females, with increased AEA signaling impairing extinction in females, while inhibition of 2-AG hydrolysis promoting active fear responses. Sex- and brain region-specific changes were also observed in AEA and 2-AG levels after extinction training. These findings may have implications for sex-specific therapeutics in trauma-related disorders.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Veterinary Sciences
Amer Salman, J. Nestor Caamano, Estela Fernandez-Alegre, Carlos O. Hidalgo, Touba Nadri, Carolina Tamargo, Carmen Fueyo, Angel Fernandez, Maria J. Merino, Felipe Martinez-Pastor
Summary: The study aimed to test the antioxidant effects of curcumin and crocin as supplements to freezing extenders, with crocin significantly decreasing sperm viability and increasing apoptotic features, while curcumin increased ROS production and motility.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Reproductive Biology
Bidur Paudel, Maria Gracia Geryasi, James Porambo, Diego A. Caraballo, Darya A. Tourzani, Jesse Mager, Mark D. Platt, Ana Maria Salicioni, Pablo E. Visconti
BIOLOGY OF REPRODUCTION
(2019)
Article
Cell Biology
Ana Romarowski, Angel G. Velasco Felix, Paulina Torres Rodriguez, Maria G. Gervasi, Xinran Xu, Guillermina M. Luque, Gaston Contreras-Jimenez, Claudia Sanchez-Cardenas, Hector V. Ramirez-Gomez, Diego Krapf, Pablo E. Visconti, Dario Krapf, Adan Guerrero, Alberto Darszon, Mariano G. Buffone
JOURNAL OF CELL SCIENCE
(2018)
Article
Biochemistry & Molecular Biology
Eduardo Martinez-Leon, Claudia Osycka-Salut, Janetti Signorelli, Milene Kong, Patricio Morales, Silvina Perez-Martinez, Emilce Silvina Diaz
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2019)
Article
Cell Biology
C. A. I. Alonso, R. Lottero-Leconte, G. M. Luque, Z. J. Vernaz, N. Di Siervi, M. G. Gervasi, M. G. Buffone, C. Davio, S. Perez-Martinez
JOURNAL OF CELL SCIENCE
(2019)
Article
Reproductive Biology
C. E. Osycka-Salut, E. Martinez-Leon, M. G. Gervasi, L. Castellano, C. Davio, N. Chiarante, A. M. Franchi, M. L. Ribeiro, E. S. Diaz, S. Perez-Martinez
Article
Multidisciplinary Sciences
Nicolas Chiarante, Carlos A. I. Alonso, Jessica Plaza, Raquel Lottero-Leconte, Camila Arroyo-Salvo, Agustin Yaneff, Claudia E. Osycka-Salut, Carlos Davio, Marcelo Miragaya, Silvina Perez-Martinez
SCIENTIFIC REPORTS
(2020)
Review
Agriculture, Dairy & Animal Science
Maria G. Gervasi, Ana J. Soler, Lauro Gonzalez-Fernandez, Marco G. Alves, Pedro F. Oliveira, David Martin-Hidalgo
Article
Agriculture, Dairy & Animal Science
Brenda Florencia Gimeno, Maria Victoria Bariani, Lucia Laiz-Quiroga, Eduardo Martinez-Leon, Micaela Von-Meyeren, Osvaldo Rey, Adrian Angel Mutto, Claudia Elena Osycka-Salut
Summary: This study improved the yield of fertilization-capable sperm from equine frozen sperm by optimizing manipulation conditions and establishing a primary culture model of oviductal cells. The in vitro model demonstrated the potential fertilizing capacity of sperm released from oviductal cells, suggesting advancements in handling and selection of cryopreserved sperm for improved efficiencies in assisted reproductive techniques.
Article
Biology
Feng Wang, Maria Gracia Gervasi, Ana Boskovic, Fengyun Sun, Vera D. Rinaldi, Jun Yu, Mary C. Wallingford, Darya A. Tourzani, Jesse Mager, Lihua Julie Zhu, Oliver J. Rando, Pablo E. Visconti, Lara Strittmatter, Ingolf Bach
Summary: The X-linked gene Rlim plays a crucial role in maintaining imprinted X chromosome inactivation in female mouse embryos, while also being highly expressed in post-meiotic round spermatids and Sertoli cells in the testes. Systemic deletion of Rlim results in lower numbers of mature sperm with reduced motility, highlighting the importance of Rlim in male reproduction, specifically in round spermatids during spermiogenesis.
Article
Biochemistry & Molecular Biology
Guillermina M. Luque, Xinran Xu, Ana Romarowski, Maria G. Gervasi, Gerardo Orta, Jose L. De la Vega-Beltran, Cintia Stival, Nicolas Gilio, Tomas Dalotto-Moreno, Dario Krapf, Pablo E. Visconti, Diego Krapf, Alberto Darszon, Mariano G. Buffone
Summary: The study found that Cdc42 and CatSper play crucial roles in sperm, with Cdc42 inhibition affecting CatSper activity and severely compromising the sperm's fertilizing potential. Cdc42 regulates the function of CatSper by modulating the production of cAMP, providing a new regulatory mechanism for stimulating CatSper through the cAMP-dependent pathway.
Article
Reproductive Biology
L. Castellano, C. A. Arroyo-Salvo, N. Chiarante, C. A. Alonso, R. M. Lottero-Leconte, Z. J. Vernaz, M. Navarro, A. Mutto, C. Osycka-Salut, M. L. Ribeiro, S. Perez-Martinez
Summary: This study aimed to investigate the correlation between the localization of alpha 5 beta 1 integrin and the fertilizing ability of bovine cryopreserved semen samples. The presence of alpha 5 beta 1 integrin in the postacrosomal region showed a positive correlation with in vitro fertilization rate, suggesting it as a potential marker for predicting reproductive performance accurately.
Review
Agriculture, Dairy & Animal Science
Luis Aguila, Claudia Osycka-Salut, Favian Treulen, Ricardo Felmer
Summary: This review discusses the pluripotency features, pluripotency genes, and chemical strategies for controlling pluripotency in vitro cultured bovine embryos, as well as the applicability of manipulating pluripotency in the transition from morula to blastocyst in cattle species.
Article
Biochemistry & Molecular Biology
Darya A. A. Tourzani, Qiangzong Yin, Erica A. A. Jackson, Oliver J. J. Rando, Pablo E. E. Visconti, Maria G. G. Gervasi
Summary: The sperm energy restriction and recovery (SER) treatment developed in the laboratory improves fertilization and blastocyst development in mice. The effects of SER on early embryogenesis were investigated, showing advanced progression through the pronuclear stages and changes in histone modifications and gene expression. These findings suggest that sperm incubation conditions can impact early embryo development and improve assisted reproductive technology outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Ferran Barrachina, Kiera Ottino, Maia Lina Elizagaray, Maria Gracia Gervasi, Leona J. Tu, Styliani Markoulaki, Raul G. Spallanzani, Diane Capen, Dennis Brown, Maria Agustina Battistone
Summary: Regulatory T cells play a crucial role in maintaining the tolerogenic state of the testis and epididymis. Depletion of Tregs leads to autoimmune orchitis and epididymitis, affecting sperm production and leading to fertility defects. Understanding these immunoregulatory mechanisms could aid in the development of new treatments for male infertility and immunocontraception.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Darya A. Tourzani, Bidur Paudel, Patricia Miranda, Pablo E. Visconti, Maria G. Gervasi
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2018)