Article
Multidisciplinary Sciences
Delia Tomoiaga, Jaclyn Bubnell, Liam Herndon, Paul Feinstein
Summary: Griffith proposed the concept of DNA transfer between bacteria in 1928. Researchers have demonstrated that E. coli can accept multiple plasmids simultaneously, resulting in widespread aclonality during colony development. Tracking clonally related bacteria allows for mosaic analysis of gene function. Additionally, it was discovered that clonally related bacterium rods self-organize fractally and can remain linked during colony growth, presenting a potential target for controlling microbiota growth.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Helmut Hirt, Kerryl E. Greenwood-Quaintance, Aaron M. T. Barnes, Melissa J. Karau, Lisa M. Till, Elise Palzer, Weihua Guan, Michael S. VanNieuwenhze, Purna C. Kashyap, Robin Patel, Gary M. Dunny
Summary: Microbial communities protect their hosts by excluding pathogenic invaders, and plasmid transfer and effects on host colonization of Enterococcus faecalis in the intestinal tract of mice play a key role in this process.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Enrico Orsi, Nico J. Claassens, Pablo Nikel, Steffen N. Lindner
Summary: Synthetic biology has revolutionized the ability to redesign microbial metabolic networks by combining metabolic pathway-modularization with growth-coupled selection schemes. This powerful tool enables deep rewiring of cell factories' biochemistry for rational bioproduction.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Micjel Chavez Morejon, Alexander Franz, Rohan Karande, Falk Harnisch
Summary: We demonstrate a method for synthesizing adipic acid (AA), a key component of polyamides like Nylon-6,6, from lignin-derived feedstock using a combination of electrochemical hydrogenation and biotransformation. The electrochemical hydrogenation process achieves a 68% yield of cyclohexanol from phenol at a 69% coulombic efficiency, and the electrochemical hydrogenation of an aromatic mixture obtained from lignin depolymerization yields up to 83%. By further biotransformation with recombinant Pseudomonas taiwanensis VLB120, AA is produced at a rate of 0.02 g L-1 h(-1) with a yield of up to 61% in 5 hours.
Article
Agriculture, Multidisciplinary
Huijing Wang, Lian Wang, Jianbin Chen, Minglong Hu, Fang Fang, Jingwen Zhou
Summary: By preparing an edible oil-based lipid nanoparticle (LNP) system, the oral bioaccessibility of flavonoids, including quercetin, naringenin, and hesperetin, was enhanced. In simulated digestion assays, LNPs were hydrolyzed under small intestine conditions, successfully protecting the encapsulated flavonoids, and the relative bioaccessibility of flavonoids was increased to more than 71%.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yi-feng Huang, Yi Li, Jie-ying Chen, Jia-hui Lin, Lu Liu, Jin-zhou Ye, Yu-bin Su
Summary: This study explores the combined effection of metal ions and antibiotics on the drug resistance of Escherichia coli. The results showed that exogenous Fe3+ could decrease intracellular gentamicin concentration and reduce reactive oxygen species (ROS) production, leading to the restoration of gentamicin-mediated killing by Fe3+.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Genetics & Heredity
Adrien Knoops, Laura Ledesma-Garcia, Alexandra Waegemans, Morgane Lamontagne, Baptiste Decat, Herve Degand, Pierre Morsomme, Patrice Soumillion, Frank Delvigne, Pascal Hols
Summary: Competence for DNA transformation is a major bacterial adaptation and survival strategy. In this study, we investigated the mechanism of competence shut-off in Streptococcus salivarius. Through mathematical modeling and experimental validation, we identified the reciprocal role of PepF and XIP in ComRS shut-off.
Article
Multidisciplinary Sciences
Nicolas Waisbord, Amin Dehkharghani, Jeffrey S. Guasto
Summary: Through microfluidic experiments, it was found that individual magnetotactic bacteria display three distinct regimes when directed upstream through pores, resembling the electrical conductivity of a diode. This diode-like behavior persists at the pore scale, impacting flow conductivity in higher dimensional geometries. The study has implications for magnetotactic bacteria survival strategies in sediments and potential drug delivery applications in vascular networks.
NATURE COMMUNICATIONS
(2021)
Article
Physics, Fluids & Plasmas
Alidivinas Prusokas, Michelle Hawkins, Conrad A. Nieduszynski, Renata Retkute
Summary: Cell plating, a common laboratory procedure in microbiology, involves spreading liquid suspension of cells on a surface for colony growth. The impact of parameters on colony growth has not been extensively studied, but investigating these parameters can help to better understand and control the outputs of microbiology techniques.
Article
Biology
Josiah C. Kratz, Shiladitya Banerjee
Summary: A quantitative theory is developed to explain the relationship between bacterial growth and division rates and proteome allocation in time-varying nutrient environments. The theory shows that in such environments, cell size and growth are regulated by trade-offs between biomass accumulation and division. The study also finds that bacteria exhibit a transient memory of previous metabolic states, allowing for faster adaptation to previously encountered environments.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xiaoying He, Yan Li, Yehui Tao, Xuelian Qi, Ruiqi Ma, Honghua Jia, Ming Yan, Kequan Chen, Ning Hao
Summary: Sucrose phosphorylase (SPase, EC2.4.1.7) is an important enzyme for producing glycosylated compounds. In this study, an attempt was made to extracellularly express a recombinant SPase from Thermobacillus sp. in E. coli, but the enzyme was unexpectedly produced outside the cells. Truncation experiments showed that both termini of the enzyme are crucial for protein folding and enzymatic activity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Multidisciplinary Sciences
Kazuki Kato, Sae Okazaki, Cian Schmitt-Ulms, Kaiyi Jiang, Wenyuan Zhou, Junichiro Ishikawa, Yukari Isayama, Shungo Adachi, Tomohiro Nishizawa, Kira S. Makarova, Eugene V. Koonin, Omar O. Abudayyeh, Jonathan S. Gootenberg, Hiroshi Nishimasu
Summary: The type III-E CRISPR-Cas7-11 effector can catalyze crRNA-guided RNA cleavage by binding crRNA and Csx29, and the binding of tgRNA induces conformational changes in Csx29. Csx29 cleaves Csx30 through a tgRNA-dependent pathway, resulting in the generation of toxic protein fragments and growth arrest, which is regulated by Csx31. Csx30 modulates the cellular response to infection by binding Csx31 and the associated sigma factor RpoE. Additionally, the Cas7-11-Csx29-Csx30 system has been engineered for programmable RNA sensing in mammalian cells.
Article
Infectious Diseases
Daan W. Notermans, Annelot F. Schoffelen, Fabian Landman, Cornelia C. H. Wielders, Sandra Witteveen, Varisha A. Ganesh, Marga Van Santen-Verheuvel, Sabine C. de Greeff, Ed J. Kuijper, Antoni P. A. Hendrickx
Summary: Urine-associated Escherichia coli ST38 producing OXA-244 harboring putative uropathogenicity factors emerged in the Netherlands.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Multidisciplinary Sciences
Marc Teufel, Carlo A. Klein, Maurice Mager, Patrick Sobetzko
Summary: CRISPR SWAPnDROP is a novel genome editing technology that enables large-scale in-vivo DNA transfer between bacterial species. It offers a modular platform and a multi-color scarless co-selection system, facilitating genome editing in various species.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Waleska Saitz, David A. Montero, Mirka Pardo, Daniela Araya, Marjorie De la Fuente, Marcela A. Hermoso, Mauricio J. Farfan, Daniel Ginard, Ramon Rossello-Mora, Dave A. Rasko, Felipe Del Canto, Roberto M. Vidal
Summary: The study aimed to identify molecular markers for screening AIEC strains. By analyzing outer membrane proteins and genes in AIEC strains, the study found that chuA, eefC, and fitA genes are significantly correlated with AIEC strains and can serve as markers for AIEC strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)