Article
Medicine, General & Internal
Ji Hye Im, Tae Hoon Kim, Kyue-Yim Lee, Ho-Shin Gwak, Weiwei Lin, Jong Bae Park, Jong Heon Kim, Byong Chul Yoo, Seong-Min Park, Ji-Woong Kwon, Sang Hoon Shin, Heon Yoo
Summary: The study demonstrated the potential of extracellular microRNAs in cerebrospinal fluid as biomarkers for the diagnosis and pathogenesis study of leptomeningeal metastasis (LM). The unique miRNA profile in LM compared to brain metastasis (BM) suggests the importance of miRNAs in understanding LM pathogenesis.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Plant Sciences
Rohit Bharati, Madhab Kumar Sen, Ram Kumar, Aayushi Gupta, Jana Ziarovska, Eloy Fernandez-Cusimamani, Olga Leuner
Summary: This study assessed the suitability of ten commonly used reference genes as optimal reference genes in M. officinalis under different abiotic stress conditions and plant organs. The combination of EF-1 alpha and GAPDH was recommended as the best reference genes for gene expression studies in M. officinalis.
Review
Biochemistry & Molecular Biology
Yanggu Shi, Jindong Shang
Summary: Circular RNAs are covalently circularized RNA loops with unique biochemical properties. Their biological functions and clinical indications continue to be discovered. They are increasingly being used as biomarkers due to their unusual specificities and stable circular form. Profiling circRNA expression is crucial in advancing circRNA research and we will review the use of circRNA microarrays in biological and clinical labs, sharing valuable experiences and significant findings.
Article
Chemistry, Analytical
M. G. Rizzo, S. Carnazza, L. M. De Plano, D. Franco, M. S. Nicol, V Zammuto, S. Petralia, Giovanna Calabrese, C. Gugliandolo, S. Conoci, S. P. P. Guglielmino
Summary: The study developed a new method for rapid and sensitive detection of bacteria in blood using engineered M13 phages and PCR in a miniaturized silicon microchip, providing a fast and efficient diagnosis approach.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Obstetrics & Gynecology
Francieli Maria Vigo, Renato Fraietta, Fernanda Rodrigues, Cristina Carvalho, Tatiana C. S. Bonetti, Eduardo L. A. Motta
Summary: This study investigated the role of miRNA in ovarian follicular development in patients undergoing different ovarian stimulation protocols, revealing differential expression of miRNA between the FSH plus LH group and FSH group, mainly related to estrogen signaling, oocyte meiosis, and pluripotent cell regulation. The results suggest that overexpression of miRNA in the FSH plus LH group is consistent with the independent and fundamental role of LH in folliculogenesis, leading to a distinct molecular response between groups.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Article
Biochemistry & Molecular Biology
Nadine de Godoy Torso, Julia Coelho Franca Quintanilha, Maria Aparecida Cursino, Eder de Carvalho Pincinato, Carmen Silvia Passos Lima, Patricia Moriel
Summary: This study aimed to determine the most adequate endogenous normalizer for urinary miRNA relative quantification in head and neck cancer patients treated with cisplatin chemoradiotherapy. RNA-Seq was performed to identify differentially expressed miRNAs between groups with and without cisplatin nephrotoxicity. Four miRNAs were selected as candidate endogenous normalizers and validated by qRT-PCR in a cohort of 49 patients, with hsa-miR-875-5p shown to be an adequate normalizer due to its invariant expression between the two groups.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Edward A. Mead, Nadia Boulghassoul-Pietrzykowska, Yongping Wang, Onaiza Anees, Noah S. Kinstlinger, Maximillian Lee, Shireen Hamza, Yaping Feng, Andrzej Z. Pietrzykowski
Summary: Alcohol Use Disorder (AUD) is a common mental disorder worldwide, and finding a reliable, cheap, and non-invasive biomarker for alcohol consumption is necessary. microRNAs can be detected in saliva and their expression is regulated by environmental factors. Analysis of microRNA expression in saliva of heavy alcohol abusers showed significant changes related to impaired immune system and development of epithelial cancer. Therefore, profiling microRNAs in saliva can serve as a non-invasive biomarker for monitoring alcohol abuse, alcohol-related inflammation, and early detection of cancer.
FRONTIERS IN GENETICS
(2022)
Article
Chemistry, Multidisciplinary
Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu
Summary: DNA quantification is crucial in life sciences research, and real-time MDA technology can provide template amount information during whole-genome amplification with a very low detection limit, offering a promising tool to quantify entire DNA without sacrificing sample completeness or introducing redundant steps.
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Youngseop Lee, Eun-Sil Yu, Hamin Na, Minhee Kang, Hee Jae Huh, Ki-Hun Jeong
Summary: The rapid and accurate molecular diagnostics are crucial in the face of pandemic diseases, and the nanoplasmonic PCR provides a new solution for rapid and quantitative molecular diagnostics at point-of-care level.
Article
Biochemistry & Molecular Biology
Li Deng, Wei Qing, Shuang Lai, Jiajun Zheng, Cong Liu, Hao Huang, Pairan Peng, Yandong Mu
Summary: This study analyzed the impact of grooved porous hydroxyapatite scaffold (HAG) on the osteogenic differentiation of human placenta-derived mesenchymal stem cells (hPMSCs) from the perspective of miRNA differential expression. The results revealed differentially expressed miRNAs and their potential target genes associated with osteogenic differentiation, highlighting the role of HAG in promoting osteogenesis.
DNA AND CELL BIOLOGY
(2022)
Article
Chemistry, Analytical
Liang Gong, Siyue Yao, Yidong He, Chengliang Liu
Summary: A new qPCR data analysis method called AERKM is proposed to achieve more accurate results by introducing amplification efficiency (AE) for error correction. It has been verified to have higher precision and stability in 5-point 10-fold gradient qPCR tests of 63 genes.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Zhiwei Chen, Nigel G. Halford, Chenghong Liu
Summary: Real-time quantitative PCR is a technique for measuring the content of a specific nucleic acid sequence in a sample, and it can be divided into absolute and relative quantitative methods. Relative quantification is mainly used for gene expression in functional genomics and transcriptome studies. However, to accurately use this technology, key points such as designing specific primers, selecting appropriate reference genes, and performing scientific calculations and statistics are crucial. This article proposes a workflow for relative quantitative PCR and introduces relevant points to help beginners better understand and utilize this technology.
Article
Biology
Anastasia O. Smirnova, Anna M. Miroshnichenkova, Yulia Olshanskaya, Michael A. Maschan, Yuri B. Lebedev, Dmitriy M. Chudakov, Ilgar Z. Mamedov, Alexander Komkov
Summary: High-throughput sequencing of adaptive immune receptor repertoires is valuable for studying adaptive immunity, but accurately detecting and correcting clone frequency discrepancies remains challenging. This study identified an anomaly in the ratio between read count and clone count-based frequencies of non-functional clonotypes in multiplex PCR-based immune repertoires. They developed a quantitative measure called Over Amplification Rate (OAR) to evaluate and correct V and J gene frequency bias caused by multiplex PCR. The iROAR software was successfully tested and shown to increase the accuracy and consistency of repertoire reconstruction for comparative analysis.
Article
Immunology
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su
Summary: This study developed a droplet digital PCR (ddPCR) assay for accurate and sensitive detection and quantification of Verticillium dahliae and V. longisporum, showing that ddPCR is more sensitive and accurate than qPCR for quantifying trace amounts of these pathogens in field samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Medicine, Research & Experimental
Gergely Buglyo, Zsofia Magyar, Eva Romicsne Gorbe, Rita Banusz, Monika Csoka, Tamas Micsik, Marta Mezei, Jaxi Ayman Shawky Yani, Peter Varga, Zoltan Sapi, Balint Nagy
Summary: This study revealed that miR-194-5p was downregulated in all Wilms' tumor samples, while miR-184 and miR-203a were also underexpressed in most cases. Additionally, RNA extraction from FFPE samples provided sufficient quantity and quality of miRNA for analysis, highlighting the relevance of pathological archives as a source for future miRNA studies.
MEDICAL SCIENCE MONITOR
(2021)
Article
Otorhinolaryngology
Kendrea L. (Focht) Garand, Lindsey Culp, Bin Wang, Kate Davidson, Bonnie Martin-Harris
ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY
(2020)
Letter
Respiratory System
Andrew C. Berry, Nicholas A. Berry, Bin Wang, Madhuri S. Mulekar, Anne Melvin, Richard J. Battiola, Frederick K. Bulacan, Bruce B. Berry
CLINICAL RESPIRATORY JOURNAL
(2020)
Article
Multidisciplinary Sciences
Bin Wang
Article
Audiology & Speech-Language Pathology
Erin M. Milford, Bin Wang, Kimberly Smith, Dahye Choi, Bonnie Martin-Harris, Kendrea L. (Focht) Garand
AMERICAN JOURNAL OF SPEECH-LANGUAGE PATHOLOGY
(2020)
Article
Multidisciplinary Sciences
Chen Ge, Shu-Guang Zhang, Bin Wang
Review
Chemistry, Multidisciplinary
Ruixia Ma, Bin Yi, Adam I. Riker, Yaguang Xi
ACTA PHARMACOLOGICA SINICA
(2020)
Review
Chemistry, Multidisciplinary
Xiaolin Liu, Ruixia Ma, Bin Yi, Adam I. Riker, Yaguang Xi
Summary: miRNAs play crucial roles in gastric cancer, acting as both tumor suppressors and oncogenes with their full roles still not fully understood. Recent studies focus on miRNAs' regulation of gastric tumor cell proliferation, apoptosis, and cell cycle, as well as their impact on epithelial-mesenchymal transition, tumor microenvironment, and chemoresistance in GC cells, contributing to the understanding of GC etiology and development of new treatment strategies.
ACTA PHARMACOLOGICA SINICA
(2021)
Article
Biochemistry & Molecular Biology
Zhipin Liang, Zhiqiang Qin, Adam Riker, Yaguang Xi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Oncology
Bin Yi, Hao Cheng, Dorota Wyczechowska, Qingzhao Yu, Li Li, Augusto C. Ochoa, Adam Riker, Yaguang Xi
Summary: Sulindac can downregulate PD-L1 by blocking NF-kB signaling, leading to increased availability of PD-L1 Ab and potentially improving the overall efficacy of anti-PD-L1 therapy. Combination therapy showed significant inhibitory effects on tumor growth in pMMR colorectal cancer, along with increased infiltration of CD8(+) T lymphocytes in tumor tissues.
MOLECULAR CANCER THERAPEUTICS
(2021)
Article
Zoology
N. Katlein, M. Ray, A. Wilkinson, J. Claude, M. Kiskowski, B. Wang, S. Glaberman, Y. Chiari
Summary: Animals are exposed to various visual stimuli that affect their perception and interaction with the environment. This study examines whether color influences an animal's response to objects and whether this influence varies depending on the type of object. The results indicate that color does not change the response to objects. Additionally, the duration of interaction with images is longer for diurnal species compared to nocturnal species, but this is also independent of coloration. Gender differences were not observed within or between species. These findings are important for understanding which visual stimuli elicit responses in animals.
JOURNAL OF ZOOLOGY
(2022)
Article
Cell Biology
Alexander B. Coley, Ashlyn N. Stahly, Mohan Kasukurthi, Addison A. Barchie, Sam B. Hutcheson, Dominika Houserova, Yulong Huang, Brianna C. Watters, Valeria M. King, Meghan A. Dean, Justin T. Roberts, Jeffrey D. DeMeis, Krisha Amin, Cameron H. McInnis, Noel L. Godang, Ryan M. Wright, David F. Haider, Neha B. Piracha, Cana L. Brown, Zohaib M. Ijaz, Shengyu Li, Yaguang Xi, Oliver G. McDonald, Jingshan Huang, Glen M. Borchert
Summary: This study identified differentially expressed snoRNA fragments in prostate cancer patients and found that two specific fragments, sdRNA-D19b and -A24, play important roles in promoting proliferation, migration, and chemotherapeutic resistance in prostate cancer. The targets of sdRNA-D19b and sdRNA-A24 were identified as tumor suppressor genes CD44 and CDK12, respectively.
Article
Cell Biology
Ritu Arora, Jin-Hwan Kim, Ayechew A. Getu, Anusha Angajala, Yih-Lin Chen, Bin Wang, Andrea G. Kahn, Hong Chen, Latif Reshi, Jianrong Lu, Wenling Zhang, Ming Zhou, Ming Tan
Summary: The highly expressed MST4 gene in breast cancer promotes cell growth, migration, invasion, and epithelial-mesenchymal transition (EMT). It is associated with cancer stage, lymph node metastasis, and poor overall survival.
Article
Biochemistry & Molecular Biology
Hongyou Zhao, Bin Yi, Zhipin Liang, Ches'Nique Phillips, Hui-Yi Lin, Adam Riker, Yaguang Xi
Summary: Sulindac inhibits colorectal cancer cell cycle progression through up-regulation of Cyclin G2 and down-regulation of MiR-182, in turn enhancing FOXO3a transcriptional activity. The expression levels of MiR-182/FOXO3a/Cyclin G2 could potentially serve as predictive markers for tumor progression in colorectal cancer.
Article
Public, Environmental & Occupational Health
A. C. Berry, B. D. Cash, B. Wang, M. S. Mulekar, A. B. Van Haneghan, K. Yuquimpo, A. Swaney, M. C. Marshall, W. K. Green
EPIDEMIOLOGY AND INFECTION
(2019)
Article
Oncology
Mohd Khushman, Girijesh Kumar Patel, Javier Ariel Launrini, Arun Bhardwaj, Kelly Roveda, Robert Donnell, Kelley Sherling, Brittany Case, Arthur E. Frankel, Sachin Pai, William Taylor, Marcus Chuan Beng Tan, Meir Mizrahi, Cindy Nelson, Mary Wyatt, Mary Patton, Steven McClellan, Seema Singh, Bin Wang, Ajay P. Singh
JOURNAL OF GASTROINTESTINAL ONCOLOGY
(2019)