Article
Microbiology
Hasan Tukenmez, Pardeep Singh, Souvik Sarkar, Melike Cakir, Ana H. Oliveira, Cecilia Lindgren, Karolis Vaitkevicius, Mari Bonde, A. Elisabeth Sauer-Eriksson, Fredrik Almqvist, Jorgen Johansson
Summary: Bacteria resistant to antibiotics are a major problem, requiring the development of new antimicrobial drugs. In this study, the effectiveness of a new compound, PS900, in inhibiting virulence factor expression in Listeria monocytogenes was demonstrated. Furthermore, PS900 was found to potentiate the bactericidal effects of cholic acid and osmolytes, making it a promising candidate for future antibacterial drug design.
Article
Immunology
David R. Mains, Samuel J. Eallonardo, Nancy E. Freitag
Summary: The study shows that L. monocytogenes prfA* mutants exhibit significantly enhanced resistance to oxidative stress. Furthermore, transposon mutagenesis led to the identification of three novel gene targets required for full oxidative stress resistance only in the context of PrfA activation. Additionally, inactivation of cbpA and lmo0779 in a prfA* background resulted in reduced virulence in infected mice.
INFECTION AND IMMUNITY
(2021)
Article
Immunology
Josephine Moran, Liam Feltham, James Bagnall, Marie Goldrick, Elizabeth Lord, Catherine Nettleton, David G. Spiller, Ian Roberts, Pawel Paszek
Summary: The paper investigates the real-time interaction between the food-borne pathogen L. monocytogenes and host macrophages, revealing heterogeneous outcomes of infection and the impact of bacterial quantity on macrophage phagocytosis ability. It also highlights the role of population-level PrfA-mediated activity in regulating host-pathogen interactions.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Lakshmi Narayanan, Ozan Ozdemir, Navatha Alugubelly, Reshma Ramachandran, Michelle Banes, Mark Lawrence, Hossam Abdelhamed
Summary: The study used Tn mutagenesis to identify genes and pathways affecting the growth and fitness of Listeria monocytogenes in minimal medium. The findings revealed that biosynthesis of purines, pyrimidines, ATP, and branched-chain fatty acids plays an important role in the pathogenesis of L. monocytogenes.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Microbiology
Thulani Sibanda, Elna M. Buys
Summary: This review explores the molecular basis of the connection between stress tolerance responses and pathogenesis of L. monocytogenes. It is found that stress-induced adaptive responses in food can protect the pathogen in the gastrointestinal tract and switch its gene expression from avirulent to virulent state. The review also discusses the overlapping and interlinked regulatory networks for SigB and PrfA.
Review
Biochemistry & Molecular Biology
Fantin Mesny, Stephane Hacquard, Bart P. H. J. Thomma
Summary: Plants interact with a diverse array of microorganisms that affect their growth and resilience, leading them to be considered as plant holobionts rather than singular organisms. The assembly of above- and belowground microbiota in a plant holobiont is influenced by host, microbial, and environmental factors. Plants activate immune signaling upon perceiving microorganisms, which then modulate the composition of the plant microbiota through the secretion of factors. Metabolic interdependencies and antagonism between microbes are also important for shaping community assemblies. Overall, these complex interactions have evolved and play a crucial role in promoting the survival and fitness of plants and their associated microorganisms as holobionts. The co-evolution of these interactions within holobionts ultimately influences plant performance.
Article
Public, Environmental & Occupational Health
Sara Kazemi Rad, Mehdi Assmar, Mirsasan Mirpour, Mohamad Reza Razavi
Summary: This study aimed to isolate L. monocytogenes bacteria from vaginal samples of pregnant women and evaluate the effects of chitosan nanoparticles on them. The results showed that L. monocytogenes could be a causative agent of abortion in pregnant women and nano chitosan exhibited acceptable antibacterial activity against L. monocytogenes.
IRANIAN JOURNAL OF PUBLIC HEALTH
(2022)
Article
Immunology
Xiaochun Xia, Yuan Chen, Jianming Xu, Chundong Yu, Wenbo Chen
Summary: SRC-3 deficiency in mice increases resistance to Listeria monocytogenes infection, resulting in reduced mortality, bacterial load, weight loss, proinflammatory cytokines, and tissue damage compared to wild-type mice. This is achieved through increased ROS production and decreased lymphocyte apoptosis, potentially involving the downregulation of negative regulators of ROS and interferon-13.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Chemistry, Analytical
Claudia Maciel, Nadia F. D. Silva, Paula Teixeira, Julia M. C. S. Magalhaes
Summary: This study proposes a novel integrated system using loop-mediated isothermal amplification (LAMP) and a bacteriophage P100-magnetic platform, coupled with endpoint electrochemical techniques, for rapid detection of L. monocytogenes. Through validation in pasteurized milk spiked with L. monocytogenes, the system demonstrated outstanding performance and robustness, providing a rapid, portable, and cost-efficient detection scheme for decentralized on-field application.
Review
Engineering, Electrical & Electronic
Zhiwei Li, Anjun Zhu, Xiumei Ma, Shiming Liang, Zhengyou Zhu, Song Xiao
Summary: Acetoin is a metabolic biomarker gas of Listeria monocytogenes. The detection of acetoin biomarker can indirectly monitor Listeria monocytogenes, which has gained attention in the gas sensing field. Research on acetoin gas sensors is still in its early stages and there is a need to optimize the sensing materials and clarify the sensing mechanism. This mini-review presents recent advances in acetoin gas sensors based on semiconductor metal oxides (SMOs), explains the sensing mechanism, and discusses research shortcomings and future prospects.
MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING
(2023)
Review
Biochemistry & Molecular Biology
Saverio Marchi, Gianluca Morroni, Paolo Pinton, Lorenzo Galluzzi
Summary: Mitochondria play a vital role in maintaining cellular and organismal homeostasis, and various pathogenic bacteria have developed strategies to control the mitochondrial compartment of host cells.
TRENDS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ahmed Gaballa, Sriya Sunil, Etienne Doll, Sarah I. Murphy, Tyler Bechtel, Veronica Guariglia-Oropeza, Martin Wiedmann
Summary: The abundance of metal ions and the levels of intracellular and extracellular iron can modulate the expression of virulence gene hly in Listeria monocytogenes. Activated charcoal reduces the intracellular labile iron pool and synergistically contributes to the expression of PrfA-regulated genes with metal depletion and exogenously added glutathione. Metal ion abundance plays a role in regulating the expression of PrfA-regulated virulence genes in L. monocytogenes.
Article
Multidisciplinary Sciences
Gina M. Borgo, Thomas P. Burke, Cuong J. Tran, Nicholas T. N. Lo, Patrik Engstrom, Matthew D. Welch
Summary: This study identified a phospholipase enzyme in Rickettsia bacteria that plays an important role in the invasion and spread of infection by helping the bacteria escape from host cell vacuoles, preventing targeting by autophagy, and promoting bacterial motility and spread to other cells.
NATURE COMMUNICATIONS
(2022)
Review
Microbiology
Joseph J. Wanford, Charlotte Odendall
Summary: Calcium ions (Ca2+) play a crucial role in modulating innate immunity in multicellular organisms, as they regulate cell physiological responses. However, during infection with host-adapted pathogens, Ca2+ signals are often atypical due to the activities of virulence factors, with varied consequences for both the pathogen and the host cell.
CURRENT OPINION IN MICROBIOLOGY
(2023)
Review
Microbiology
Jeremy Verbeke, Xavier De Bolle, Thierry Arnould
Summary: Mitochondria play essential roles in cellular functions and can serve as a target for invading pathogens. The modulation of mitochondrial functions by bacterial pathogens remains poorly understood, particularly in the context of mitophagy. Both the host and the pathogen can potentially initiate mitophagy for different purposes during bacterial infection.