Article
Multidisciplinary Sciences
Linda Su-Feher, Anna N. Rubin, Shanni N. Silberberg, Rinaldo Catta-Preta, Kenneth J. Lim, Athena R. Ypsilanti, Iva Zdilar, Christopher S. McGinnis, Gabriel L. McKinsey, Thomas E. Rubino, Michael J. Hawrylycz, Carol Thompson, Zev J. Gartner, Luis Puelles, Hongkui Zeng, John L. R. Rubenstein, Alex S. Nord
Summary: Enhancers play a crucial role in driving cell fate specification in the developing brain. In this study, the researchers used enhancer labeling and single-cell RNA sequencing to map and distinguish different neuronal lineages in the mouse embryonic brain. The results showed that combining enhancer activity profiling with scRNA-seq improved the resolution of regional and developmental populations and identified specific neuronal populations derived from different brain regions. This study highlights the importance of enhancers and the power of scRNA-seq in understanding the complex paths of neuronal specification in mouse brain development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Cell Biology
Ye Xie, Bo Chen
Summary: This article discusses the regenerative potential of Muller glia (MG) in the mouse retina, focusing on the progress made in the de novo regeneration of retinal neurons through the reprogramming of MG.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Fuyun Bian, Marwa Daghsni, Fangfang Lu, Silvia Liu, Jeffrey M. Gross, Issam Aldiri
Summary: The transcription factor Vsx2 plays a critical role in retinal proliferation and bipolar cell differentiation. It binds to and transactivates its enhancer in association with Pax6. Vsx2 deletion in mice results in specific abnormalities in retinal proliferation and bipolar cell differentiation. Vsx2 occupies cis-regulatory elements nearby genes associated with photoreceptor differentiation and homeostasis in both mouse and human retina. It can also suppress Otx2-dependent activation of the Prdm1 enhancer. These findings provide important insights into the mechanisms of Vsx2 engagement with gene regulatory networks in retinal development.
Article
Anatomy & Morphology
M. Madrigal, P. Martin, F. Lamus, J. M. Fernandez, A. Gato, MI. Alonso
Summary: The adult mouse hippocampal neurogenic niche is a complex structure that is not fully understood. Neural precursors have been found in various areas of the adult mouse hippocampus, including the Subependymal Zone, Dentate Migratory Stream, and hilus, and they display dynamic behavior compatible with neurogenesis. This suggests that the adult hippocampal niche is not restricted to the dentate gyrus subgranular layer.
Article
Biotechnology & Applied Microbiology
Gang Chen, Hong-Mei Qian, Jing Chen, Jie Wang, Ji-Tian Guan, Zai-Long Chi
Summary: The study systematically screened key non-coding RNAs in retinal neurogenesis through whole-transcriptome sequencing. The validated non-coding RNAs and their circular RNA/long non-coding RNA-microRNA-messenger RNA network involve neurotransmitter transport and multicellular organism growth during retinal development.
Article
Developmental Biology
Bianca M. Lupan, Rachel A. Solecki, Camila M. Musso, Fernando C. Alsina, Debra L. Silver
Summary: This study demonstrates that EIF4A3 promotes cortical development by controlling progenitor mitosis, cell fate, and survival. The lack of EIF4A3 leads to cell death and impaired neurogenesis. The research also reveals new mechanisms of neurogenesis mediated by the exon junction complex (EJC).
Article
Developmental Biology
Bernadett Bosze, Julissa Suarez-Navarro, Abdul Soofi, James D. Lauderdale, Gregory R. Dressler, Nadean L. Brown
Summary: The development of eyes is precisely controlled by genetic programs, with Pax genes playing a key role. Mutations in Pax2 can lead to ocular coloboma due to incomplete closure of the ventral optic fissure. Studies have shown the importance of Pax2 in maintaining boundaries between tissues like the neural retina.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Angelo Harlan De Crescenzo, Alexios A. Panoutsopoulos, Lyvin Tat, Zachary Schaaf, Shailaja Racherla, Lyle Henderson, Kit-Yi Leung, Nicholas D. E. Greene, Ralph Green, Konstantinos S. Zarbalis
Summary: Folate is crucial during pregnancy, with both deficiency and excess potentially leading to neurodevelopmental abnormalities in offspring and behavioral changes; research suggests that such interventions alter the folate cycle, delaying prenatal cerebral cortical neurogenesis.
Article
Developmental Biology
Huanqing Zhang, Pei Zhuang, Ryan M. Welchko, Manhong Dai, Fan Meng, David L. Turner
Summary: This study reveals that miR-216b is highly expressed in postmitotic retinal amacrine cells in the mouse retina and its expression in the retina is partly regulated by the transcription factor Ptf1a. Ectopic expression of miR-216b leads to increased amacrine cell formation and decreased bipolar neuron formation in the developing retina. The researchers identify Foxn3 mRNA as a target of miR-216b and show that Foxn3 is a novel regulator of interneuron formation in the developing retina.
Article
Biochemistry & Molecular Biology
Jana Pereckova, Michaela Pekarova, Nikoletta Szamecova, Zuzana Hoferova, Kristyna Kamarytova, Martin Falk, Tomas Perecko
Summary: NO2-OA modulates the pluripotency of mESC by reducing STAT3 phosphorylation, attenuating cardiac differentiation, and directing mESC into neural fate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Nishtha Ranawat, Ichiro Masai
Summary: Microglia are brain-resident macrophages that colonize specific brain regions during development, such as the retina. They use blood vessels as pathways and depend on neuronal differentiation for proper migration. Factors like IL34 also play a role in microglial precursor colonization of the retina.
Article
Biology
Christiane Voufo, Andy Quaen Chen, Benjamin E. Smith, Rongshan Yan, Marla B. Feller, Alexandre Tiriac
Summary: Spontaneous activity plays an important role in the development of neural systems. This study focuses on embryonic retinal waves, specifically stage 1 waves, and reveals their spatiotemporal properties and underlying mechanisms. The results suggest that stage 1 waves are mediated by a circuit involving specific types of receptors and gap junctions. The impact of stage 1 waves on retinal development, particularly on the distribution of a subtype of retinal ganglion cells, is also investigated.
Review
Genetics & Heredity
Keiichi Ishihara
Summary: Down syndrome, also known as trisomy 21, is the most frequent genetic cause of intellectual disability. Delayed brain development may be involved in DS intellectual disability. Using Cre-loxP-mediated chromosomal engineering can generate various mouse models for determining genotype-phenotype correlations.
Article
Multidisciplinary Sciences
Shang-Yin Chiang, Hsin-Chieh Wu, Shu-Yu Lin, Hsin-Yi Chen, Chia-Fang Wang, Nai-Hsing Yeh, Jou-Ho Shih, Yi-Shuian Huang, Hung-Chih Kuo, Shen-Ju Chou, Ruey-Hwa Chen
Summary: The study highlights the critical role of protein stabilization mechanisms in cortical development, showing that Usp11 deficiency impairs neuron production and migration, and disease-associated Usp11 mutant fails to support these processes.
Article
Cell & Tissue Engineering
Naoya Yuizumi, Yujin Harada, Takaaki Kuniya, Takehiko Sunabori, Masato Koike, Masaki Wakabayashi, Yasushi Ishihama, Yutaka Suzuki, Daichi Kawaguchi, Yukiko Gotoh
Summary: Lysosomes play a crucial role in regulating the differentiation of neural stem-progenitor cells in the embryonic brain, with TFEB and TFE3 potentially maintaining the undifferentiated state of NPCs through controlling lysosomal biosynthesis. The abundance of lysosomes is associated with cell division activity and the embryonic origin of adult NSCs. TFEB-AA overexpression suppresses neuronal differentiation and the cell cycle of neocortical NPCs.
Review
Biotechnology & Applied Microbiology
Ivana Trapani, Sandro Banfi, Francesca Simonelli, Enrico M. Surace, Alberto Auricchio
HUMAN GENE THERAPY
(2015)
Article
Biochemical Research Methods
Carlotta Lambertini, Domenico Ventrella, Francesca Barone, Nicolina Cristina Sorrentino, Francesco Dondi, Alessandro Fraldi, Massimo Giunti, Enrico Maria Surace, Maria Laura Bacci, Noemi Romagnoli
JOURNAL OF NEUROSCIENCE METHODS
(2015)
Article
Biotechnology & Applied Microbiology
Anna Manfredi, Elena Marrocco, Agostina Puppo, Giulia Cesi, Andrea Sommella, Michele Della Corte, Settimio Rossi, Massimo Giunti, Cheryl M. Craft, Maria Laura Bacci, Francesca Simonelli, Enrico M. Surace, Alberto Auricchio
HUMAN GENE THERAPY
(2013)
Article
Veterinary Sciences
Noemi Romagnoli, Domenico Ventrella, Massimo Giunti, Francesco Dondi, Nicolina C. Sorrentino, Alessandro Fraldi, Enrico M. Surace, Maria L. Bacci
LABORATORY ANIMALS
(2014)
Article
Multidisciplinary Sciences
Daniela Iaconis, Maria Monti, Mario Renda, Arianne van Koppen, Roberta Tammaro, Marco Chiaravalli, Flora Cozzolino, Paola Pignata, Claudia Crina, Piero Pucci, Alessandra Boletta, Vincenzo Belcastro, Rachel H. Giles, Enrico Maria Surace, Simone Gallo, Mario Pende, Brunella Franco
SCIENTIFIC REPORTS
(2017)
Article
Medicine, Research & Experimental
Alessia Indrieri, Sabrina Carrella, Alessia Romano, Alessandra Spaziano, Elena Marrocco, Erika Fernandez-Vizarra, Sara Barbato, Mariateresa Pizzo, Yulia Ezhova, Francesca M. Golia, Ludovica Ciampi, Roberta Tammaro, Jorge Henao-Mejia, Adam Williams, Richard A. Flavell, Elvira De Leonibus, Massimo Zeviani, Enrico M. Surace, Sandro Banfi, Brunella Franco
EMBO MOLECULAR MEDICINE
(2019)
Article
Cell Biology
Patrizia Tornabene, Ivana Trapani, Renato Minopoli, Miriam Centrulo, Mariangela Lupo, Sonia de Simone, Paola Tiberi, Fabio Dell'Aquila, Elena Marrocco, Carolina Iodice, Antonella Iuliano, Carlo Gesualdo, Settimio Rossi, Laura Giaquinto, Silvia Albert, Carel B. Hoyng, Elena Polishchuk, Frans P. M. Cremers, Enrico M. Surace, Francesca Simonelli, Maria A. De Matteis, Roman Polishchuk, Alberto Auricchio
SCIENCE TRANSLATIONAL MEDICINE
(2019)
Article
Medicine, Research & Experimental
Marianthi Karali, Irene Guadagnino, Elena Marrocco, Rossella De Cegli, Annamaria Carissimo, Mariateresa Pizzo, Simona Casarosa, Ivan Conte, Enrico Maria Surace, Sandro Banfi
MOLECULAR THERAPY-NUCLEIC ACIDS
(2020)
Article
Biochemistry & Molecular Biology
Federica Naso, Daniela Intartaglia, Danila Falanga, Chiara Soldati, Elena Polishchuk, Giuliana Giamundo, Paola Tiberi, Elena Marrocco, Paolo Scudieri, Chiara Di Malta, Ivana Trapani, Edoardo Nusco, Francesco Giuseppe Salierno, Enrico Maria Surace, Luis J. Galietta, Sandro Banfi, Alberto Auricchio, Andrea Ballabio, Diego Luis Medina, Ivan Conte
Article
Clinical Neurology
Robin Reynaud-Dulaurier, Giorgia Benegiamo, Elena Marrocco, Racha Al-Tannir, Enrico Maria Surace, Johan Auwerx, Michael Decressac
Article
Genetics & Heredity
Clarissa Patrizi, Manel Llado, Daniela Benati, Carolina Iodice, Elena Marrocco, Rosellina Guarascio, Enrico M. Surace, Michael E. Cheetham, Alberto Auricchio, Alessandra Recchia
Summary: RP is a progressive retinal degenerative disease causing blindness, and CRISPR/Cas9 can selectively target specific variants for gene editing to restore partial retinal function.
AMERICAN JOURNAL OF HUMAN GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Elena Marrocco, Rosa Maritato, Salvatore Botta, Marianna Esposito, Enrico Maria Surace
Summary: The synthetic transcriptional repressor ZF6-DB designed for treating retinitis pigmentosa does not disrupt murine retinal development while effectively blocking target gene expression. Delivery of ZF6-DB during retinal development in mice results in morphological and functional recovery of the retina in a model of retinitis pigmentosa. The study suggests the use of developmental transitions as a safe approach for retinal gene therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Patrizia Tornabene, Ivana Trapani, Miriam Centrulo, Elena Marrocco, Renato Minopoli, Mariangela Lupo, Carolina Iodice, Carlo Gesualdo, Francesca Simonelli, Enrico M. Surace, Alberto Auricchio
Summary: The study found that by optimizing inteins, the capacity of AAV vectors was expanded, improving the effectiveness of gene therapy, and success was achieved in a mouse model of Stargardt disease. This provides a new approach for treating STGD1 and other diseases requiring the transfer of large genes.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Medicine, Research & Experimental
Salvatore Botta, Nicola de Prisco, Elena Marrocco, Mario Renda, Martina Sofia, Fabiola Curion, Maria Laura Bacci, Domenico Ventrella, Cathal Wilson, Carlo Gesualdo, Settimio Rossi, Francesca Simonelli, Enrico Maria Surace
Article
Biology
Salvatore Botta, Elena Marrocco, Nicola de Prisco, Fabiola Curion, Mario Renda, Martina Sofia, Mariangela Lupo, Annamaria Carissimo, Maria Laura Bacci, Carlo Gesualdo, Settimio Rossi, Francesca Simonelli, Enrico Maria Surace