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Medicine, General & Internal
Deborah Delbue, Lydia Lebenheim, Danielle Cardoso-Silva, Violaine Dony, Susanne M. Krug, Jan F. Richter, Subhakankha Manna, Melba Munoz, Kerstin Wolk, Claudia Heldt, Markus M. Heimesaat, Robert Sabat, Britta Siegmund, Michael Schumann
Summary: IL-22 impairs intestinal epithelial cell barrier by inducing EMT, causing defects in epithelial cell polarity and increasing cell motility and cell invasion. IL-22 modulates TJ protein expression and mediates tight junctional (TJal) barrier defects via ERK pathway.
FRONTIERS IN MEDICINE
(2021)
Review
Oncology
Anne Liu, Yanyu Zhu, Weiping Chen, Glenn Merlino, Yanlin Yu
Summary: PTEN is a critical tumor suppressor gene with multifunctional phosphatase activity, and its protein-phosphatase activity plays a crucial role in tumor progression, although its mechanism is not fully understood.
Article
Biology
Maria Danielle Sallee, Melissa A. Pickett, Jessica L. Feldman
Summary: Research on developing Caenorhabditis elegans intestines reveals the importance of the apical PAR complex in maintaining intestinal epithelial integrity and constructing a functional tube. Depletion of PAR-6, PKC-3, and CDC-42 regulators leads to gaps in apical proteins, hindering food passage and causing larval death.
Review
Biochemistry & Molecular Biology
Sudha B. Singh, Henry C. Lin
Summary: Intestinal alkaline phosphatase (IAP) is a multifunctional protein that plays a protective role in the gut, with defects leading to various gastrointestinal disorders. Studies using exogenous IAP supplementation and cellular overexpression have helped to elucidate its mechanisms in innate immunity. Furthermore, IAP has been shown to induce autophagy, highlighting its significant impact on gut health.
Article
Biochemistry & Molecular Biology
Xiaobin Wen, Ruqing Zhong, Guoqi Dang, Bing Xia, Weida Wu, Shanlong Tang, Lixin Tang, Lei Liu, Zhengqun Liu, Liang Chen, Hongfu Zhang
Summary: The study suggests that pectin enhances intestinal integrity and barrier function by altering intestinal microbiota composition and their metabolites, which subsequently alleviates intestinal injury and improves the growth performance of piglets.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
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Genetics & Heredity
Pan Xu, Montserrat Elizalde, Ad Masclee, Marieke Pierik, Daisy Jonkers
Summary: This study demonstrates that prednisolone has a beneficial effect on the epithelial barrier of CD patient-derived intestinal organoids and can restore barrier dysfunction induced by cytokines.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Gastroenterology & Hepatology
Chiara Muzzi, Norika Watanabe, Eric Twomey, Garrit K. Meers, Holger M. Reichardt, Hanibal Bohnenberger, Sybille D. Reichardt
Summary: This study highlights the important role of intestinal epithelial cells as targets of glucocorticoid action in inflammatory bowel disease, and suggests that the effectiveness of controlling colitis directly impacts the progression of colorectal cancer.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Gastroenterology & Hepatology
Luz del Carmen Martinez-Sanchez, Phuong Anh Ngo, Rashmita Pradhan, Lukas-Sebastian Becker, David Boehringer, Despina Soteriou, Marketa Kubankova, Christine Schweitzer, Tatyana Koch, Veronika Thonn, Lena Erkert, Iris Stolzer, Claudia Guenther, Christoph Becker, Benno Weigmann, Monika Klewer, Christoph Daniel, Kerstin Amann, Stefan Tenzer, Raja Atreya, Martin Bergo, Cord Brakebusch, Alastair J. M. Watson, Jochen Guck, Ben Fabry, Imke Atreya, Markus F. Neurath, Rocio Lopez-Posadas
Summary: Abnormal cell shedding is associated with intestinal barrier dysfunction and inflammatory bowel diseases. Deletion of PGGTase and RAC1 in intestinal epithelial cells leads to cell overcrowding and epithelial leakage, resulting in chronic intestinal inflammation. RAC1 plays a crucial role in regulating cytoskeletal dynamics, cell mechanics, and intestinal cell shedding.
Article
Biotechnology & Applied Microbiology
Panagiota Kakni, Carmen Lopez-Iglesias, Roman Truckenmueller, Pamela Habibovic, Stefan Giselbrecht
Summary: The inner surface of the intestine is a dynamic system. Researchers have developed intestinal organoids that mimic intestinal architecture and cell composition. However, the current models limit the study of the apical side of the epithelium. This study presents a novel intestinal organoid model with reversed polarity, which provides a new research tool for studying nutrient/drug uptake, metabolism, and host-microbiome/pathogen interactions.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Cell Biology
Elisabeth G. Foerster, Tapas Mukherjee, Liliane Cabral-Fernandes, Juliana D. B. Rocha, Stephen E. Girardin, Dana J. Philpott
Summary: Autophagy is a crucial cellular process for recycling organelles and protein aggregates, as well as destroying intracellular pathogens. It plays a key role in the maintenance and repair of intestinal epithelium, supporting intestinal barrier function and influencing the development of intestinal disease.
Article
Gastroenterology & Hepatology
Hajar Hazime, G. Michelle Ducasa, Ana M. Santander, Nivis Brito, Eddy E. Gonzalez, Yuguang Ban, Jonathan Kaunitz, Yasutada Akiba, Irina Fernandez, Juan F. Burgueno, Maria T. Abreu
Summary: The inactivity of intestinal epithelial DUOX2 is linked to alterations in the gut microbiome and contributes to the development of Metabolic Syndrome (MetS).
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Veterinary Sciences
Ruibiao Wang, Yuheng Zhang, Jingbo Zhen, Jinpeng Zhang, Zixuan Pang, Xuewei Song, Lihao Lin, Feng Sun, Yixin Lu
Summary: This study explored the effect of exosomes derived from Trichinella spiralis infective larvae (TsExos) on the barrier function of porcine small intestinal epithelial cells (IPEC-J2). The results showed that TsExos ingestion decreased cell viability, increased FITC-dextran content, lactate dehydrogenase and reactive oxygen species levels. TsExos also induced apoptosis, nuclear pyknosis, and nuclear rupture. In addition, TsExos disrupted tight junctions and altered gene expression.
VETERINARY RESEARCH
(2022)
Article
Medicine, General & Internal
Sisi Huang, Sheng Zhang, Limin Chen, Xiaojun Pan, Zhenliang Wen, Yizhu Chen, Lidi Zhang, Jiao Liu, Dechang Chen
Summary: This study establishes an organoid-based intestinal injury model for sepsis, providing a platform for studying the effects of lipopolysaccharide-induced intestinal injury resembling sepsis. The model allows for exploration of immune-associated mechanisms and effective drug screening.
CHINESE MEDICAL JOURNAL
(2022)
Article
Biology
Katharina M. C. Klee, Michael W. Hess, Michael Lohmueller, Sebastian Herzog, Kristian Pfaller, Thomas Mueller, Georg F. Vogel, Lukas A. Huber, Michel Bagnat
Summary: In this study, we conducted a genome-wide CRISPR/Cas9 screen combined with FACS to identify novel regulators of epithelial polarization and protein secretion in human polarized epithelial cells. Through high-resolution microscopy, we confirmed the role of TM9SF4, anoctamin 8, and ARHGAP33 in epithelial polarization and apical cargo secretion. This study provides a powerful tool for studying these processes and offers a dataset for the investigation of novel congenital diseases associated with epithelial polarization and polarized transport.
Review
Cell Biology
Katlynn Bugda Gwilt, Jay R. Thiagarajah
Summary: The development of cell polarity in epithelia is crucial for tissue morphogenesis and transport between the environment and the underlying tissue. Membrane lipids, especially phosphoinositide species, play a primary role in generating epithelial polarity by influencing membrane lipid distribution and protein localization and vesicular trafficking.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
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Biochemistry & Molecular Biology
Y. Gui, M. Yeganeh, Y-C Donates, W-S Tobelaim, W. Chababi, M. Mayhue, A. Yoshimura, S. Ramanathan, C. Saucier, S. Ilangumaran
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Multidisciplinary Sciences
William S. Tobelaim, Claudia Beaurivage, Audrey Champagne, Veronique Pomerleau, Aline Simoneau, Walid Chababi, Mehdi Yeganeh, Philippe Thibault, Roscoe Klinck, Julie C. Carrier, Gerardo Ferbeyre, Subburaj Ilangumaran, Caroline Saucier
SCIENTIFIC REPORTS
(2015)
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Biochemistry & Molecular Biology
Melissa Landry, Veronique Pomerleau, Caroline Saucier
BIOCHEMICAL JOURNAL
(2016)
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Oncology
Gina Bouchard, Helene Therriault, Sameh Geha, Yves Berube-Lauziere, Rachel Bujold, Caroline Saucier, Benoit Paquette
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Biochemistry & Molecular Biology
Claudia Beaurivage, Audrey Champagne, William S. Tobelaim, Veronique Pomerleau, Alfredo Menendez, Caroline Saucier
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Biochemistry & Molecular Biology
M. Yeganeh, Y. Gui, R. Kandhi, D. Bobbala, W-S Tobelaim, C. Saucier, A. Yoshimura, G. Ferbeyre, S. Ramanathan, S. Ilangumaran
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Oncology
A. Villalobos-Hernandez, D. Bobbala, R. Kandhi, M. G. M. Khan, M. Mayhue, C. M. Dubois, G. Ferbeyre, C. Saucier, S. Ramanathan, S. Ilangumaran
PROSTATE CANCER AND PROSTATIC DISEASES
(2017)
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Oncology
Galaxia M. Rodriguez, Diwakar Bobbala, Daniel Serrano, Marian Mayhue, Audrey Champagne, Caroline Saucier, Viktor Steimle, Thomas A. Kufer, Alfredo Menendez, Sheela Ramanathan, Subburaj Ilangumaran
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Biology
Gina Bouchard, Helene Therriault, Rachel Bujold, Caroline Saucier, Benoit Paquette
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2017)
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Gastroenterology & Hepatology
Yirui Gui, Md Gulam Musawwir Khan, Diwakar Bobbala, Claire Dubois, Sheela Ramanathan, Caroline Saucier, Subburaj Ilangumaran
WORLD JOURNAL OF GASTROENTEROLOGY
(2017)
Editorial Material
Cell Biology
Catherine M. Duclos, Audrey Champagne, Julie C. Carrier, Caroline Saucier, Christine L. Lavoie, Jean-Bernard Denault
CELL DEATH & DISEASE
(2017)
Meeting Abstract
Oncology
Catherine M. Duclos, Audrey Champagne, Julie C. Carrier, Caroline Saucier, Christine L. Lavoie, Jean-Bernard Denault
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Oncology
Martin Chevrier, Diwakar Bobbala, Alberto Villalobos-Hernandez, Md Gulam Musawwir Khan, Sheela Ramanathan, Caroline Saucier, Gerardo Ferbeyre, Sameh Geha, Subburaj Ilangumaran
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Oncology
Gina Bouchard, Helene Therriault, Sameh Geha, Rachel Bujold, Caroline Saucier, Benoit Paquette
BRITISH JOURNAL OF CANCER
(2017)
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Cell Biology
Catherine M. Duclos, Audrey Champagne, Julie C. Carrier, Caroline Saucier, Christine L. Lavoie, Jean-Bernard Denault
CELL DEATH DISCOVERY
(2017)