Article
Biophysics
Miao Li, Seonik Lee, Maryam Zahedian, Chuanlin Ding, Jun Yan, Yan Yu
Summary: The crosstalk between FcyR and TLR2/1 receptors occurs through interface interactions between non-colocalizing receptor nanoclusters and is modulated by ligand mobility.
BIOPHYSICAL JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Miao He, Xiang Zhou, Zhigang Li, Xue Yin, Wenjie Han, Junxiang Zhou, Xiaoyun Sun, Xiaoyu Liu, Dongbao Yao, Haojun Liang
Summary: A multi-module CRISPR-dCas9 system with a separate structured RNA as a programmable component was developed for internal control over gene regulation. Compared to the traditionally used dCas9-VPR, this system showed higher activation efficiency. By incorporating a microRNA sensor, a transcriptional regulation platform responsive to endogenous microRNAs was created, enabling controllable activation of endogenous genes. Furthermore, the platform was successfully applied to selectively identify HCT116 cells in a cell mixture.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Shan She, Weimin Xuan, Nicola L. Bell, Robert Pow, Eduard Garrido Ribo, Zoe Sinclair, De-Liang Long, Leroy Cronin
Summary: This research utilizes oligopeptides as structure-directing ligands to modulate the self-assembly of POM superstructures via metal-coordination and hydrogen bonded interactions. It is found that changing the N-terminal amino acid on the peptide chain can readily switch the helicity of the nanowheel. Additionally, a delicate balance between Mo-coordination and hydrogen bonds within the internal cavity of the inorganic nanowheels leads to the sequence mediated formation of two unprecedented asymmetrical nanowheel frameworks: {Mo122Ce5} and {Mo126Ce4}.
Article
Multidisciplinary Sciences
Lorenzo Bonaguro, Jonas Schulte-Schrepping, Caterina Carraro, Laura L. Sun, Benedikt Reiz, Ioanna Gemund, Adem Saglam, Souad Rahmouni, Michel Georges, Peer Arts, Alexander Hoischen, Leo A. B. Joosten, Frank L. van de Veerdonk, Mihai G. Netea, Kristian Haendler, Sach Mukherjee, Thomas Ulas, Joachim L. Schultze, Anna C. Aschenbrenner
Summary: This study utilizes population-scale datasets to infer gene function and relationships between phenotypes and expression, successfully stratifying genes according to biological functions and predicting the importance of monocytes in the pathophysiology of a specific disease.
Article
Multidisciplinary Sciences
Fan Li, Hui Wang, Yan-Qi Li, Yebo Gu, Xin-Ming Jia
Summary: The study reveals that CLEC2D can form homodimers or heterodimers with TLR2, negatively regulating antifungal immunity by suppressing IRF5-mediated IL-12 production.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Zhaoming Wang, George J. Weiner
Summary: The study suggests that TIGIT and TIM3 can serve as markers for anti-CD20-mediated NK cell activation, but may not be suitable as targets for enhancing the anti-tumor activity of this therapy.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zeguang Wu, Soo Park, Colleen M. Lau, Yi Zhong, Sam Sheppard, Joseph C. Sun, Jayajit Das, Gregoire Altan-Bonnet, Katharine C. Hsu
Summary: Interactions between MHC on target cells and NK cell inhibitory receptors determine the abundance of SHP-1 in NK cells, with responsive NK cell populations having low SHP-1 levels. Reduction of SHP-1 abundance enhances NK cell responsiveness, suggesting it may be a biomarker for enhancing NK cell tumoricidal capacity.
Article
Neurosciences
Heather N. Allen, Sarah Chaudhry, Veronica M. Hong, Lakeisha A. Lewter, Ghanshyam P. Sinha, Yarimar Carrasquillo, Bradley K. Taylor, Benedict J. Kolber
Summary: The study identifies a parabrachial-to-amygdala circuit driven by opposing actions of CGRP, which determines the lateralization of visceral pain.
BIOLOGICAL PSYCHIATRY
(2023)
Article
Immunology
Tyler G. Normile, Timothy H. Chu, Brian S. Sheridan, Maurizio Del Poeta
Summary: The administration of the Cryptococcus neoformans Delta sgl1 mutant vaccine can protect mice from subsequent infection, even during CD4(+) T cell deficiency. IFN-gamma and IL-17A, produced by gamma delta T cells, play a crucial role in this protection mechanism. The interaction between sterylglucosides (SGs), GXM, and toll-like receptor 2 (TLR2) stimulates the production of IFN-gamma and IL-17A.
MUCOSAL IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Eduardo Moreo, Aitor Jarit-Cabanillas, Inaki Robles-Vera, Santiago Uranga, Claudia Guerrero, Ana Belen Gomez, Pablo Mata-Martinez, Luna Minute, Miguel Araujo-Voces, Maria Jose Felgueres, Gloria Esteso, Iratxe Uranga-Murillo, Maykel Arias, Julian Pardo, Carlos Martin, Mar Vales-Gomez, Carlos del Fresno, David Sancho, Nacho Aguilo
Summary: Intravenous administration of BCG shows potential in the treatment of lung tumors, reducing tumor growth and enhancing immune responses. It also enhances the efficacy of immune checkpoint blockade.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Wenqian Li, Miao Li, Stephen M. Anthony, Yan Yu
Summary: This study reveals that the signaling crosstalk between Fc gamma receptor and Toll-like receptor is dependent on the spatial proximity on phagosomes, with synergy increasing as receptors get closer and inhibitory effects from Fc gamma RIIb being constant regardless of receptor proximity. The overall cellular responses result from an integration of these two mechanisms.
SCIENTIFIC REPORTS
(2021)
Article
Genetics & Heredity
Hannes Vietzen, Vera Danklmaier, Alexander Zoufaly, Elisabeth Puchhammer-Stockl
Summary: This study investigates the role of genetic variants in activating natural killer (NK) cell receptors in contributing to the severity of COVID-19. The study shows that the high-affinity variant of the Fc gamma RIIIa receptor is over-represented in hospitalized and deceased patients with COVID-19, while the low-affinity variant is more common in patients with mild COVID-19. Additionally, functional assays reveal that hospitalized patients with COVID-19 exhibit significantly higher proinflammatory ADCC responses, particularly in NK cells expressing the high-affinity variant of the Fc gamma RIIIa receptor.
GENETICS IN MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
He Yang, Zijian Xiong, Xingyu Heng, Xiaomeng Niu, Yichen Wang, Lihua Yao, Lele Sun, Zhuang Liu, Hong Chen
Summary: Click-chemistry-mediated cell membrane glycopolymer engineering is used to potentiate dendritic cell vaccines (DCV). The glycopolymer-engineering facilitates the adhesion of dendritic cells to T cells, thereby augmenting T cell activation and improving tumor immunotherapy, providing a new strategy for designing enhanced dendritic cell-based therapeutics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biology
Takeshi Susukida, Saki Kuwahara, Binbin Song, Akira Kazaoka, Shigeki Aoki, Kousei Ito
Summary: Using a transgenic mouse model that recapitulates abacavir hypersensitivity syndrome, an idiosyncratic adverse drug reaction, Susukida et al show that individual differences in the immune tolerance system affect the susceptibility to idiosyncratic drug toxicity.
COMMUNICATIONS BIOLOGY
(2021)
Article
Oncology
Roberta Pascolutti, Lakshmi Yeturu, Geraldine Philippin, Stephane Costa Borges, Magali Dejob, Marie-Laure Santiago-Raber, Madiha Derouazi
Summary: This study validates the design of the self-adjuvanting ATP128 vaccine for cancer immunotherapy. ATP128 activates NF-kappa B and IRF3 signaling pathways to induce cellular immune response, and it can activate human dendritic cells through TLR2 and TLR4. This research is significant for the design of peptide-based cancer vaccines.
Review
Biochemistry & Molecular Biology
Takashi Ebihara, Ichiro Taniuchi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Multidisciplinary Sciences
Chizuko Miyamoto, Satoshi Kojo, Motoi Yamashita, Kazuyo Moro, Georges Lacaud, Katsuyuki Shiroguchi, Ichiro Taniuchi, Takashi Ebihara
NATURE COMMUNICATIONS
(2019)
Article
Chemistry, Organic
Shinsuke Inuki, Yukari Fujimoto
TETRAHEDRON LETTERS
(2019)
Article
Multidisciplinary Sciences
Amanda Ardain, Racquel Domingo-Gonzalez, Shibali Das, Samuel W. Kazer, Nicole C. Howard, Alveera Singh, Mushtaq Ahmed, Shepherd Nhamoyebonde, Javier Rangel-Moreno, Paul Ogongo, Lan Lu, Duran Ramsuran, Maria de la Luz Garcia-Hernandez, Tyler K. Ulland, Matthew Darby, Eugene Park, Farina Karim, Laura Melocchi, Rajhmun Madansein, Kaylesh Jay Dullabh, Micah Dunlap, Nancy Marin-Agudelo, Takashi Ebihara, Thumbi Ndung'u, Deepak Kaushal, Alexander S. Pym, Jay K. Kolls, Adrie Steyn, Joaquin Zuniga, William Horsnell, Wayne M. Yokoyama, Alex K. Shalek, Henrik N. Kloverpris, Marco Colonna, Alasdair Leslie, Shabaana A. Khader
Article
Chemistry, Organic
Shinsuke Inuki, Keisuke Sato, Naoto Zui, Ryosuke Yamaguchi, Takanori Matsumaru, Yukari Fujimoto
JOURNAL OF ORGANIC CHEMISTRY
(2019)
Review
Immunology
Takashi Ebihara, Ichiro Taniuchi
TRENDS IN IMMUNOLOGY
(2019)
Review
Cell Biology
Takashi Ebihara
Review
Allergy
Takashi Ebihara, Megumi Tatematsu, Akane Fuchimukai, Toshiki Yamada, Kenki Yamagata, Shunsuke Takasuga, Takechiyo Yamada
Summary: ILC2s reside in peripheral tissues and provoke innate type 2 immunity by producing T(H)2 cytokines. They can be trained by IL-33 or allergic inflammation, are long-lived, and mount memory-like immune responses. Additionally, IL-33, along with retinoic acid, induces immunosuppressive ILC2s producing IL-10.
ALLERGOLOGY INTERNATIONAL
(2021)
Article
Chemistry, Analytical
Mutsuo Nuriya, Yosuke Ashikari, Takanori Iino, Takuya Asai, Jingwen Shou, Keiko Karasawa, Kaho Nakamura, Yasuyuki Ozeki, Yukari Fujimoto, Masato Yasui
Summary: The development of alkyne-tagged dopamine compounds (ATDAs) provides a minimally invasive and uniquely identifiable tool to mimic dopamine in neurons and brain tissues, allowing for detailed characterization of dopamine dynamics. This approach can potentially be applied to other small-sized neurotransmitters as well, facilitating research on their dynamics in the brain.
ANALYTICAL CHEMISTRY
(2021)
Letter
Allergy
Yui Miyabe, Hiroki Tomizawa, Hidekazu Saito, Toshiki Yamada, Kazuhiro Shiina, Koh Koizumi, Yohei Kawasaki, Shinsuke Suzuki, Mineyo Fukuchi, Shigeharu Ueki, Takashi Ebihara, Takechiyo Yamada
Article
Biochemistry & Molecular Biology
Jianbo An, Yushi Nagaki, Satoru Motoyama, Yuta Kuze, Midori Hoshizaki, Kohei Kemuriyama, Tomokazu Yamaguchi, Takashi Ebihara, Yoshihiro Minamiya, Yutaka Suzuki, Yumiko Imai, Keiji Kuba
Summary: Metastasis predicts poor prognosis in cancer patients. Galectin-7 is identified as a crucial mediator of metastasis associated with immunosuppression. Galectin-7 is expressed in the immunosuppressive tumor microenvironment and plays an important role in tumor metastasis to lymph nodes and lungs.
Article
Immunology
Toshiki Yamada, Megumi Tatematsu, Shunsuke Takasuga, Akane Fuchimukai, Kenki Yamagata, Shinsuke Seki, Keiji Kuba, Hideyuki Yoshida, Ichiro Taniuchi, Gunter Bernhardt, Kazuko Shibuya, Akira Shibuya, Takechiyo Yamada, Takashi Ebihara
Summary: While group-2 innate lymphoid cells (ILC2s) are highly proliferative in allergic inflammation, the removal of overactivated ILC2s in allergic diseases has not been investigated. We previously showed that chronic airway allergy induces exhausted-like dysfunctional ILC2s expressing TIGIT. Chronic allergy stably induced TIGIT(+) ILC2s, which were highly activated, apoptotic, and were quickly removed from sites of chronic allergy. Our work demonstrates that TIGIT shifts the fate of ILC2s toward activation-induced cell death, which could present a new therapeutic target for chronic allergies.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Tsung-Che Chang, Yoshiyuki Manabe, Keita Ito, Ryuku Yamamoto, Kazuya Kabayama, Shino Ohshima, Yoshie Kametani, Yukari Fujimoto, Chun-Cheng Lin, Koichi Fukase
Summary: Sialyl-Tn (STn), a tumor-associated antigen, has been investigated as a potential target for anti-cancer vaccine therapy. In this study, a self-adjuvanting STn-based vaccine was developed, consisting of a clustered STn antigen, a TLR1/2 ligand, and a T-helper cell epitope. This three-component vaccine was found to effectively induce the production of anti-STn antibodies. The immune responses induced by this vaccine were analyzed in detail, revealing the importance of TLR1/2 recognition and T-helper cell activation in antibody production.
Article
Chemistry, Organic
Yohei Arai, Shota Torigoe, Takanori Matsumaru, Sho Yamasaki, Yukari Fujimoto
ORGANIC & BIOMOLECULAR CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Takanori Matsumaru, Risa Ikeno, Yusuke Shuchi, Toshiki Iwamatsu, Takashi Tadokoro, Sho Yamasaki, Yukari Fujimoto, Atsushi Furukawa, Katsumi Maenaka
CHEMICAL COMMUNICATIONS
(2019)