Article
Environmental Sciences
Abrar Muhammad, Xiaoxia Zhou, Jintao He, Nan Zhang, Xiaoqiang Shen, Chao Sun, Bing Yan, Yongqi Shao
Summary: The study found that exposure to polystyrene microplastics and nanoplastics had different effects on silkworms. Microplastics induced a stronger immune response, while nanoplastics had more of an inhibitory response. Silkworms exposed to microplastics showed better survival rates when infected with pathogens, while those exposed to nanoplastics had significantly higher mortality.
ENVIRONMENTAL POLLUTION
(2021)
Article
Environmental Sciences
Qi Ge, Weiping Cao, Feifei Zhu, Yi Yuan, Liang Chen, Jia Xu, Jun Li, Han Chen, Shangshang Ma, Lindan Sun, Huiwen Pan, Rehab Hosny Taha, Qin Yao, Keping Chen
Summary: The study revealed that Bacillus cereus ZJ-4 isolated from hospital environment can lead to death in silkworms, causing pathological changes in fat body tissue and affecting the immune pathway and immune function of silkworms.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemical Research Methods
Feifei Zhu, Dandan Song, Han Chen, Qi Tang, Shuhao Huo, Xiaoyong Liu, Keping Chen
Summary: Lipids have been suggested to play a crucial role in virus entry and infection. A comprehensive lipidomic analysis using UPLC-MS was conducted on susceptible and resistant silkworm strains, revealing key lipids associated with virus infection and immune response. The study highlights the relationship between lipid biosynthesis, viral resistance, and immune response in the host.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Entomology
Kakeru Yokoi, Takuya Tsubota, Akiya Jouraku, Hideki Sezutsu, Hidemasa Bono
Summary: In this study, reference transcriptome data for the silkworm was established and detailed examination of gene expression in silkworm tissues was performed. The results contribute to understanding silkworm biology and promoting industrial applications.
Review
Physiology
Mian Muhammad Awais, Muhammad Shakeel, Jingchen Sun
Summary: miRNAs are small non-coding RNAs of about 22 nucleotides that have been reported to regulate gene expression at the posttranscriptional level, involved in various biological processes such as immunity, development, metabolism, and host-pathogen interactions. Both cellular and viral miRNAs may play vital roles in host-pathogen interactions, with viral infections altering host miRNA expression profiles.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biology
Shuangzhi Yuan, Yong Sun, Wenqiang Chang, Jiaozhen Zhang, Jifa Sang, Jiachun Zhao, Minghui Song, Yanan Qiao, Chunyang Zhang, Mingzhu Zhu, Yajie Tang, Hongxiang Lou
Summary: Herbivores have evolved detoxification mechanisms for feed components. Silkworms that feed on Cudrania tricuspidata leaves (CTLs) have smaller bodies, slower growth, and lower silk production compared to those fed mulberry leaves. The high content of prenylated isoflavones (PIFs) in CTLs is converted into glycosylated derivatives (GPIFs) in silkworm faeces through gut microbiota, and this biotransformation is essential for PIFs detoxification. Adding Bacillus subtilis as a probiotic can improve gut microbiota and promote silkworm growth and development.
COMMUNICATIONS BIOLOGY
(2023)
Article
Entomology
Hao Sun, Jingya Chen, Ruolin Wang, Dan Liu, Na Zhang, Tong Zhang, Ling Jia, Sanyuan Ma, Qingyou Xia
Summary: This study used a genome-wide CRISPR/Cas9-based library and large-scale screening to analyze the key genes involved in the silkworm 20E signaling pathway and their mechanisms of action. Functional annotation revealed that 20E regulates key proteins in processes mainly occurring in the cytoplasm and nucleus. Pathway enrichment analysis showed that 20E can activate phosphorylation, may affect innate immunity, interfere with intracellular nutrition and energy metabolism, and ultimately lead to cell apoptosis. These findings provide a panoramic overview of the silkworm's response to 20E signaling, highlighting the utility of genome-wide CRISPR mutant libraries in deciphering hormone signaling pathways and metamorphosis regulation in insects.
Article
Entomology
Kohei Kakino, Hiroaki Mon, Takeru Ebihara, Masato Hino, Akitsu Masuda, Jae Man Lee, Takahiro Kusakabe
Summary: This study characterizes the gene expression patterns and function in the testis of Bombyx mori, revealing peculiar features in spermatogenesis. The transcriptome analysis identifies widespread gene expression and testis-specific genes in B. mori, and compares it with Drosophila melanogaster for a better understanding. The study fills a knowledge gap and provides a basis for future research in spermatogenesis.
Article
Multidisciplinary Sciences
Linrong Wan, Wenfu Xiao, Ziyan Huang, Anlian Zhou, Yaming Jiang, Bangxing Zou, Binbin Liu, Cao Deng, Youhong Zhang
Summary: This article systematically identified and analyzed small open reading frames (smORFs) in the silkworm, finding 738 highly reliable smORFs and 34,401 possible smORFs. It also identified differentially expressed and tissue-specific-expressed smORFs, which may be closely related to tissue characteristics and functions.
Article
Chemistry, Multidisciplinary
Lin Ma, Vivian Andoh, Zhongyuan Shen, Haiyan Liu, Long Li, Keping Chen
Summary: This study aimed to explore the long-term effects of magnesium oxide nanoparticles (MgO NPs) on silkworms. The results showed that MgO NPs had no significant negative impact on silkworm growth or adult tissues, but altered the expression of genes in the silk gland.
Article
Biochemistry & Molecular Biology
Pooja Makwana, Shambhavi P. Hungund, Appukuttan Nair R. Pradeep
Summary: This study identified the mechanisms by which the Dipteran endoparasitoid E. bombycis inhibits host defense reactions by exploiting antihemocyte activity. The peptides of hemocyte aggregation inhibitor protein (HAIP) and pyridoxamine phosphate oxidase (PNPO) were found to be involved in the process. These proteins showed increased expression in the integument of the parasitoid larva, further enhancing its virulence.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Environmental Sciences
Shoumin Sun, Qiqi Chen, Gang Chen, Zhenzhen Chen, Kaiyun Wang, Hongyan Wang
Summary: In this study, a risk assessment of the neonicotinoid insecticide nitenpyram was conducted in relation to its impact on silkworms. The study confirmed the presence of risks when nitenpyram is sprayed around mulberry fields. Additionally, differentially expressed miRNAs and related signaling pathways were identified, indicating the potential mechanism underlying nitenpyram's toxic effect on silkworms. Moreover, nitenpyram was found to induce oxidative damage and upregulate Imd, suggesting its potential impact on the growth and stress response of silkworms.
Article
Food Science & Technology
Maria Cermeno, Carmen Bascon, Miryam Amigo-Benavent, Manuel Felix, Richard J. FitzGerald
Summary: Silkworm pupae hydrolysates exhibited significant antioxidant activity, with Alcalase and Prolyve hydrolysates having the highest scavenging activities, and Flavourzyme and Brewers Clarex hydrolysates showing enhanced ferric reducing antioxidant power. Peptides SWFVTPF and NDVLFF demonstrated the highest antioxidant activity in HepG2 cells.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biochemistry & Molecular Biology
Zhi-hao Su, Jun-li Lv, Qi Ou, Zi-qin Zhao, Kai-yi Zheng, Xiao-ying Zhang, Wen-qing Lai, Xue-yang Wang, Ming-jie Deng, Mu-wang Li
Summary: The white epidermis of silkworms is caused by uric acid crystals accumulation. Abnormal metabolism of uric acid reduces its production, resulting in a transparent or translucent phenotype. This study examined the metabolite changes following Bombyx mori nucleopolyhedrovirus (BmNPV) infection in two strains of silkworms (p50 and op50) and found that the uric acid pathway plays a critical role in resistance. Feeding with inosine, a metabolite in this pathway, significantly enhanced larval resistance and modulated other metabolic pathways. The results provide insights into the resistance mechanism of silkworms to BmNPV and offer new strategies for pest control.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Physiology
Min-Li Dai, Wen-Tao Ye, Xue-Jian Jiang, Piao Feng, Qing-Yu Zhu, Hai-Na Sun, Fan-Chi Li, Jing Wei, Bing Li
Summary: This study investigated the effects of the generalist parasitoid Exorista japonica on the biological traits of its lepidopteran host, Bombyx mori. It was found that parasitism by E. japonica shortened the molting duration and decreased the emergence rate of the host, while also affecting the hormone levels and gene expression related to molting and pupation. These findings highlight a novel target mechanism employed by tachinid parasitoids and provide new insights into host/parasitoid relationships.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Liang Jiang, Chunlin Huang, Qiang Sun, Huizhen Guo, Tingcai Cheng, Zhengwen Peng, Yinghui Dang, Weiqiang Liu, Guowen Xu, Qingyou Xia
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2015)
Article
Entomology
Xiao-Ting Hua, Xiao-Juan Ma, Ren-Ju Xue, Ting-Cai Cheng, Fei Wang, Qing-You Xia
Article
Biochemistry & Molecular Biology
Wenbo Hu, Chun Liu, Tingcai Cheng, Wei Li, Niannian Wang, Qingyou Xia
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2016)
Article
Entomology
Ting-Cai Cheng, Ren-Wen Long, Yu-Qian Wu, You-Bing Guo, Duo-Lian Liu, Li Peng, Dai-Qin Li, Dai-Wen Yang, Xin Xu, Feng-Xiang Liu, Qing-You Xia
Article
Entomology
Tingcai Cheng, Ping Lin, Lulin Huang, Yuqian Wu, Shengkai Jin, Chun Liu, Qingyou Xia
JOURNAL OF INSECT SCIENCE
(2016)
Article
Biochemistry & Molecular Biology
Wei Li, Tingcai Cheng, Wenbo Hu, Zhangchuan Peng, Chun Liu, Qingyou Xia
MOLECULAR GENETICS AND GENOMICS
(2016)
Article
Microbiology
Ping Lin, Tingcai Cheng, Sanyuan Ma, Junping Gao, Shengkai Jin, Liang Jiang, Qingyou Xia
Article
Biochemistry & Molecular Biology
Huizhen Guo, Tingcai Cheng, Zhiwei Chen, Liang Jiang, Youbing Guo, Jianqiu Liu, Shenglong Li, Kiyoko Taniai, Kiyoshi Asaoka, Keiko Kadono-Okuda, Kallare P. Arunkumar, Jiaqi Wu, Hirohisa Kishino, Huijie Zhang, Rakesh K. Seth, Karumathil P. Gopinathan, Nicolas Montagne, Emmanuelle Jacquin-Joly, Marian R. Goldsmith, Qingyou Xia, Kazuei Mita
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Chun Liu, Wenbo Hu, Tingcai Cheng, Zhangchuan Peng, Kazuei Mita, Qingyou Xia
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Huaiyan Gan, Tieshan Feng, Yuqian Wu, Chun Liu, Qingyou Xia, Tingcai Cheng
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Xiao-Ming Zhao, Chun Liu, Li-Jun Jiang, Qiong-Yan Li, Meng-Ting Zhou, Ting-Cai Cheng, Kazuei Mita, Qing-You Xia
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Plant Sciences
Xiaodong Xie, Genhong Wang, Ling Yang, Tingcai Cheng, Junping Gao, Yuqian Wu, Qingyou Xia
PHYSIOLOGIA PLANTARUM
(2015)
Article
Multidisciplinary Sciences
Yuqian Wu, Tingcai Cheng, Chun Liu, Duolian Liu, Quan Zhang, Renwen Long, Ping Zhao, Qingyou Xia
Article
Multidisciplinary Sciences
Liang Jiang, Zhengwen Peng, Youbing Guo, Tingcai Cheng, Huizhen Guo, Qiang Sun, Chunlin Huang, Ping Zhao, Qingyou Xia
SCIENTIFIC REPORTS
(2016)
Article
Biochemistry & Molecular Biology
Tingcai Cheng, Xia Zhang, Zhangchuan Peng, Yinfeng Fan, Lin Zhang, Chun Liu
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)