Article
Virology
Naoko Uno, Ted M. Ross
Summary: The study developed four independent DENV envelope glycoproteins using COBRA methodology to induce broadly neutralizing antibodies against all four serotypes, demonstrating a broad protective immune response in rhesus macaques.
JOURNAL OF VIROLOGY
(2021)
Article
Chemistry, Medicinal
Tommaso Felicetti, Chin Piaw Gwee, Maria Sole Burali, Kitti Wing Ki Chan, Sylvie Alonso, Maria Chiara Pismataro, Stefano Sabatini, Maria Letizia Barreca, Violetta Cecchetti, Subhash G. Vasudevan, Giuseppe Manfroni
Summary: Researchers designed and synthesized a series of sulfonyl anthranilic acid (SAA) derivatives that showed potential inhibition against dengue virus (DENV) infection in cells. These derivatives did not affect the activity of DENV NS5 polymerase. Compounds 26 and 39 exhibited the best inhibitory effects, with EC50 values in the range of 0.54-1.36 μM against cells infected with all four dengue serotypes. The study suggests that SAA derivatives could serve as a valuable starting point for the development of effective antiviral therapeutics for dengue.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Gastroenterology & Hepatology
Lung-Yi Mak, Rex Wan-Hin Hui, Wai-Kay Seto, Man-Fung Yuen
Summary: NAPs and CAMs are two types of virus-directing therapies for chronic hepatitis B, with different efficacy and applicability. NAPs can achieve functional cure in some patients when used in combination with other drugs, while CAMs can suppress viral nucleic acids but only have modest effects on HBsAg suppression. Neither compound is likely to be a stand-alone treatment for functional cure, and further evaluation is needed in different populations of chronic hepatitis B.
CLINICS IN LIVER DISEASE
(2023)
Article
Virology
Rong Wang, Jiansheng Lu, Lei Chen, Yunzhou Yu, Zhixin Yang
Summary: In this study, a bispecific antibody LUZ-8F2?6B1 was engineered to display potent neutralization activity against four serotypes of DENV, providing protection in a mouse model. Additionally, deletion of nine amino acids in the Fc region abolished antibody-dependent enhancement at lower doses, making LUZ-8F2?6B1 a promising, safe, and effective agent for prophylaxis and treatment of DENV infection.
Article
Microbiology
Ritesh Panchal, Saikat Ghosh, Rajeev Mehla, Jayachandran Ramalingam, Sunil Gairola, Sandeepan Mukherjee, Abhay Chowdhary
Summary: This study evaluated the antiviral activity of Rosmarinic acid (RA) against four serotypes of dengue virus (DENV). The results showed that RA could bind to DENV proteins and inhibit infection in cell culture. However, the effectiveness of RA varied among different serotypes.
CURRENT MICROBIOLOGY
(2022)
Article
Microbiology
Nina M. Pollak, Malin Olsson, Madeeha Ahmed, Javier Tan, George Lim, Yin Xiang Setoh, Judith Chui Ching Wong, Yee Ling Lai, Jody Hobson-Peters, Joanne Macdonald, David McMillan
Summary: Efficient and accurate diagnosis of dengue is crucial for clinical care, surveillance, and outbreak control. This study evaluates four rapid low-resource tests for specific dengue virus serotyping, using simple sample preparation and RT-RPA combined with LFD technology. These tests provide advantages such as a simple workflow, rapid turnaround time (35 min), minimal equipment needs, and improved laboratory safety. The low-cost formats of these tests have the potential to support effective dengue disease surveillance and enhance diagnostic testing capacity in resource-limited settings.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Charlotte Baliere, Elodie Calvez, Jean-Michel Thiberge, Somphavanh Somlor, Mathias Vandenbogaert, Marc Grandadam, Valerie Caro
Summary: Dengue fever is the most prevalent arthropod-borne viral infection in tropical and subtropical countries. Through sequencing samples from five provinces in Laos, the study explored the co-circulation of dengue viruses and suggested the emergence or re-emergence of new strains potentially linked to exchanges with neighboring countries. The surveillance and alert system created by the Institut Pasteur du Laos in Vientiane capital appears to be a strategic tool for monitoring dengue circulation and improving epidemiological knowledge to anticipate epidemic events.
Article
Immunology
Pragati Sharma, Kaustuv Nayak, Elluri Seetharami Reddy, Humaira Farooqi, Kaja Murali-Krishna, Anmol Chandele
Summary: Dengue is a significant global public health issue, with neutralizing antibody responses being crucial for vaccine development and evaluation. The study found that optimizing a flow cytometry-based neutralization assay resulted in higher sensitivity and concordance with other testing methods.
Article
Infectious Diseases
Praveen Rai, Sonam Kille, Akshatha Kotian, Ballamoole Krishna Kumar, Vijaya Kumar Deekshit, Mysore Shivalingappa Ramakrishna, Indrani Karunasagar, Iddya Karunasagar
Summary: The study examined the circulating dengue virus and serotypes in patients in Mysuru district, Southern India during disease outbreak. Results showed DENV-4 as the predominant serotype in 2016, while DENV-2, DENV-3, and DENV-1 emerged in 2017. The study highlights the importance of genetic diversity in monitoring and controlling dengue infection.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Multidisciplinary Sciences
Sthita Pragnya Behera, Pooja Bhardwaj, Hirawati Deval, Neha Srivastava, Rajeev Singh, Brij Ranjan Misra, Awdhesh Agrawal, Asif Kavathekar, Rajni Kant
Summary: This study genetically characterized the circulating dengue virus (DENV) serotypes among patients admitted for dengue in the Eastern Uttar Pradesh (E-UP) region during 2018-2019. The results showed the circulation of all four DENV1-4 serotypes, with DENV-2 being the most abundant. This study highlights the importance of continuous molecular surveillance for monitoring circulating DENV serotypes.
Article
Engineering, Biomedical
Beom Jeung Hwang, Yohan Jang, Soon Bin Kwon, Ji Eun Yu, Jongkwan Lim, Young Hoon Roh, Baik L. Seong
Summary: Virus-like particles (VLPs) represent a high-priority vaccine platform against emerging viral infections, with RNA and tRNA playing crucial roles in protein folding and antigen assembly.
Article
Biodiversity Conservation
Elisa Helena Paz Andrade, Leandra Barcelos Figueiredo, Ana Paula Pessoa Vilela, Julio Cesar Camara Rosa, Hassan Melo Zibaoui, Erna Geessien Kroon
Summary: This study identified the co-circulation of all four dengue virus serotypes in Aedes aegypti mosquitoes in a hyperendemic area in Brazil, highlighting the challenge for disease control and the importance of virological surveillance in predicting circulating dengue virus serotypes in humans.
Article
Virology
Jing Zhang, Yongxiang Wang, Shuwen Fu, Quan Yuan, Qianru Wang, Ningshao Xia, Yumei Wen, Jisu Li, Shuping Tong
Summary: The study showed that full-length S protein sustains intracellular and extracellular L and M proteins, while truncated S protein blocks subviral particle secretion.
Article
Multidisciplinary Sciences
Nathane C. Mebus-Antunes, Wellington S. Ferreira, Glauce M. Barbosa, Thais C. Neves-Martins, Gilberto Weissmuller, Fabio C. L. Almeida, Andrea T. Da Poian
Summary: This study establishes the conditions for in vitro assembly of DENV nucleocapsid-like particles (NCLPs) using recombinant DENVC. The assembly process is concentration-dependent and involves DENVC charge neutralization. The results shed light on the assembly mechanism of DENV and have important implications for understanding the virus.
Article
Virology
Jiansheng Lu, Lei Chen, Peng Du, Jiazheng Guo, Xi Wang, Yujia Jiang, Yunzhou Yu, Rong Wang, Zhixin Yang
Summary: This study found that HMAb 9C7 can neutralize all four serotypes of DENV and has activity in both the attachment and post-attachment steps of the virus lifecycle for DENV-1. Additionally, HMAb 9C7 has the ability to protect suckling mice from DENV challenge.
Review
Virology
Zhao-Yang Wang, Pei-Gang Wang, Jing An
Summary: The TAM receptors have various roles in maintaining immune and inflammatory homeostasis, promoting virus infection, and protecting against neuroinvasive viral infections in mice. Research suggests that TAM receptors not only protect mice from the effects of neuroinvasive virus infections, but also have an impact on the outcome of virus infections through multiple mechanisms.
Letter
Virology
Jia-Yuan Shen, Man Li, Lyu Xie, Jia-Rong Mao, Hong-Ning Zhou, Pei-Gang Wang, Jin-Yong Jiang, Jing An
Article
Immunology
Praveen M. Varghese, Valarmathy Murugaiah, Nazar Beirag, Nigel Temperton, Haseeb A. Khan, Salman H. Alrokayan, Mohammed N. Al-Ahdal, Beatrice Nal, Futwan A. Al-Mohanna, Robert B. Sim, Uday Kishore
Summary: C4BP binds to different subtypes of Influenza A Virus and modulates the inflammatory response, showing differential effects on H1N1 and H3N2. It inhibits viral entry for H1N1 but promotes infection by H3N2, also regulating the expression of pro-inflammatory cytokines in a subtype-dependent manner.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Li-Bo Liu, Man Li, Na Gao, Jia-Yuan Shen, Zi-Yang Sheng, Dong-Ying Fan, Hong-Ning Zhou, Xiao-Xiong Yin, Jia-Rong Mao, Jin-Yong Jiang, Pei-Gang Wang, Jing An
Summary: An outbreak of Chikungunya fever occurred in Ruili, Yunnan Province from September to December 2019, with a total of 112 confirmed cases, including 91 local cases and 21 imported cases. Female patients outnumbered male patients, and common symptoms included joint pain, fever, fatigue, chills, rash, and headache. Phylogenetic analysis revealed that the CHIKV strains in this outbreak belonged to the Indian Ocean clade of the East/Central/South African genotype.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Pathology
Hui-Ling Yen, Sophie Valkenburg, Sin Fun Sia, Ka Tim Choy, J. S. Malik Peiris, Karen H. M. Wong, Nicholas Crossland, Florian Douam, John M. Nicholls
Summary: Animal models, such as Syrian hamsters and K18-hACE2 transgenic mice, show different pathologic changes in response to SARS-CoV-2 infection. Syrian hamsters are useful for studying transmission and vaccination strategies, while the transgenic mouse model exhibits fatal neuroinvasion without involvement of the conducting airways.
VETERINARY PATHOLOGY
(2022)
Article
Immunology
Z. Y. Sheng, M. Li, R. Yang, Y. H. Liu, X. X. Yin, J. R. Mao, Heidi E. Brown, J. An, H. N. Zhou, P. G. Wang
Summary: The measures taken during the COVID-19 pandemic were effective in preventing the transmission of dengue, particularly between cities and from urban to suburban areas. However, once a dengue outbreak was established, its distribution became stable and resistant to control efforts.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Pharmacology & Pharmacy
Martin Sachse, Raquel Tenorio, Isabel Fernandez de Castro, Jordana Munoz-Basagoiti, Daniel Perez-Zsolt, Dalia Raich-Regu, Jordi Rodon, Alejandro Losada, Pablo Avil, Carmen Cuevas, Roger Paredes, Joaquim Segales, Bonaventura Clotet, Julia Vergara-Alert, Nuria Izquierdo-Useros, Cristina Risco
Summary: The need for broad-spectrum, efficient antiviral treatments to combat emerging and re-emerging viruses has been highlighted by the COVID-19 pandemic. This study demonstrates that Plitidepsin, an antitumor agent, can effectively inhibit SARS-CoV-2 infection by disrupting the replication and morphogenesis of the virus.
ANTIVIRAL RESEARCH
(2022)
Article
Immunology
Yi-Song Wang, Dong Liu, Xin Wang, Qiao-Li Luo, Ling Ding, Dong-Ying Fan, Qi-Liang Cai, Chong-Yang Tang, Wei Yang, Yu-Guang Guan, Tian-Fu Li, Pei-Gang Wang, Guo-Ming Luan, Jing An
Summary: This study compared the HHV infection and immune response in brain tissues of Rasmussen's encephalitis (RE), temporal lobe epilepsy (TLE), and traumatic brain injury (TBI) patients. The results showed that HHV antigens were prevalent in both RE and TLE brain tissues, and CD8+ T cell infiltration was observed in both conditions. However, CD8+ T cells were only activated and showed apoptosis in RE. In comparison to TLE, RE brain tissues had higher levels of inflammatory cytokines but lower levels of interferon-beta, which was negatively correlated with HHV infection. The DNA sensors STING and IFI16 were insufficiently activated in RE.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Microbiology
Paula Ortega-Gonzalez, Gwen Taylor, Rohit K. Jangra, Raquel Tenorio, Isabel Fernandez de Castro, Bernardo A. Mainou, Robert C. Orchard, Craig B. Wilen, Pamela H. Brigleb, Jorna Sojati, Kartik Chandran, Martin Sachse, Cristina Risco, Terence S. Dermody
Summary: Cholesterol homeostasis, facilitated by NPC1, is required for reovirus penetration into the cytoplasm. This discovery suggests a new function for NPC1 and cholesterol homeostasis in viral infection, and raises the possibility of cholesterol regulating the entry of other viruses.
Article
Multidisciplinary Sciences
Yu Wu, Nassim Mahtal, Elea Paillares, Lea Swistak, Sara Sagadiev, Mridu Acharya, Caroline Demeret, Sylvie Van der Werf, Florence Guivel-Benhassine, Olivier Schwartz, Serena Petracchini, Amel Mettouchi, Lucie Caramelle, Pierre Couvineau, Robert Thai, Peggy Barbe, Mathilde Keck, Priscille Brodin, Arnaud Machelart, Valentin Sencio, Francois Trottein, Martin Sachse, Gaetan Chicanne, Bernard Payrastre, Florian Ville, Victor Kreis, Michel-Robert Popoff, Ludger Johannes, Jean-Christophe Cintrat, Julien Barbier, Daniel Gillet, Emmanuel Lemichez
Summary: The compound C910 has been identified to disrupt toxin transport pathways, protecting cells against various bacterial toxins and reducing influenza and SARS-CoV-2 viral infections.
Article
Multidisciplinary Sciences
Charlotte Calvet, Thibault Peineau, Najate Benamer, Maxence Cornille, Andrea Lelli, Baptiste Plion, Ghizlene Lahlou, Julia Fanchette, Sylvie Nouaille, Jacques Boutet de Monvel, Amrit Estivalet, Philippe Jean, Vincent Michel, Martin Sachse, Nicolas Michalski, Paul Avan, Christine Petit, Didier Dulon, Saaid Safieddine
Summary: This study found that SNAP-25 is essential for normal hearing function by ensuring exocytosis and maintenance of ribbon synapses in IHCs.
Article
Multidisciplinary Sciences
Wei Yang, Li-Bo Liu, Feng-Liang Liu, Yan-Hua Wu, Zi-Da Zhen, Dong-Ying Fan, Zi-Yang Sheng, Zheng-Ran Song, Jia-Tong Chang, Yong-Tang Zheng, Jing An, Pei-Gang Wang
Summary: Through single-cell RNA sequencing of ZIKV-infected mouse testes, it is found that spermatogenic cells, especially spermatogonia, are vulnerable to ZIKV infection. The complement system genes are significantly upregulated in infiltrated S100A4 + monocytes/macrophages. Complement activation and testicular damage are verified in ZIKV-infected macaques and the complement inhibitor C1INH and S100A4 inhibitors sulindac and niclosamide are tested. C1INH alleviates testicular pathology but worsens ZIKV infection, while niclosamide effectively reduces infiltration, inhibits complement activation, alleviates testicular damage, and improves fertility. This discovery highlights the importance of male reproductive health protection during future ZIKV epidemics.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Remy Char, Zhuangzhuang Liu, Cedric Jacqueline, Marion Davieau, Maria-Graciela Delgado, Clara Soufflet, Mathieu Fallet, Lionel Chasson, Raphael Chapuy, Voahirana Camosseto, Eva Strock, Rejane Rua, Catarina R. Almeida, Bing Su, Ana-Maria Lennon-Dumenil, Beatrice Nal, Antoine Roquilly, Yinming Liang, Stephane Meresse, Evelina Gatti, Philippe Pierre
Summary: This study shows that iRUFY3 plays a role in perinuclear positioning, phagocyte migration and antigen presentation during Salmonella infection. iRUFY3 controls macrophages migration, MHC II presentation and responses to Interferon-γ, while being important for intracellular Salmonella replication.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Thierry Mourer, Martin Sachse, Anastasia D. Gazi, Christophe d'Enfert, Sophie Bachellier-Bassi
Summary: This protocol presents methods for analyzing the ultrastructure of Candida albicans biofilm using transmission electron microscopy. It includes two approaches to observe fungal cells within unperturbed biofilms and an immunogold labeling procedure. By growing the biofilm on a plastic surface, the architecture of the fungal biofilm can be maintained close to its natural state. Classic transmission electron microscopy or electron tomography can then be used to analyze the microbial community's ultrastructure.
Correction
Immunology
Daniel Perez-Zsolt, Jordana Munoz-Basagoiti, Jordi Rodon, Marc Elosua-Bayes, Dalia Raich-Regue, Cristina Risco, Martin Sachse, Maria Pino, Sanjeev Gumber, Mirko Paiardini, Jakub Chojnacki, Itziar Erkizia, Xabier Muniz-Trabudua, Ester Ballana, Eva Riveira-Munoz, Marc Noguera-Julian, Roger Paredes, Benjamin Trinite, Ferran Tarres-Freixas, Ignacio Blanco, Victor Guallar, Jorge Carrillo, Julia Blanco, Amalio Telenti, Holger Heyn, Joaquim Segales, Bonaventura Clotet, Javier Martinez-Picado, Julia Vergara-Alert, Nuria Izquierdo-Useros
CELLULAR & MOLECULAR IMMUNOLOGY
(2022)